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The map label for this gene is fixK [H]

Identifier: 86749279

GI number: 86749279

Start: 2448605

End: 2449231

Strand: Direct

Name: fixK [H]

Synonym: RPB_2158

Alternate gene names: 86749279

Gene position: 2448605-2449231 (Clockwise)

Preceding gene: 86749278

Following gene: 86749280

Centisome position: 45.93

GC content: 63.48

Gene sequence:

>627_bases
TTGCTGGGGAACGCCGCTGCGATTCGATCGAACACGCAGAGTTTCGCGAAAGGCGAACGAATCTACACCAGGAACGAGAC
GGCGCATCACGCTCACGCTGTCCTTGAAGGTGCGGTTCGGGCCTCGCATTGTCTGCCCGATGGACGCCGTCATCTGAGTG
CGTTTCATCTTCCGGGGGAAATCTTCGGGCTCGACTCCGGCGCATTGCGCAGTCACGAGACCGAAGCCCTGGTGAAGACG
ACCACGCTGGCGGTGCGGCTCGATGATCTCGACCGGGAGGCCGGTATCGATCCCGGCTTGGCCTGGGGGCTCTGCAACCT
CGTCGCCGCCGAGTTGCGGAAGGCCCGTGAGCACATGGTGTTGCTGGGACGCAAGTCGGCGCTGGAACGGGTCGCCGCGT
TCCTGCTCGAGATGGATCGCCGCCTCGCAACCACGGGCGCCATGAGCAAGGGGGGCATGAGTCTGCCGATGTGCGAGCAC
GACATGGCCGATTATCTCGGGCTGAGCAGCGAAACAGTCTCGCGCTGCCTGACCAAGCTTCAAGCGTCGCGCATCATCGT
CATGAGCAAACGACGGACTGTGCGGTTCCTCGATCGTGCCGGCCTGCACGAGCTCGATGCGTCGTGA

Upstream 100 bases:

>100_bases
TCCGGTCTCCTGCATTGGATTGGCTGCCGACATGGCGCTAGCGGACGCGATCATTGTGCCGACGGACGACTGCAGTTCGT
GGCTGGAGACGCATTTCGAT

Downstream 100 bases:

>100_bases
AGACGCCGGCCGCCACCTGGTAATTCATGCGTCCGATCCCTATCTGACACCTATCGTCGCACACGGTTGTCCCGGGCATC
GGCGGCTGAGAGACGCGATC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MLGNAAAIRSNTQSFAKGERIYTRNETAHHAHAVLEGAVRASHCLPDGRRHLSAFHLPGEIFGLDSGALRSHETEALVKT
TTLAVRLDDLDREAGIDPGLAWGLCNLVAAELRKAREHMVLLGRKSALERVAAFLLEMDRRLATTGAMSKGGMSLPMCEH
DMADYLGLSSETVSRCLTKLQASRIIVMSKRRTVRFLDRAGLHELDAS

Sequences:

>Translated_208_residues
MLGNAAAIRSNTQSFAKGERIYTRNETAHHAHAVLEGAVRASHCLPDGRRHLSAFHLPGEIFGLDSGALRSHETEALVKT
TTLAVRLDDLDREAGIDPGLAWGLCNLVAAELRKAREHMVLLGRKSALERVAAFLLEMDRRLATTGAMSKGGMSLPMCEH
DMADYLGLSSETVSRCLTKLQASRIIVMSKRRTVRFLDRAGLHELDAS
>Mature_208_residues
MLGNAAAIRSNTQSFAKGERIYTRNETAHHAHAVLEGAVRASHCLPDGRRHLSAFHLPGEIFGLDSGALRSHETEALVKT
TTLAVRLDDLDREAGIDPGLAWGLCNLVAAELRKAREHMVLLGRKSALERVAAFLLEMDRRLATTGAMSKGGMSLPMCEH
DMADYLGLSSETVSRCLTKLQASRIIVMSKRRTVRFLDRAGLHELDAS

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of nifA expression. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=181, Percent_Identity=27.0718232044199, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 22756; Mature: 22756

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGNAAAIRSNTQSFAKGERIYTRNETAHHAHAVLEGAVRASHCLPDGRRHLSAFHLPGE
CCCCCHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IFGLDSGALRSHETEALVKTTTLAVRLDDLDREAGIDPGLAWGLCNLVAAELRKAREHMV
EEECCCCCCCCCHHHHHHHHHHEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLGRKSALERVAAFLLEMDRRLATTGAMSKGGMSLPMCEHDMADYLGLSSETVSRCLTKL
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
QASRIIVMSKRRTVRFLDRAGLHELDAS
HHHHEEEEECCHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MLGNAAAIRSNTQSFAKGERIYTRNETAHHAHAVLEGAVRASHCLPDGRRHLSAFHLPGE
CCCCCHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IFGLDSGALRSHETEALVKTTTLAVRLDDLDREAGIDPGLAWGLCNLVAAELRKAREHMV
EEECCCCCCCCCHHHHHHHHHHEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLGRKSALERVAAFLLEMDRRLATTGAMSKGGMSLPMCEHDMADYLGLSSETVSRCLTKL
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
QASRIIVMSKRRTVRFLDRAGLHELDAS
HHHHEEEEECCHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766374 [H]