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The map label for this gene is ddl [H]

Identifier: 86749124

GI number: 86749124

Start: 2273447

End: 2274436

Strand: Direct

Name: ddl [H]

Synonym: RPB_2001

Alternate gene names: 86749124

Gene position: 2273447-2274436 (Clockwise)

Preceding gene: 86749123

Following gene: 86749125

Centisome position: 42.64

GC content: 69.49

Gene sequence:

>990_bases
ATGCGACAGACAATTCTCTTCGGCGGCACCAGCAAGGAGCGCCTGGTTTCGGTCGCCTCGGCGCAGGCGCTGCACGCCGC
GCTGCCCGATGCCGAATTGTGGTTCTGGAACGACGACGACAGCGTCCACGCGGTGACCGCGGAGGCGCTGCTGGCGCATG
CGCGGCCGTTCGAGGAGGCGTTTCGGCCGGCTGGCGAAAACATCGGTCCGCTGGAGCGCGCGCTCGATCGCGCGGCGATC
GAACAGCGGCTGCTGGTGCTGGGTCTGCACGGCGGTGTCGCCGAGAACGGCGAGTTGCAGGCGATGTGCGAGATGCGCGG
CGTGCCGTTCACCGGGTCGGGCGCGGCGGCGTCGCATCTCGCCTTCGACAAGGTGGCCGCCAAGCGGTTCGCCGCGATCG
CCGGCGTGCGCGCGCCGGCCGGCATCGCGCTGGCGGAGGCCGAGGCGGCGCTGGCCGCCCACGGCCGGCTGATCGCCAAG
CCGGCCCGCGACGGGTCGAGCTACGGCCTGTTCTTCATCAATGCGAAGCAGGACCTGGTCGCGGTGCGCGACGCGGCGAG
GTCCGAGGACTATCTGATCGAGCCGTTCGTCTCCGGCATCGAAGCGACCTGCGGCGTGCTGGAGCAGGCCGACGGCTCGC
TGCTGGCGCTGCCGCCGATCGAGATCGTGCCGGCCGACGGCGGCTTCGACTACACCGCGAAATATCTCGCCAAATCGACC
CAGGAGATCTGCCCCGGCCGGTTCGCGCCGCAGATCTCGGCGAGGATCATGGAGGATGCCGTGAAGGCGCATCGGGTGAT
GGGCTGCCGCGGCTATTCGCGCTCCGACTTCATCGTCGTCGCCGACGGTCCGATCTTTCTCGAGACCAATACGCTGCCCG
GACTGACCAAGGCCTCGCTCTATCCCAAGGCGCTGCAGGCGCAGGGGATCGCCTTCGTCGATTTCCTCCACGGCCAGATC
GCGCTCGCCGAACGCGGCGCCCGGCGTTAA

Upstream 100 bases:

>100_bases
CTTCTCCGAATCAGCCAAATCGGGGAGATTGCGCCCGCGCCGGCCCGCGCGTTTCACGGACCATTCGATCGAGCAGGGAC
CCGCGCGGCGGACCGAATTC

Downstream 100 bases:

>100_bases
CGACTTTGGTCGAGTTCCCGGTTTCGGCGCTGCAAAAATGCCGCTGGCGGCCGCAAATCTTTCAGGAAACGTAACAATTC
CGGGGCCGAACCGAGCGCCT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRQTILFGGTSKERLVSVASAQALHAALPDAELWFWNDDDSVHAVTAEALLAHARPFEEAFRPAGENIGPLERALDRAAI
EQRLLVLGLHGGVAENGELQAMCEMRGVPFTGSGAAASHLAFDKVAAKRFAAIAGVRAPAGIALAEAEAALAAHGRLIAK
PARDGSSYGLFFINAKQDLVAVRDAARSEDYLIEPFVSGIEATCGVLEQADGSLLALPPIEIVPADGGFDYTAKYLAKST
QEICPGRFAPQISARIMEDAVKAHRVMGCRGYSRSDFIVVADGPIFLETNTLPGLTKASLYPKALQAQGIAFVDFLHGQI
ALAERGARR

Sequences:

>Translated_329_residues
MRQTILFGGTSKERLVSVASAQALHAALPDAELWFWNDDDSVHAVTAEALLAHARPFEEAFRPAGENIGPLERALDRAAI
EQRLLVLGLHGGVAENGELQAMCEMRGVPFTGSGAAASHLAFDKVAAKRFAAIAGVRAPAGIALAEAEAALAAHGRLIAK
PARDGSSYGLFFINAKQDLVAVRDAARSEDYLIEPFVSGIEATCGVLEQADGSLLALPPIEIVPADGGFDYTAKYLAKST
QEICPGRFAPQISARIMEDAVKAHRVMGCRGYSRSDFIVVADGPIFLETNTLPGLTKASLYPKALQAQGIAFVDFLHGQI
ALAERGARR
>Mature_329_residues
MRQTILFGGTSKERLVSVASAQALHAALPDAELWFWNDDDSVHAVTAEALLAHARPFEEAFRPAGENIGPLERALDRAAI
EQRLLVLGLHGGVAENGELQAMCEMRGVPFTGSGAAASHLAFDKVAAKRFAAIAGVRAPAGIALAEAEAALAAHGRLIAK
PARDGSSYGLFFINAKQDLVAVRDAARSEDYLIEPFVSGIEATCGVLEQADGSLLALPPIEIVPADGGFDYTAKYLAKST
QEICPGRFAPQISARIMEDAVKAHRVMGCRGYSRSDFIVVADGPIFLETNTLPGLTKASLYPKALQAQGIAFVDFLHGQI
ALAERGARR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=337, Percent_Identity=32.3442136498516, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=31.4606741573034, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34917; Mature: 34917

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50975 ATP_GRASP ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTILFGGTSKERLVSVASAQALHAALPDAELWFWNDDDSVHAVTAEALLAHARPFEEA
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCHHHH
FRPAGENIGPLERALDRAAIEQRLLVLGLHGGVAENGELQAMCEMRGVPFTGSGAAASHL
HCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEHHHHCCCCCCCCCCCHHHH
AFDKVAAKRFAAIAGVRAPAGIALAEAEAALAAHGRLIAKPARDGSSYGLFFINAKQDLV
HHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCHHH
AVRDAARSEDYLIEPFVSGIEATCGVLEQADGSLLALPPIEIVPADGGFDYTAKYLAKST
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCHHHHHHHHHH
QEICPGRFAPQISARIMEDAVKAHRVMGCRGYSRSDFIVVADGPIFLETNTLPGLTKASL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCCCCCHHHC
YPKALQAQGIAFVDFLHGQIALAERGARR
CHHHHHHCCEEEEEHHCCEEEECCCCCCC
>Mature Secondary Structure
MRQTILFGGTSKERLVSVASAQALHAALPDAELWFWNDDDSVHAVTAEALLAHARPFEEA
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCHHHH
FRPAGENIGPLERALDRAAIEQRLLVLGLHGGVAENGELQAMCEMRGVPFTGSGAAASHL
HCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEHHHHCCCCCCCCCCCHHHH
AFDKVAAKRFAAIAGVRAPAGIALAEAEAALAAHGRLIAKPARDGSSYGLFFINAKQDLV
HHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCHHH
AVRDAARSEDYLIEPFVSGIEATCGVLEQADGSLLALPPIEIVPADGGFDYTAKYLAKST
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCHHHHHHHHHH
QEICPGRFAPQISARIMEDAVKAHRVMGCRGYSRSDFIVVADGPIFLETNTLPGLTKASL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCCCCCHHHC
YPKALQAQGIAFVDFLHGQIALAERGARR
CHHHHHHCCEEEEEHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]