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The map label for this gene is dmfA2 [H]

Identifier: 86749174

GI number: 86749174

Start: 2331334

End: 2333652

Strand: Direct

Name: dmfA2 [H]

Synonym: RPB_2052

Alternate gene names: 86749174

Gene position: 2331334-2333652 (Clockwise)

Preceding gene: 86749173

Following gene: 86749176

Centisome position: 43.73

GC content: 63.26

Gene sequence:

>2319_bases
ATGGCCGAAAAGAAAGTATTTGGATACGCGGACAAGATTTCGGTCAAGCCCGGCGACGACATCTCGTTCTTCGTGCACGC
CGATGGCACCGACGTCGTCGACGCGCAGCTGGTGCGACTGATCCATGGCGACGCGCATCCGGCCGGACCGGGCTATCGGG
AGGAGGAGATCGCCTGCGAAGCCAACGGGGTGTGGCGGGTTCGCAAGCAGTTCACCCAGGTCGGCTCGTTTCTCACCGTG
GCCGATCCGGAACAGCGCCTCGCGCCGAACGGCAGCTTCAGTCTCTGTATCTTCGTGCATCCGAACAGCCCGGGCGGCGG
CCGACGGCAATGCCTGCTCGGCAAGTGGGACGCGTTCGGCAACCGTGGCTACGGTTTGTGGCTCAACCCGGACGGCTTTC
TCGAATTCGGCTTCGGCGACGGCCACGAGGTCGACTATCTCGACGCCGAAGTGCCGGTTCTGAAGAACAACTGGTACTTC
GTCGCGGCGACGTTCGACGCAACGACCGGCGTGGCGACGCTGTATCAGGAGGGGGTAGCGACCCGCTATAACTCGCTGCT
GAGCAAGGTCGCCAATGTCGACTTCCGCTCGCATGTGCGCGAGACGTTGCGATTCCGCCCGGTCAATCCGCCGGACGTGC
CGTTCCTGCTCGCGGGCGCGCGGGATCATCACACGCTGCGCGGCGATTTCGTCACCCAATGCCTGAACGGCAAACTGGAC
CGGCCCGCGGTGTTCGATCGGGTGCTGACGCATGACGAACTCGACCGCTATCGCGACACCGGGCTGGCGCCGCAGAATGG
CCTGCTGGCCTATTGGGACACGGCGCAGGGCTACACCGCGCAGGGCATCGGCGATCGGGTCATCGATGTCGGGCCCTATG
GATTGCACGCCGAGGGCTACAATCGCCCGGTGCGCGCGCAGACCGGGTTCAACTGGCAGGGCCGCGACGACTGCTTCCGG
CTCGCGCCCGAGCAATATGGTGGCATCGAGTTTCACGACGATGCCATCATCGATTGCAAGTGGGAGCTGACGCGGTCGAT
CAAGCTGCCGGATCTGCGCAGCGGCGCCTATGCGTTCCGGCTGCGCACCGGCGACGCCAAGGGCATGCGCGAGGAATACA
TCGTGTTCTTCGTCCGCTCCGTCGCGCCGAAAGCGCCGATTCTGTTTCTGGTGCCGACGGGCAGCTACCTGGCCTATGCC
AACGAGCATCTCAGTTTCGACGCTGAGATCATGCAGCCGCTCGCCGGACAATCACCGATCCTCTCCGAGGTCGACATCGA
ATTGTACCAGACCGCCGAGTTCGGTCTGTCGCTGTACGACCATCACGCCGATGGCGCAGGCGTCTGCTACAGCACCTATC
GCCGGCCGATCCTGAATATGCGTCCGAAGGCGCGGATGTCGTCGATGGGCGTGACCTGGCAGTTCCCGGCGGATCTGTCG
ATCATCGCCTGGCTCGAGCACATGGGCTACGACTACGATTTGACGACCGACGAGGATCTGCATCGGGAGGGGGCCGATGC
GCTGAAGCCGTACAATGTGGTGCTGAGCGGGACGCATCCGGAATACACCTCGGAAGCGATGCTCGATGCCACCGAAGACT
ACATCGCCGCCGGCGGCCGGTTCATCTATCTCGGCGGCAACGGCTTCTATTGGAACGTCGGCTACCACAGCGATGATCCG
TGGTGCATGGAAGTGCGGAAGCTGAATTCCGGCATGCGGGCATGGCAGGCGCGCCCCGGCGAGTACTATCTGGCGACGAC
CGGTCAGAAGAGCGGCCTGTGGAAGGATCTCGGGCGGCCGCCGCAGAAGATCTTCGGCGTCGGCTTCATCTCGGAAGGTT
TCGATTCGGCGCGGCCGTTCCGGCGGATGCCGGATAGCTGGCATCGCCGCGTGTCATGGATTATGGACGGCATCGAGGGC
GAGATCATCGGCGATTTCGGTCTGGCGCAGGGCGCCGCCGGCGGCATCGAGATTGATCGCTACGATCTGACGCTCGGCAC
CCCGCCGCATTCGCTGATCGTCGCCTCTTCCGGCGGACACAGCGACAACTATCAGACAGTGGTGGAGGAGGTGCTCTATC
CCTATCCAGGGCTGTCCGGATCGCACGACTATCGTGTGCGGGCCGACATGGTCTATTTCACGGCTCCGAATGACGGCGCG
GTGTTTTCGACCGGATCGATCGCCTTCAGTCAATCTTTGCCGTACCAGAATTTCGACAACAACGTATCGCGCCTGCTGGC
GAACGTCGTCACGGCATTCAGCAAGCCGGGGAAACTGCCGGGTTGGGCGTGGTCGGCCGAGGAAAAGCAATGGCGATGA

Upstream 100 bases:

>100_bases
GAGACGCCGGACGAAGCCTATCACGCGCTGTTCCTGCGTCGCGTCCAGGATCTGCTCGAAACCTAATCCGCTCTGTCAGT
CGCTGGAGAAGCCTGAGGCC

Downstream 100 bases:

>100_bases
GTCCGCCGGGCCGGTGAATTTGGGGAGTTGCGGTGGCGTACTCCGCCGCAAACAGCGGCGATCTGTCGCGCGAGGCAAGG
GTCGGTCGTGTTATCTCAGT

Product: large subunit of N,N-dimethylformamidase

Products: NA

Alternate protein names: N,N-dimethylformamidase heavy chain [H]

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MAEKKVFGYADKISVKPGDDISFFVHADGTDVVDAQLVRLIHGDAHPAGPGYREEEIACEANGVWRVRKQFTQVGSFLTV
ADPEQRLAPNGSFSLCIFVHPNSPGGGRRQCLLGKWDAFGNRGYGLWLNPDGFLEFGFGDGHEVDYLDAEVPVLKNNWYF
VAATFDATTGVATLYQEGVATRYNSLLSKVANVDFRSHVRETLRFRPVNPPDVPFLLAGARDHHTLRGDFVTQCLNGKLD
RPAVFDRVLTHDELDRYRDTGLAPQNGLLAYWDTAQGYTAQGIGDRVIDVGPYGLHAEGYNRPVRAQTGFNWQGRDDCFR
LAPEQYGGIEFHDDAIIDCKWELTRSIKLPDLRSGAYAFRLRTGDAKGMREEYIVFFVRSVAPKAPILFLVPTGSYLAYA
NEHLSFDAEIMQPLAGQSPILSEVDIELYQTAEFGLSLYDHHADGAGVCYSTYRRPILNMRPKARMSSMGVTWQFPADLS
IIAWLEHMGYDYDLTTDEDLHREGADALKPYNVVLSGTHPEYTSEAMLDATEDYIAAGGRFIYLGGNGFYWNVGYHSDDP
WCMEVRKLNSGMRAWQARPGEYYLATTGQKSGLWKDLGRPPQKIFGVGFISEGFDSARPFRRMPDSWHRRVSWIMDGIEG
EIIGDFGLAQGAAGGIEIDRYDLTLGTPPHSLIVASSGGHSDNYQTVVEEVLYPYPGLSGSHDYRVRADMVYFTAPNDGA
VFSTGSIAFSQSLPYQNFDNNVSRLLANVVTAFSKPGKLPGWAWSAEEKQWR

Sequences:

>Translated_772_residues
MAEKKVFGYADKISVKPGDDISFFVHADGTDVVDAQLVRLIHGDAHPAGPGYREEEIACEANGVWRVRKQFTQVGSFLTV
ADPEQRLAPNGSFSLCIFVHPNSPGGGRRQCLLGKWDAFGNRGYGLWLNPDGFLEFGFGDGHEVDYLDAEVPVLKNNWYF
VAATFDATTGVATLYQEGVATRYNSLLSKVANVDFRSHVRETLRFRPVNPPDVPFLLAGARDHHTLRGDFVTQCLNGKLD
RPAVFDRVLTHDELDRYRDTGLAPQNGLLAYWDTAQGYTAQGIGDRVIDVGPYGLHAEGYNRPVRAQTGFNWQGRDDCFR
LAPEQYGGIEFHDDAIIDCKWELTRSIKLPDLRSGAYAFRLRTGDAKGMREEYIVFFVRSVAPKAPILFLVPTGSYLAYA
NEHLSFDAEIMQPLAGQSPILSEVDIELYQTAEFGLSLYDHHADGAGVCYSTYRRPILNMRPKARMSSMGVTWQFPADLS
IIAWLEHMGYDYDLTTDEDLHREGADALKPYNVVLSGTHPEYTSEAMLDATEDYIAAGGRFIYLGGNGFYWNVGYHSDDP
WCMEVRKLNSGMRAWQARPGEYYLATTGQKSGLWKDLGRPPQKIFGVGFISEGFDSARPFRRMPDSWHRRVSWIMDGIEG
EIIGDFGLAQGAAGGIEIDRYDLTLGTPPHSLIVASSGGHSDNYQTVVEEVLYPYPGLSGSHDYRVRADMVYFTAPNDGA
VFSTGSIAFSQSLPYQNFDNNVSRLLANVVTAFSKPGKLPGWAWSAEEKQWR
>Mature_771_residues
AEKKVFGYADKISVKPGDDISFFVHADGTDVVDAQLVRLIHGDAHPAGPGYREEEIACEANGVWRVRKQFTQVGSFLTVA
DPEQRLAPNGSFSLCIFVHPNSPGGGRRQCLLGKWDAFGNRGYGLWLNPDGFLEFGFGDGHEVDYLDAEVPVLKNNWYFV
AATFDATTGVATLYQEGVATRYNSLLSKVANVDFRSHVRETLRFRPVNPPDVPFLLAGARDHHTLRGDFVTQCLNGKLDR
PAVFDRVLTHDELDRYRDTGLAPQNGLLAYWDTAQGYTAQGIGDRVIDVGPYGLHAEGYNRPVRAQTGFNWQGRDDCFRL
APEQYGGIEFHDDAIIDCKWELTRSIKLPDLRSGAYAFRLRTGDAKGMREEYIVFFVRSVAPKAPILFLVPTGSYLAYAN
EHLSFDAEIMQPLAGQSPILSEVDIELYQTAEFGLSLYDHHADGAGVCYSTYRRPILNMRPKARMSSMGVTWQFPADLSI
IAWLEHMGYDYDLTTDEDLHREGADALKPYNVVLSGTHPEYTSEAMLDATEDYIAAGGRFIYLGGNGFYWNVGYHSDDPW
CMEVRKLNSGMRAWQARPGEYYLATTGQKSGLWKDLGRPPQKIFGVGFISEGFDSARPFRRMPDSWHRRVSWIMDGIEGE
IIGDFGLAQGAAGGIEIDRYDLTLGTPPHSLIVASSGGHSDNYQTVVEEVLYPYPGLSGSHDYRVRADMVYFTAPNDGAV
FSTGSIAFSQSLPYQNFDNNVSRLLANVVTAFSKPGKLPGWAWSAEEKQWR

Specific function: Hydrolyzes N,N-dimethylformamide, and to a lesser extent N,N-dimethylacetamide and N,N-diethylacetamide. Has no activity against the substituted amides N-methylformamide, N- ethylformamide, N-ethylformamide and N-methylacetamide or the unsubstituted amide

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985 [H]

Pfam domain/function: NA

EC number: =3.5.1.56 [H]

Molecular weight: Translated: 86094; Mature: 85963

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKVFGYADKISVKPGDDISFFVHADGTDVVDAQLVRLIHGDAHPAGPGYREEEIACE
CCCCCEECCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEC
ANGVWRVRKQFTQVGSFLTVADPEQRLAPNGSFSLCIFVHPNSPGGGRRQCLLGKWDAFG
CCCHHHHHHHHHHHCCEEEECCCHHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCC
NRGYGLWLNPDGFLEFGFGDGHEVDYLDAEVPVLKNNWYFVAATFDATTGVATLYQEGVA
CCCEEEEECCCCCEEECCCCCCCEEEECCCCCEEECCEEEEEEECCCCCHHHHHHHHHHH
TRYNSLLSKVANVDFRSHVRETLRFRPVNPPDVPFLLAGARDHHTLRGDFVTQCLNGKLD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCC
RPAVFDRVLTHDELDRYRDTGLAPQNGLLAYWDTAQGYTAQGIGDRVIDVGPYGLHAEGY
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCEEEECCCCEECCCC
NRPVRAQTGFNWQGRDDCFRLAPEQYGGIEFHDDAIIDCKWELTRSIKLPDLRSGAYAFR
CCCEECCCCCCCCCCCHHHHCCHHHCCCEEECCCCEEEEEEEEECCCCCCCCCCCCEEEE
LRTGDAKGMREEYIVFFVRSVAPKAPILFLVPTGSYLAYANEHLSFDAEIMQPLAGQSPI
EECCCCCCCHHHHHEEEEHHCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCC
LSEVDIELYQTAEFGLSLYDHHADGAGVCYSTYRRPILNMRPKARMSSMGVTWQFPADLS
CHHCCEEEEEEHHHCCEEEECCCCCCCEEHHHHCCCCCCCCCCHHHHHCCEEEECCCCCH
IIAWLEHMGYDYDLTTDEDLHREGADALKPYNVVLSGTHPEYTSEAMLDATEDYIAAGGR
HHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHCCCE
FIYLGGNGFYWNVGYHSDDPWCMEVRKLNSGMRAWQARPGEYYLATTGQKSGLWKDLGRP
EEEECCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCCCHHHHCCC
PQKIFGVGFISEGFDSARPFRRMPDSWHRRVSWIMDGIEGEIIGDFGLAQGAAGGIEIDR
HHHHEEECCHHCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
YDLTLGTPPHSLIVASSGGHSDNYQTVVEEVLYPYPGLSGSHDYRVRADMVYFTAPNDGA
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCC
VFSTGSIAFSQSLPYQNFDNNVSRLLANVVTAFSKPGKLPGWAWSAEEKQWR
EEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
AEKKVFGYADKISVKPGDDISFFVHADGTDVVDAQLVRLIHGDAHPAGPGYREEEIACE
CCCCEECCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEC
ANGVWRVRKQFTQVGSFLTVADPEQRLAPNGSFSLCIFVHPNSPGGGRRQCLLGKWDAFG
CCCHHHHHHHHHHHCCEEEECCCHHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCC
NRGYGLWLNPDGFLEFGFGDGHEVDYLDAEVPVLKNNWYFVAATFDATTGVATLYQEGVA
CCCEEEEECCCCCEEECCCCCCCEEEECCCCCEEECCEEEEEEECCCCCHHHHHHHHHHH
TRYNSLLSKVANVDFRSHVRETLRFRPVNPPDVPFLLAGARDHHTLRGDFVTQCLNGKLD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCC
RPAVFDRVLTHDELDRYRDTGLAPQNGLLAYWDTAQGYTAQGIGDRVIDVGPYGLHAEGY
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCEEEECCCCEECCCC
NRPVRAQTGFNWQGRDDCFRLAPEQYGGIEFHDDAIIDCKWELTRSIKLPDLRSGAYAFR
CCCEECCCCCCCCCCCHHHHCCHHHCCCEEECCCCEEEEEEEEECCCCCCCCCCCCEEEE
LRTGDAKGMREEYIVFFVRSVAPKAPILFLVPTGSYLAYANEHLSFDAEIMQPLAGQSPI
EECCCCCCCHHHHHEEEEHHCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCC
LSEVDIELYQTAEFGLSLYDHHADGAGVCYSTYRRPILNMRPKARMSSMGVTWQFPADLS
CHHCCEEEEEEHHHCCEEEECCCCCCCEEHHHHCCCCCCCCCCHHHHHCCEEEECCCCCH
IIAWLEHMGYDYDLTTDEDLHREGADALKPYNVVLSGTHPEYTSEAMLDATEDYIAAGGR
HHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHCCCE
FIYLGGNGFYWNVGYHSDDPWCMEVRKLNSGMRAWQARPGEYYLATTGQKSGLWKDLGRP
EEEECCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCCCHHHHCCC
PQKIFGVGFISEGFDSARPFRRMPDSWHRRVSWIMDGIEGEIIGDFGLAQGAAGGIEIDR
HHHHEEECCHHCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
YDLTLGTPPHSLIVASSGGHSDNYQTVVEEVLYPYPGLSGSHDYRVRADMVYFTAPNDGA
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCC
VFSTGSIAFSQSLPYQNFDNNVSRLLANVVTAFSKPGKLPGWAWSAEEKQWR
EEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA