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The map label for this gene is dosC [H]

Identifier: 86751232

GI number: 86751232

Start: 4696164

End: 4697942

Strand: Reverse

Name: dosC [H]

Synonym: RPB_4125

Alternate gene names: 86751232

Gene position: 4697942-4696164 (Counterclockwise)

Preceding gene: 86751233

Following gene: 86751231

Centisome position: 88.11

GC content: 65.54

Gene sequence:

>1779_bases
ATGACACGAAACACGATGCGCGCTCTCTGGAATCAGATCGCCACCAGTCTCAAGACGAAATTTCTGGTCGTGACGATCTG
CGTGTTCGGCCTGGTCGCCCTGCCGGCCTATGCGGCGTTCGAATTCCTCACCCGCAAGACCGTGGTCGCGCTCGGCACGC
TGTTCGCCGAGAAGCAGGTGCTGTTCGATCGCTACCGCGGCCTCGAGGCGCTGATCCGCGAGGTCTCGTTGGCCGAGACG
GTCGCCCGCTCGCCGGCGGTGCGCGCCTGGGCCCGCGACGAGGCCGACCCCGACAAGACGGTGCGCGGGCTGGCCGAACT
CGAGCACTACCGGCTGTCGTTTCACGACAAGAGCTATTTCTTCGTCGTGCACGAGTCGGGCAATTACTACTTCAACGATG
CCGACAACAATTACAACGGCAACCAGCTCCGCTATCAGGTCAAGCGCGACAATTCGCGCGACGGCTGGTACTTCAAGACG
ATCGCGGCCGGTCAGGCCTGCCAGCTCAACGTCGACCGCGACGACAATCTCGCCGTCACCAAAGTCTGGATCAATTGCGT
CATCACCGATCAGGGCCGGATTCTCGGCGTGATCGGCACCGGCGTCGACCTCAGCGAATTCATCCGCGAGGTGGTGGCGT
TGCCGCAGACCGGCATCACCAGCATGTTCGTCGATCGCGCCGGCGCCATCCAGGCGCACCGCGATCCGGGGATGGTCGAT
TTCCACAGCCTCACCAAGGACACCAAGACCAAGAAGACGATCTTCCAGCAGGTCGACAAGCCGGCCGAGGCCGGCGAACT
CGCCGCCATGATGGCGCGCGTCTCCGCCGGCTCCAGGATGGTCGAATCGCGCTTCATGAACATCGGCGGGCGCGAATTCC
TGGTCGGTGTCGGCTATCTCGATCGGCTCGGCTGGTTCAACGTGACGTTGATGGACATCGACGCCATCATCGATCGCCGG
CTGTTCACGCCGATCGCGCTGCTGCTGGTGTTCATGATGACCGCGGCGGTCGCGCTCGTCACCATCCTGTTCCGGCGCGT
CGTGCTGGATCGGCTGGCGATCGTCGAGGCGGCCGCGCGACGGGTCGAAGACGGCGATTTCGACAATATCGTGGTCGATC
GCGGCAGCGACGAGATCGGCCGGCTGTCGCGGACGTTGTCCGGCATGGCGCATGCGGTGCGCGACCACACCCGCATTCTC
GAGGATCTGGTCGACGAGCGAACCGAGAAGCTGCGGCGTCTCGCCAATATCGATCTCTTGACCGAGATCCCCAACCGGCG
CGGTTTCACCCAGGTGTTCGAGCAGTGGCTGGCCAGGTCGCCTCTGGTGCCGCGGTCGGCCGGGCTGCTGCTGATCGATC
TGGATGACTTCAAGGACGTCAACGACGCGTTCGGCCATCTGGCCGGCGATCGCCTGCTGTGCGAGACCGCGCGGCGGCTG
GCGCCGCTCCTCACCGACAGGGACATCTGCGCCCGCTGGGGCGGCGACGAGTTCGTCGTGCTGGTCGAGGATTGCGACCA
GGACGGCCTGCGGGCGCTGTGCGCCCGCATGGTCGCCGCGATGAACGCCACGCCGATCCGCTCCGACGATGGTCAGGACA
TTGCGCTGACGGTCAGCATCGGCGGCTGTCGGGTGGTTTCGGGCAGTTCGATCGACGCCGCCTTCGCGGAGGCCGATGCA
GCGCTCTACGCCGCCAAGCGCGCCGGTCGCAACCGGTTCGTGCTGTTCGACGCCGACGCCGGATCGGCGCTCGACGCGGT
GGTGACGGCCAAGGCCTGA

Upstream 100 bases:

>100_bases
CGTAAACCATTAGTGATGCATTCAGCTTTTGGTCGTAAGCGATGGTCTCGGCGCAGCCGCGTTCGGCGGTCGCTCGGAAC
GACGGCCTGTTTCGGCGGAG

Downstream 100 bases:

>100_bases
GCGGCCGGGCGGCGGGCGCCGCATCGCGTCCCATCTGCACGGCGCGGCTTCGGCGGCCGCGCGTCCGTCTCAAACCGGAC
GGCGGCGCGCGCGGAATCGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MTRNTMRALWNQIATSLKTKFLVVTICVFGLVALPAYAAFEFLTRKTVVALGTLFAEKQVLFDRYRGLEALIREVSLAET
VARSPAVRAWARDEADPDKTVRGLAELEHYRLSFHDKSYFFVVHESGNYYFNDADNNYNGNQLRYQVKRDNSRDGWYFKT
IAAGQACQLNVDRDDNLAVTKVWINCVITDQGRILGVIGTGVDLSEFIREVVALPQTGITSMFVDRAGAIQAHRDPGMVD
FHSLTKDTKTKKTIFQQVDKPAEAGELAAMMARVSAGSRMVESRFMNIGGREFLVGVGYLDRLGWFNVTLMDIDAIIDRR
LFTPIALLLVFMMTAAVALVTILFRRVVLDRLAIVEAAARRVEDGDFDNIVVDRGSDEIGRLSRTLSGMAHAVRDHTRIL
EDLVDERTEKLRRLANIDLLTEIPNRRGFTQVFEQWLARSPLVPRSAGLLLIDLDDFKDVNDAFGHLAGDRLLCETARRL
APLLTDRDICARWGGDEFVVLVEDCDQDGLRALCARMVAAMNATPIRSDDGQDIALTVSIGGCRVVSGSSIDAAFAEADA
ALYAAKRAGRNRFVLFDADAGSALDAVVTAKA

Sequences:

>Translated_592_residues
MTRNTMRALWNQIATSLKTKFLVVTICVFGLVALPAYAAFEFLTRKTVVALGTLFAEKQVLFDRYRGLEALIREVSLAET
VARSPAVRAWARDEADPDKTVRGLAELEHYRLSFHDKSYFFVVHESGNYYFNDADNNYNGNQLRYQVKRDNSRDGWYFKT
IAAGQACQLNVDRDDNLAVTKVWINCVITDQGRILGVIGTGVDLSEFIREVVALPQTGITSMFVDRAGAIQAHRDPGMVD
FHSLTKDTKTKKTIFQQVDKPAEAGELAAMMARVSAGSRMVESRFMNIGGREFLVGVGYLDRLGWFNVTLMDIDAIIDRR
LFTPIALLLVFMMTAAVALVTILFRRVVLDRLAIVEAAARRVEDGDFDNIVVDRGSDEIGRLSRTLSGMAHAVRDHTRIL
EDLVDERTEKLRRLANIDLLTEIPNRRGFTQVFEQWLARSPLVPRSAGLLLIDLDDFKDVNDAFGHLAGDRLLCETARRL
APLLTDRDICARWGGDEFVVLVEDCDQDGLRALCARMVAAMNATPIRSDDGQDIALTVSIGGCRVVSGSSIDAAFAEADA
ALYAAKRAGRNRFVLFDADAGSALDAVVTAKA
>Mature_591_residues
TRNTMRALWNQIATSLKTKFLVVTICVFGLVALPAYAAFEFLTRKTVVALGTLFAEKQVLFDRYRGLEALIREVSLAETV
ARSPAVRAWARDEADPDKTVRGLAELEHYRLSFHDKSYFFVVHESGNYYFNDADNNYNGNQLRYQVKRDNSRDGWYFKTI
AAGQACQLNVDRDDNLAVTKVWINCVITDQGRILGVIGTGVDLSEFIREVVALPQTGITSMFVDRAGAIQAHRDPGMVDF
HSLTKDTKTKKTIFQQVDKPAEAGELAAMMARVSAGSRMVESRFMNIGGREFLVGVGYLDRLGWFNVTLMDIDAIIDRRL
FTPIALLLVFMMTAAVALVTILFRRVVLDRLAIVEAAARRVEDGDFDNIVVDRGSDEIGRLSRTLSGMAHAVRDHTRILE
DLVDERTEKLRRLANIDLLTEIPNRRGFTQVFEQWLARSPLVPRSAGLLLIDLDDFKDVNDAFGHLAGDRLLCETARRLA
PLLTDRDICARWGGDEFVVLVEDCDQDGLRALCARMVAAMNATPIRSDDGQDIALTVSIGGCRVVSGSSIDAAFAEADAA
LYAAKRAGRNRFVLFDADAGSALDAVVTAKA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=31.1111111111111, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787541, Length=186, Percent_Identity=34.4086021505376, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=34.8314606741573, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=34.0909090909091, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=190, Percent_Identity=33.1578947368421, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=30.952380952381, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=32.5443786982249, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=31.3953488372093, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082007, Length=129, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=30.5389221556886, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65707; Mature: 65576

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNTMRALWNQIATSLKTKFLVVTICVFGLVALPAYAAFEFLTRKTVVALGTLFAEKQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFDRYRGLEALIREVSLAETVARSPAVRAWARDEADPDKTVRGLAELEHYRLSFHDKSYF
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCEEE
FVVHESGNYYFNDADNNYNGNQLRYQVKRDNSRDGWYFKTIAAGQACQLNVDRDDNLAVT
EEEECCCCEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCEEEE
KVWINCVITDQGRILGVIGTGVDLSEFIREVVALPQTGITSMFVDRAGAIQAHRDPGMVD
EEEEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCCEE
FHSLTKDTKTKKTIFQQVDKPAEAGELAAMMARVSAGSRMVESRFMNIGGREFLVGVGYL
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEEECHHH
DRLGWFNVTLMDIDAIIDRRLFTPIALLLVFMMTAAVALVTILFRRVVLDRLAIVEAAAR
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVEDGDFDNIVVDRGSDEIGRLSRTLSGMAHAVRDHTRILEDLVDERTEKLRRLANIDLL
HCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TEIPNRRGFTQVFEQWLARSPLVPRSAGLLLIDLDDFKDVNDAFGHLAGDRLLCETARRL
HHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHH
APLLTDRDICARWGGDEFVVLVEDCDQDGLRALCARMVAAMNATPIRSDDGQDIALTVSI
HHHHCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
GGCRVVSGSSIDAAFAEADAALYAAKRAGRNRFVLFDADAGSALDAVVTAKA
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEECCC
>Mature Secondary Structure 
TRNTMRALWNQIATSLKTKFLVVTICVFGLVALPAYAAFEFLTRKTVVALGTLFAEKQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFDRYRGLEALIREVSLAETVARSPAVRAWARDEADPDKTVRGLAELEHYRLSFHDKSYF
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCEEE
FVVHESGNYYFNDADNNYNGNQLRYQVKRDNSRDGWYFKTIAAGQACQLNVDRDDNLAVT
EEEECCCCEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCEEEE
KVWINCVITDQGRILGVIGTGVDLSEFIREVVALPQTGITSMFVDRAGAIQAHRDPGMVD
EEEEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCCEE
FHSLTKDTKTKKTIFQQVDKPAEAGELAAMMARVSAGSRMVESRFMNIGGREFLVGVGYL
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEEECHHH
DRLGWFNVTLMDIDAIIDRRLFTPIALLLVFMMTAAVALVTILFRRVVLDRLAIVEAAAR
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVEDGDFDNIVVDRGSDEIGRLSRTLSGMAHAVRDHTRILEDLVDERTEKLRRLANIDLL
HCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TEIPNRRGFTQVFEQWLARSPLVPRSAGLLLIDLDDFKDVNDAFGHLAGDRLLCETARRL
HHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHH
APLLTDRDICARWGGDEFVVLVEDCDQDGLRALCARMVAAMNATPIRSDDGQDIALTVSI
HHHHCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
GGCRVVSGSSIDAAFAEADAALYAAKRAGRNRFVLFDADAGSALDAVVTAKA
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]