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The map label for this gene is lipA

Identifier: 86750005

GI number: 86750005

Start: 3288171

End: 3289130

Strand: Reverse

Name: lipA

Synonym: RPB_2888

Alternate gene names: 86750005

Gene position: 3289130-3288171 (Counterclockwise)

Preceding gene: 86750007

Following gene: 86750004

Centisome position: 61.69

GC content: 67.6

Gene sequence:

>960_bases
ATGGTCGTCCTCGTCGATACGGTCTCGTCCACCCCCGTCCGCCCCCGGCATCCGGAGAAGGCGGCGCGGCCGGACGCGCT
GTCGCCGAAGAAGCCGGACTGGATCCGCGTCCGCGCGCCGACCACCCGCGGCTATGGCGAGACCCGCTCGATCGTCAAGG
AGAACGGCCTCGTCACGGTGTGCGAGGAGGCCGGCTGCCCGAATATCGGCGAGTGCTGGGACAAGAAGCACGCCACCTTC
ATGATCATGGGGGACACCTGCACCCGGGCCTGCGCGTTCTGCAACGTCAAGACCGGGATGCCCGGCGCGCTCGATCCGAA
CGAGCCGGCCTATGTGGCTGAAGCCACCCGCAAGCTCGGGCTCGAACATCTGGTGATCACCTCGGTCGACCGCGACGATC
TCGCCGACGGCGGCGCCGCGCATTTCGCCGCGACCATCCGCGCGGTCCGCGAGGCGTGCCCGACCACCACGATCGAAATT
CTCACGCCGGACTTTTTGCGCAAGGACGGCGCGCTCGAGGTCGTGGTCGCCGCCAAGCCGGACGTGTTCAACCACAATCT
CGAAACCGTGCCGTCGCGCTACCTGTCGGTGCGGCCGGGCGCGCGCTACTTCCATTCGATCCGGCTGTTGCAGCGGGTGA
AGGAGCTGGACCCGACGCTGTTCACCAAATCCGGCATCATGGTCGGGCTCGGCGAGGAGCGCCACGAGGTGTTGCAGGTG
ATGGACGATCTGCGCTCCGCCGAGGTCGATTTCCTCACCATCGGGCAATATCTGCAGCCGACCCGCAAGCATCACGCGGT
GATGCGCTACGTCACGCCGGACGAGTTCGGCGGCTACGCCAAGACGGCTTACGCCAAGGGCTTCCTGATGGTGTCGGCGA
GCCCGATGACGCGCTCTTCGCATCACGCCGGCGACGATTTCGCCAAGCTGCGGGCAGCGCGCGCGGCGCTGGCGCGCTGA

Upstream 100 bases:

>100_bases
ATCGAGCATTATTCCAAGGGATAAGCGCCCGCAAGCAGCCCGGGTTGCGGGACGGGGGCGCTCGGATTAGGTAGGGTTCT
CGCTCACCTTCAGGCAGCCC

Downstream 100 bases:

>100_bases
GTTCAGGACGCCGGGTTCGCAACGATGCCGCAATTTTCCAGCAAGCGCCGGGTGCCGCACAGCGCCGAACAGATGTTCGA
TCTGGTCGCCGACGTCGAGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVVLVDTVSSTPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRSIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGALDPNEPAYVAEATRKLGLEHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEI
LTPDFLRKDGALEVVVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPTLFTKSGIMVGLGEERHEVLQV
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFGGYAKTAYAKGFLMVSASPMTRSSHHAGDDFAKLRAARAALAR

Sequences:

>Translated_319_residues
MVVLVDTVSSTPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRSIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGALDPNEPAYVAEATRKLGLEHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEI
LTPDFLRKDGALEVVVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPTLFTKSGIMVGLGEERHEVLQV
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFGGYAKTAYAKGFLMVSASPMTRSSHHAGDDFAKLRAARAALAR
>Mature_319_residues
MVVLVDTVSSTPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRSIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGALDPNEPAYVAEATRKLGLEHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEI
LTPDFLRKDGALEVVVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPTLFTKSGIMVGLGEERHEVLQV
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFGGYAKTAYAKGFLMVSASPMTRSSHHAGDDFAKLRAARAALAR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=44.1379310344828, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=46.0905349794239, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=52.3465703971119, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.7364341085271, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=44.2567567567568, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=43.4628975265018, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_RHOP2 (Q2IW20)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486501.1
- ProteinModelPortal:   Q2IW20
- STRING:   Q2IW20
- GeneID:   3910682
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2888
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   RPAL316058:RPB_2888-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35086; Mature: 35086

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVLVDTVSSTPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRSIVKENGLVTV
CEEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGALDPNEPAYVAEATRKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHCC
LEHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEILTPDFLRKDGALEVVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEECCC
DVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPTLFTKSGIMVGLGEERHEVLQV
CHHCCCHHHHCHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHH
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFGGYAKTAYAKGFLMVSASPMTRSS
HHHHHHCCCCEEEHHHHHCHHHHHHHEEEEECCHHCCCCHHHHHHCEEEEEECCCCCCCC
HHAGDDFAKLRAARAALAR
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVLVDTVSSTPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRSIVKENGLVTV
CEEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGALDPNEPAYVAEATRKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHCC
LEHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEILTPDFLRKDGALEVVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEECCC
DVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPTLFTKSGIMVGLGEERHEVLQV
CHHCCCHHHHCHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHH
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFGGYAKTAYAKGFLMVSASPMTRSS
HHHHHHCCCCEEEHHHHHCHHHHHHHEEEEECCHHCCCCHHHHHHCEEEEEECCCCCCCC
HHAGDDFAKLRAARAALAR
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA