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The map label for this gene is gph [C]

Identifier: 86749760

GI number: 86749760

Start: 3023883

End: 3024566

Strand: Direct

Name: gph [C]

Synonym: RPB_2643

Alternate gene names: 86749760

Gene position: 3023883-3024566 (Clockwise)

Preceding gene: 86749759

Following gene: 86749761

Centisome position: 56.72

GC content: 62.87

Gene sequence:

>684_bases
ATGAAAGCATTTTTCTTCGACCTCGATGGCACGCTGACCGACTCGCGGACGGGGCTGCACGATTCGTTTCGCGCGGCGAT
CGAAGCGATCGGCGTGATGCCGCTTGGCGACGCCGAGCTTGACCTGTTCCTAGGCACGCCTCTGCCGGAGATGTTCCGAA
CGTTGAAGGCCGACATCAGCCAGGCCGGAATCGAGGCCGGCATCGACGCTTTCCGGGCTTACTACGAAACCCACGGTATC
CGTCAAAACCGTGCCTATCCCGGAGTTCGGGAGATGCTGGAGGCTTTGAAGACCAGACGGATTCCTGTCTGGGTCGTGAC
CTCCAAGCCCCAGGCCTATGCCGAGCGTGTGATCCGCGATCTAGGCTTTGCCGATTTGGTCACCGACGTCGTCGGCGCGG
GGCTCGCGGAGTTGGACACTAAAAGCGATCTGGTGAAGCGCGCGCTCGGCGAAGCGGGCGTCGCCCCTGGCGATGCCGTG
ATGGTCGGAGACCGGCGCTATGATGTCGAAGGGGCCAAGGCCAATGGTGTCGTCGCTGTCGGCGTGACTTGGGGCTACGG
CACGCGCGAAGAACTCGAGTCCGCGGGCTGCGATCGCCTCGTCGAGTCCGTTTCGGAGTTCTGTCAGATCTTCATCGATC
GCTCGATCGTGCTTCGTACCAGGACCGCCGCCGCGGCCTGCTGA

Upstream 100 bases:

>100_bases
CGTTCTGGCCGGAGCGGACAAAGATCCTGCGCCCGCGGCGCGGGGCTGGTTCACGCCAGAGGTCGTCTCGAAGTTCAACA
AGCACGCCTAGGCTGATGCG

Downstream 100 bases:

>100_bases
ACCGGTCGCCATGTCGGCGGAGCCCGCAGGCATCCTTGCGCACGCTCATGAGTGGCGCGCGCGGCTGCGAACTGAACCAA
TCGGAACCCGGCGCGTTACC

Product: HAD family hydrolase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKAFFFDLDGTLTDSRTGLHDSFRAAIEAIGVMPLGDAELDLFLGTPLPEMFRTLKADISQAGIEAGIDAFRAYYETHGI
RQNRAYPGVREMLEALKTRRIPVWVVTSKPQAYAERVIRDLGFADLVTDVVGAGLAELDTKSDLVKRALGEAGVAPGDAV
MVGDRRYDVEGAKANGVVAVGVTWGYGTREELESAGCDRLVESVSEFCQIFIDRSIVLRTRTAAAAC

Sequences:

>Translated_227_residues
MKAFFFDLDGTLTDSRTGLHDSFRAAIEAIGVMPLGDAELDLFLGTPLPEMFRTLKADISQAGIEAGIDAFRAYYETHGI
RQNRAYPGVREMLEALKTRRIPVWVVTSKPQAYAERVIRDLGFADLVTDVVGAGLAELDTKSDLVKRALGEAGVAPGDAV
MVGDRRYDVEGAKANGVVAVGVTWGYGTREELESAGCDRLVESVSEFCQIFIDRSIVLRTRTAAAAC
>Mature_227_residues
MKAFFFDLDGTLTDSRTGLHDSFRAAIEAIGVMPLGDAELDLFLGTPLPEMFRTLKADISQAGIEAGIDAFRAYYETHGI
RQNRAYPGVREMLEALKTRRIPVWVVTSKPQAYAERVIRDLGFADLVTDVVGAGLAELDTKSDLVKRALGEAGVAPGDAV
MVGDRRYDVEGAKANGVVAVGVTWGYGTREELESAGCDRLVESVSEFCQIFIDRSIVLRTRTAAAAC

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=225, Percent_Identity=24.8888888888889, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 24609; Mature: 24609

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFFFDLDGTLTDSRTGLHDSFRAAIEAIGVMPLGDAELDLFLGTPLPEMFRTLKADIS
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
QAGIEAGIDAFRAYYETHGIRQNRAYPGVREMLEALKTRRIPVWVVTSKPQAYAERVIRD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LGFADLVTDVVGAGLAELDTKSDLVKRALGEAGVAPGDAVMVGDRRYDVEGAKANGVVAV
CCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCCCCCEEEE
GVTWGYGTREELESAGCDRLVESVSEFCQIFIDRSIVLRTRTAAAAC
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEECHHHHCC
>Mature Secondary Structure
MKAFFFDLDGTLTDSRTGLHDSFRAAIEAIGVMPLGDAELDLFLGTPLPEMFRTLKADIS
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
QAGIEAGIDAFRAYYETHGIRQNRAYPGVREMLEALKTRRIPVWVVTSKPQAYAERVIRD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LGFADLVTDVVGAGLAELDTKSDLVKRALGEAGVAPGDAVMVGDRRYDVEGAKANGVVAV
CCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCCCCCEEEE
GVTWGYGTREELESAGCDRLVESVSEFCQIFIDRSIVLRTRTAAAAC
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]