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The map label for this gene is acrE [H]

Identifier: 86749358

GI number: 86749358

Start: 2562396

End: 2563691

Strand: Direct

Name: acrE [H]

Synonym: RPB_2238

Alternate gene names: 86749358

Gene position: 2562396-2563691 (Clockwise)

Preceding gene: 86749354

Following gene: 86749359

Centisome position: 48.06

GC content: 65.9

Gene sequence:

>1296_bases
TTGACTGCGACATTTGCGGCCCCGAGCCGGCAGGAATGGCCGTTCAAACGCGCACGCTTGGCGATCCTGGGCGTCCCCTT
GGTGGCGGCGCTGCTGCTCGCCGGCTGCGGGCAGGATCAGGCCGCGGCGCCGAAAAACGCGGGTCTTCCCCGGCCGGAGG
TGAGCGTCGTCACGCTGCATCCGCGGTCGGTGGCGATCACCTCGGAATTGTCGGGGCGAACGACGGCGTCGCTGACGGCG
GAGGTGAGGCCCCAGGTCGGCGGCATCATCAAGTCGCGTTTGTATGAAGAGGGCGGCGAAGTCTCGGCCGGTGATGCGTT
GTATCAGATCGACCCGGCGAGCTATCAGGCGACCTACGACAGCGCTGTGGCCGCTCTGGAGAAATCCGAAGCCGCCGTGC
CGAGCGCGGCGGCGAAGGTCGAGCGCTATCAGGGCCTGGTGAAACAGAACGCGGTGTCCAAGCAGGACTACGACGACGCC
GTGGCGACGCTGGCGCAGGCTAAGGCCGACGTCGCCTCCGCCAAGGCGAGCGTCGCGACCGCCCGGATCAATCTCGACTA
CACCAGGATCGTCGCGCCGATCGGCGGCCGGATCGACAAATCGTCTCTCACTCCCGGAGCGCTGGTGACGGCGGGCCAGA
CCACGCTGCTGACGACGATCCGGACGCTGGATCCGATCAATGTCGACGTCACGGAATCCAGCACCAACCTGCTGAACTGG
CGCCAAGCGGTCGATGCCGGTCGCATCAAAGCGACCGGCCCGGATGTCAGCATCAAATTGAAGCTCGACAATGGAACGAC
CTATCCGCTGACCGGCAAGCTCGCCTTCGTCGAATCGAACGTCAGCGAGACCACAGGCACCTTCGCGCTGCGCGCGCAGT
TTCCCAATCCCAAACGTCTGCTGCTGCCCGGCATGTATGTCCGCGCCATCATCGAGCAGGGGATCGCCGAGAACAGCTTC
GTGGTCCCGCAGCGGGCCGTCACGCGCAACACCAAAGGAGAAGCCGTCGCGACGATCGTCACGGCGGAGGGGAAGGTCGA
GCAGCGCGTGCTGACGGTGGGCAACAATGTCGGCAACAACTGGCTGGTCAGCGCTGGCGTCAAGGATGGCGATCGCGTGA
TCGTCGAGGGCATTCAGATGGTCCGTCCGGGGCAGGACGTGACCGCCGTCGAGGTGACGATCGACGAGAACACCGGTCAG
GTCCGCGACCGCAAGCAGGGATCGCTGTCGACCCCGGATCCGGTGCGGTTGGCCGATCGCGACGCCGATCCGAACGCCGG
CGTGGTCGGGAAATAG

Upstream 100 bases:

>100_bases
ATGTCGATCAATTCAGGTCCAACCGCAAATGTTCATTTGACATCCGATCCGATCTGGAAATAACAGTACCGATGCGTACT
GTAAATATGATGAGGATCGC

Downstream 100 bases:

>100_bases
CATGTCGCGTTTTTTCATCGAACGGCCGGTGTTCGCATGGGTGATTGCGATCACCATCATGCTCGGCGGGCTGCTTGCGC
TGCAGACGCTGCCGATCGCG

Product: secretion protein HlyD

Products: NA

Alternate protein names: Protein envC [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTATFAAPSRQEWPFKRARLAILGVPLVAALLLAGCGQDQAAAPKNAGLPRPEVSVVTLHPRSVAITSELSGRTTASLTA
EVRPQVGGIIKSRLYEEGGEVSAGDALYQIDPASYQATYDSAVAALEKSEAAVPSAAAKVERYQGLVKQNAVSKQDYDDA
VATLAQAKADVASAKASVATARINLDYTRIVAPIGGRIDKSSLTPGALVTAGQTTLLTTIRTLDPINVDVTESSTNLLNW
RQAVDAGRIKATGPDVSIKLKLDNGTTYPLTGKLAFVESNVSETTGTFALRAQFPNPKRLLLPGMYVRAIIEQGIAENSF
VVPQRAVTRNTKGEAVATIVTAEGKVEQRVLTVGNNVGNNWLVSAGVKDGDRVIVEGIQMVRPGQDVTAVEVTIDENTGQ
VRDRKQGSLSTPDPVRLADRDADPNAGVVGK

Sequences:

>Translated_431_residues
MTATFAAPSRQEWPFKRARLAILGVPLVAALLLAGCGQDQAAAPKNAGLPRPEVSVVTLHPRSVAITSELSGRTTASLTA
EVRPQVGGIIKSRLYEEGGEVSAGDALYQIDPASYQATYDSAVAALEKSEAAVPSAAAKVERYQGLVKQNAVSKQDYDDA
VATLAQAKADVASAKASVATARINLDYTRIVAPIGGRIDKSSLTPGALVTAGQTTLLTTIRTLDPINVDVTESSTNLLNW
RQAVDAGRIKATGPDVSIKLKLDNGTTYPLTGKLAFVESNVSETTGTFALRAQFPNPKRLLLPGMYVRAIIEQGIAENSF
VVPQRAVTRNTKGEAVATIVTAEGKVEQRVLTVGNNVGNNWLVSAGVKDGDRVIVEGIQMVRPGQDVTAVEVTIDENTGQ
VRDRKQGSLSTPDPVRLADRDADPNAGVVGK
>Mature_430_residues
TATFAAPSRQEWPFKRARLAILGVPLVAALLLAGCGQDQAAAPKNAGLPRPEVSVVTLHPRSVAITSELSGRTTASLTAE
VRPQVGGIIKSRLYEEGGEVSAGDALYQIDPASYQATYDSAVAALEKSEAAVPSAAAKVERYQGLVKQNAVSKQDYDDAV
ATLAQAKADVASAKASVATARINLDYTRIVAPIGGRIDKSSLTPGALVTAGQTTLLTTIRTLDPINVDVTESSTNLLNWR
QAVDAGRIKATGPDVSIKLKLDNGTTYPLTGKLAFVESNVSETTGTFALRAQFPNPKRLLLPGMYVRAIIEQGIAENSFV
VPQRAVTRNTKGEAVATIVTAEGKVEQRVLTVGNNVGNNWLVSAGVKDGDRVIVEGIQMVRPGQDVTAVEVTIDENTGQV
RDRKQGSLSTPDPVRLADRDADPNAGVVGK

Specific function: May affect specific membrane functions, such as septum formation during cell division, and cell membrane permeability [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=47.2, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1786668, Length=371, Percent_Identity=48.5175202156334, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=45.7300275482094, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=30.8411214953271, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87081796, Length=341, Percent_Identity=23.1671554252199, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45402; Mature: 45271

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATFAAPSRQEWPFKRARLAILGVPLVAALLLAGCGQDQAAAPKNAGLPRPEVSVVTLH
CCCCCCCCCCCCCCHHHHHEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
PRSVAITSELSGRTTASLTAEVRPQVGGIIKSRLYEEGGEVSAGDALYQIDPASYQATYD
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHH
SAVAALEKSEAAVPSAAAKVERYQGLVKQNAVSKQDYDDAVATLAQAKADVASAKASVAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
ARINLDYTRIVAPIGGRIDKSSLTPGALVTAGQTTLLTTIRTLDPINVDVTESSTNLLNW
EEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEHHHHCCCCEEEEECCCCHHHHH
RQAVDAGRIKATGPDVSIKLKLDNGTTYPLTGKLAFVESNVSETTGTFALRAQFPNPKRL
HHHHCCCCEEECCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCEE
LLPGMYVRAIIEQGIAENSFVVPQRAVTRNTKGEAVATIVTAEGKVEQRVLTVGNNVGNN
ECCHHHHHHHHHHCCCCCCEECCCHHHHCCCCCCEEEEEEECCCCCHHEEEEECCCCCCC
WLVSAGVKDGDRVIVEGIQMVRPGQDVTAVEVTIDENTGQVRDRKQGSLSTPDPVRLADR
EEEECCCCCCCEEEEECHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECC
DADPNAGVVGK
CCCCCCCCCCC
>Mature Secondary Structure 
TATFAAPSRQEWPFKRARLAILGVPLVAALLLAGCGQDQAAAPKNAGLPRPEVSVVTLH
CCCCCCCCCCCCCHHHHHEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
PRSVAITSELSGRTTASLTAEVRPQVGGIIKSRLYEEGGEVSAGDALYQIDPASYQATYD
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHH
SAVAALEKSEAAVPSAAAKVERYQGLVKQNAVSKQDYDDAVATLAQAKADVASAKASVAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
ARINLDYTRIVAPIGGRIDKSSLTPGALVTAGQTTLLTTIRTLDPINVDVTESSTNLLNW
EEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEHHHHCCCCEEEEECCCCHHHHH
RQAVDAGRIKATGPDVSIKLKLDNGTTYPLTGKLAFVESNVSETTGTFALRAQFPNPKRL
HHHHCCCCEEECCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCEE
LLPGMYVRAIIEQGIAENSFVVPQRAVTRNTKGEAVATIVTAEGKVEQRVLTVGNNVGNN
ECCHHHHHHHHHHCCCCCCEECCCHHHHCCCCCCEEEEEEECCCCCHHEEEEECCCCCCC
WLVSAGVKDGDRVIVEGIQMVRPGQDVTAVEVTIDENTGQVRDRKQGSLSTPDPVRLADR
EEEECCCCCCCEEEEECHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECC
DADPNAGVVGK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1720861; 9278503 [H]