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The map label for this gene is lcfB [H]

Identifier: 86750191

GI number: 86750191

Start: 3507825

End: 3509363

Strand: Reverse

Name: lcfB [H]

Synonym: RPB_3077

Alternate gene names: 86750191

Gene position: 3509363-3507825 (Counterclockwise)

Preceding gene: 86750192

Following gene: 86750190

Centisome position: 65.82

GC content: 64.78

Gene sequence:

>1539_bases
ATGAGCTTCCGATGGAAGAACCTCGGAGATTTGGTGGATCGCTCGCGCGGTCTCGATCGTCCCGCGATCATCGATCTGCG
GTCCCCGGAGCCGAGGGTCTGGACCCATAGCGAGATCGATAGGCTCGGAAATGGCGTGGCGTCTTATTTGACCGGGCTTG
GCTTGGCGCGTGGAACGACGGTCGCCATTCTCTCGCTCAACCGTGCGGAGTACATTGCGGCGTATTTCGGCATCATGCGC
GCCGGCTTCGTCGCGCTGCCGGTCAACACCAAGCAGCCGGCCGAAACGATCGATTTTGTGCTGCGCGACTCCGACACCAA
GTTTGCCTTCGTCGATACAGCCTCGCGTCAGCTTCTGCCGGCGGGCTTAGCGTTCCTCGACTTCGACGAGGCGGGACCCG
AAGGTTTTGCCGCGCGCATCGTGCCGGGCGAATTCGAAACGGTGGAGGCGCAGCGGGGCGAACTTGGACAGATCCTCTAC
ACCTCCGGCTCCACCGGACGGCCGAAGGGCGTCGAGCTCAGCCACGACAGCCAACTGTGGGCGCTTGCCGCCAAGGGCGT
CAGCGCCGACACCTGCGACGAGGTCTACATCATCGCCCAGCCGCTGTTCCACATGAACGGTCTGTTCGGCGCCAAGAGCA
TCTTCGCCAGCAATGCGAGCATGGTTCTGATGCCGGGCTTCGACAGCCGGCGCTATCTGCAGGCCATGGCGGATCATGGC
GTCACCGCGGTTACTGCGGTGCCGACAATGTTCGCGCGGCTGCTGCGCGAGCCCGACCTCTTGGCCGCCAACGATTATAG
CCGGCTGAAGCGGCTGGCGCTGGCCTCTGCGCCGATCACCAGGGCCATGGCCGACAAGATTCAGGCGGCGTTTCCGCATG
CCATGCTGACTCACGGCTACGGCACCACGGAGGCCGGCCCGTCCGTGTTCGGCCCTCATCCGGAGGGCAAGCCGCTGCCG
CCGCTCACGATCGGCTATCCGATCGACCCGTCGATGGTGAAGCTGGTCGACGGCCCGAACGACAACGAGGGCGTGCTGCT
GATGCGCAATCCGGCGCTGATGACGCGCTATCGCGGCATGCCGGACAAGACCGCCTCGGTGATGCGTGACGGCTGGTACT
ACAGCGGCGATGTCATGCGTCGCGACGAGGACGGCTTCTATTATTTCATCGGCCGGGCCGACGATATGTTCGTCTGCGGC
GGCGAGAACATCTATCCCGGCGACGTCGAGAAGATGCTGGAGACGCATCCGGCAGTGCGCCAGGCCGCGGTCGTTCCTCT
CGCCGACGAGGAGCGCGGACAGATGCCGGTGGCTTTCCTCGTGTTGGCCGACGGCCAAAGTGCCACGACCGAAGACATCA
AGGCCTACGCGCTGCGCAACGGGCCGACCTATCAGCATCCGCGCCGCGTCAGCTTCGTGGCCGATCTGCCGTGGGCCGGC
ACCAACAAGATCGATCGCGCGGCGCTGATCCGCGAGGCCAAGCAACTGGAAGCCACCAATGGCTGGTCGGTTTCCTCCCA
CGCCGAAGCCGCCGCATGA

Upstream 100 bases:

>100_bases
GTCACTACGATCTCGTCGATCAGATCGAGAGCGCGGCGAGCGCGTCTGTTTCGTCGCTCAGTCGGAAAGCCGAAGAGAAG
TACAAGGTAGGAGCACGAGC

Downstream 100 bases:

>100_bases
GCGGGGCATTGGTTGGTAAGACGGCGCTGGTCACCGGCTCGAGCCGCGGCATCGGACGGGCGATCGCGATCGCTGCTGCG
CGCGAAGGCGCGGTTGTCGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSFRWKNLGDLVDRSRGLDRPAIIDLRSPEPRVWTHSEIDRLGNGVASYLTGLGLARGTTVAILSLNRAEYIAAYFGIMR
AGFVALPVNTKQPAETIDFVLRDSDTKFAFVDTASRQLLPAGLAFLDFDEAGPEGFAARIVPGEFETVEAQRGELGQILY
TSGSTGRPKGVELSHDSQLWALAAKGVSADTCDEVYIIAQPLFHMNGLFGAKSIFASNASMVLMPGFDSRRYLQAMADHG
VTAVTAVPTMFARLLREPDLLAANDYSRLKRLALASAPITRAMADKIQAAFPHAMLTHGYGTTEAGPSVFGPHPEGKPLP
PLTIGYPIDPSMVKLVDGPNDNEGVLLMRNPALMTRYRGMPDKTASVMRDGWYYSGDVMRRDEDGFYYFIGRADDMFVCG
GENIYPGDVEKMLETHPAVRQAAVVPLADEERGQMPVAFLVLADGQSATTEDIKAYALRNGPTYQHPRRVSFVADLPWAG
TNKIDRAALIREAKQLEATNGWSVSSHAEAAA

Sequences:

>Translated_512_residues
MSFRWKNLGDLVDRSRGLDRPAIIDLRSPEPRVWTHSEIDRLGNGVASYLTGLGLARGTTVAILSLNRAEYIAAYFGIMR
AGFVALPVNTKQPAETIDFVLRDSDTKFAFVDTASRQLLPAGLAFLDFDEAGPEGFAARIVPGEFETVEAQRGELGQILY
TSGSTGRPKGVELSHDSQLWALAAKGVSADTCDEVYIIAQPLFHMNGLFGAKSIFASNASMVLMPGFDSRRYLQAMADHG
VTAVTAVPTMFARLLREPDLLAANDYSRLKRLALASAPITRAMADKIQAAFPHAMLTHGYGTTEAGPSVFGPHPEGKPLP
PLTIGYPIDPSMVKLVDGPNDNEGVLLMRNPALMTRYRGMPDKTASVMRDGWYYSGDVMRRDEDGFYYFIGRADDMFVCG
GENIYPGDVEKMLETHPAVRQAAVVPLADEERGQMPVAFLVLADGQSATTEDIKAYALRNGPTYQHPRRVSFVADLPWAG
TNKIDRAALIREAKQLEATNGWSVSSHAEAAA
>Mature_511_residues
SFRWKNLGDLVDRSRGLDRPAIIDLRSPEPRVWTHSEIDRLGNGVASYLTGLGLARGTTVAILSLNRAEYIAAYFGIMRA
GFVALPVNTKQPAETIDFVLRDSDTKFAFVDTASRQLLPAGLAFLDFDEAGPEGFAARIVPGEFETVEAQRGELGQILYT
SGSTGRPKGVELSHDSQLWALAAKGVSADTCDEVYIIAQPLFHMNGLFGAKSIFASNASMVLMPGFDSRRYLQAMADHGV
TAVTAVPTMFARLLREPDLLAANDYSRLKRLALASAPITRAMADKIQAAFPHAMLTHGYGTTEAGPSVFGPHPEGKPLPP
LTIGYPIDPSMVKLVDGPNDNEGVLLMRNPALMTRYRGMPDKTASVMRDGWYYSGDVMRRDEDGFYYFIGRADDMFVCGG
ENIYPGDVEKMLETHPAVRQAAVVPLADEERGQMPVAFLVLADGQSATTEDIKAYALRNGPTYQHPRRVSFVADLPWAGT
NKIDRAALIREAKQLEATNGWSVSSHAEAAA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=26.1992619926199, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI187761345, Length=504, Percent_Identity=28.3730158730159, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI187761343, Length=504, Percent_Identity=28.3730158730159, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI42544132, Length=511, Percent_Identity=25.8317025440313, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=26.6932270916335, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI122937307, Length=501, Percent_Identity=26.5469061876248, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI58082049, Length=493, Percent_Identity=26.1663286004057, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI115511026, Length=501, Percent_Identity=25.5489021956088, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=25.5578093306288, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=25.5578093306288, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI38524616, Length=538, Percent_Identity=26.7657992565056, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI13325057, Length=366, Percent_Identity=25.9562841530055, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI8923896, Length=362, Percent_Identity=25.1381215469613, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI13375727, Length=368, Percent_Identity=26.6304347826087, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI253970489, Length=362, Percent_Identity=25.1381215469613, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI28416953, Length=382, Percent_Identity=24.3455497382199, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=550, Percent_Identity=26.9090909090909, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI145693145, Length=504, Percent_Identity=26.7857142857143, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=25.3578732106339, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788595, Length=461, Percent_Identity=27.765726681128, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=23.9382239382239, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789201, Length=380, Percent_Identity=28.1578947368421, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786801, Length=509, Percent_Identity=26.1296660117878, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790505, Length=356, Percent_Identity=25.561797752809, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786529, Length=530, Percent_Identity=23.2075471698113, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=26.5536723163842, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=26.5536723163842, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=26.5536723163842, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=355, Percent_Identity=25.9154929577465, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=375, Percent_Identity=26.1333333333333, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=509, Percent_Identity=25.147347740668, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=476, Percent_Identity=22.0588235294118, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=359, Percent_Identity=27.5766016713092, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=506, Percent_Identity=22.5296442687747, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=512, Percent_Identity=22.0703125, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=345, Percent_Identity=24.9275362318841, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=488, Percent_Identity=22.7459016393443, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=24.0223463687151, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=321, Percent_Identity=26.791277258567, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=321, Percent_Identity=26.791277258567, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=295, Percent_Identity=24.406779661017, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=477, Percent_Identity=25.3668763102725, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=551, Percent_Identity=25.5898366606171, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=371, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=520, Percent_Identity=26.1538461538462, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18859661, Length=488, Percent_Identity=27.4590163934426, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581924, Length=355, Percent_Identity=27.0422535211268, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356441, Length=503, Percent_Identity=24.0556660039761, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648257, Length=481, Percent_Identity=26.1954261954262, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21356947, Length=340, Percent_Identity=25.5882352941176, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648253, Length=492, Percent_Identity=23.5772357723577, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648255, Length=492, Percent_Identity=23.3739837398374, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583465, Length=537, Percent_Identity=23.0912476722533, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583463, Length=537, Percent_Identity=23.0912476722533, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364798, Length=537, Percent_Identity=23.0912476722533, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648260, Length=491, Percent_Identity=22.6069246435845, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21358303, Length=472, Percent_Identity=22.6694915254237, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472339, Length=378, Percent_Identity=26.1904761904762, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=26.1904761904762, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667955, Length=362, Percent_Identity=26.2430939226519, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281365686, Length=344, Percent_Identity=21.8023255813953, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653035, Length=339, Percent_Identity=23.3038348082596, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=355, Percent_Identity=22.2535211267606, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161076582, Length=346, Percent_Identity=22.8323699421965, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55780; Mature: 55649

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRWKNLGDLVDRSRGLDRPAIIDLRSPEPRVWTHSEIDRLGNGVASYLTGLGLARGTT
CCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHCCCCCCCE
VAILSLNRAEYIAAYFGIMRAGFVALPVNTKQPAETIDFVLRDSDTKFAFVDTASRQLLP
EEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHEEEECCCCEEEEEECCCCHHHH
AGLAFLDFDEAGPEGFAARIVPGEFETVEAQRGELGQILYTSGSTGRPKGVELSHDSQLW
CCEEEEECCCCCCCCCEEEECCCCCCCHHCCCCCCCEEEEECCCCCCCCCEEECCCCCEE
ALAAKGVSADTCDEVYIIAQPLFHMNGLFGAKSIFASNASMVLMPGFDSRRYLQAMADHG
EEEECCCCCCCCCCEEEEECHHHHHCCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHCC
VTAVTAVPTMFARLLREPDLLAANDYSRLKRLALASAPITRAMADKIQAAFPHAMLTHGY
CCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCC
GTTEAGPSVFGPHPEGKPLPPLTIGYPIDPSMVKLVDGPNDNEGVLLMRNPALMTRYRGM
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHCCC
PDKTASVMRDGWYYSGDVMRRDEDGFYYFIGRADDMFVCGGENIYPGDVEKMLETHPAVR
CCHHHHHHHCCCEECCCEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHCHHHH
QAAVVPLADEERGQMPVAFLVLADGQSATTEDIKAYALRNGPTYQHPRRVSFVADLPWAG
HEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHEEEECCCCCCCCCCEEEEEEECCCCC
TNKIDRAALIREAKQLEATNGWSVSSHAEAAA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFRWKNLGDLVDRSRGLDRPAIIDLRSPEPRVWTHSEIDRLGNGVASYLTGLGLARGTT
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHCCCCCCCE
VAILSLNRAEYIAAYFGIMRAGFVALPVNTKQPAETIDFVLRDSDTKFAFVDTASRQLLP
EEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHEEEECCCCEEEEEECCCCHHHH
AGLAFLDFDEAGPEGFAARIVPGEFETVEAQRGELGQILYTSGSTGRPKGVELSHDSQLW
CCEEEEECCCCCCCCCEEEECCCCCCCHHCCCCCCCEEEEECCCCCCCCCEEECCCCCEE
ALAAKGVSADTCDEVYIIAQPLFHMNGLFGAKSIFASNASMVLMPGFDSRRYLQAMADHG
EEEECCCCCCCCCCEEEEECHHHHHCCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHCC
VTAVTAVPTMFARLLREPDLLAANDYSRLKRLALASAPITRAMADKIQAAFPHAMLTHGY
CCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCC
GTTEAGPSVFGPHPEGKPLPPLTIGYPIDPSMVKLVDGPNDNEGVLLMRNPALMTRYRGM
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHCCC
PDKTASVMRDGWYYSGDVMRRDEDGFYYFIGRADDMFVCGGENIYPGDVEKMLETHPAVR
CCHHHHHHHCCCEECCCEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHCHHHH
QAAVVPLADEERGQMPVAFLVLADGQSATTEDIKAYALRNGPTYQHPRRVSFVADLPWAG
HEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHEEEECCCCCCCCCCEEEEEEECCCCC
TNKIDRAALIREAKQLEATNGWSVSSHAEAAA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]