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The map label for this gene is pyrG [H]

Identifier: 86749907

GI number: 86749907

Start: 3179030

End: 3180661

Strand: Reverse

Name: pyrG [H]

Synonym: RPB_2790

Alternate gene names: 86749907

Gene position: 3180661-3179030 (Counterclockwise)

Preceding gene: 86749908

Following gene: 86749903

Centisome position: 59.66

GC content: 64.46

Gene sequence:

>1632_bases
ATGGCGCGGTATATTTTCATCACCGGCGGCGTGGTCTCCTCGCTCGGCAAGGGTCTCGCATCAGCGGCACTCGGCGCGCT
GCTGCAGGCGCGAGGCTACAAGGTCCGCCTTCGCAAGCTCGATCCCTATCTCAATCTCGATCCCGGGACGATGTCGCCGT
ATCAGCACGGCGAAGTGTTCGTGACCGACGACGGCGCCGAGACCGATCTCGATCTCGGCCATTACGAACGGTTCACCGGG
CGGCCCGCCACCCGGCAGGACAACATCACCACCGGTCGGATCTATCAGGACATCCTGACCAAGGAACGCCGCGGCGACTA
TCTCGGTGCCACCATCCAGGTGGTCCCGCACGTCACCAACGCGATCAAGGACTTCATCGTCGATTCCAACGACGGCTACG
ATTTCGTGCTGGTCGAGATCGGCGGCACCGTCGGCGACATCGAAGGCCTGCCGTTCTTCGAGGCGATCCGACAGATCAAG
AACGATCTGCCGCGCGGCGACGTGATCTACATTCACCTGACGCTGCTGCCCTACATCCCGAGCGCCGGCGAATTGAAGAC
CAAGCCGACCCAGCATTCGGTCAAGGAGCTGCGTTCGATCGGCATCCAGCCCGACATCCTGCTGTGCCGCACCGACCGTC
CGATCCCGAAAGAGGAACGGCGCAAGCTCGGGCTGTTCTGCAACGTCCGCGAAAGCGCGGTGATCGAGGCGCGCGACGCC
GACAGCATTTATGCTGTGCCGGAGGCGTATCACGCCGCAGGCCTCGACGACGAAGTGCTGGCGGCATTTGCGATTGCGGC
CGAACAGCCGCCGGCGCTGGAGCGCTGGCATCAGATCAACGAGCGTATCCGCAATCCTGAAGGCGCGGTGACGATCGCCA
TCGTCGGCAAATACACCGGGATGAAGGACGCCTACAAGTCGCTGATCGAGGCGCTGTCGCACGGCGGCATCGCCAACAAG
GTCCAGGTCAAGCTCGACTGGATCGAGAGCGAAGTGTTCGAGAACGAGGACCCGGCGCCGTTCCTCGAGCACGTCAACGG
AATCCTGGTGCCCGGCGGCTTCGGTCAGCGCGGCGCCGAGGGCAAGATCAAGGCGGCGCAGTTCGCCCGCGAACGCGACG
TGCCGTATTTCGGCATCTGCTTCGGCATGCAGATGGCGGTGATCGAGGCGGCGCGCAATCTCGCCGGCATCGAGCAAGCC
AACTCGACCGAATTCGGCCCGACGGCGGAGCCGCTGGTCGGCCTGATGACGGAATGGCTGCGCGGCAACGAGTTGGAGCG
GCGCTCCAACGCCGGCGACCTCGGCGGGACGATGCGGCTCGGCGCCTATCCGGCGGCGCTGAAGCGCGGCAGCCGGGTGT
CGAAGATCTACGGCGACGTGCTGGAGATTTCAGAGCGCCACCGCCATCGCTACGAGGTCAACACCGCCTACAAGGACCGG
CTCGAACAGCACGGCCTGCGCTTCTCCGGCATGTCACCGGACGGTGTGCTGCCGGAGATCGTCGAATACGAGGATCATCC
GTGGTTCATCGGGGTGCAGTTCCACCCCGAACTGAAATCACGCCCGTTCGAGCCGCATCCGCTGTTCTCGTCCTTCATCG
AAGCGGCGCTGGTGCGCAGCCGGCTGGTGTAG

Upstream 100 bases:

>100_bases
GCGCTGATCTTTCAGCGTCACCCACAGGCATTTCGAAGTTCGCCCATTAGGGACGAATCGCTTTGGCGAATCGGACCGAG
GGCCTTAAAGGTAGGGTCCC

Downstream 100 bases:

>100_bases
TTTCCATCACTTCGGGCAGCCCGCCGTGGAATCGCCCGCGCGTGCCATCGGCTTGCCGTTGATGAAGACGTTGCTGCCGC
CGCCGACCACCACGCCGCCG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPATRQDNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFIVDSNDGYDFVLVEIGGTVGDIEGLPFFEAIRQIK
NDLPRGDVIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDA
DSIYAVPEAYHAAGLDDEVLAAFAIAAEQPPALERWHQINERIRNPEGAVTIAIVGKYTGMKDAYKSLIEALSHGGIANK
VQVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIKAAQFARERDVPYFGICFGMQMAVIEAARNLAGIEQA
NSTEFGPTAEPLVGLMTEWLRGNELERRSNAGDLGGTMRLGAYPAALKRGSRVSKIYGDVLEISERHRHRYEVNTAYKDR
LEQHGLRFSGMSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFSSFIEAALVRSRLV

Sequences:

>Translated_543_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPATRQDNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFIVDSNDGYDFVLVEIGGTVGDIEGLPFFEAIRQIK
NDLPRGDVIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDA
DSIYAVPEAYHAAGLDDEVLAAFAIAAEQPPALERWHQINERIRNPEGAVTIAIVGKYTGMKDAYKSLIEALSHGGIANK
VQVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIKAAQFARERDVPYFGICFGMQMAVIEAARNLAGIEQA
NSTEFGPTAEPLVGLMTEWLRGNELERRSNAGDLGGTMRLGAYPAALKRGSRVSKIYGDVLEISERHRHRYEVNTAYKDR
LEQHGLRFSGMSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFSSFIEAALVRSRLV
>Mature_542_residues
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGR
PATRQDNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFIVDSNDGYDFVLVEIGGTVGDIEGLPFFEAIRQIKN
DLPRGDVIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDAD
SIYAVPEAYHAAGLDDEVLAAFAIAAEQPPALERWHQINERIRNPEGAVTIAIVGKYTGMKDAYKSLIEALSHGGIANKV
QVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIKAAQFARERDVPYFGICFGMQMAVIEAARNLAGIEQAN
STEFGPTAEPLVGLMTEWLRGNELERRSNAGDLGGTMRLGAYPAALKRGSRVSKIYGDVLEISERHRHRYEVNTAYKDRL
EQHGLRFSGMSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFSSFIEAALVRSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=559, Percent_Identity=44.0071556350626, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=561, Percent_Identity=43.49376114082, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=561, Percent_Identity=43.49376114082, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=561, Percent_Identity=43.49376114082, Blast_Score=470, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=56.610800744879, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI25148299, Length=610, Percent_Identity=37.2131147540984, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=40.2802101576182, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=40.2116402116402, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=44.1860465116279, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=43.3663366336634, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60275; Mature: 60144

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00442 GATASE_TYPE_I ; PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPATRQDNITTGRIYQDILTKERRGDYLGATIQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
AIKDFIVDSNDGYDFVLVEIGGTVGDIEGLPFFEAIRQIKNDLPRGDVIYIHLTLLPYIP
HHHHHEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCEEEECCCCEEEEECCC
DSIYAVPEAYHAAGLDDEVLAAFAIAAEQPPALERWHQINERIRNPEGAVTIAIVGKYTG
CCEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
MKDAYKSLIEALSHGGIANKVQVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCC
GKIKAAQFARERDVPYFGICFGMQMAVIEAARNLAGIEQANSTEFGPTAEPLVGLMTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGNELERRSNAGDLGGTMRLGAYPAALKRGSRVSKIYGDVLEISERHRHRYEVNTAYKDR
CCCCHHHHCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHH
LEQHGLRFSGMSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFSSFIEAALVRS
HHHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
HCC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPATRQDNITTGRIYQDILTKERRGDYLGATIQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
AIKDFIVDSNDGYDFVLVEIGGTVGDIEGLPFFEAIRQIKNDLPRGDVIYIHLTLLPYIP
HHHHHEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCEEEECCCCEEEEECCC
DSIYAVPEAYHAAGLDDEVLAAFAIAAEQPPALERWHQINERIRNPEGAVTIAIVGKYTG
CCEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
MKDAYKSLIEALSHGGIANKVQVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCC
GKIKAAQFARERDVPYFGICFGMQMAVIEAARNLAGIEQANSTEFGPTAEPLVGLMTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGNELERRSNAGDLGGTMRLGAYPAALKRGSRVSKIYGDVLEISERHRHRYEVNTAYKDR
CCCCHHHHCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHH
LEQHGLRFSGMSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFSSFIEAALVRS
HHHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]