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The map label for this gene is ltaA [H]

Identifier: 86750810

GI number: 86750810

Start: 4238464

End: 4239531

Strand: Direct

Name: ltaA [H]

Synonym: RPB_3700

Alternate gene names: 86750810

Gene position: 4238464-4239531 (Clockwise)

Preceding gene: 86750809

Following gene: 86750815

Centisome position: 79.5

GC content: 71.44

Gene sequence:

>1068_bases
ATGGCCTACACCCCTGCCCCGCCCGATCCGAACGCGCCGCCCGTCCGGATCAATCTTCTCTCCGACACCCAGACGCGCCC
CACGCCGGCGATGCGCGAGGCGATGGCGCGGGCCGCGGTCGGCGACGAGCAGACCGGCGACGATCCGACCGTCAACGCGC
TGAACGAACGGGTCGCCGCACTGCTCGGCAAGGAAGCCGCGGTGTTCCTGCCGTCCGGCACGATGTGCAACGTCACCGCG
ACGCTCGCCACCTGCCGCCCCGGCGACGAGATCATCGCGCATCGCACCGCGCACATCCTGTCGCGCGAGGGCGGCGCGCA
TGCGGCGCTCGGCGGTTTCCAGATCACCGGCCTCGACGGCGACGACGGACAGTTCTCGCTCGATGCGTTCCGCGCCGCAC
TGCATCCGCGCTCGCGCTACGAGCCGCCGCAGACCATGGTCAGCGTCGAGCAGACCGCCAATATCGGCGGCGGCACGATC
TGGCCGCAGGCCACGCTCGACGCCATCGCGGCGGCCGGCAAGGAAGCCGGGCTCGCCACCCATATGGACGGCGCAAGGCT
GATGAACGCGGCGGTCGCCACCAGCATCGCGGCGCACGAGATGGCGGCGGGCTGGGATTCGGCGTGGATCGATTTCAGCA
AGGGCCTCGGCGCGCCGGTCGGCGCATCGCTCGCCGGTTCACGCGCCTTCATCGACGAGGTCTGGCGCTGGAAGCAGCGG
CTCGGCGGTTCGATGCGGCAGGCCGGAATCATCGCCGCCGCCTGCCACTACGCGCTCGATCATCACGTCGAACGCCTGGC
GGAGGATCACGCCAACGCCCGCGCGCTGGCCGCAGGGCTGGCGCAGATCGCCGGCGTCGATGTGCAGCAGCCGCACACCA
ATCTGGTGTTCTTCCGGCCCGACGGCGCGGGCGTTGCCGGCGACGCGATGGTCGCCGAGCTGCGGCAGCGCGGCGTGCTG
CTGGCGATGATGGACGGCCGGATCCGCGCCTGCACCCATCTCGACGTCAGCGCCGCGATGATCGACGAGACGCTGGCGCT
GGTGCGCGAGATCGTGCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGGCGCGCTGCGTAGCGTCCGGGGCGCCAAAACTCGATCTCGTCTGCGAGATCACGGCCATTCCTCGCTCCGCCGCC
GCCGTGCTAATCTGCGCGCC

Downstream 100 bases:

>100_bases
ACGAAAAACGCGCGCCGCTCGGAGAGCAGCGCGCGTCGATGACTTCGTTTTCACACGCGCCGCCGAGAGCGGCGTGTGAG
CACCGTCACAGGCAGAGGCG

Product: aromatic amino acid beta-eliminating lyase/threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAYTPAPPDPNAPPVRINLLSDTQTRPTPAMREAMARAAVGDEQTGDDPTVNALNERVAALLGKEAAVFLPSGTMCNVTA
TLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLDAFRAALHPRSRYEPPQTMVSVEQTANIGGGTI
WPQATLDAIAAAGKEAGLATHMDGARLMNAAVATSIAAHEMAAGWDSAWIDFSKGLGAPVGASLAGSRAFIDEVWRWKQR
LGGSMRQAGIIAAACHYALDHHVERLAEDHANARALAAGLAQIAGVDVQQPHTNLVFFRPDGAGVAGDAMVAELRQRGVL
LAMMDGRIRACTHLDVSAAMIDETLALVREIVRKA

Sequences:

>Translated_355_residues
MAYTPAPPDPNAPPVRINLLSDTQTRPTPAMREAMARAAVGDEQTGDDPTVNALNERVAALLGKEAAVFLPSGTMCNVTA
TLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLDAFRAALHPRSRYEPPQTMVSVEQTANIGGGTI
WPQATLDAIAAAGKEAGLATHMDGARLMNAAVATSIAAHEMAAGWDSAWIDFSKGLGAPVGASLAGSRAFIDEVWRWKQR
LGGSMRQAGIIAAACHYALDHHVERLAEDHANARALAAGLAQIAGVDVQQPHTNLVFFRPDGAGVAGDAMVAELRQRGVL
LAMMDGRIRACTHLDVSAAMIDETLALVREIVRKA
>Mature_354_residues
AYTPAPPDPNAPPVRINLLSDTQTRPTPAMREAMARAAVGDEQTGDDPTVNALNERVAALLGKEAAVFLPSGTMCNVTAT
LATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDGDDGQFSLDAFRAALHPRSRYEPPQTMVSVEQTANIGGGTIW
PQATLDAIAAAGKEAGLATHMDGARLMNAAVATSIAAHEMAAGWDSAWIDFSKGLGAPVGASLAGSRAFIDEVWRWKQRL
GGSMRQAGIIAAACHYALDHHVERLAEDHANARALAAGLAQIAGVDVQQPHTNLVFFRPDGAGVAGDAMVAELRQRGVLL
AMMDGRIRACTHLDVSAAMIDETLALVREIVRKA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=333, Percent_Identity=42.9429429429429, Blast_Score=221, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI193206524, Length=362, Percent_Identity=35.9116022099448, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193206522, Length=362, Percent_Identity=35.9116022099448, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193206520, Length=362, Percent_Identity=35.9116022099448, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320789, Length=326, Percent_Identity=30.0613496932515, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355439, Length=294, Percent_Identity=41.8367346938776, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 37267; Mature: 37135

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTPAPPDPNAPPVRINLLSDTQTRPTPAMREAMARAAVGDEQTGDDPTVNALNERVAA
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LLGKEAAVFLPSGTMCNVTATLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDG
HHCCCCEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
DDGQFSLDAFRAALHPRSRYEPPQTMVSVEQTANIGGGTIWPQATLDAIAAAGKEAGLAT
CCCCCHHHHHHHHHCCCCCCCCHHHHEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEE
HMDGARLMNAAVATSIAAHEMAAGWDSAWIDFSKGLGAPVGASLAGSRAFIDEVWRWKQR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHH
LGGSMRQAGIIAAACHYALDHHVERLAEDHANARALAAGLAQIAGVDVQQPHTNLVFFRP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DGAGVAGDAMVAELRQRGVLLAMMDGRIRACTHLDVSAAMIDETLALVREIVRKA
CCCCCCHHHHHHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYTPAPPDPNAPPVRINLLSDTQTRPTPAMREAMARAAVGDEQTGDDPTVNALNERVAA
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LLGKEAAVFLPSGTMCNVTATLATCRPGDEIIAHRTAHILSREGGAHAALGGFQITGLDG
HHCCCCEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
DDGQFSLDAFRAALHPRSRYEPPQTMVSVEQTANIGGGTIWPQATLDAIAAAGKEAGLAT
CCCCCHHHHHHHHHCCCCCCCCHHHHEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEE
HMDGARLMNAAVATSIAAHEMAAGWDSAWIDFSKGLGAPVGASLAGSRAFIDEVWRWKQR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHH
LGGSMRQAGIIAAACHYALDHHVERLAEDHANARALAAGLAQIAGVDVQQPHTNLVFFRP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DGAGVAGDAMVAELRQRGVLLAMMDGRIRACTHLDVSAAMIDETLALVREIVRKA
CCCCCCHHHHHHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]