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The map label for this gene is cysU [H]

Identifier: 86751153

GI number: 86751153

Start: 4615052

End: 4615867

Strand: Reverse

Name: cysU [H]

Synonym: RPB_4045

Alternate gene names: 86751153

Gene position: 4615867-4615052 (Counterclockwise)

Preceding gene: 86751154

Following gene: 86751152

Centisome position: 86.57

GC content: 68.38

Gene sequence:

>816_bases
ATGTCACAAAGATCGTTCTCACTCGCCTGCCTGACGCCGCTTGTGGTGCTGACGGCGGCATTCTTCGTTTTGCCGATGGC
TCGGCTCGTGGTCGTCGGCGCCGGGGGGCCGCAGGGCGTCGCCGGCTATCTCGCCATCTTGCTCGAGCCGCGCTACCGCG
CCACGCTGATCAATACCGTATTGCTGGCGAGCGCGACCACGCTCGCGACGCTGGTGATCGCCGCCATCGCGGCGATCTTC
CTGCAGCGCCATCGTTTCCCCGGGCGCGCGGTCCTGGTCGCGATGCTGACCTTTCCGCTGGCGTTTCCGGGCGTGGTGAT
CGGCTTCCTGATCATCCTGCTCGCCGGGCGGCAGGGGCTGATCGGCGACGTCACGCGGCTGCTCGGCGGCGGCAAGGTGG
TGTTCGCCTATTCGATCTGGGGGCTGTTCCTGGGCTATCTGTATTTCTCGATTCCGCGCGTCATCCTCACCATCATGGCG
GCGGTGCAGAAGCTCGATCCCGGCCTCGAGGAGGCCGCCCGCTCGCTCGGTGCGAGCCCTTGGGCGGTGCAACGCGACGT
CGTGCTGCCGGCGCTCGGACCGGCCTTCGTCGCCTCGGGCGCGATCGCGTTCGCGACCGCGATGGGGGCGTTCGGCACCG
CCTTCACGCTGGCCACCAATATCGACGTGTTGCCGATGCTGATCTACACCGAGTTCACGCTGGCGGCGAACTTCGCCACC
GCCGCGTCGCTGTCGGTCGGTCTCGGGCTGATCTCCTGGGCGATGCTGGCGCTGGCGCGCTCGCTCTCGGGCGGCACGGT
CGCGGCGACCGGTTAG

Upstream 100 bases:

>100_bases
TGATCGCTATCTTGCTGAGGTTCGCTGAGCGCCGGTTCGCTCGAACGTTCAACAATGCCATGATGCATCCGGCCGCAGCG
CGGCCGGGTGCGGACCCGTG

Downstream 100 bases:

>100_bases
CATCATGCGCGACCGCCTGATCTTCGCCGGACAATTGCTGTTCACGCTACTGGTCGCGGCGTTTCTCGTCGTGCCGGCCC
TGCTGTCGATCATGGCCGGC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSQRSFSLACLTPLVVLTAAFFVLPMARLVVVGAGGPQGVAGYLAILLEPRYRATLINTVLLASATTLATLVIAAIAAIF
LQRHRFPGRAVLVAMLTFPLAFPGVVIGFLIILLAGRQGLIGDVTRLLGGGKVVFAYSIWGLFLGYLYFSIPRVILTIMA
AVQKLDPGLEEAARSLGASPWAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAANFAT
AASLSVGLGLISWAMLALARSLSGGTVAATG

Sequences:

>Translated_271_residues
MSQRSFSLACLTPLVVLTAAFFVLPMARLVVVGAGGPQGVAGYLAILLEPRYRATLINTVLLASATTLATLVIAAIAAIF
LQRHRFPGRAVLVAMLTFPLAFPGVVIGFLIILLAGRQGLIGDVTRLLGGGKVVFAYSIWGLFLGYLYFSIPRVILTIMA
AVQKLDPGLEEAARSLGASPWAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAANFAT
AASLSVGLGLISWAMLALARSLSGGTVAATG
>Mature_270_residues
SQRSFSLACLTPLVVLTAAFFVLPMARLVVVGAGGPQGVAGYLAILLEPRYRATLINTVLLASATTLATLVIAAIAAIFL
QRHRFPGRAVLVAMLTFPLAFPGVVIGFLIILLAGRQGLIGDVTRLLGGGKVVFAYSIWGLFLGYLYFSIPRVILTIMAA
VQKLDPGLEEAARSLGASPWAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAANFATA
ASLSVGLGLISWAMLALARSLSGGTVAATG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=209, Percent_Identity=32.5358851674641, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786980, Length=214, Percent_Identity=34.1121495327103, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787368, Length=167, Percent_Identity=27.5449101796407, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28072; Mature: 27941

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRSFSLACLTPLVVLTAAFFVLPMARLVVVGAGGPQGVAGYLAILLEPRYRATLINTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHH
LLASATTLATLVIAAIAAIFLQRHRFPGRAVLVAMLTFPLAFPGVVIGFLIILLAGRQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IGDVTRLLGGGKVVFAYSIWGLFLGYLYFSIPRVILTIMAAVQKLDPGLEEAARSLGASP
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
WAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAANFAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AASLSVGLGLISWAMLALARSLSGGTVAATG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
SQRSFSLACLTPLVVLTAAFFVLPMARLVVVGAGGPQGVAGYLAILLEPRYRATLINTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHH
LLASATTLATLVIAAIAAIFLQRHRFPGRAVLVAMLTFPLAFPGVVIGFLIILLAGRQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IGDVTRLLGGGKVVFAYSIWGLFLGYLYFSIPRVILTIMAAVQKLDPGLEEAARSLGASP
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
WAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAANFAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AASLSVGLGLISWAMLALARSLSGGTVAATG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]