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The map label for this gene is gyaR [H]

Identifier: 86749399

GI number: 86749399

Start: 2627788

End: 2628735

Strand: Direct

Name: gyaR [H]

Synonym: RPB_2279

Alternate gene names: 86749399

Gene position: 2627788-2628735 (Clockwise)

Preceding gene: 86749398

Following gene: 86749400

Centisome position: 49.29

GC content: 67.3

Gene sequence:

>948_bases
GTGACGGACAAGGTTCTGGTGTTTTCGCGGTTCGCGAAGTCGATGCTGACGCGGTTCGGCGAGCGGTTCGAACTGCTCGA
GACCGGCGGCAAGCCGGCCGACGAAGTCTTCAGCGCGACCGAACTCGCCGGCGTTCGCGCGCTGCTGACCATGGGCGCGC
AGCCGCTCGGCCGCGACACGATGGATCTGTTGCCGTCGCTCGGCGCGATCGTCTGCTACGGCACCGGCTATGACGGAGTC
GACCTCGACGCCGCCGCCGAACGCAACATCCTGATCGGCAACTCGCCGGCGGCGAATGCCTCGGCGGTCGCCGATCTGGC
GATGACGCTGCTGCTCGGGCTGATGCGACGGGTGATTCCGGCGGACGCTTACTTACGCAGCGGCGGCTGGTCGGGAGCGA
GGCCGTCGCCGCTGCTCAAGCCACCGCGCGGCCTGACCGGCGCCAAAGTCGGCGTCTACGGCATGGGCGAGATCGGCCGC
AAGATCGCGGCGCGGGTGGCGTCGTTCGAGACCGAGGTCGCCTATCACAGCCGCAGCCGTCACGACGTGCCGTATCGCTA
TGTCGGGAGCTTAAGCGAACTGGTCGACTGGTGCGACGTGCTGCTGATCGCGGTCCGCGCCGGCCCGGATACGCAGCGCA
TCATCGATGCGGAGATGTTGAAGCGGCTCGGCAAGGACGGCGTGGTGGTCAACATCTCGCGCGGCTCGGTGATCGACCAG
CCGGCGCTGATCGCGGCACTGGCCGACAACACCATCGCCGGCGCCGGGCTCGACGTGTTCGAACAGGAGCCTTACGTGCC
GGATGCGCTCAGCGAGTTCCCGCACGTGGTGCTGACGCCGCATATCGGCGGTCACACCCTCGATGCGCATGTCGCGATGC
AGGATTGCGTGATCGCCAACCTCACAGCTTACTTTGCAGGCAGGCCGCTGCCTTATCCGGTGGCGTAG

Upstream 100 bases:

>100_bases
CCTTGCGGCAAACTCGAGGCAAACCAGGGGCGGATGGGGCAAACCCGTCCGCCCCTTTGTCTTTGCTTCCAAGATCAAGA
AACGAGCGAAGGAGCCCGGT

Downstream 100 bases:

>100_bases
TTCATCGCGCAACTGTCATAAATCAGTGCGTAGCGCGCATCGATGATGCAGCCGCTGACGGCGCGTGGCATCACACGAGC
CTGATTGTGTAAACACATAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTDKVLVFSRFAKSMLTRFGERFELLETGGKPADEVFSATELAGVRALLTMGAQPLGRDTMDLLPSLGAIVCYGTGYDGV
DLDAAAERNILIGNSPAANASAVADLAMTLLLGLMRRVIPADAYLRSGGWSGARPSPLLKPPRGLTGAKVGVYGMGEIGR
KIAARVASFETEVAYHSRSRHDVPYRYVGSLSELVDWCDVLLIAVRAGPDTQRIIDAEMLKRLGKDGVVVNISRGSVIDQ
PALIAALADNTIAGAGLDVFEQEPYVPDALSEFPHVVLTPHIGGHTLDAHVAMQDCVIANLTAYFAGRPLPYPVA

Sequences:

>Translated_315_residues
MTDKVLVFSRFAKSMLTRFGERFELLETGGKPADEVFSATELAGVRALLTMGAQPLGRDTMDLLPSLGAIVCYGTGYDGV
DLDAAAERNILIGNSPAANASAVADLAMTLLLGLMRRVIPADAYLRSGGWSGARPSPLLKPPRGLTGAKVGVYGMGEIGR
KIAARVASFETEVAYHSRSRHDVPYRYVGSLSELVDWCDVLLIAVRAGPDTQRIIDAEMLKRLGKDGVVVNISRGSVIDQ
PALIAALADNTIAGAGLDVFEQEPYVPDALSEFPHVVLTPHIGGHTLDAHVAMQDCVIANLTAYFAGRPLPYPVA
>Mature_314_residues
TDKVLVFSRFAKSMLTRFGERFELLETGGKPADEVFSATELAGVRALLTMGAQPLGRDTMDLLPSLGAIVCYGTGYDGVD
LDAAAERNILIGNSPAANASAVADLAMTLLLGLMRRVIPADAYLRSGGWSGARPSPLLKPPRGLTGAKVGVYGMGEIGRK
IAARVASFETEVAYHSRSRHDVPYRYVGSLSELVDWCDVLLIAVRAGPDTQRIIDAEMLKRLGKDGVVVNISRGSVIDQP
ALIAALADNTIAGAGLDVFEQEPYVPDALSEFPHVVLTPHIGGHTLDAHVAMQDCVIANLTAYFAGRPLPYPVA

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=290, Percent_Identity=31.0344827586207, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI23308577, Length=274, Percent_Identity=28.4671532846715, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=27.4809160305344, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=27.4809160305344, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=26.3358778625954, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=26.3358778625954, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI145580575, Length=266, Percent_Identity=27.0676691729323, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI87082289, Length=319, Percent_Identity=28.8401253918495, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=34.2412451361868, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787645, Length=259, Percent_Identity=27.027027027027, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081824, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=241, Percent_Identity=31.1203319502075, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=241, Percent_Identity=25.3112033195021, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=249, Percent_Identity=36.9477911646586, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320925, Length=324, Percent_Identity=28.3950617283951, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=294, Percent_Identity=29.2517006802721, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=279, Percent_Identity=24.7311827956989, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571528, Length=289, Percent_Identity=31.4878892733564, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI45552429, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585514, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574282, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574284, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45551003, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574286, Length=302, Percent_Identity=29.1390728476821, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19921140, Length=225, Percent_Identity=30.6666666666667, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585516, Length=287, Percent_Identity=26.8292682926829, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646446, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646448, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646452, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646450, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI62472511, Length=237, Percent_Identity=28.6919831223629, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 33516; Mature: 33385

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKVLVFSRFAKSMLTRFGERFELLETGGKPADEVFSATELAGVRALLTMGAQPLGRDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MDLLPSLGAIVCYGTGYDGVDLDAAAERNILIGNSPAANASAVADLAMTLLLGLMRRVIP
HHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
ADAYLRSGGWSGARPSPLLKPPRGLTGAKVGVYGMGEIGRKIAARVASFETEVAYHSRSR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
HDVPYRYVGSLSELVDWCDVLLIAVRAGPDTQRIIDAEMLKRLGKDGVVVNISRGSVIDQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PALIAALADNTIAGAGLDVFEQEPYVPDALSEFPHVVLTPHIGGHTLDAHVAMQDCVIAN
CHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHH
LTAYFAGRPLPYPVA
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDKVLVFSRFAKSMLTRFGERFELLETGGKPADEVFSATELAGVRALLTMGAQPLGRDT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MDLLPSLGAIVCYGTGYDGVDLDAAAERNILIGNSPAANASAVADLAMTLLLGLMRRVIP
HHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
ADAYLRSGGWSGARPSPLLKPPRGLTGAKVGVYGMGEIGRKIAARVASFETEVAYHSRSR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
HDVPYRYVGSLSELVDWCDVLLIAVRAGPDTQRIIDAEMLKRLGKDGVVVNISRGSVIDQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PALIAALADNTIAGAGLDVFEQEPYVPDALSEFPHVVLTPHIGGHTLDAHVAMQDCVIAN
CHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHH
LTAYFAGRPLPYPVA
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA