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The map label for this gene is emrB [H]

Identifier: 86749104

GI number: 86749104

Start: 2250567

End: 2252153

Strand: Direct

Name: emrB [H]

Synonym: RPB_1981

Alternate gene names: 86749104

Gene position: 2250567-2252153 (Clockwise)

Preceding gene: 86749103

Following gene: 86749105

Centisome position: 42.21

GC content: 64.08

Gene sequence:

>1587_bases
ATGTCGGCATCCACCACAGCGACCGCCGGAGCGGTGCCGCAGGCGCCGTCCGAACACGTGCCGGCCAGCCGGCTGATCGC
GTTCCTGATCATGGTGTTCGGGATGTTCATGTCGATCCTGGACATCCAGATCGTCTCGGCATCGCTGTCCGAAATCCAGG
CCGGCCTGTCGGCGTCATCGTCCGAAGTCTCCTGGGTGCAGACCTCGTATCTGATCGCCGAAGTGATCGCGATCCCGCTG
TCCGGCTTCCTGTCGCGCGCGCTCGGCACGCGCAATCTGTTCGCGATCTCGGCGGCCGGCTTCACCTTCGCCAGCCTGAT
GTGCGGTCTCACCTCGTCGATCACCGAGATGATCGTGTGGCGGGCGATCCAGGGCTTCCTCGGCGCCGGCATGATCCCGA
CCGTGTTCGCCTCGGCCTACACGGTGTTTCCGCGCAGCAAATTCAACCTGGTCGGGCCGATCATCGGCCTGGTGGCGACG
CTGGCGCCGACCATCGGCCCGACGGTCGGCGGCTACATCACCGACCTGATGTCGTGGCACTGGCTGTTCTTCATCAACGT
CGTCCCCGGCATCGGCATCACCATCGGCGTGCTGATGCTGGTCGATTTCGACGAGCCGCATTACGAACTGCTCGACCATT
TCGACTGGTGGGGGCTGCTGTTCATGGCGGGCTTCCTCGGCTCGCTCGAATACGTGCTCGAGGAAGGCCATCGCAACGAC
TGGTTCAGCGACGAGTCGATCTTCATCTTCGCGATCGTCTGTGCGGTCTGCGCCGTGGGGTTCTTCTGGCGGGTGCTGAC
CGCGCGCGAGCCGATCGTCGACATCCGTGCCTTCACCAACCGCAATTTCGCGTTCGGTTGCCTGTTCTCGTTCTGCGTCG
GTATCGGCCTGTACGGCCTGACCTACATCTATCCGCGCTACCTCGCCGAAGTCCGCGGCTACAGCGCGCTGATGATCGGC
GAGACGATGTTCGTCTCCGGCATCGCGATGTTTCTGTCCGCGCCGCTGGTCGGCCGGCTGATGACGCTGGTCGACATGCG
CATCCTGATCGCGATCGGCCTTTTGCTGTTCGCCGCCGGCACCTGGCTGATGACCGGGATCACCCGCGACTATGATTTCT
ACGAGCTGCTGTGGCCGCAGATCTTCCGCGGCGTCGGCATGATGATGGCGATGGTGCCGGTCAACAACATCGCGCTCGGC
ACGCTGCCGCCGGAGCGCGTCAAGAACGCCTCCGGCCTGTTCAACCTGACGCGCAATCTCGGCGGCGCGCTCGGGCTGGC
GCTGATCAACACCATCCTCGACGGCCGCACCGATTTCCACATCTCGCGGCTGCACGACAAGGTGAATTGGGGCAACGCCC
AGGCGGTCGACTTCCTCAACATGCTGACGCAGAAATTCCAGGGCATGGGCGACGCCTCGCTGATGGCGCTGAAGCAGTTC
AACCAAATCGTCCACCGCCAGGCCGTCACCATGAGCTTCGGCGACGCGTTCTTCCTGCTGACGATCTTCTACGTTGGGCT
CAGCACGCTGGTGGTGCTGGTGGCGAAACCGGCGAACCCGGCGGCAGCGGGGGGCGGGGGGCATTAG

Upstream 100 bases:

>100_bases
GCGACGAAGCAATCCAGCTCAGCGCACCGAGCCCTGGTTTGCTTCGCTTCGCTCGCAATGACGGATAGAACATTGCAGGT
TGGCCTCTAAGGAACTGCCG

Downstream 100 bases:

>100_bases
AGGCCCGTGCGAACTGCGGTGACGACGAACGGGCGCCGTGATAGACTGCAGTTATGGCATCAGACGATCACGATCAGAAG
CCGCCGGCAGAGCAGGGATT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSASTTATAGAVPQAPSEHVPASRLIAFLIMVFGMFMSILDIQIVSASLSEIQAGLSASSSEVSWVQTSYLIAEVIAIPL
SGFLSRALGTRNLFAISAAGFTFASLMCGLTSSITEMIVWRAIQGFLGAGMIPTVFASAYTVFPRSKFNLVGPIIGLVAT
LAPTIGPTVGGYITDLMSWHWLFFINVVPGIGITIGVLMLVDFDEPHYELLDHFDWWGLLFMAGFLGSLEYVLEEGHRND
WFSDESIFIFAIVCAVCAVGFFWRVLTAREPIVDIRAFTNRNFAFGCLFSFCVGIGLYGLTYIYPRYLAEVRGYSALMIG
ETMFVSGIAMFLSAPLVGRLMTLVDMRILIAIGLLLFAAGTWLMTGITRDYDFYELLWPQIFRGVGMMMAMVPVNNIALG
TLPPERVKNASGLFNLTRNLGGALGLALINTILDGRTDFHISRLHDKVNWGNAQAVDFLNMLTQKFQGMGDASLMALKQF
NQIVHRQAVTMSFGDAFFLLTIFYVGLSTLVVLVAKPANPAAAGGGGH

Sequences:

>Translated_528_residues
MSASTTATAGAVPQAPSEHVPASRLIAFLIMVFGMFMSILDIQIVSASLSEIQAGLSASSSEVSWVQTSYLIAEVIAIPL
SGFLSRALGTRNLFAISAAGFTFASLMCGLTSSITEMIVWRAIQGFLGAGMIPTVFASAYTVFPRSKFNLVGPIIGLVAT
LAPTIGPTVGGYITDLMSWHWLFFINVVPGIGITIGVLMLVDFDEPHYELLDHFDWWGLLFMAGFLGSLEYVLEEGHRND
WFSDESIFIFAIVCAVCAVGFFWRVLTAREPIVDIRAFTNRNFAFGCLFSFCVGIGLYGLTYIYPRYLAEVRGYSALMIG
ETMFVSGIAMFLSAPLVGRLMTLVDMRILIAIGLLLFAAGTWLMTGITRDYDFYELLWPQIFRGVGMMMAMVPVNNIALG
TLPPERVKNASGLFNLTRNLGGALGLALINTILDGRTDFHISRLHDKVNWGNAQAVDFLNMLTQKFQGMGDASLMALKQF
NQIVHRQAVTMSFGDAFFLLTIFYVGLSTLVVLVAKPANPAAAGGGGH
>Mature_527_residues
SASTTATAGAVPQAPSEHVPASRLIAFLIMVFGMFMSILDIQIVSASLSEIQAGLSASSSEVSWVQTSYLIAEVIAIPLS
GFLSRALGTRNLFAISAAGFTFASLMCGLTSSITEMIVWRAIQGFLGAGMIPTVFASAYTVFPRSKFNLVGPIIGLVATL
APTIGPTVGGYITDLMSWHWLFFINVVPGIGITIGVLMLVDFDEPHYELLDHFDWWGLLFMAGFLGSLEYVLEEGHRNDW
FSDESIFIFAIVCAVCAVGFFWRVLTAREPIVDIRAFTNRNFAFGCLFSFCVGIGLYGLTYIYPRYLAEVRGYSALMIGE
TMFVSGIAMFLSAPLVGRLMTLVDMRILIAIGLLLFAAGTWLMTGITRDYDFYELLWPQIFRGVGMMMAMVPVNNIALGT
LPPERVKNASGLFNLTRNLGGALGLALINTILDGRTDFHISRLHDKVNWGNAQAVDFLNMLTQKFQGMGDASLMALKQFN
QIVHRQAVTMSFGDAFFLLTIFYVGLSTLVVLVAKPANPAAAGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=511, Percent_Identity=32.0939334637965, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788710, Length=493, Percent_Identity=29.8174442190669, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081983, Length=439, Percent_Identity=27.1070615034169, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790195, Length=418, Percent_Identity=24.6411483253589, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788392, Length=443, Percent_Identity=26.86230248307, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=26.1744966442953, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57433; Mature: 57302

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASTTATAGAVPQAPSEHVPASRLIAFLIMVFGMFMSILDIQIVSASLSEIQAGLSASS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SEVSWVQTSYLIAEVIAIPLSGFLSRALGTRNLFAISAAGFTFASLMCGLTSSITEMIVW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
RAIQGFLGAGMIPTVFASAYTVFPRSKFNLVGPIIGLVATLAPTIGPTVGGYITDLMSWH
HHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WLFFINVVPGIGITIGVLMLVDFDEPHYELLDHFDWWGLLFMAGFLGSLEYVLEEGHRND
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
WFSDESIFIFAIVCAVCAVGFFWRVLTAREPIVDIRAFTNRNFAFGCLFSFCVGIGLYGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TYIYPRYLAEVRGYSALMIGETMFVSGIAMFLSAPLVGRLMTLVDMRILIAIGLLLFAAG
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TWLMTGITRDYDFYELLWPQIFRGVGMMMAMVPVNNIALGTLPPERVKNASGLFNLTRNL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCHHHHHHHHHHH
GGALGLALINTILDGRTDFHISRLHDKVNWGNAQAVDFLNMLTQKFQGMGDASLMALKQF
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NQIVHRQAVTMSFGDAFFLLTIFYVGLSTLVVLVAKPANPAAAGGGGH
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SASTTATAGAVPQAPSEHVPASRLIAFLIMVFGMFMSILDIQIVSASLSEIQAGLSASS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC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HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]