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The map label for this gene is gatA [H]

Identifier: 86749206

GI number: 86749206

Start: 2370632

End: 2371831

Strand: Direct

Name: gatA [H]

Synonym: RPB_2085

Alternate gene names: 86749206

Gene position: 2370632-2371831 (Clockwise)

Preceding gene: 86749205

Following gene: 86749207

Centisome position: 44.46

GC content: 73.08

Gene sequence:

>1200_bases
ATGACGATGCCGGTCGACGACAATCTGAACGCGTTCTGCACCCATGGCCGGGGCGATCGGCGGACGCAGGACAGCGGCGC
GCTGGCCGGGCTGACCTTCGCGGTGAAGGATTTCTTCGACGTCGCGGGTCTGCCGACCGGCGCCGGCAGCCCGGAATGGC
TGGCGACCCATCCGGTGCCGACGCAATCCTCGCCCGTGGTCGACCGGCTGTTCGCCGCCGGCGGCACCATGGTCGGCAAG
ACCCACACCGACGAGATGGCGTGGAGCCTCAATGGCGAGAACGCGCATTACGGCACGCCGATCAATCCGGCCGCGCCGGG
GCGGATTCCGGGCGGCTCGTCGAGCGGCTCGGCCGCGGCCACCGCCGGCGGGCTGGTGGATTTCGCGATCGGCTCGGACA
CCGGCGGCTCGGTGCGGCTGCCGGCGAGCTATTGCGGCGTCTACGGCATCCGCACCACGCATGGGCGGATTCCGCTCGAC
GGCGCGGTGCCGCTGGCGCCGAGTTACGACACCGTCGGCTGGTTTTCGCGCAGCGCTGCGCTGATGGCGCGGGTGGGTGA
GGTGCTGCTCGACGGCGTCCGCGCGCCGCGGCGGCCCAAGCGCGTGCTGATCGCGCGCGATCTGTTCGCCGCGCTCGAGC
CGCGCGTCGTCGAGGTGTTGCAGCCCGGGCTCGCGCAGCTCGCCGCGATGCTCGGCGAGCCCGAGCCGGTCGAGGTCGCG
GGCGATCAGCGCCCGGCATGGCGCAATGCGTTTCGCGTGCTGCAATCCGCCGAGGCCTGGGCGGCGCATGGCGCGTGGGT
GAACGCGGTGAAGCCGGCGTTCGGCCCCGGCGTCAAGGAACGCTTCGCCGCCGCCGCGGTGCTCGATCCGGCCGAAGTCG
TCGCAGCGAAAGCGCTGCGCGACACCATCACGGCGACGATGCGGACGCTGCTGCAGGACGACGCCGTGCTGATCGCGCCG
ACCGCCCCCGGCATCGCGCCGCTGCGCAACAGCACGGGCGAGGCGCTGGAAACCTTCCGCGCCCGCTCGCTCGAACTGCT
CTGCCCCGCCGGCCACGCCGGCCTGCCGCAACTGTCGCTGCCGCTCGCCACGCTCGACGACTGCCCGATCGGCCTCTCGC
TGATCGGCGGTCGCGCCTGCGACGAGGATCTGCTGGCGCTCGCGGCGGAGCTGGAGGCGGGGAGCACGAAGCCGCGCTGA

Upstream 100 bases:

>100_bases
TCGGGCTGAAGCGCGGCCTGCGCACATTCGACAAGCCCGCCGCAATCCTGCACAAGCCGAACTGATCTCGCCGCGCGGCG
ACAACAAGAAACTCACGGAG

Downstream 100 bases:

>100_bases
GGCCGGCGTCGCGCGTCTCACCGCTTCGCCGCGCACCGCGTCGGCCTCGCGCGCCGCTTGACTCGATAAGAACATAGTGT
GAACAATTTCGCCGCGCAGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTMPVDDNLNAFCTHGRGDRRTQDSGALAGLTFAVKDFFDVAGLPTGAGSPEWLATHPVPTQSSPVVDRLFAAGGTMVGK
THTDEMAWSLNGENAHYGTPINPAAPGRIPGGSSSGSAAATAGGLVDFAIGSDTGGSVRLPASYCGVYGIRTTHGRIPLD
GAVPLAPSYDTVGWFSRSAALMARVGEVLLDGVRAPRRPKRVLIARDLFAALEPRVVEVLQPGLAQLAAMLGEPEPVEVA
GDQRPAWRNAFRVLQSAEAWAAHGAWVNAVKPAFGPGVKERFAAAAVLDPAEVVAAKALRDTITATMRTLLQDDAVLIAP
TAPGIAPLRNSTGEALETFRARSLELLCPAGHAGLPQLSLPLATLDDCPIGLSLIGGRACDEDLLALAAELEAGSTKPR

Sequences:

>Translated_399_residues
MTMPVDDNLNAFCTHGRGDRRTQDSGALAGLTFAVKDFFDVAGLPTGAGSPEWLATHPVPTQSSPVVDRLFAAGGTMVGK
THTDEMAWSLNGENAHYGTPINPAAPGRIPGGSSSGSAAATAGGLVDFAIGSDTGGSVRLPASYCGVYGIRTTHGRIPLD
GAVPLAPSYDTVGWFSRSAALMARVGEVLLDGVRAPRRPKRVLIARDLFAALEPRVVEVLQPGLAQLAAMLGEPEPVEVA
GDQRPAWRNAFRVLQSAEAWAAHGAWVNAVKPAFGPGVKERFAAAAVLDPAEVVAAKALRDTITATMRTLLQDDAVLIAP
TAPGIAPLRNSTGEALETFRARSLELLCPAGHAGLPQLSLPLATLDDCPIGLSLIGGRACDEDLLALAAELEAGSTKPR
>Mature_398_residues
TMPVDDNLNAFCTHGRGDRRTQDSGALAGLTFAVKDFFDVAGLPTGAGSPEWLATHPVPTQSSPVVDRLFAAGGTMVGKT
HTDEMAWSLNGENAHYGTPINPAAPGRIPGGSSSGSAAATAGGLVDFAIGSDTGGSVRLPASYCGVYGIRTTHGRIPLDG
AVPLAPSYDTVGWFSRSAALMARVGEVLLDGVRAPRRPKRVLIARDLFAALEPRVVEVLQPGLAQLAAMLGEPEPVEVAG
DQRPAWRNAFRVLQSAEAWAAHGAWVNAVKPAFGPGVKERFAAAAVLDPAEVVAAKALRDTITATMRTLLQDDAVLIAPT
APGIAPLRNSTGEALETFRARSLELLCPAGHAGLPQLSLPLATLDDCPIGLSLIGGRACDEDLLALAAELEAGSTKPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=449, Percent_Identity=23.8307349665924, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI195972892, Length=137, Percent_Identity=32.1167883211679, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=170, Percent_Identity=38.8235294117647, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=173, Percent_Identity=31.7919075144509, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=141, Percent_Identity=34.0425531914894, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=139, Percent_Identity=35.2517985611511, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=447, Percent_Identity=27.5167785234899, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=27.9187817258883, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648113, Length=159, Percent_Identity=41.5094339622642, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644968, Length=420, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648441, Length=411, Percent_Identity=23.3576642335766, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=411, Percent_Identity=23.3576642335766, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648435, Length=411, Percent_Identity=23.3576642335766, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648437, Length=411, Percent_Identity=23.3576642335766, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550774, Length=137, Percent_Identity=32.8467153284672, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 41366; Mature: 41235

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPVDDNLNAFCTHGRGDRRTQDSGALAGLTFAVKDFFDVAGLPTGAGSPEWLATHPVP
CCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
TQSSPVVDRLFAAGGTMVGKTHTDEMAWSLNGENAHYGTPINPAAPGRIPGGSSSGSAAA
CCCCHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
TAGGLVDFAIGSDTGGSVRLPASYCGVYGIRTTHGRIPLDGAVPLAPSYDTVGWFSRSAA
HCCCEEEEEECCCCCCCEECCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMARVGEVLLDGVRAPRRPKRVLIARDLFAALEPRVVEVLQPGLAQLAAMLGEPEPVEVA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEC
GDQRPAWRNAFRVLQSAEAWAAHGAWVNAVKPAFGPGVKERFAAAAVLDPAEVVAAKALR
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
DTITATMRTLLQDDAVLIAPTAPGIAPLRNSTGEALETFRARSLELLCPAGHAGLPQLSL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCC
PLATLDDCPIGLSLIGGRACDEDLLALAAELEAGSTKPR
CCHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMPVDDNLNAFCTHGRGDRRTQDSGALAGLTFAVKDFFDVAGLPTGAGSPEWLATHPVP
CCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
TQSSPVVDRLFAAGGTMVGKTHTDEMAWSLNGENAHYGTPINPAAPGRIPGGSSSGSAAA
CCCCHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
TAGGLVDFAIGSDTGGSVRLPASYCGVYGIRTTHGRIPLDGAVPLAPSYDTVGWFSRSAA
HCCCEEEEEECCCCCCCEECCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMARVGEVLLDGVRAPRRPKRVLIARDLFAALEPRVVEVLQPGLAQLAAMLGEPEPVEVA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEC
GDQRPAWRNAFRVLQSAEAWAAHGAWVNAVKPAFGPGVKERFAAAAVLDPAEVVAAKALR
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
DTITATMRTLLQDDAVLIAPTAPGIAPLRNSTGEALETFRARSLELLCPAGHAGLPQLSL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCC
PLATLDDCPIGLSLIGGRACDEDLLALAAELEAGSTKPR
CCHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA