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The map label for this gene is pleD [H]

Identifier: 86751216

GI number: 86751216

Start: 4678020

End: 4678982

Strand: Reverse

Name: pleD [H]

Synonym: RPB_4108

Alternate gene names: 86751216

Gene position: 4678982-4678020 (Counterclockwise)

Preceding gene: 86751217

Following gene: 86751215

Centisome position: 87.76

GC content: 62.72

Gene sequence:

>963_bases
ATGCGAATTCTGGTTATCGACGACAGTGAAGACGGACGTGATGTGGCGGAAGCCATGTTGATTGCCGCAGGTTACGAGGA
CGTCCAAACATCCGATTCGGCGGCGGATGCGTATCAGATACTCGCGGTGGGCCATTCAACTGCGGCCAAAGTGTCGCCGG
TAGATCTGATCCTGATGGACATCATGATGCCCGAGATCGACGGCATCGAAGCTTGCGCCCGCATTCGCGGCGAACCGCAC
CTGATGGATGTGCCGATCATCATGGTGACGTTGCTCGACGACGTCGACAGTCTCGCCAATGCGTTCGTGGCCGGCGCTAC
GGACTACATCACCAAGCCGCTCAAACGGGTCGAGCTGCACGCTCGGGTTCGTTCGGCGCTGAAGTTGAAGTCCGAACTCG
ATCGACGCCGCGCGCGGGAGCGGGAACTGTTGGAGTTCTTGGCGACCTTGGGCGACCGTCGGACGTCACACTGGATCGAC
GAAGGAACCGGACTGATCATCGGGGAGGTGGCCGAAGCCTACCTGACCGCTGAAAATCAGGGCGGCGGCGACCTGTCCGT
CATTGCTCTCGGTCTCGATCGGCTGGAAGCCTTGCGCCAGATCCTGGGCGATGCGGCAGCGGCGAACATCGTGTCCCGCG
TGGCCCGCTTGGTCAGAGCGGCGACAGCGACGGTCGGAGTCGTCGCCGGCGCTTATCGCAATGGCACCATCGTTCTGATC
GTTCCGGAATTGCCCCGGCAGCGCGCACTCGATCTCGGCGAAGCGCTGCGTGCCGCTGTGTCCGATCTTCGTATTGCGGA
ACCGGAAGCGACTTGCGCCGACCACGCGACCGCGAGCGTCGCGGTCGTCACCGGACGAGTGGATAGCAGCGTCGAGAGAA
TTCACTTGCTGACACGCGCGATGGCGGCGGTGTCCCGCGCGGCTGCCGAAGGTGGCGATCGCATTGTGAGTGAAGCCGCC
TGA

Upstream 100 bases:

>100_bases
CCAAAACGCGAACTGGAGACTGTTCGCGCGGGACTGATGCTGCCTGGAATCGCCGTCGCTCCGGATTGAGCCGTCATTCG
ACAAACAGGAGACTATCTCC

Downstream 100 bases:

>100_bases
CGTTGTTGCGGTCACGAAACGCCTTGCAGGGGAGTGCTGAGTGGTGCGCGGACGGGTGGGGTTGGCAGTGGTGATTGCAA
CTGGTGTGACGGCGTCTCAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRILVIDDSEDGRDVAEAMLIAAGYEDVQTSDSAADAYQILAVGHSTAAKVSPVDLILMDIMMPEIDGIEACARIRGEPH
LMDVPIIMVTLLDDVDSLANAFVAGATDYITKPLKRVELHARVRSALKLKSELDRRRARERELLEFLATLGDRRTSHWID
EGTGLIIGEVAEAYLTAENQGGGDLSVIALGLDRLEALRQILGDAAAANIVSRVARLVRAATATVGVVAGAYRNGTIVLI
VPELPRQRALDLGEALRAAVSDLRIAEPEATCADHATASVAVVTGRVDSSVERIHLLTRAMAAVSRAAAEGGDRIVSEAA

Sequences:

>Translated_320_residues
MRILVIDDSEDGRDVAEAMLIAAGYEDVQTSDSAADAYQILAVGHSTAAKVSPVDLILMDIMMPEIDGIEACARIRGEPH
LMDVPIIMVTLLDDVDSLANAFVAGATDYITKPLKRVELHARVRSALKLKSELDRRRARERELLEFLATLGDRRTSHWID
EGTGLIIGEVAEAYLTAENQGGGDLSVIALGLDRLEALRQILGDAAAANIVSRVARLVRAATATVGVVAGAYRNGTIVLI
VPELPRQRALDLGEALRAAVSDLRIAEPEATCADHATASVAVVTGRVDSSVERIHLLTRAMAAVSRAAAEGGDRIVSEAA
>Mature_320_residues
MRILVIDDSEDGRDVAEAMLIAAGYEDVQTSDSAADAYQILAVGHSTAAKVSPVDLILMDIMMPEIDGIEACARIRGEPH
LMDVPIIMVTLLDDVDSLANAFVAGATDYITKPLKRVELHARVRSALKLKSELDRRRARERELLEFLATLGDRRTSHWID
EGTGLIIGEVAEAYLTAENQGGGDLSVIALGLDRLEALRQILGDAAAANIVSRVARLVRAATATVGVVAGAYRNGTIVLI
VPELPRQRALDLGEALRAAVSDLRIAEPEATCADHATASVAVVTGRVDSSVERIHLLTRAMAAVSRAAAEGGDRIVSEAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=33.0769230769231, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=122, Percent_Identity=36.0655737704918, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=127, Percent_Identity=29.9212598425197, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34057; Mature: 34057

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIDDSEDGRDVAEAMLIAAGYEDVQTSDSAADAYQILAVGHSTAAKVSPVDLILMD
CEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHEEECCCCCCCCCHHHHHHHH
IMMPEIDGIEACARIRGEPHLMDVPIIMVTLLDDVDSLANAFVAGATDYITKPLKRVELH
HHCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARVRSALKLKSELDRRRARERELLEFLATLGDRRTSHWIDEGTGLIIGEVAEAYLTAENQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEHHHHHHHHHCCCCC
GGGDLSVIALGLDRLEALRQILGDAAAANIVSRVARLVRAATATVGVVAGAYRNGTIVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VPELPRQRALDLGEALRAAVSDLRIAEPEATCADHATASVAVVTGRVDSSVERIHLLTRA
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
MAAVSRAAAEGGDRIVSEAA
HHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MRILVIDDSEDGRDVAEAMLIAAGYEDVQTSDSAADAYQILAVGHSTAAKVSPVDLILMD
CEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHEEECCCCCCCCCHHHHHHHH
IMMPEIDGIEACARIRGEPHLMDVPIIMVTLLDDVDSLANAFVAGATDYITKPLKRVELH
HHCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARVRSALKLKSELDRRRARERELLEFLATLGDRRTSHWIDEGTGLIIGEVAEAYLTAENQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEHHHHHHHHHCCCCC
GGGDLSVIALGLDRLEALRQILGDAAAANIVSRVARLVRAATATVGVVAGAYRNGTIVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VPELPRQRALDLGEALRAAVSDLRIAEPEATCADHATASVAVVTGRVDSSVERIHLLTRA
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
MAAVSRAAAEGGDRIVSEAA
HHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA