Click here to switch to the map view.

The map label for this gene is tpiA [H]

Identifier: 86749912

GI number: 86749912

Start: 3185514

End: 3186299

Strand: Reverse

Name: tpiA [H]

Synonym: RPB_2795

Alternate gene names: 86749912

Gene position: 3186299-3185514 (Counterclockwise)

Preceding gene: 86749918

Following gene: 86749911

Centisome position: 59.76

GC content: 66.79

Gene sequence:

>786_bases
ATGAGAGTGTCCATGCCGGTCCCCGCCGTTCGTTCGCTGATTGCCGGAAACTGGAAGATGAACGGCCTCAAGGCCGCTTC
GTCTGAACTCGATGCGATCCTGGAAGGCGCGGCGCAACTTTCGTCGGGGGTCGATCTGCTGGTATGCCCGCCCGCGACGC
TGATTGCGGCGTTCGCCGATCGGCTCGCGGCCCACGCCAACAGCGCGCTGCCGGTCGCAATTGGTGCACAGGATTGCCAC
ACCAATGCCGCAGGCGCACATACCGGCGACATCTCGGCCGAAATGCTGGCGGATGCCGGCGCCCGCACTATTATCGTCGG
CCATTCCGAGCGCCGCGCCGACCACGCCGAGACCGACGCGATCGTCCGCACCAAGGCGCAGGCGGTCTGGCGGGCAGGGT
TGGTTGCGATCGTCTGCGTTGGCGAAACCCAAGGTGAGCGCAATGCCGGCCACACCCTCGATGTGGTCGCCGGCCAGCTC
GCCGGCTCGCTGCCGGACGGCGCAACCGCCGCCAATCTGGTGGTGGCCTATGAGCCGGTCTGGGCGATCGGCACCGGCAT
GACGCCGACTGCCGCAGATGTCGAGGAAGTTCATGGATTTATCCGCCAGACTCTGACGCAACGCTTCAACGGCGAGGGCG
AGCGCATGCGGTTGCTTTACGGCGGTTCGGTGAAGCCCTCCAACGCCAGCGAGCTGATGGCGGTTCCCCACGTCAACGGC
GCGCTGGTCGGTGGCGCCAGCCTGAAGGCGTCGGACTTCCTTGCGATCGCGGCCGGGTGCCCCTGA

Upstream 100 bases:

>100_bases
GCGCGTCGCAACAGGCTCTGGCTGCGGTCCGCCCCTGTGCGATCACGCGCTCTTGAGCATCGCAAACGGCTCTGCTAGCG
GGATTGAGCAACCCCGAAAC

Downstream 100 bases:

>100_bases
GCATCCGGGGCGGAAGTTACGGCAGGACGATCACGAAGGCCGAGAGGAAATTCCGCCGGATCTGCGGGCTCTGCTCTTGC
CAACAATTCGCCACGCGGTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRVSMPVPAVRSLIAGNWKMNGLKAASSELDAILEGAAQLSSGVDLLVCPPATLIAAFADRLAAHANSALPVAIGAQDCH
TNAAGAHTGDISAEMLADAGARTIIVGHSERRADHAETDAIVRTKAQAVWRAGLVAIVCVGETQGERNAGHTLDVVAGQL
AGSLPDGATAANLVVAYEPVWAIGTGMTPTAADVEEVHGFIRQTLTQRFNGEGERMRLLYGGSVKPSNASELMAVPHVNG
ALVGGASLKASDFLAIAAGCP

Sequences:

>Translated_261_residues
MRVSMPVPAVRSLIAGNWKMNGLKAASSELDAILEGAAQLSSGVDLLVCPPATLIAAFADRLAAHANSALPVAIGAQDCH
TNAAGAHTGDISAEMLADAGARTIIVGHSERRADHAETDAIVRTKAQAVWRAGLVAIVCVGETQGERNAGHTLDVVAGQL
AGSLPDGATAANLVVAYEPVWAIGTGMTPTAADVEEVHGFIRQTLTQRFNGEGERMRLLYGGSVKPSNASELMAVPHVNG
ALVGGASLKASDFLAIAAGCP
>Mature_261_residues
MRVSMPVPAVRSLIAGNWKMNGLKAASSELDAILEGAAQLSSGVDLLVCPPATLIAAFADRLAAHANSALPVAIGAQDCH
TNAAGAHTGDISAEMLADAGARTIIVGHSERRADHAETDAIVRTKAQAVWRAGLVAIVCVGETQGERNAGHTLDVVAGQL
AGSLPDGATAANLVVAYEPVWAIGTGMTPTAADVEEVHGFIRQTLTQRFNGEGERMRLLYGGSVKPSNASELMAVPHVNG
ALVGGASLKASDFLAIAAGCP

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=38, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=38, Blast_Score=154, Evalue=6e-38,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=41.869918699187, Blast_Score=177, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=42.1052631578947, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320255, Length=238, Percent_Identity=39.9159663865546, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=42.8, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=42.8, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=42.8, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26629; Mature: 26629

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSMPVPAVRSLIAGNWKMNGLKAASSELDAILEGAAQLSSGVDLLVCPPATLIAAFAD
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHH
RLAAHANSALPVAIGAQDCHTNAAGAHTGDISAEMLADAGARTIIVGHSERRADHAETDA
HHHHHCCCCCEEEECCHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCHHCCCHHHHHH
IVRTKAQAVWRAGLVAIVCVGETQGERNAGHTLDVVAGQLAGSLPDGATAANLVVAYEPV
HHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
WAIGTGMTPTAADVEEVHGFIRQTLTQRFNGEGERMRLLYGGSVKPSNASELMAVPHVNG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCC
ALVGGASLKASDFLAIAAGCP
EEECCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MRVSMPVPAVRSLIAGNWKMNGLKAASSELDAILEGAAQLSSGVDLLVCPPATLIAAFAD
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHH
RLAAHANSALPVAIGAQDCHTNAAGAHTGDISAEMLADAGARTIIVGHSERRADHAETDA
HHHHHCCCCCEEEECCHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCHHCCCHHHHHH
IVRTKAQAVWRAGLVAIVCVGETQGERNAGHTLDVVAGQLAGSLPDGATAANLVVAYEPV
HHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
WAIGTGMTPTAADVEEVHGFIRQTLTQRFNGEGERMRLLYGGSVKPSNASELMAVPHVNG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCC
ALVGGASLKASDFLAIAAGCP
EEECCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA