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The map label for this gene is vsr [H]

Identifier: 86750434

GI number: 86750434

Start: 3802018

End: 3802440

Strand: Reverse

Name: vsr [H]

Synonym: RPB_3323

Alternate gene names: 86750434

Gene position: 3802440-3802018 (Counterclockwise)

Preceding gene: 86750435

Following gene: 86750432

Centisome position: 71.32

GC content: 56.74

Gene sequence:

>423_bases
ATGGGAATTAGTCGCAGCGAGAACATGCGGCGCATCCGCAGCAAAGATACCGCACCGGAGATGGTCGTGCGCCGTTTAGT
GCATGGCATGGGATACCGCTACCGTCTGCATCGCAAGGACATTCCGGGCAAACCCGATCTCGCTCTTCTCAGCCGCAAAA
AAGCAATCCTCGTTCATGGTTGTTTCTGGCATCAGCACCCGGGATGCCGGGAGGGGCGGCCGCCCAAATCTAACCGCGAG
TATTGGCTTCCAAAGCTAAAACGCAACCAAGATCGCGACAGGGCTGCGCTTGTGAACCTTGCCGATTTGGGCTGGCGGGT
GCTTGTGGTTTGGGAATGCGAAACGAAAGATGCCGTTGTCTTGCGAGAGAGGCTGGAGACGTTTTTGGAGCAGCCAGAAC
GTGTCCATCCATTCGCTGGCTAG

Upstream 100 bases:

>100_bases
GAAGCTTCGTCCGTCGAGAGAGTCCTGCAGCACATTGAGGAGGGGCGTTTGATCAGCCTGCAGAAGTGGCTCGATAAAAT
CGCAGGATAGCAGCTAGTAC

Downstream 100 bases:

>100_bases
GAATGACTTCTGGGGCTTTATCGCCTTGTGGCAGCGGCGTGCCGACTTTGTCTTATTGGCATCCGTCAACCTCAATCCAA
TCCGGCCAAGGCTGTCATCA

Product: DNA mismatch endonuclease vsr

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MGISRSENMRRIRSKDTAPEMVVRRLVHGMGYRYRLHRKDIPGKPDLALLSRKKAILVHGCFWHQHPGCREGRPPKSNRE
YWLPKLKRNQDRDRAALVNLADLGWRVLVVWECETKDAVVLRERLETFLEQPERVHPFAG

Sequences:

>Translated_140_residues
MGISRSENMRRIRSKDTAPEMVVRRLVHGMGYRYRLHRKDIPGKPDLALLSRKKAILVHGCFWHQHPGCREGRPPKSNRE
YWLPKLKRNQDRDRAALVNLADLGWRVLVVWECETKDAVVLRERLETFLEQPERVHPFAG
>Mature_139_residues
GISRSENMRRIRSKDTAPEMVVRRLVHGMGYRYRLHRKDIPGKPDLALLSRKKAILVHGCFWHQHPGCREGRPPKSNREY
WLPKLKRNQDRDRAALVNLADLGWRVLVVWECETKDAVVLRERLETFLEQPERVHPFAG

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=131, Percent_Identity=40.4580152671756, Blast_Score=105, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16501; Mature: 16370

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISRSENMRRIRSKDTAPEMVVRRLVHGMGYRYRLHRKDIPGKPDLALLSRKKAILVHG
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCEEEEEE
CFWHQHPGCREGRPPKSNREYWLPKLKRNQDRDRAALVNLADLGWRVLVVWECETKDAVV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHH
LRERLETFLEQPERVHPFAG
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GISRSENMRRIRSKDTAPEMVVRRLVHGMGYRYRLHRKDIPGKPDLALLSRKKAILVHG
CCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCEEEEEE
CFWHQHPGCREGRPPKSNREYWLPKLKRNQDRDRAALVNLADLGWRVLVVWECETKDAVV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHH
LRERLETFLEQPERVHPFAG
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]