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The map label for this gene is nodT [H]

Identifier: 86749360

GI number: 86749360

Start: 2566836

End: 2568392

Strand: Direct

Name: nodT [H]

Synonym: RPB_2240

Alternate gene names: 86749360

Gene position: 2566836-2568392 (Clockwise)

Preceding gene: 86749359

Following gene: 86749361

Centisome position: 48.14

GC content: 67.05

Gene sequence:

>1557_bases
ATGAGTTCAGATCGGATCGTTCCGATATGCGTCGGTACGCCGTCGACGCGTCGTGAAACCTCGCGGCGACCTGTTCGGTG
CCGGGCGTCGCATATCGCAGTGCTGTTGCTGTGCGCAACCGCGCTGTCCGGCTGTGCCGTCGGCCCGGACTATGTCGTGC
CCGGAATCGAGCTGCCGACCGCCTGGCGCAACGCCAGACCGCCGAAGACACCGCCCGCTGTCCCGCAATTGAGCGAGTGG
TGGCGGCGTTTGAACGATCAGTTGCTGAACCGGCTGATCGAGGAGGCGGTGGCGAGCAATCTCGACGTCGCCAGCGCGAA
GGCGCGGATCCGGGAAGCGCGTGCGACCCGCCGACAGGCGATCGGCGCGTTGTTTCCGACGCTCGATGGGTCCGGGTCGG
CGACGCGCAATCAGGTCAGTGCGAGCTCCACATCCGGCGGTTCGGCGATCACGACCTACAACCAGTTCCAGGCCGGGTTC
GATTCCAGTTGGGAGCTGGACATTTTCGGCGCCAACCGCCGCAACGTCGAGGCGACGACTTACGGCGTCGACGTCGCCGA
GGACGATCTGCGCGCGACCTTGCTGACGCTGATCGGTGACGTCACGTCCTACTACGCGCAGGCGCGCGGCTATCAGGCCC
GCATCGCTCTGGCGCGCCGGACGGCAGCCTCGCAACGCGAGACGGCAGCGTTGACGCAGCGCAAGTTCGACGCCGGCTCC
TCGTCGGCCGTCGACGTCGCGAAGGCCGCGGCGCAGGCCGCCTCGACCGAAGCCAATATTCCGACCTTCGAGATCTCGTA
TGCGGAATCGGTTCACCGGCTCGGCGTGTTGCTGGGCCGTGAGCCGTCTGCGCTGTCGCCGCTGCTCGCCGGCGTCAAGC
CGATCCCGGCGCCGCGTTTGCCGTTGCCGAAGGGCATCCCCGCCGACGTGCTGGTGATGCGGCCGGACGTGCGCAAGGCC
GAACGGCAACTGGCGCAATATACGGCGTTGATCGGCAAGGCCGAAGCCGCGCTCTATCCGTCGGTGAGTCTGACCGGTTC
GGTTTCGACGACCGCGCTGAAGATCGGCGACCTCGGCAAGAACTCGACGATCGGCTGGTCGATCGGGCCGAGCCTGAGCG
TGCCGATCTTCAACGGCGGACAATTGCGCGCCGCAGTCGAGATCAGCGAAGCCCAGCGCGATCAGTACTATGTCGCCTGG
CGCGCGGCGGTGCTGTCGGCGTTGGAGGATGTCGAGAACGCGATCGTCTCGCTGTCGCAGCAGCGCAAGCGGATCGGCAA
TTTGTCGGAATCGGCGCGGCGCTACGCCGAGGCGGCCCGTCTGTCGCGCTCGCTGTACGAAACCGGGTCGTCGAGCTTCC
TCGACGTTCTCGACGCCGAACGCTCGCTCTACACGGCTGAAGATTCGCTGCTCGACACCAGGGTCTCGGTCGCGACCAGC
TACATCGCTCTCGCCAAGGGGCTCGGCGGCGGCTGGGACGGCATGATCGACAGCTCGGTCCCCGAGGTTGTGGATCTCAA
CACCGGCCCGCATCTGGCCGGGGCGCGGCTCCGCTGA

Upstream 100 bases:

>100_bases
TCTTCGTGCCTTTGCTGTATGTCGGCGTGCGCCGGCTGTTCGATCGCAAGTCGGCGACGGACGAGACTTCCAAAAGCGAT
CCGGCGAAGGGGACGACGGG

Downstream 100 bases:

>100_bases
CGGCGAGGCGATCTGCGGGCTGAGTCGGTGTTGGCCCTTTAAGTACGCGATTCGGGTCAAGGGATCCGCGGAGGAGCGTT
CCGTTCGGATGCGAAGATGG

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSSDRIVPICVGTPSTRRETSRRPVRCRASHIAVLLLCATALSGCAVGPDYVVPGIELPTAWRNARPPKTPPAVPQLSEW
WRRLNDQLLNRLIEEAVASNLDVASAKARIREARATRRQAIGALFPTLDGSGSATRNQVSASSTSGGSAITTYNQFQAGF
DSSWELDIFGANRRNVEATTYGVDVAEDDLRATLLTLIGDVTSYYAQARGYQARIALARRTAASQRETAALTQRKFDAGS
SSAVDVAKAAAQAASTEANIPTFEISYAESVHRLGVLLGREPSALSPLLAGVKPIPAPRLPLPKGIPADVLVMRPDVRKA
ERQLAQYTALIGKAEAALYPSVSLTGSVSTTALKIGDLGKNSTIGWSIGPSLSVPIFNGGQLRAAVEISEAQRDQYYVAW
RAAVLSALEDVENAIVSLSQQRKRIGNLSESARRYAEAARLSRSLYETGSSSFLDVLDAERSLYTAEDSLLDTRVSVATS
YIALAKGLGGGWDGMIDSSVPEVVDLNTGPHLAGARLR

Sequences:

>Translated_518_residues
MSSDRIVPICVGTPSTRRETSRRPVRCRASHIAVLLLCATALSGCAVGPDYVVPGIELPTAWRNARPPKTPPAVPQLSEW
WRRLNDQLLNRLIEEAVASNLDVASAKARIREARATRRQAIGALFPTLDGSGSATRNQVSASSTSGGSAITTYNQFQAGF
DSSWELDIFGANRRNVEATTYGVDVAEDDLRATLLTLIGDVTSYYAQARGYQARIALARRTAASQRETAALTQRKFDAGS
SSAVDVAKAAAQAASTEANIPTFEISYAESVHRLGVLLGREPSALSPLLAGVKPIPAPRLPLPKGIPADVLVMRPDVRKA
ERQLAQYTALIGKAEAALYPSVSLTGSVSTTALKIGDLGKNSTIGWSIGPSLSVPIFNGGQLRAAVEISEAQRDQYYVAW
RAAVLSALEDVENAIVSLSQQRKRIGNLSESARRYAEAARLSRSLYETGSSSFLDVLDAERSLYTAEDSLLDTRVSVATS
YIALAKGLGGGWDGMIDSSVPEVVDLNTGPHLAGARLR
>Mature_517_residues
SSDRIVPICVGTPSTRRETSRRPVRCRASHIAVLLLCATALSGCAVGPDYVVPGIELPTAWRNARPPKTPPAVPQLSEWW
RRLNDQLLNRLIEEAVASNLDVASAKARIREARATRRQAIGALFPTLDGSGSATRNQVSASSTSGGSAITTYNQFQAGFD
SSWELDIFGANRRNVEATTYGVDVAEDDLRATLLTLIGDVTSYYAQARGYQARIALARRTAASQRETAALTQRKFDAGSS
SAVDVAKAAAQAASTEANIPTFEISYAESVHRLGVLLGREPSALSPLLAGVKPIPAPRLPLPKGIPADVLVMRPDVRKAE
RQLAQYTALIGKAEAALYPSVSLTGSVSTTALKIGDLGKNSTIGWSIGPSLSVPIFNGGQLRAAVEISEAQRDQYYVAWR
AAVLSALEDVENAIVSLSQQRKRIGNLSESARRYAEAARLSRSLYETGSSSFLDVLDAERSLYTAEDSLLDTRVSVATSY
IALAKGLGGGWDGMIDSSVPEVVDLNTGPHLAGARLR

Specific function: In Pathogenic Strains It Allows The Bacteria To Invade Brain Microvascular Endothelial Cells (Bmec) Thus Allowing It To Cross The Blood-Brain Barrier And Cause Neonatal Meningitis. [C]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=30.1075268817204, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI2367348, Length=486, Percent_Identity=25.1028806584362, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55306; Mature: 55175

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDRIVPICVGTPSTRRETSRRPVRCRASHIAVLLLCATALSGCAVGPDYVVPGIELPT
CCCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AWRNARPPKTPPAVPQLSEWWRRLNDQLLNRLIEEAVASNLDVASAKARIREARATRRQA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGALFPTLDGSGSATRNQVSASSTSGGSAITTYNQFQAGFDSSWELDIFGANRRNVEATT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEE
YGVDVAEDDLRATLLTLIGDVTSYYAQARGYQARIALARRTAASQRETAALTQRKFDAGS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSAVDVAKAAAQAASTEANIPTFEISYAESVHRLGVLLGREPSALSPLLAGVKPIPAPRL
CHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
PLPKGIPADVLVMRPDVRKAERQLAQYTALIGKAEAALYPSVSLTGSVSTTALKIGDLGK
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCC
NSTIGWSIGPSLSVPIFNGGQLRAAVEISEAQRDQYYVAWRAAVLSALEDVENAIVSLSQ
CCCCCEECCCCCEEEEECCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKRIGNLSESARRYAEAARLSRSLYETGSSSFLDVLDAERSLYTAEDSLLDTRVSVATS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIALAKGLGGGWDGMIDSSVPEVVDLNTGPHLAGARLR
HHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SSDRIVPICVGTPSTRRETSRRPVRCRASHIAVLLLCATALSGCAVGPDYVVPGIELPT
CCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AWRNARPPKTPPAVPQLSEWWRRLNDQLLNRLIEEAVASNLDVASAKARIREARATRRQA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGALFPTLDGSGSATRNQVSASSTSGGSAITTYNQFQAGFDSSWELDIFGANRRNVEATT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEE
YGVDVAEDDLRATLLTLIGDVTSYYAQARGYQARIALARRTAASQRETAALTQRKFDAGS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSAVDVAKAAAQAASTEANIPTFEISYAESVHRLGVLLGREPSALSPLLAGVKPIPAPRL
CHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
PLPKGIPADVLVMRPDVRKAERQLAQYTALIGKAEAALYPSVSLTGSVSTTALKIGDLGK
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCC
NSTIGWSIGPSLSVPIFNGGQLRAAVEISEAQRDQYYVAWRAAVLSALEDVENAIVSLSQ
CCCCCEECCCCCEEEEECCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKRIGNLSESARRYAEAARLSRSLYETGSSSFLDVLDAERSLYTAEDSLLDTRVSVATS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIALAKGLGGGWDGMIDSSVPEVVDLNTGPHLAGARLR
HHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2338917; 2622339 [H]