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The map label for this gene is hipO [H]

Identifier: 86750200

GI number: 86750200

Start: 3517479

End: 3518648

Strand: Reverse

Name: hipO [H]

Synonym: RPB_3086

Alternate gene names: 86750200

Gene position: 3518648-3517479 (Counterclockwise)

Preceding gene: 86750201

Following gene: 86750199

Centisome position: 66.0

GC content: 64.27

Gene sequence:

>1170_bases
ATGCCGATTGAGAACTGGACCGCGCGCTACCTGGACGAACTCAAGGAGTTTCGTCACGATCTGCACCGCAACCCCGAGTT
GCTGTACGACGTTCATCGCACCGCCGAGCGGGTCGCGGCGCGGTTGCGCGAAGCCGGCGTCGACGAGGTGCACGAAGGCA
TCGGCGGAACGGGCGTCGTCGGAATCATTTACGGCCAATCGCGTAGTTCCGGCCGGATGATCGGGCTGCGCGCCGATATG
GATGCTTTGCCCATTCTCGAGGCAACTGGCGCGGAATGGGCCTCGCAAGTCCCCGGCAAGATGCACGCATGCGGCCACGA
CGGCCACACCACCATGCTGTTGGGTGCGGCGCTTGGCCTCGTCGAGAGCCGGGCCTTCGACGGCGGCGTCGCGCTGATCT
TCCAGCCCGCCGAAGAAGGCGGTGCGGGCGCCAAGGCCATGCTCGATGATGGCCTGCTGCAGCGCTTTCCGATCCAGGAA
TTCTACGGGATGCACAACCGCCCGGGACTTCCCTTGGGGACGTTTGCGACGGGCCCAGGGCCGCAAATGGGTTCGGTCGA
CGAGATCATCATCTCGATCGAAGGTCGCGGCGGCCACGCCGCTCAGCCGCATGCCACGGTCGATCCGGTGGTCGTTGCCG
CGGCGTTGATTCAGGCGACTCAGGCGATCGTCTCGCGCAATCTCGATCCACTGCAATCCGCGGTTATTTCGATCACACAG
ATGACCGCCGGTGACGCGTTCAACGTCATTCCTCAAACGGTGACATTGCGCGGCACGGTTCGCACGCTGGACGAGCCGAC
CCGCGACATGGTTGAAAAGCGGCTGCGCGAACTCACCGAGAGTATTTCGGCGGGGTTCAGCGCTGTCGGTACGCTGTCTT
ATCTGCGGCACTACCCTGTGATGAGAAATTCCGAAGTCGGCGTCGACCGCGCGGTCGCGGCGGCCGGAGAAGTGGCCGGC
GTCGCGCACGTCGACGCCACAATGGCTCCGACGCTGGGCGGCGAGGACTTCGCGTTCATGCTGAACGAGCGGCCTGGCGC
GATGATCATGATCGGTAACGGCGATAGCGCCCCGCTGCATCATCCGCGCTTCGATTTCAACGACGACGTCATCCCGTGGG
GCTGTTCGTATTGGACCGCCTTGGTCCGCCAGCGCATGCCGTTGGTCTGA

Upstream 100 bases:

>100_bases
TTTGAATGTGCGAGCCGGCCGACCCCACGCGCGCGCAATGCGAGCGAAGACGGCGTGTGAATTTGACCTTGCTGTGATTG
GCGAACCTCAGGAACCTCAA

Downstream 100 bases:

>100_bases
ACGCGGTGCATCGAAGAACGGACATTCTGAAAAATGCAGCGCGCAGAGATCGTCTATCTCGGAACGCCGACAAATTTGGA
GAACAGCGTCCTCGGATTTT

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPIENWTARYLDELKEFRHDLHRNPELLYDVHRTAERVAARLREAGVDEVHEGIGGTGVVGIIYGQSRSSGRMIGLRADM
DALPILEATGAEWASQVPGKMHACGHDGHTTMLLGAALGLVESRAFDGGVALIFQPAEEGGAGAKAMLDDGLLQRFPIQE
FYGMHNRPGLPLGTFATGPGPQMGSVDEIIISIEGRGGHAAQPHATVDPVVVAAALIQATQAIVSRNLDPLQSAVISITQ
MTAGDAFNVIPQTVTLRGTVRTLDEPTRDMVEKRLRELTESISAGFSAVGTLSYLRHYPVMRNSEVGVDRAVAAAGEVAG
VAHVDATMAPTLGGEDFAFMLNERPGAMIMIGNGDSAPLHHPRFDFNDDVIPWGCSYWTALVRQRMPLV

Sequences:

>Translated_389_residues
MPIENWTARYLDELKEFRHDLHRNPELLYDVHRTAERVAARLREAGVDEVHEGIGGTGVVGIIYGQSRSSGRMIGLRADM
DALPILEATGAEWASQVPGKMHACGHDGHTTMLLGAALGLVESRAFDGGVALIFQPAEEGGAGAKAMLDDGLLQRFPIQE
FYGMHNRPGLPLGTFATGPGPQMGSVDEIIISIEGRGGHAAQPHATVDPVVVAAALIQATQAIVSRNLDPLQSAVISITQ
MTAGDAFNVIPQTVTLRGTVRTLDEPTRDMVEKRLRELTESISAGFSAVGTLSYLRHYPVMRNSEVGVDRAVAAAGEVAG
VAHVDATMAPTLGGEDFAFMLNERPGAMIMIGNGDSAPLHHPRFDFNDDVIPWGCSYWTALVRQRMPLV
>Mature_388_residues
PIENWTARYLDELKEFRHDLHRNPELLYDVHRTAERVAARLREAGVDEVHEGIGGTGVVGIIYGQSRSSGRMIGLRADMD
ALPILEATGAEWASQVPGKMHACGHDGHTTMLLGAALGLVESRAFDGGVALIFQPAEEGGAGAKAMLDDGLLQRFPIQEF
YGMHNRPGLPLGTFATGPGPQMGSVDEIIISIEGRGGHAAQPHATVDPVVVAAALIQATQAIVSRNLDPLQSAVISITQM
TAGDAFNVIPQTVTLRGTVRTLDEPTRDMVEKRLRELTESISAGFSAVGTLSYLRHYPVMRNSEVGVDRAVAAAGEVAGV
AHVDATMAPTLGGEDFAFMLNERPGAMIMIGNGDSAPLHHPRFDFNDDVIPWGCSYWTALVRQRMPLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=386, Percent_Identity=27.720207253886, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41742; Mature: 41611

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIENWTARYLDELKEFRHDLHRNPELLYDVHRTAERVAARLREAGVDEVHEGIGGTGVV
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GIIYGQSRSSGRMIGLRADMDALPILEATGAEWASQVPGKMHACGHDGHTTMLLGAALGL
EEEEECCCCCCCEEEEECCCCCCCCHHHCCCHHHHHCCCCEECCCCCCCHHHHHHHHHHH
VESRAFDGGVALIFQPAEEGGAGAKAMLDDGLLQRFPIQEFYGMHNRPGLPLGTFATGPG
HHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCC
PQMGSVDEIIISIEGRGGHAAQPHATVDPVVVAAALIQATQAIVSRNLDPLQSAVISITQ
CCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MTAGDAFNVIPQTVTLRGTVRTLDEPTRDMVEKRLRELTESISAGFSAVGTLSYLRHYPV
HCCCCCHHCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRNSEVGVDRAVAAAGEVAGVAHVDATMAPTLGGEDFAFMLNERPGAMIMIGNGDSAPLH
CCCCCCCHHHHHHHHHHHCCCEECCCHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCC
HPRFDFNDDVIPWGCSYWTALVRQRMPLV
CCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIENWTARYLDELKEFRHDLHRNPELLYDVHRTAERVAARLREAGVDEVHEGIGGTGVV
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GIIYGQSRSSGRMIGLRADMDALPILEATGAEWASQVPGKMHACGHDGHTTMLLGAALGL
EEEEECCCCCCCEEEEECCCCCCCCHHHCCCHHHHHCCCCEECCCCCCCHHHHHHHHHHH
VESRAFDGGVALIFQPAEEGGAGAKAMLDDGLLQRFPIQEFYGMHNRPGLPLGTFATGPG
HHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCC
PQMGSVDEIIISIEGRGGHAAQPHATVDPVVVAAALIQATQAIVSRNLDPLQSAVISITQ
CCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MTAGDAFNVIPQTVTLRGTVRTLDEPTRDMVEKRLRELTESISAGFSAVGTLSYLRHYPV
HCCCCCHHCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRNSEVGVDRAVAAAGEVAGVAHVDATMAPTLGGEDFAFMLNERPGAMIMIGNGDSAPLH
CCCCCCCHHHHHHHHHHHCCCEECCCHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCC
HPRFDFNDDVIPWGCSYWTALVRQRMPLV
CCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]