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The map label for this gene is yfcG [H]

Identifier: 86750320

GI number: 86750320

Start: 3670594

End: 3671313

Strand: Reverse

Name: yfcG [H]

Synonym: RPB_3209

Alternate gene names: 86750320

Gene position: 3671313-3670594 (Counterclockwise)

Preceding gene: 86750323

Following gene: 86750319

Centisome position: 68.86

GC content: 65.69

Gene sequence:

>720_bases
ATGATCGAGCTGTTTTACGCGCCGACGCCGAACGGCTGGAAGATCTCGATCATGCTGGAAGAATGCGGCCTGCCGTACAA
AGTGGTGCCGATGCAGCTCGGCCGCGGCGATCAGTTGAAGCCGGAGTTTCTCGCGCTCAGCCCGAACGGCCGGATGCCGG
CGATCGTCGATCACGCGCCCGCCGACGGCGCCGGGCCGCTGAGCATGTTCGAGAGCGGCGCGATCCTGATCTATCTGGCG
GAGAAGACCGGACGCTTCATGCCCGCCGAGGCACGGCGCCGCTTCGACGTGATGCAATGGGTGATGTGGCAGATGGCCGG
CTTGGGGCCGATGCTCGGCCAGAACGGCCATTTCCTGCTCTATGCGCCGGAGAAAATTCCCTACGCGATCGAGCGCTACA
GCCGCGAAGCGAAGCGACTGTACGGCGTGCTCGACGCGCAGCTCGAAGGCCGCGATCACATCGCCGGCGACTATTCGATC
GCCGACATCGCCTGCTTTCCGTGGATCATGACTCACAAGGCGCAGGGCCTGACGCTCGACGACTATCCGAACGTGAAGCG
CTGGTACGCGGCGCTCCGCGCGCGGCCGAAGCTGCAGGCTGGCCTCGCGCTCGGCAAGGAGGACAGGAAGCCGATGGACG
AACAGGCGCGCAAGCTGCTGTTCGGCGTCGACAAGCCGGCGCATGCCGATGCGGCTGTGCAGGGCGCGCAGCAGCAGTGA

Upstream 100 bases:

>100_bases
CCATGTTCGCTGCGTGGCGGATAGGGGGCCGAGACCGAGGTGACAAGGCCGGCGCGGCCGGGCTAGGATGCGGAAAAATC
AACCCGTGAAGGGAGCCCGC

Downstream 100 bases:

>100_bases
TGGCGAGTCGTGGTGAGCACTGGATACGGACGACGTTGATGCACGCGCGATCAACAACCACACCCGTCATCGCCCGGCTC
GACCGGGCGACCCAGTATCC

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIELFYAPTPNGWKISIMLEECGLPYKVVPMQLGRGDQLKPEFLALSPNGRMPAIVDHAPADGAGPLSMFESGAILIYLA
EKTGRFMPAEARRRFDVMQWVMWQMAGLGPMLGQNGHFLLYAPEKIPYAIERYSREAKRLYGVLDAQLEGRDHIAGDYSI
ADIACFPWIMTHKAQGLTLDDYPNVKRWYAALRARPKLQAGLALGKEDRKPMDEQARKLLFGVDKPAHADAAVQGAQQQ

Sequences:

>Translated_239_residues
MIELFYAPTPNGWKISIMLEECGLPYKVVPMQLGRGDQLKPEFLALSPNGRMPAIVDHAPADGAGPLSMFESGAILIYLA
EKTGRFMPAEARRRFDVMQWVMWQMAGLGPMLGQNGHFLLYAPEKIPYAIERYSREAKRLYGVLDAQLEGRDHIAGDYSI
ADIACFPWIMTHKAQGLTLDDYPNVKRWYAALRARPKLQAGLALGKEDRKPMDEQARKLLFGVDKPAHADAAVQGAQQQ
>Mature_239_residues
MIELFYAPTPNGWKISIMLEECGLPYKVVPMQLGRGDQLKPEFLALSPNGRMPAIVDHAPADGAGPLSMFESGAILIYLA
EKTGRFMPAEARRRFDVMQWVMWQMAGLGPMLGQNGHFLLYAPEKIPYAIERYSREAKRLYGVLDAQLEGRDHIAGDYSI
ADIACFPWIMTHKAQGLTLDDYPNVKRWYAALRARPKLQAGLALGKEDRKPMDEQARKLLFGVDKPAHADAAVQGAQQQ

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=197, Percent_Identity=56.8527918781726, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082195, Length=249, Percent_Identity=37.7510040160643, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI87081789, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=30.0429184549356, Blast_Score=115, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571670, Length=176, Percent_Identity=27.8409090909091, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45549270, Length=194, Percent_Identity=29.3814432989691, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864592, Length=193, Percent_Identity=30.5699481865285, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17864594, Length=198, Percent_Identity=29.7979797979798, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17933730, Length=206, Percent_Identity=28.1553398058252, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI85725204, Length=194, Percent_Identity=28.3505154639175, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737923, Length=194, Percent_Identity=28.3505154639175, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646249, Length=186, Percent_Identity=29.5698924731183, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26730; Mature: 26730

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELFYAPTPNGWKISIMLEECGLPYKVVPMQLGRGDQLKPEFLALSPNGRMPAIVDHAP
CEEEEECCCCCCEEEEEEEHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
ADGAGPLSMFESGAILIYLAEKTGRFMPAEARRRFDVMQWVMWQMAGLGPMLGQNGHFLL
CCCCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YAPEKIPYAIERYSREAKRLYGVLDAQLEGRDHIAGDYSIADIACFPWIMTHKAQGLTLD
ECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DYPNVKRWYAALRARPKLQAGLALGKEDRKPMDEQARKLLFGVDKPAHADAAVQGAQQQ
CCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MIELFYAPTPNGWKISIMLEECGLPYKVVPMQLGRGDQLKPEFLALSPNGRMPAIVDHAP
CEEEEECCCCCCEEEEEEEHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
ADGAGPLSMFESGAILIYLAEKTGRFMPAEARRRFDVMQWVMWQMAGLGPMLGQNGHFLL
CCCCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YAPEKIPYAIERYSREAKRLYGVLDAQLEGRDHIAGDYSIADIACFPWIMTHKAQGLTLD
ECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DYPNVKRWYAALRARPKLQAGLALGKEDRKPMDEQARKLLFGVDKPAHADAAVQGAQQQ
CCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]