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The map label for this gene is pcm2

Identifier: 86749854

GI number: 86749854

Start: 3122296

End: 3122949

Strand: Reverse

Name: pcm2

Synonym: RPB_2737

Alternate gene names: 86749854

Gene position: 3122949-3122296 (Counterclockwise)

Preceding gene: 86749856

Following gene: 86749853

Centisome position: 58.57

GC content: 68.65

Gene sequence:

>654_bases
ATGATCAGTTCCGTGGCGCCGCCGCCGGAAAAGATGCTGTTTCAATTGAGCCTGCGCCGCCGCGGGATTAGCGATCGCGG
CGTGCTGCAGGCGATGGAATCGGTGCCGCGCGACCGTTTCGTCGACGCGGTGCATCGTGACAGTGCCTGGCGCGACACCG
CGCTGCCGATCGCCTGCGGCCAGACCATCAGCCAGCCCTTCGTCGTCGCCTATATGACCGAACAGCTTCATCTCCAGCCC
GGCCACCGCGTGCTCGAGATCGGCACCGGCTCCGGTTACCACGCCGCGGTGCTGTCGCGGCTGGTGCGCGACGTCGTCAG
CGTCGAGCGCTTCAAGACGCTCGCCGACCGCGCCCGCGCCCGGCTGAAGGAATTGAACTACGCCAATGTCGAGGTGGTGC
TCGGCGACGGCTTCGCGCTGCCGGAGGGGCAGGGTACCTTCGACCGCATCCTGGTGACCGCGGCGATGGCCGAACTGCCG
CAGCCGTTGCTCGATCTGCTCGACCCCGACGGCATCCTGATCGCACCGATCGGGCCGGGAAACGGGCGGCAGACGCTGAT
CCGGGTGCAGCGCAAGGACGACGGCTTCCTGCGCAAGCCGCTGGTCGACGTGCGCTTCGTGCCGGCGCTGCCGGGCATCG
CCCGCGAGCTCTGA

Upstream 100 bases:

>100_bases
TGCCAGAAATCGCTCGGCCATCCAACAATTTGATCGAATCGGGGAATTCGGCGATGGAACCCGGGCCGCTGCGCTCCCGT
GCCGGCCCGAGCGAGGGGCA

Downstream 100 bases:

>100_bases
GCGTCGTCCGTCAAGGCCCGTCGCGGCGCAGGGTTAAACGCTTGTTTACTGCCGACGTGTTTACTCAATTTACCTTGAGT
TGCGTATGAGTGAGTAACCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MISSVAPPPEKMLFQLSLRRRGISDRGVLQAMESVPRDRFVDAVHRDSAWRDTALPIACGQTISQPFVVAYMTEQLHLQP
GHRVLEIGTGSGYHAAVLSRLVRDVVSVERFKTLADRARARLKELNYANVEVVLGDGFALPEGQGTFDRILVTAAMAELP
QPLLDLLDPDGILIAPIGPGNGRQTLIRVQRKDDGFLRKPLVDVRFVPALPGIAREL

Sequences:

>Translated_217_residues
MISSVAPPPEKMLFQLSLRRRGISDRGVLQAMESVPRDRFVDAVHRDSAWRDTALPIACGQTISQPFVVAYMTEQLHLQP
GHRVLEIGTGSGYHAAVLSRLVRDVVSVERFKTLADRARARLKELNYANVEVVLGDGFALPEGQGTFDRILVTAAMAELP
QPLLDLLDPDGILIAPIGPGNGRQTLIRVQRKDDGFLRKPLVDVRFVPALPGIAREL
>Mature_217_residues
MISSVAPPPEKMLFQLSLRRRGISDRGVLQAMESVPRDRFVDAVHRDSAWRDTALPIACGQTISQPFVVAYMTEQLHLQP
GHRVLEIGTGSGYHAAVLSRLVRDVVSVERFKTLADRARARLKELNYANVEVVLGDGFALPEGQGTFDRILVTAAMAELP
QPLLDLLDPDGILIAPIGPGNGRQTLIRVQRKDDGFLRKPLVDVRFVPALPGIAREL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1789100, Length=196, Percent_Identity=49.4897959183673, Blast_Score=174, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17981723, Length=222, Percent_Identity=30.1801801801802, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT2_RHOP2 (Q2IWH1)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486350.1
- ProteinModelPortal:   Q2IWH1
- STRING:   Q2IWH1
- GeneID:   3910530
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2737
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   CLSK819579
- BioCyc:   RPAL316058:RPB_2737-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23904; Mature: 23904

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSVAPPPEKMLFQLSLRRRGISDRGVLQAMESVPRDRFVDAVHRDSAWRDTALPIACG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEECC
QTISQPFVVAYMTEQLHLQPGHRVLEIGTGSGYHAAVLSRLVRDVVSVERFKTLADRARA
CCCCCCEEEEEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKELNYANVEVVLGDGFALPEGQGTFDRILVTAAMAELPQPLLDLLDPDGILIAPIGPG
HHHHCCCCCEEEEEECCEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCC
NGRQTLIRVQRKDDGFLRKPLVDVRFVPALPGIAREL
CCCEEEEEEECCCCCCHHCCCCCEEECCCCCCHHHCC
>Mature Secondary Structure
MISSVAPPPEKMLFQLSLRRRGISDRGVLQAMESVPRDRFVDAVHRDSAWRDTALPIACG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEECC
QTISQPFVVAYMTEQLHLQPGHRVLEIGTGSGYHAAVLSRLVRDVVSVERFKTLADRARA
CCCCCCEEEEEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKELNYANVEVVLGDGFALPEGQGTFDRILVTAAMAELPQPLLDLLDPDGILIAPIGPG
HHHHCCCCCEEEEEECCEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCC
NGRQTLIRVQRKDDGFLRKPLVDVRFVPALPGIAREL
CCCEEEEEEECCCCCCHHCCCCCEEECCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA