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The map label for this gene is tuf1 [H]

Identifier: 86749413

GI number: 86749413

Start: 2652673

End: 2653863

Strand: Direct

Name: tuf1 [H]

Synonym: RPB_2293

Alternate gene names: 86749413

Gene position: 2652673-2653863 (Clockwise)

Preceding gene: 86749412

Following gene: 86749414

Centisome position: 49.75

GC content: 64.48

Gene sequence:

>1191_bases
ATGGCCAAAGCAAAATTCGAACGTACCAAGCCGCATTGCAATATCGGGACGATCGGTCACGTCGACCATGGCAAGACGTC
GCTGACGGCGGCGATCACCAAGGTTCTGGCGGAGACGGGCGGCGCGACGTTCACGGCCTACGACCAGATCGACAAGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATTTCGACGGCTCACGTCGAATACGAGACGACGAACCGCCACTACGCGCATGTC
GACTGCCCGGGCCACGCCGACTACGTCAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGGCATTCTGGTGGTGTC
GGCGGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTGGCGCGCCAGGTCGGCGTTCCGGCGCTGGTGGTGT
TCCTGAACAAGTGCGACATGGTCGACGATCCGGAACTGCTCGAGCTGGTCGAGATGGAAGTGCGCGAGCTTCTGTCGAAG
TACGACTTCCCGGGCGACGACATTCCGATCATCAAGGGCTCGGCGCTGGCCGCTCTGGAGAACTCGGATCAGAAGCTCGG
CCACGACGCGATCCTGGAGCTGATGAAGGCGGTCGACGCCTACATTCCGCAGCCGGAGCGCCCGATCGACCAGCCGTTCC
TGATGCCGGTCGAAGACGTGTTCTCGATCTCGGGCCGCGGCACGGTGGTGACCGGCCGCGTCGAGCGCGGCATCGTCAAG
GTCGGCGAGGAAATCGAGATCGTCGGCATCCGCGACACCCAGAAGACGACCTGCACGGGCGTCGAAATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCCGGCGACAACATCGGCTGCCTGCTGCGCGGCACCAAGCGCGAAGACGTCGAGCGCGGCCAGG
TGCTGTGCAAGCCGGGTTCGGTGAAGCCGCACACCAAGTTCAAGGCCGAGGCCTACATCCTGACCAAGGAAGAGGGCGGC
CGTCACACCCCGTTCTTCACCAACTATCGTCCGCAGTTCTACTTCCGCACCACCGACGTCACCGGCGTCGTGCATCTGCC
GGAAGGCACCGAGATGGTGATGCCGGGCGACAACATCGCCATGGAAGTGCACCTGATCGTGCCGATCGCGATGGAAGAAA
AGCTGCGCTTCGCCATCCGCGAAGGCGGCCGCACCGTCGGCGCCGGCGTGGTGGCCTCGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CGACCACTACGCGGAAGCCCCGGCCAACGTGTCGGCGGAAGTCCAGAAGAAGTTTGCCTGATTGTCGCCGGCGTAAGCCG
CGACTGAACGGAGAAGAACG

Downstream 100 bases:

>100_bases
GGCGAATAGCGAATAGAGAGTGGCGGACGGATCCCCGCTCGCCACTCACTACTCACCATTGGCTCACTGAAGAAAGATAC
GGCAATGAACGGCCAGAATA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVEMEVRELLSK
YDFPGDDIPIIKGSALAALENSDQKLGHDAILELMKAVDAYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNIGCLLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVEMEVRELLSK
YDFPGDDIPIIKGSALAALENSDQKLGHDAILELMKAVDAYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNIGCLLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVEMEVRELLSKY
DFPGDDIPIIKGSALAALENSDQKLGHDAILELMKAVDAYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGIVKV
GEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNIGCLLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=30.0448430493274, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=30.0448430493274, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=29.3453724604966, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=29.1021671826625, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=29.9382716049383, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=29.1021671826625, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=29.1021671826625, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=27.0440251572327, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI157426893, Length=307, Percent_Identity=27.6872964169381, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI53729339, Length=238, Percent_Identity=31.9327731092437, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=238, Percent_Identity=31.9327731092437, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.4683544303797, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.4683544303797, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=31.1345646437995, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=33.6099585062241, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=27.4626865671642, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.75, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.3571428571429, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.3571428571429, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=426, Percent_Identity=29.1079812206573, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=430, Percent_Identity=27.2093023255814, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.2093023255814, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=28.4090909090909, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.5234899328859, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=37.5838926174497, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=26.0714285714286, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=239, Percent_Identity=24.2677824267782, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.5879396984925, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.9319727891156, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.9319727891156, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=26.2108262108262, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=27.2463768115942, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=31.2849162011173, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=26.8595041322314, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.5241730279898, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.5241730279898, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.4791666666667, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.3482142857143, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.3482142857143, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.3482142857143, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.3482142857143, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.3531353135314, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=29.8816568047337, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=28.486646884273, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=26.7857142857143, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=26.7857142857143, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=35.7575757575758, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43330; Mature: 43199

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETTNRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDPELLELVEMEVRELLSKYDFPGDDIPIIKGSALAALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
NSDQKLGHDAILELMKAVDAYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHC
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNIGCLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETTNRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDPELLELVEMEVRELLSKYDFPGDDIPIIKGSALAALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
NSDQKLGHDAILELMKAVDAYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHC
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNIGCLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA