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The map label for this gene is glnS [H]

Identifier: 86749928

GI number: 86749928

Start: 3202762

End: 3204447

Strand: Reverse

Name: glnS [H]

Synonym: RPB_2811

Alternate gene names: 86749928

Gene position: 3204447-3202762 (Counterclockwise)

Preceding gene: 86749931

Following gene: 86749927

Centisome position: 60.1

GC content: 63.82

Gene sequence:

>1686_bases
ATGACCACGGCAGACGCAACGACCTCCGAGGCAGGGCGCGACTTCATTCGCGATATCGTCCAGGCCGATCTCGAGGCCGG
CCGGCACGCCCAGGTCGTGACCCGGTTCCCGCCTGAGCCGAACGGGTATCTGCATATCGGCCACGCCAAGTCGATCGCGC
TGAATTTCGGCATCGCCCAGGAATTCGGCGGCCGCTGCAATTTGCGGTTCGACGACACCAATCCGACCAGGGAAGAGCAG
GAATATATCGATTCGATTCAGGCCGACGTGCGCTGGCTCGGCTTCGACTGGGGCGAGCACATGTTCTTCGCCTCGGATTA
TTTCGACCGGCTCTACGATTGGGCCGAGCTGTTGATCAAGAACGGCGACGCCTATGTCGACGACCAGTCGCAGGATGAAA
TCCGCGAGGCGCGCGGTACGCTGATGGAGCCCGGCCGGAACAGCCCGTTCCGCGATCGCTCAGTGGAAGACAATCTCGAT
CTGTTCCGCCGCATGAAGGCCGGCGAGTTTCCGAACGGCGCGCGCGTGCTGCGCGCCAAGATCGACATGGCGTCGGGCAA
CATCAATCTGCGCGACCCGGTGCTGTACCGCATCCTGCATGCGCATCACCCGCGCACCGGCGACGCCTGGTCGATCTACC
CGAGCTACGACTACGCGCACGGCCAGTCGGATGCGATCGAGGGCGTCACGCATTCGATCTGTACACTCGAGTTCGAGGAT
CACCGGCCGCTGTACGAATGGTTGCTGTCGAAGCTGCCGGTGCCGTCGCAGCCGAAGCAGTACGAGTTCGCGCGGCTCAA
CATCACCTACACGCTGCTGTCGAAACGTGTGCTGACCGAACTCGTTCGCGGCGGCCATGTCGCCGGCTGGGACGATCCGC
GGATGCCGACGATGGCCGGCTTGAAGCGTCGCGGCGTGCCACCGGCGGCGATCCGCGAGTTCGTCAAGCGGATCGGGGTG
GCCAAGGCCAACAGCGTGGTCGATGTCGGTATGCTGGAATTCTGCATCCGCGAGGAGCTGAACCGCACCTCGCAGCGCCG
GATGGCGGTACTGCGGCCGCTGAAAGTGGTGATCGAGAACTATCCGGAAGGGCAGACCGAAGAGCTCGACGCGATCAACC
ATCCCGACGATCCCTCCGCCGGCACCCGCAAGATCGCGTTCGGCCGCGAGCTTTATATCGAGCAGGACGATTTCATGGAG
AACCCGCCGAAGAAGTTCTTCCGGCTCTCTCCCGGCAACGAAGTCCGGCTGCGCTACGCTTACTTCATCAAGTGCCGCGA
GGTGGTGAAGAACGATGCCGGCGAGATCGTCGAGCTGCGCTGCACCTACGATCCCGAAACCCGCGGCGGCAACGCGCCGG
ACGGCCGCAAGGTCAAGGCGACGATGCACTGGCTGTCGGCGGCACAATCGGTGCCGGCGGAAATCCGGGTCTACAATCAG
TTGTTCGCCAATCCGGCGCCGAGTGCGGCGAATTTCACCGCCGACCTCAATCCGCAGTCGCTCGAAGTGCTGACCTGCGC
CCGGATCGAGCCGGCGGTCGCGGCCGGCGTTTCGGCCGAGCCGATCCAGTTCGAGCGCCAGGGTTACTTCGTCCGCGACA
AGGACTCGACCGCGGACAAGCCGGTGTTCAACCGCACCATCGGGCTGCGTGATACCTTCGCCAAGGAAGTCGGCGGTAAA
AGCTGA

Upstream 100 bases:

>100_bases
CGCATAACTAAAGTGGTCCGCGCAGCCTTCGGGGGTTGGCGCGGGCCCCCGGATTTGGCAGATACCGCCTCGGGTTCCGA
TACGGCAGAAAGATCAATTC

Downstream 100 bases:

>100_bases
CGCTTCCGCCGGCGATTGTTGCCGGCGATGCTGATCTGCTCCAGTCTTGGCGCCGCGCTGTTTGAGGGGCGGGCGCCGTG
GAGCGGGGCGATCGAGCCAG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTTADATTSEAGRDFIRDIVQADLEAGRHAQVVTRFPPEPNGYLHIGHAKSIALNFGIAQEFGGRCNLRFDDTNPTREEQ
EYIDSIQADVRWLGFDWGEHMFFASDYFDRLYDWAELLIKNGDAYVDDQSQDEIREARGTLMEPGRNSPFRDRSVEDNLD
LFRRMKAGEFPNGARVLRAKIDMASGNINLRDPVLYRILHAHHPRTGDAWSIYPSYDYAHGQSDAIEGVTHSICTLEFED
HRPLYEWLLSKLPVPSQPKQYEFARLNITYTLLSKRVLTELVRGGHVAGWDDPRMPTMAGLKRRGVPPAAIREFVKRIGV
AKANSVVDVGMLEFCIREELNRTSQRRMAVLRPLKVVIENYPEGQTEELDAINHPDDPSAGTRKIAFGRELYIEQDDFME
NPPKKFFRLSPGNEVRLRYAYFIKCREVVKNDAGEIVELRCTYDPETRGGNAPDGRKVKATMHWLSAAQSVPAEIRVYNQ
LFANPAPSAANFTADLNPQSLEVLTCARIEPAVAAGVSAEPIQFERQGYFVRDKDSTADKPVFNRTIGLRDTFAKEVGGK
S

Sequences:

>Translated_561_residues
MTTADATTSEAGRDFIRDIVQADLEAGRHAQVVTRFPPEPNGYLHIGHAKSIALNFGIAQEFGGRCNLRFDDTNPTREEQ
EYIDSIQADVRWLGFDWGEHMFFASDYFDRLYDWAELLIKNGDAYVDDQSQDEIREARGTLMEPGRNSPFRDRSVEDNLD
LFRRMKAGEFPNGARVLRAKIDMASGNINLRDPVLYRILHAHHPRTGDAWSIYPSYDYAHGQSDAIEGVTHSICTLEFED
HRPLYEWLLSKLPVPSQPKQYEFARLNITYTLLSKRVLTELVRGGHVAGWDDPRMPTMAGLKRRGVPPAAIREFVKRIGV
AKANSVVDVGMLEFCIREELNRTSQRRMAVLRPLKVVIENYPEGQTEELDAINHPDDPSAGTRKIAFGRELYIEQDDFME
NPPKKFFRLSPGNEVRLRYAYFIKCREVVKNDAGEIVELRCTYDPETRGGNAPDGRKVKATMHWLSAAQSVPAEIRVYNQ
LFANPAPSAANFTADLNPQSLEVLTCARIEPAVAAGVSAEPIQFERQGYFVRDKDSTADKPVFNRTIGLRDTFAKEVGGK
S
>Mature_560_residues
TTADATTSEAGRDFIRDIVQADLEAGRHAQVVTRFPPEPNGYLHIGHAKSIALNFGIAQEFGGRCNLRFDDTNPTREEQE
YIDSIQADVRWLGFDWGEHMFFASDYFDRLYDWAELLIKNGDAYVDDQSQDEIREARGTLMEPGRNSPFRDRSVEDNLDL
FRRMKAGEFPNGARVLRAKIDMASGNINLRDPVLYRILHAHHPRTGDAWSIYPSYDYAHGQSDAIEGVTHSICTLEFEDH
RPLYEWLLSKLPVPSQPKQYEFARLNITYTLLSKRVLTELVRGGHVAGWDDPRMPTMAGLKRRGVPPAAIREFVKRIGVA
KANSVVDVGMLEFCIREELNRTSQRRMAVLRPLKVVIENYPEGQTEELDAINHPDDPSAGTRKIAFGRELYIEQDDFMEN
PPKKFFRLSPGNEVRLRYAYFIKCREVVKNDAGEIVELRCTYDPETRGGNAPDGRKVKATMHWLSAAQSVPAEIRVYNQL
FANPAPSAANFTADLNPQSLEVLTCARIEPAVAAGVSAEPIQFERQGYFVRDKDSTADKPVFNRTIGLRDTFAKEVGGKS

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=528, Percent_Identity=43.5606060606061, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI62241042, Length=518, Percent_Identity=32.2393822393822, Blast_Score=262, Evalue=8e-70,
Organism=Escherichia coli, GI1786895, Length=544, Percent_Identity=55.5147058823529, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=124, Percent_Identity=29.8387096774194, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543332, Length=535, Percent_Identity=40.5607476635514, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=522, Percent_Identity=31.4176245210728, Blast_Score=228, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6324742, Length=566, Percent_Identity=41.5194346289753, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321192, Length=532, Percent_Identity=34.7744360902256, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI17864488, Length=533, Percent_Identity=42.5891181988743, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24649466, Length=375, Percent_Identity=37.3333333333333, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63506; Mature: 63375

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTADATTSEAGRDFIRDIVQADLEAGRHAQVVTRFPPEPNGYLHIGHAKSIALNFGIAQ
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEECCHHH
EFGGRCNLRFDDTNPTREEQEYIDSIQADVRWLGFDWGEHMFFASDYFDRLYDWAELLIK
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
NGDAYVDDQSQDEIREARGTLMEPGRNSPFRDRSVEDNLDLFRRMKAGEFPNGARVLRAK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
IDMASGNINLRDPVLYRILHAHHPRTGDAWSIYPSYDYAHGQSDAIEGVTHSICTLEFED
EEECCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHEEEEEEECC
HRPLYEWLLSKLPVPSQPKQYEFARLNITYTLLSKRVLTELVRGGHVAGWDDPRMPTMAG
CCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LKRRGVPPAAIREFVKRIGVAKANSVVDVGMLEFCIREELNRTSQRRMAVLRPLKVVIEN
HHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPEGQTEELDAINHPDDPSAGTRKIAFGRELYIEQDDFMENPPKKFFRLSPGNEVRLRYA
CCCCCCHHHCCCCCCCCCCCCCEEEECCCEEEEECHHHCCCCCHHHEECCCCCCEEEEHH
YFIKCREVVKNDAGEIVELRCTYDPETRGGNAPDGRKVKATMHWLSAAQSVPAEIRVYNQ
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHH
LFANPAPSAANFTADLNPQSLEVLTCARIEPAVAAGVSAEPIQFERQGYFVRDKDSTADK
HHCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHCCCCCCCCEECCCCCEEECCCCCCCC
PVFNRTIGLRDTFAKEVGGKS
CHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
TTADATTSEAGRDFIRDIVQADLEAGRHAQVVTRFPPEPNGYLHIGHAKSIALNFGIAQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEECCHHH
EFGGRCNLRFDDTNPTREEQEYIDSIQADVRWLGFDWGEHMFFASDYFDRLYDWAELLIK
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
NGDAYVDDQSQDEIREARGTLMEPGRNSPFRDRSVEDNLDLFRRMKAGEFPNGARVLRAK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
IDMASGNINLRDPVLYRILHAHHPRTGDAWSIYPSYDYAHGQSDAIEGVTHSICTLEFED
EEECCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHEEEEEEECC
HRPLYEWLLSKLPVPSQPKQYEFARLNITYTLLSKRVLTELVRGGHVAGWDDPRMPTMAG
CCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LKRRGVPPAAIREFVKRIGVAKANSVVDVGMLEFCIREELNRTSQRRMAVLRPLKVVIEN
HHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPEGQTEELDAINHPDDPSAGTRKIAFGRELYIEQDDFMENPPKKFFRLSPGNEVRLRYA
CCCCCCHHHCCCCCCCCCCCCCEEEECCCEEEEECHHHCCCCCHHHEECCCCCCEEEEHH
YFIKCREVVKNDAGEIVELRCTYDPETRGGNAPDGRKVKATMHWLSAAQSVPAEIRVYNQ
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHH
LFANPAPSAANFTADLNPQSLEVLTCARIEPAVAAGVSAEPIQFERQGYFVRDKDSTADK
HHCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHCCCCCCCCEECCCCCEEECCCCCCCC
PVFNRTIGLRDTFAKEVGGKS
CHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]