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The map label for this gene is xthA [H]

Identifier: 86750015

GI number: 86750015

Start: 3299593

End: 3300447

Strand: Reverse

Name: xthA [H]

Synonym: RPB_2898

Alternate gene names: 86750015

Gene position: 3300447-3299593 (Counterclockwise)

Preceding gene: 86750016

Following gene: 86750012

Centisome position: 61.9

GC content: 67.72

Gene sequence:

>855_bases
ATGATGGCCTCCTGCAATGAACAGCCATGCAACTGCCGCCCGACGCACGCGTTCCCTCGTGGAGCGAGCGAGGAAGCGCC
CCTGAAGATCGCCACCTTCAACGTCAACAACGTCAACAAGCGGCTGCCGGTGCTGCTCGGCTGGCTGAAGGCGGCGAAGC
CGGACATCGTCTGCCTGCAGGAGCTGAAGGCGGCGCAGGACGACTTTCCGGAAGCGGCGCTGCTCAAGGCTGGCTATTCC
GCCGCCTGGGTCGGACAGAAGACCTGGAACGGTGTCGCGATTCTCGCCCGCAAGCGCGCCGAGATCGTGGTCACCCGCCG
GGCCTTGCCGGGCGACGCCAAGGATCTGCAGAGCCGCTATCTGGAAGCCGCGGTCGCCGGGATGATCGTCGGCTGCCTGT
ACGCGCCGAACGGCAATCCACAGCCCGGGCCGAAATTCCAGTACAAGCTGGCCTGGATGAAACGGCTCAACCGGCATGCG
GCCAAGCTGCTGAAGAGCGGTGTGCCGGTGGTGCTGGCCGGCGATTTCAACGTGGTGCCGACGCCGGCCGACATCTACCC
GACGCGGTCGTGGGACAAGGATGCGCTGCTGCAGCCGGAGCCGCGGGCGCTGTTCGCCGCCTTGCTGAAGCAGGGCTGGA
CTGACGCGCTGCGCGCGCTGCATCCCGAGCAGGCGCCGTACACGTTCTGGACCTACTGGCGAAACCGGTTCGAGCGCGAC
CACGGGCTGCGGATCGATCATTTGCTGCTCGACCCGGCCAGTGCGCAGCGGCTGGCGCGCGGCGGCGTCGACCGCGCGAT
CCGCGGCGCTCCGGACGCCAGCGACCATGCGCCGGCCTGGATCGTGCTGAAATAG

Upstream 100 bases:

>100_bases
GTCGCCGGTGCCGGGCGCCTGCTGCTGGCCCTTGGTCGCCGCCGGATCGACGGTGGCCGCGCGGCCCGCGATCGGGGTGG
GCTGATGGTCGTCTTTGGAC

Downstream 100 bases:

>100_bases
AGCGGCGCGCCGGTCCGCCGCGACGGCAGGAGAGGCGGGGATCGCAGCCTCGGCGCATTGGTCCGGCGCGAGGCTGTTGC
CTCTCCGGCTCCGGGCCTAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MMASCNEQPCNCRPTHAFPRGASEEAPLKIATFNVNNVNKRLPVLLGWLKAAKPDIVCLQELKAAQDDFPEAALLKAGYS
AAWVGQKTWNGVAILARKRAEIVVTRRALPGDAKDLQSRYLEAAVAGMIVGCLYAPNGNPQPGPKFQYKLAWMKRLNRHA
AKLLKSGVPVVLAGDFNVVPTPADIYPTRSWDKDALLQPEPRALFAALLKQGWTDALRALHPEQAPYTFWTYWRNRFERD
HGLRIDHLLLDPASAQRLARGGVDRAIRGAPDASDHAPAWIVLK

Sequences:

>Translated_284_residues
MMASCNEQPCNCRPTHAFPRGASEEAPLKIATFNVNNVNKRLPVLLGWLKAAKPDIVCLQELKAAQDDFPEAALLKAGYS
AAWVGQKTWNGVAILARKRAEIVVTRRALPGDAKDLQSRYLEAAVAGMIVGCLYAPNGNPQPGPKFQYKLAWMKRLNRHA
AKLLKSGVPVVLAGDFNVVPTPADIYPTRSWDKDALLQPEPRALFAALLKQGWTDALRALHPEQAPYTFWTYWRNRFERD
HGLRIDHLLLDPASAQRLARGGVDRAIRGAPDASDHAPAWIVLK
>Mature_284_residues
MMASCNEQPCNCRPTHAFPRGASEEAPLKIATFNVNNVNKRLPVLLGWLKAAKPDIVCLQELKAAQDDFPEAALLKAGYS
AAWVGQKTWNGVAILARKRAEIVVTRRALPGDAKDLQSRYLEAAVAGMIVGCLYAPNGNPQPGPKFQYKLAWMKRLNRHA
AKLLKSGVPVVLAGDFNVVPTPADIYPTRSWDKDALLQPEPRALFAALLKQGWTDALRALHPEQAPYTFWTYWRNRFERD
HGLRIDHLLLDPASAQRLARGGVDRAIRGAPDASDHAPAWIVLK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI18375503, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI18375501, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=31.2267657992565, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71989536, Length=234, Percent_Identity=26.0683760683761, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330655, Length=282, Percent_Identity=30.8510638297872, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17136678, Length=282, Percent_Identity=30.8510638297872, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31439; Mature: 31439

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMASCNEQPCNCRPTHAFPRGASEEAPLKIATFNVNNVNKRLPVLLGWLKAAKPDIVCLQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHH
ELKAAQDDFPEAALLKAGYSAAWVGQKTWNGVAILARKRAEIVVTRRALPGDAKDLQSRY
HHHHHHCCCCCHHHHHCCCCEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHH
LEAAVAGMIVGCLYAPNGNPQPGPKFQYKLAWMKRLNRHAAKLLKSGVPVVLAGDFNVVP
HHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECC
TPADIYPTRSWDKDALLQPEPRALFAALLKQGWTDALRALHPEQAPYTFWTYWRNRFERD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
HGLRIDHLLLDPASAQRLARGGVDRAIRGAPDASDHAPAWIVLK
CCCEEEEEEECCHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MMASCNEQPCNCRPTHAFPRGASEEAPLKIATFNVNNVNKRLPVLLGWLKAAKPDIVCLQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHH
ELKAAQDDFPEAALLKAGYSAAWVGQKTWNGVAILARKRAEIVVTRRALPGDAKDLQSRY
HHHHHHCCCCCHHHHHCCCCEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHH
LEAAVAGMIVGCLYAPNGNPQPGPKFQYKLAWMKRLNRHAAKLLKSGVPVVLAGDFNVVP
HHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECC
TPADIYPTRSWDKDALLQPEPRALFAALLKQGWTDALRALHPEQAPYTFWTYWRNRFERD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
HGLRIDHLLLDPASAQRLARGGVDRAIRGAPDASDHAPAWIVLK
CCCEEEEEEECCHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]