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The map label for this gene is gatA [H]

Identifier: 86750696

GI number: 86750696

Start: 4110253

End: 4111602

Strand: Reverse

Name: gatA [H]

Synonym: RPB_3586

Alternate gene names: 86750696

Gene position: 4111602-4110253 (Counterclockwise)

Preceding gene: 86750700

Following gene: 86750695

Centisome position: 77.12

GC content: 70.15

Gene sequence:

>1350_bases
ATGACGAAATTGCCCACGCTCGCAGTCCTCGCCGCCGATCTCGACGCCGGCCGCACCACCGCGCGCAAACTGGTCGACGA
TTGCCTGGCGCGGATCGACGACGCGGCGGGCGAGGGCGCGCGCGCCTTCATTCATGTCGATCGCGATGCGGCGCGCGAAG
CCGCCGAGGCGATGGACAAGCTGCGCGCGATCAAGGCGGCGCCGTCGCCCTATGCCGGCATTCCGGTTTCGATCAAGGAC
CTGTTCGATATCAGGGGCCAGGTCACCCGCGCCGGCTCGCGCGCGCTCGACGACAATCCGCCGGCGGAGGACGACGCCCC
CGCGGTGGCGCGGCTGCGGCGCGCCGGCTTCGTGGTGATCGGCCGCACCAACATGACCGAGTTCGCCTATTCCGGCATCG
GCATCAATCCGCATTACGGCACGCCGAAATCGGCCTATGGCCGCGACGTCGGCTACGTGCCGGGCGGCTCGTCCTCGGGC
GCGGCGGTGTCGGTCGCCGACGGCATGGCGCATGCCGGGCTCGGCACCGACACCGGCGGCTCGTGCCGGATTCCGGCGGC
GTTCAACGGCATCGTCGGCTACAAGCCGTCGCAGCAACGCGTGCCGCGCGACGGCGCGGTGCCGCTGTCGTTCTCGCTGG
ATTCGATCGGGCCGCTGGCGCGCAGCGTCGCCTGCTGCGCGGTGCTCGACGCCGTGCTCGCCGACGAAGAGATCGTGCCG
CTGGCGCCGCGGCCACTGAAGGGGCTGCGGCTGGCGGTGCCGACCACGGTGGCGCTCGACGAACTCGATGAAGCGGTGAG
ACTCGCGTTCGAGCGCGCGCTGAAGGCGCTGGCCGGCCACGGCGCGATCATCGAGAAGATCGAGGTGCCGGAATTCAACG
ACGTGGCACCCCTCAGCGCCAAAGGCGGCCTCACCGCGGCGGAGAGCTACGCCTGGCATCGCTATCTGATCGTGGCGCAA
GGCGACGTCTACGACCCGCGGGTGCGCGAGCGCATCCTGCGCGGCGAGAGCCAGAGCGCCGCGGACTATGTCGACACCAT
CACGGCGCGCAAATCGCTGATCGCGCGCGCCACCACGCGGCTCGCGCCCTACGATGCAGTGGTGATGCCGACCACGGCCA
ACACGCCGCCGGCCATCGCCGACCTCGCCGACGACAAGGCGTTCACCAAGGCCAATCTGCTGGCGTTGCGCAATTGCACG
CTCATCAACATGATCGACGGCTGCGCGATCTCGCTGCCGTGCCATCAGGACGGCGAAGCGCCGGTCGGGCTGATGGTGGC
CGGCGTCAACGGCGCCGATCGCGACGTTTTCGAAGTGGCGGCGGGCGTGGAGGCGGTGCTTCGTGGTTGA

Upstream 100 bases:

>100_bases
GGACTCCGCGCTACAGGAGACGCCAAGCAACCCTCAGTTCGGCGAAGCCGTGCGCGCCCAGACCGGCGTCGCCGGTCGAC
CTTCCAGCGGAATAGACGCC

Downstream 100 bases:

>100_bases
TCTGTCGTTCATCCTCGACGCGCAGGGCGCGCGCACGCCGCTGACGCTGCCGATCTCGCAGGCCGTGATCGCCGGCTGGA
CCGGGCGCGATCCGGTGGCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTKLPTLAVLAADLDAGRTTARKLVDDCLARIDDAAGEGARAFIHVDRDAAREAAEAMDKLRAIKAAPSPYAGIPVSIKD
LFDIRGQVTRAGSRALDDNPPAEDDAPAVARLRRAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAYGRDVGYVPGGSSSG
AAVSVADGMAHAGLGTDTGGSCRIPAAFNGIVGYKPSQQRVPRDGAVPLSFSLDSIGPLARSVACCAVLDAVLADEEIVP
LAPRPLKGLRLAVPTTVALDELDEAVRLAFERALKALAGHGAIIEKIEVPEFNDVAPLSAKGGLTAAESYAWHRYLIVAQ
GDVYDPRVRERILRGESQSAADYVDTITARKSLIARATTRLAPYDAVVMPTTANTPPAIADLADDKAFTKANLLALRNCT
LINMIDGCAISLPCHQDGEAPVGLMVAGVNGADRDVFEVAAGVEAVLRG

Sequences:

>Translated_449_residues
MTKLPTLAVLAADLDAGRTTARKLVDDCLARIDDAAGEGARAFIHVDRDAAREAAEAMDKLRAIKAAPSPYAGIPVSIKD
LFDIRGQVTRAGSRALDDNPPAEDDAPAVARLRRAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAYGRDVGYVPGGSSSG
AAVSVADGMAHAGLGTDTGGSCRIPAAFNGIVGYKPSQQRVPRDGAVPLSFSLDSIGPLARSVACCAVLDAVLADEEIVP
LAPRPLKGLRLAVPTTVALDELDEAVRLAFERALKALAGHGAIIEKIEVPEFNDVAPLSAKGGLTAAESYAWHRYLIVAQ
GDVYDPRVRERILRGESQSAADYVDTITARKSLIARATTRLAPYDAVVMPTTANTPPAIADLADDKAFTKANLLALRNCT
LINMIDGCAISLPCHQDGEAPVGLMVAGVNGADRDVFEVAAGVEAVLRG
>Mature_448_residues
TKLPTLAVLAADLDAGRTTARKLVDDCLARIDDAAGEGARAFIHVDRDAAREAAEAMDKLRAIKAAPSPYAGIPVSIKDL
FDIRGQVTRAGSRALDDNPPAEDDAPAVARLRRAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAYGRDVGYVPGGSSSGA
AVSVADGMAHAGLGTDTGGSCRIPAAFNGIVGYKPSQQRVPRDGAVPLSFSLDSIGPLARSVACCAVLDAVLADEEIVPL
APRPLKGLRLAVPTTVALDELDEAVRLAFERALKALAGHGAIIEKIEVPEFNDVAPLSAKGGLTAAESYAWHRYLIVAQG
DVYDPRVRERILRGESQSAADYVDTITARKSLIARATTRLAPYDAVVMPTTANTPPAIADLADDKAFTKANLLALRNCTL
INMIDGCAISLPCHQDGEAPVGLMVAGVNGADRDVFEVAAGVEAVLRG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=198, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=24.8015873015873, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI166795287, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=28.3185840707965, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=179, Percent_Identity=32.9608938547486, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=34.5744680851064, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=164, Percent_Identity=34.7560975609756, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=161, Percent_Identity=36.6459627329193, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=161, Percent_Identity=36.6459627329193, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538254, Length=216, Percent_Identity=28.7037037037037, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=384, Percent_Identity=27.6041666666667, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=25.7575757575758, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=26.3265306122449, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=187, Percent_Identity=32.620320855615, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648435, Length=188, Percent_Identity=32.4468085106383, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648437, Length=188, Percent_Identity=32.4468085106383, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648441, Length=188, Percent_Identity=32.4468085106383, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648439, Length=188, Percent_Identity=32.4468085106383, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652985, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922090, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652981, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652983, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=23.8834951456311, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644968, Length=488, Percent_Identity=22.3360655737705, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46928; Mature: 46797

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLPTLAVLAADLDAGRTTARKLVDDCLARIDDAAGEGARAFIHVDRDAAREAAEAMDK
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
LRAIKAAPSPYAGIPVSIKDLFDIRGQVTRAGSRALDDNPPAEDDAPAVARLRRAGFVVI
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEEE
GRTNMTEFAYSGIGINPHYGTPKSAYGRDVGYVPGGSSSGAAVSVADGMAHAGLGTDTGG
ECCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCC
SCRIPAAFNGIVGYKPSQQRVPRDGAVPLSFSLDSIGPLARSVACCAVLDAVLADEEIVP
CEECCHHHCCCCCCCCCHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAPRPLKGLRLAVPTTVALDELDEAVRLAFERALKALAGHGAIIEKIEVPEFNDVAPLSA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
KGGLTAAESYAWHRYLIVAQGDVYDPRVRERILRGESQSAADYVDTITARKSLIARATTR
CCCCCHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAPYDAVVMPTTANTPPAIADLADDKAFTKANLLALRNCTLINMIDGCAISLPCHQDGEA
CCCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCCCC
PVGLMVAGVNGADRDVFEVAAGVEAVLRG
CEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKLPTLAVLAADLDAGRTTARKLVDDCLARIDDAAGEGARAFIHVDRDAAREAAEAMDK
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
LRAIKAAPSPYAGIPVSIKDLFDIRGQVTRAGSRALDDNPPAEDDAPAVARLRRAGFVVI
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEEE
GRTNMTEFAYSGIGINPHYGTPKSAYGRDVGYVPGGSSSGAAVSVADGMAHAGLGTDTGG
ECCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCC
SCRIPAAFNGIVGYKPSQQRVPRDGAVPLSFSLDSIGPLARSVACCAVLDAVLADEEIVP
CEECCHHHCCCCCCCCCHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAPRPLKGLRLAVPTTVALDELDEAVRLAFERALKALAGHGAIIEKIEVPEFNDVAPLSA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
KGGLTAAESYAWHRYLIVAQGDVYDPRVRERILRGESQSAADYVDTITARKSLIARATTR
CCCCCHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAPYDAVVMPTTANTPPAIADLADDKAFTKANLLALRNCTLINMIDGCAISLPCHQDGEA
CCCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCCCC
PVGLMVAGVNGADRDVFEVAAGVEAVLRG
CEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA