Click here to switch to the map view.

The map label for this gene is ilvD

Identifier: 86751174

GI number: 86751174

Start: 4638581

End: 4640440

Strand: Reverse

Name: ilvD

Synonym: RPB_4066

Alternate gene names: 86751174

Gene position: 4640440-4638581 (Counterclockwise)

Preceding gene: 86751176

Following gene: 86751172

Centisome position: 87.04

GC content: 66.94

Gene sequence:

>1860_bases
ATGCCTGCATATCGATCCCGAACGACCACTCACGGCCGCAACATGGCCGGCGCGCGCGGCCTCTGGCGCGCCACCGGGAT
GAAGGATTCCGACTTCGGCAAGCCGATCATCGCCGTCGTCAACTCCTTCACGCAGTTCGTGCCGGGGCACGTGCATCTGA
AGGATCTCGGCCAGCTCGTCGCCCGGGAGATCGAGGCCGCCGGCGGCGTCGCCAAGGAATTCAACACCATCGCGGTCGAC
GACGGCATCGCGATGGGCCATGACGGCATGCTGTACAGCCTGCCGTCGCGCGAACTGATCGCCGACAGCGTCGAATACAT
GGTCAACGCGCACTGCGCCGACGCGATGGTCTGCATCTCGAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCGA
TGCGGCTCAACATCCCAGCGGTGTTCGTCTCCGGCGGGCCGATGGAAGCGGGCAAAGTGGTGTTGAAGGGCAAGACCCAC
GCCGTCGACCTGATCGACGCGATGGTCGCGGCCGCCGACAGCAGCATGAGCGACGAAGACGTGCAGACGATGGAGCGCTC
GGCGTGCCCGACCTGCGGCTCCTGCTCCGGCATGTTCACCGCCAATTCGATGAACTGTCTCGCCGAGGCGCTGGGTCTGG
CGCTGCCCGGCAACGGCTCGGTGCTCGCCACCCATGCCGATCGCAAGCGGCTGTTCGTCGAGGCCGGTCACACCATCGTC
GATCTGGCGCGGCGCTACTACGAAGGCGACGACGAATCCGTGCTGCCGCGCAAGGTCGCGAGCTTCGAGGCGTTCGAGAA
CGCGATGACGCTCGACATCGCGATGGGCGGCTCGACCAACACGGTGCTGCATCTGCTCGCCGCGGCGCGCGAGGCCGAAC
TCGACTTCTCGATGAAGGACATCGACCGGCTGTCGCGCAAGGTGCCGTGCCTGAGCAAGATCGCCCCGTCGGTGTCCGAC
GTCCACATGGAGGACGTGCATCGCGCCGGCGGCATCATGGCGATCCTCGGCGAGCTCGATCGCGCCGGACTGATCCACAA
CTCATGCCCGACTGTGCATTCGGAGACGCTCGGTGCCGCGCTGGCGCGCTGGGACATCCGCCAGAGCAACAGCGAAGCGG
TCCGCACCTTCTACCGCGCCGCGCCGGGCGGCGTGCCGACCCAGGTTGCGTTCAGCCAGGACCGCCGCTACGACGAGCTC
GACCTCGACCGGCAGAAGGGCGTGATCCGCGACGCCGAGCACGCCTTCAGCAAGGACGGCGGCCTCGCGGTGCTGTACGG
CAATATTGCGCTCGACGGCTGCATCGTGAAGACCGCCGGCGTCGACGCCTCGATCCTGACCTTCTCCGGCCCGGCGAAAG
TGTTCGAGAGCCAGGACGACGCGGTGTCGGCGATCCTCGGCAACAAGATCGTCGCTGGCGACGTCATCGTGATCCGCTAC
GAAGGGCCACGTGGCGGGCCGGGCATGCAGGAGATGCTGTATCCGACCAGCTATCTGAAGTCGAAGGGCCTCGGCAAAGC
CTGCGCGCTGATCACCGACGGCCGCTTCTCCGGCGGCACCTCGGGCCTGTCGATCGGTCACGTCTCGCCCGAGGCTGCGG
AAGGCGGCCTGATCGGACTGGTGCGGAACGGCGACCGGATTTCGATCGACATTCCCAATCGCGGCATCACCCTTGACGTC
GCCGCTGACGAGCTGTCGCGGCGCGCCGAGGAGGAAGAGGCGAAGGGCGACAAGGCCTGGCAGCCGAAAGACCGCAAGCG
CAAGGTCTCGGCCGCGCTGCAGGCCTATGCCATGCTGACCACCAGCGCTGCGAACGGCGCGGTGCGCGACGTCAACCGCA
GGCTCGGCAAAGGAAAGTAG

Upstream 100 bases:

>100_bases
TGTGGCCAGACGACGATGTAACGGTTCGTCGCGTCGGGCGTTGAATGCGGTTCTGGCTGCGCGCCCGCCCACGCAACGGA
CAATTCTCACGGACACAGTC

Downstream 100 bases:

>100_bases
CAAGCACCTCGAGCGGCCGTGACTCTCGGCCCGCCGACGCCGACCATACAGAACGAGCGCCGCGATGGCGCTCGTTTCGT
TTTGAGCAGAGCCGCAGGTT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVD
DGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVVLKGKTH
AVDLIDAMVAAADSSMSDEDVQTMERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGSVLATHADRKRLFVEAGHTIV
DLARRYYEGDDESVLPRKVASFEAFENAMTLDIAMGGSTNTVLHLLAAAREAELDFSMKDIDRLSRKVPCLSKIAPSVSD
VHMEDVHRAGGIMAILGELDRAGLIHNSCPTVHSETLGAALARWDIRQSNSEAVRTFYRAAPGGVPTQVAFSQDRRYDEL
DLDRQKGVIRDAEHAFSKDGGLAVLYGNIALDGCIVKTAGVDASILTFSGPAKVFESQDDAVSAILGNKIVAGDVIVIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGLIGLVRNGDRISIDIPNRGITLDV
AADELSRRAEEEEAKGDKAWQPKDRKRKVSAALQAYAMLTTSAANGAVRDVNRRLGKGK

Sequences:

>Translated_619_residues
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVD
DGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVVLKGKTH
AVDLIDAMVAAADSSMSDEDVQTMERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGSVLATHADRKRLFVEAGHTIV
DLARRYYEGDDESVLPRKVASFEAFENAMTLDIAMGGSTNTVLHLLAAAREAELDFSMKDIDRLSRKVPCLSKIAPSVSD
VHMEDVHRAGGIMAILGELDRAGLIHNSCPTVHSETLGAALARWDIRQSNSEAVRTFYRAAPGGVPTQVAFSQDRRYDEL
DLDRQKGVIRDAEHAFSKDGGLAVLYGNIALDGCIVKTAGVDASILTFSGPAKVFESQDDAVSAILGNKIVAGDVIVIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGLIGLVRNGDRISIDIPNRGITLDV
AADELSRRAEEEEAKGDKAWQPKDRKRKVSAALQAYAMLTTSAANGAVRDVNRRLGKGK
>Mature_618_residues
PAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDD
GIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVVLKGKTHA
VDLIDAMVAAADSSMSDEDVQTMERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGSVLATHADRKRLFVEAGHTIVD
LARRYYEGDDESVLPRKVASFEAFENAMTLDIAMGGSTNTVLHLLAAAREAELDFSMKDIDRLSRKVPCLSKIAPSVSDV
HMEDVHRAGGIMAILGELDRAGLIHNSCPTVHSETLGAALARWDIRQSNSEAVRTFYRAAPGGVPTQVAFSQDRRYDELD
LDRQKGVIRDAEHAFSKDGGLAVLYGNIALDGCIVKTAGVDASILTFSGPAKVFESQDDAVSAILGNKIVAGDVIVIRYE
GPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGLIGLVRNGDRISIDIPNRGITLDVA
ADELSRRAEEEEAKGDKAWQPKDRKRKVSAALQAYAMLTTSAANGAVRDVNRRLGKGK

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=613, Percent_Identity=74.0619902120718, Blast_Score=943, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=543, Percent_Identity=30.0184162062615, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI2367371, Length=572, Percent_Identity=30.4195804195804, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786464, Length=522, Percent_Identity=32.9501915708812, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322476, Length=613, Percent_Identity=36.2153344208809, Blast_Score=344, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_RHOP2 (Q2ISQ1)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_487670.1
- STRING:   Q2ISQ1
- GeneID:   3911873
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_4066
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- ProtClustDB:   PRK12448
- BioCyc:   RPAL316058:RPB_4066-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 65788; Mature: 65657

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVVLKGKTHAVDLIDAMVAAADSSMSDED
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHH
VQTMERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGSVLATHADRKRLFVEAGHTIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEECCCCCCEEEEECCCCEEEEECCCHHH
DLARRYYEGDDESVLPRKVASFEAFENAMTLDIAMGGSTNTVLHLLAAAREAELDFSMKD
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHH
IDRLSRKVPCLSKIAPSVSDVHMEDVHRAGGIMAILGELDRAGLIHNSCPTVHSETLGAA
HHHHHHCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LARWDIRQSNSEAVRTFYRAAPGGVPTQVAFSQDRRYDELDLDRQKGVIRDAEHAFSKDG
HHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHCCCC
GLAVLYGNIALDGCIVKTAGVDASILTFSGPAKVFESQDDAVSAILGNKIVAGDVIVIRY
CEEEEECCEEECCEEEEECCCCEEEEEECCCHHHHCCCCHHHHHHHCCEEEECCEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGLIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
VRNGDRISIDIPNRGITLDVAADELSRRAEEEEAKGDKAWQPKDRKRKVSAALQAYAMLT
EECCCEEEEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TSAANGAVRDVNRRLGKGK
HCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
PAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVVLKGKTHAVDLIDAMVAAADSSMSDED
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHH
VQTMERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGSVLATHADRKRLFVEAGHTIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEECCCCCCEEEEECCCCEEEEECCCHHH
DLARRYYEGDDESVLPRKVASFEAFENAMTLDIAMGGSTNTVLHLLAAAREAELDFSMKD
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHH
IDRLSRKVPCLSKIAPSVSDVHMEDVHRAGGIMAILGELDRAGLIHNSCPTVHSETLGAA
HHHHHHCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LARWDIRQSNSEAVRTFYRAAPGGVPTQVAFSQDRRYDELDLDRQKGVIRDAEHAFSKDG
HHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHCCCC
GLAVLYGNIALDGCIVKTAGVDASILTFSGPAKVFESQDDAVSAILGNKIVAGDVIVIRY
CEEEEECCEEECCEEEEECCCCEEEEEECCCHHHHCCCCHHHHHHHCCEEEECCEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGLIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
VRNGDRISIDIPNRGITLDVAADELSRRAEEEEAKGDKAWQPKDRKRKVSAALQAYAMLT
EECCCEEEEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TSAANGAVRDVNRRLGKGK
HCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA