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The map label for this gene is gatA [H]

Identifier: 86751162

GI number: 86751162

Start: 4624766

End: 4626169

Strand: Direct

Name: gatA [H]

Synonym: RPB_4054

Alternate gene names: 86751162

Gene position: 4624766-4626169 (Clockwise)

Preceding gene: 86751161

Following gene: 86751163

Centisome position: 86.74

GC content: 69.73

Gene sequence:

>1404_bases
ATGAAGTCCGAAACATCCTCCGGCTGGATGACCGCCGCCGAGATCGCGGCGGCCGTCGCCAACCGGACGATGACCGCCCT
CGACGCCACCGAAGCGGCGCTGGCGCGGATCGCGCAGCGCGACGCGACCCTGAATGCCTTCACCGACATCGTCGCCGAGC
GCGCCCGCAATCGCGCCCGCGCGATCGATGCAGCGATCGCGCGCGGCGAACAGGTCGGCCCGCTCGCCGGCGTGCCGTTC
GCGGTGAAGAACCTGTTCAATGTCGCGGGCCTGACGACCCGCGCCGGCTCCAAGATCAATCGCGACCTTGCCCCCGCGAA
ACGCGATGCCACGCTGATCGAGCGGCTGGAAGCCGCCGGCGCGGTGCTGATCGGCGCGCTCAACATGGGCGAATACGCCT
ACGACTTCACCGGCGAGAATTTTCACGACGGCCCGTCGCGCAATCCGCACGACCCGACGCGGATGACCGGCGGCTCGTCC
GGCGGTTCGGGTGCCGCGGTCGGCGGCGGCGAGGTGCCGCTGGCGCTCGGCTCGGATACCAATGGCTCGATCCGGGTGCC
GTCGTCGTTTTGCGGCATCTTCGGGCTGAAGCCGACCTATGGCCGGCTGCCGCGCTCGCGCTCGTTTCCGTTCGTCGCGA
GCTTCGATCACCTCGGCCCGTTCGCGCGCAACGTCGCAGATCTCGCGCTCGCCTATGACGTGATGCAGGGGCCGGATTCC
GACGACGCCGCCTGCTCGACGCGTTCGATCGAGCCGGTTCACGCCGCGCTGGCGCAAGGCCTCGACGGCCTGCGCATCGC
GCGGGCCGGTGGGTACTTCGCGGCCAATCTGTTTCCCGAAGCCCGTGAAGCCGTCGATCGCGTGGCGAAGGCGCTCAGTA
TCACCATCACCGTGGAACTGCCCGAAGCCGCGCGCGCCCGCGCCGCGGCCTTCGTCATCACCACTGTCGAGGGCGCGTCG
CTGCATCTCGATCGTCTGCGCCAGCGGCCGAATGATTTCGACCCCGCGGTGCGCGACCGGCTGATTGCAGGGGCGATGAT
CCCGGCGCCGCTGGTCGACCGCGCGCAGAAATTTCGCCGCTGGTATCGCGCGCGCGTGCTCGAGTTGTTCAGGGACGTGG
ATGTCATCATCGCGCCGGCGACCCCCTGCGTCGCGCCGAAGCTCGGGCAGCAGAGCTTCATGCTCGACGGCGTGGAGCTG
CCGGTGCGCGCCAATATCGGCATCCACACCCAGCCGATCTCGTTCATCGGCCTCCCGGTCGTGGCCGTGCCGATCCCGCT
CGAGCCGATGCCGATCGGCATCCAGATCATCGCCGCGCCGTGGCGCGAGGACCTCGCGCTGCGCGTCGCGCATGCGCTCG
AAACAGCCGGCGTCGCCTGCGCGCCGCGCCCGCAAACCTTTTGA

Upstream 100 bases:

>100_bases
GTGCGCGCCAATCTCGAAGTGTCACTGAGGCTGGCGCGGCAGGTCGACGAGTGTCCGCTTCCCGACGAGACCGAACCCGC
GAGTGTCTTCAGTGCCTGAG

Downstream 100 bases:

>100_bases
TTGGATTCTGACATGGAACTCGACCTGCCCGACGTGCTCGCAGAAGTCGGCGACGCTTTCGCCCGCTACGAGGCGGCGCT
GATCAGCAACGACGTCGCCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKSETSSGWMTAAEIAAAVANRTMTALDATEAALARIAQRDATLNAFTDIVAERARNRARAIDAAIARGEQVGPLAGVPF
AVKNLFNVAGLTTRAGSKINRDLAPAKRDATLIERLEAAGAVLIGALNMGEYAYDFTGENFHDGPSRNPHDPTRMTGGSS
GGSGAAVGGGEVPLALGSDTNGSIRVPSSFCGIFGLKPTYGRLPRSRSFPFVASFDHLGPFARNVADLALAYDVMQGPDS
DDAACSTRSIEPVHAALAQGLDGLRIARAGGYFAANLFPEAREAVDRVAKALSITITVELPEAARARAAAFVITTVEGAS
LHLDRLRQRPNDFDPAVRDRLIAGAMIPAPLVDRAQKFRRWYRARVLELFRDVDVIIAPATPCVAPKLGQQSFMLDGVEL
PVRANIGIHTQPISFIGLPVVAVPIPLEPMPIGIQIIAAPWREDLALRVAHALETAGVACAPRPQTF

Sequences:

>Translated_467_residues
MKSETSSGWMTAAEIAAAVANRTMTALDATEAALARIAQRDATLNAFTDIVAERARNRARAIDAAIARGEQVGPLAGVPF
AVKNLFNVAGLTTRAGSKINRDLAPAKRDATLIERLEAAGAVLIGALNMGEYAYDFTGENFHDGPSRNPHDPTRMTGGSS
GGSGAAVGGGEVPLALGSDTNGSIRVPSSFCGIFGLKPTYGRLPRSRSFPFVASFDHLGPFARNVADLALAYDVMQGPDS
DDAACSTRSIEPVHAALAQGLDGLRIARAGGYFAANLFPEAREAVDRVAKALSITITVELPEAARARAAAFVITTVEGAS
LHLDRLRQRPNDFDPAVRDRLIAGAMIPAPLVDRAQKFRRWYRARVLELFRDVDVIIAPATPCVAPKLGQQSFMLDGVEL
PVRANIGIHTQPISFIGLPVVAVPIPLEPMPIGIQIIAAPWREDLALRVAHALETAGVACAPRPQTF
>Mature_467_residues
MKSETSSGWMTAAEIAAAVANRTMTALDATEAALARIAQRDATLNAFTDIVAERARNRARAIDAAIARGEQVGPLAGVPF
AVKNLFNVAGLTTRAGSKINRDLAPAKRDATLIERLEAAGAVLIGALNMGEYAYDFTGENFHDGPSRNPHDPTRMTGGSS
GGSGAAVGGGEVPLALGSDTNGSIRVPSSFCGIFGLKPTYGRLPRSRSFPFVASFDHLGPFARNVADLALAYDVMQGPDS
DDAACSTRSIEPVHAALAQGLDGLRIARAGGYFAANLFPEAREAVDRVAKALSITITVELPEAARARAAAFVITTVEGAS
LHLDRLRQRPNDFDPAVRDRLIAGAMIPAPLVDRAQKFRRWYRARVLELFRDVDVIIAPATPCVAPKLGQQSFMLDGVEL
PVRANIGIHTQPISFIGLPVVAVPIPLEPMPIGIQIIAAPWREDLALRVAHALETAGVACAPRPQTF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=27.4747474747475, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=28.5429141716567, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=504, Percent_Identity=24.6031746031746, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=414, Percent_Identity=28.9855072463768, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=27.1255060728745, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=227, Percent_Identity=30.8370044052863, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=503, Percent_Identity=23.8568588469185, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=457, Percent_Identity=23.8512035010941, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=190, Percent_Identity=31.0526315789474, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=190, Percent_Identity=31.0526315789474, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=194, Percent_Identity=26.2886597938144, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=389, Percent_Identity=28.7917737789203, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=380, Percent_Identity=24.2105263157895, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=28.9205702647658, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=25.9713701431493, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24652985, Length=487, Percent_Identity=28.5420944558522, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=487, Percent_Identity=28.5420944558522, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=487, Percent_Identity=28.5420944558522, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=487, Percent_Identity=28.5420944558522, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45550774, Length=486, Percent_Identity=26.1316872427983, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648435, Length=486, Percent_Identity=26.1316872427983, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648437, Length=486, Percent_Identity=26.1316872427983, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078093, Length=494, Percent_Identity=25.9109311740891, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=26.8292682926829, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=25.5868544600939, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=25.5868544600939, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49463; Mature: 49463

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSETSSGWMTAAEIAAAVANRTMTALDATEAALARIAQRDATLNAFTDIVAERARNRAR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIDAAIARGEQVGPLAGVPFAVKNLFNVAGLTTRAGSKINRDLAPAKRDATLIERLEAAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCHHCCCCCCCHHHHHHHHHHHHCC
AVLIGALNMGEYAYDFTGENFHDGPSRNPHDPTRMTGGSSGGSGAAVGGGEVPLALGSDT
EEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
NGSIRVPSSFCGIFGLKPTYGRLPRSRSFPFVASFDHLGPFARNVADLALAYDVMQGPDS
CCCEECCHHHHHHCCCCCCHHCCCCCCCCCEEECCHHCCHHHHHHHHHHHHHHHHCCCCC
DDAACSTRSIEPVHAALAQGLDGLRIARAGGYFAANLFPEAREAVDRVAKALSITITVEL
CCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHEEEEEEEEC
PEAARARAAAFVITTVEGASLHLDRLRQRPNDFDPAVRDRLIAGAMIPAPLVDRAQKFRR
CHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
WYRARVLELFRDVDVIIAPATPCVAPKLGQQSFMLDGVELPVRANIGIHTQPISFIGLPV
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCCCCEECCCCCEECCHHHHCCCE
VAVPIPLEPMPIGIQIIAAPWREDLALRVAHALETAGVACAPRPQTF
EEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure
MKSETSSGWMTAAEIAAAVANRTMTALDATEAALARIAQRDATLNAFTDIVAERARNRAR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIDAAIARGEQVGPLAGVPFAVKNLFNVAGLTTRAGSKINRDLAPAKRDATLIERLEAAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCHHCCCCCCCHHHHHHHHHHHHCC
AVLIGALNMGEYAYDFTGENFHDGPSRNPHDPTRMTGGSSGGSGAAVGGGEVPLALGSDT
EEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
NGSIRVPSSFCGIFGLKPTYGRLPRSRSFPFVASFDHLGPFARNVADLALAYDVMQGPDS
CCCEECCHHHHHHCCCCCCHHCCCCCCCCCEEECCHHCCHHHHHHHHHHHHHHHHCCCCC
DDAACSTRSIEPVHAALAQGLDGLRIARAGGYFAANLFPEAREAVDRVAKALSITITVEL
CCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHEEEEEEEEC
PEAARARAAAFVITTVEGASLHLDRLRQRPNDFDPAVRDRLIAGAMIPAPLVDRAQKFRR
CHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
WYRARVLELFRDVDVIIAPATPCVAPKLGQQSFMLDGVELPVRANIGIHTQPISFIGLPV
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCCCCEECCCCCEECCHHHHCCCE
VAVPIPLEPMPIGIQIIAAPWREDLALRVAHALETAGVACAPRPQTF
EEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA