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The map label for this gene is paaI [H]

Identifier: 86750745

GI number: 86750745

Start: 4171980

End: 4172453

Strand: Reverse

Name: paaI [H]

Synonym: RPB_3635

Alternate gene names: 86750745

Gene position: 4172453-4171980 (Counterclockwise)

Preceding gene: 86750747

Following gene: 86750744

Centisome position: 78.26

GC content: 66.88

Gene sequence:

>474_bases
GTGACCGTGGCGCGCTCCCCCGCTGCCGCGGAGTTGTCGCCCGACGAATTGGCGCGCGCCTGCGCCGACGCGATGTGGGC
CAACGATCATGCCAGCAAGGCGCTCGGCATGGAGATCGTCGAGATCGGCCCCGGCTTCGCGACGCTGGCGATGACGATCA
CCACCGAGATGGTGAACGGCCACGGCATCGCCCATGGCGGCTTCATCTTTACGCTGGCCGACTCCGCCTTCGCCTATGCC
TGCAACTCGCGCAACGAGAACACCGTGGCGGCGCAGGGCCAGATCAGTTTCATCCGCCCGAGCAAGCTCGGCGCGAAGCT
GGTGGCAAGCGCGCGCGAAGTCACCCGCGCCGGACGATCCGGCATCTTCGATGTCCGCGTTTCCTCGGCCGGAATCGTGA
TCGCCGAATTCCGTGGCCATTCGCGGTCGATCGGCGGAACGCTGGTGCCGCTGCAACAACCCGATCAACGCTGA

Upstream 100 bases:

>100_bases
GTGGTGGGTGAAATTGGGAGCGAAACGTGAATGACGACATGATCGCCGACCGGAAGCGGCGACGCATCGCGATGCGTGCC
TGCCGCGCTCGGACCATGCG

Downstream 100 bases:

>100_bases
GAGAGGATAGAGCGATGGCCGCGAGCATCCATCAATTGCGGGCACCTTCCGGCTACAGCGCCGAGCTCGACGAAGCCGAG
CGCGCCTCCCGCGACGCGAT

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTVARSPAAAELSPDELARACADAMWANDHASKALGMEIVEIGPGFATLAMTITTEMVNGHGIAHGGFIFTLADSAFAYA
CNSRNENTVAAQGQISFIRPSKLGAKLVASAREVTRAGRSGIFDVRVSSAGIVIAEFRGHSRSIGGTLVPLQQPDQR

Sequences:

>Translated_157_residues
MTVARSPAAAELSPDELARACADAMWANDHASKALGMEIVEIGPGFATLAMTITTEMVNGHGIAHGGFIFTLADSAFAYA
CNSRNENTVAAQGQISFIRPSKLGAKLVASAREVTRAGRSGIFDVRVSSAGIVIAEFRGHSRSIGGTLVPLQQPDQR
>Mature_156_residues
TVARSPAAAELSPDELARACADAMWANDHASKALGMEIVEIGPGFATLAMTITTEMVNGHGIAHGGFIFTLADSAFAYAC
NSRNENTVAAQGQISFIRPSKLGAKLVASAREVTRAGRSGIFDVRVSSAGIVIAEFRGHSRSIGGTLVPLQQPDQR

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=128, Percent_Identity=44.53125, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16429; Mature: 16298

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARSPAAAELSPDELARACADAMWANDHASKALGMEIVEIGPGFATLAMTITTEMVNG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEECCCHHHEEEEEEEEHHCC
HGIAHGGFIFTLADSAFAYACNSRNENTVAAQGQISFIRPSKLGAKLVASAREVTRAGRS
CCEECCCEEEEEECCCEEEEECCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHCCCC
GIFDVRVSSAGIVIAEFRGHSRSIGGTLVPLQQPDQR
CEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TVARSPAAAELSPDELARACADAMWANDHASKALGMEIVEIGPGFATLAMTITTEMVNG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEECCCHHHEEEEEEEEHHCC
HGIAHGGFIFTLADSAFAYACNSRNENTVAAQGQISFIRPSKLGAKLVASAREVTRAGRS
CCEECCCEEEEEECCCEEEEECCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHCCCC
GIFDVRVSSAGIVIAEFRGHSRSIGGTLVPLQQPDQR
CEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]