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The map label for this gene is purD [H]

Identifier: 86748986

GI number: 86748986

Start: 2127432

End: 2128760

Strand: Direct

Name: purD [H]

Synonym: RPB_1863

Alternate gene names: 86748986

Gene position: 2127432-2128760 (Clockwise)

Preceding gene: 86748985

Following gene: 86748987

Centisome position: 39.9

GC content: 68.4

Gene sequence:

>1329_bases
GTGTTACACCTCGCGCACCATCCTCGGGGTACGGTCAGGCTTGCGATGAATATTCTGTTGCTCGGCTCCGGCGGGCGCGA
ACACGCGCTGGCCTGGAAGATCGCCGCCTCCCCGCTGGTGACCAAGCTGTTTTGCGCGCCGGGCAATGCCGGGATCGCGC
GCGAGGCGACCTGCGTTGCGCTCGACGTCACCGACCATGCCGCGGTGATCGATTTCTGCAAGGCGAATGCCATCGAACTG
GTGGTGGTCGGGCCGGAGGCGCCGCTCGCCGCCGGCATCGTCGACGATCTCGCCGGCGCCGGCATCAAGGCGTTCGGCCC
GACGCAGAAGGCCGCGCAGCTCGAAGGCTCCAAGGGCTTCACCAAGGATCTGTGCAAGGCGCACGACATTCCCACCGCCG
CCTATGAGCGCTTCAGCGATCCCGCCGACGCCAAGGCCTATATCCGCGTGCAGGGCGCACCGATCGTGGTCAAGGCCGAT
GGGCTCGCGGCCGGCAAGGGCGTCGTGGTGGCGATGACGCTGGCCGAGGCCGAAGACGCGGTCGACATGATGTTCGGCGG
CGCGCTCGGCGACGCCGGCATCGAGGTCGTGGTCGAGGATTTCATGATCGGCGAGGAGGCCTCGTTCTTCGTGCTGTGCG
ACGGCGAGCACGCGCTGCCGCTCGCCACCGCGCAGGACCACAAGCGCGCCTTCGACGGCGACAAGGGGCCGAACACCGGC
GGCATGGGGGCGTATTCGCCGGCGCCGGTGATGACGGACGCGATCTGCAAGCAGGCGATGGAGCGTATCATCGGCCCGAC
GCTGAAGGCGATGAAGGCGATGGGCGCGCCGTTCAAGGGCGTGCTGTTCGCCGGGCTGATGATCACCGAGGACGGTCCGC
AACTGATCGAATACAATGTCCGCTTCGGCGATCCGGAATGCCAGGTGCTGATGATGCGGATGATGTCCGACATCGTGCCG
GCGCTGCTCGCCTGCGTCGATGGCCAACTCGCCCACCTCACGCTGCGCTGGTTCGACGAGCCGGCGCTGACCGTGGTGAT
GGCGGCGAAGGGCTATCCGGGCGGTTACGAAAAAGGCACGCGGATCGCCGGCCTCGACCGCGCCGAGCAGATCCCCGGCG
TGCAGATCTTCCACGCCGGCACGGTCGCGTCGGGCGACTGGGTGCTCGCCAATGGCGGCCGCGTGCTGGCCGTCACCGCC
TCGGCCAAGACGGTGGCCGAGGCGCAGCGCCGCGCCTACGAGGCGATCGGCGTCATCAACTGGCCCGAAGGCTTCTGCCG
CCGCGACATCGGCTGGCAGGCGGTGGCGCGGGAACGCCGAAAGAAGTAG

Upstream 100 bases:

>100_bases
GTGGCCACGCTGCGGGAAATTATCGCCGGGGCCGTGCCGGGCGGCGGCTGACGACATATCCACATCCGCGGCGACGCCCG
CGTTGCCCGGAGCCCCGGCC

Downstream 100 bases:

>100_bases
TTCAGTTGTTCGACATTCGCCCAGGCGCTGGACTGCTCCCTCTCCCGCTTGCGGGAGAGGGTTGGGGTGAGGGCGAGCCG
AGCACCGAGCCCGCGTAGTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLHLAHHPRGTVRLAMNILLLGSGGREHALAWKIAASPLVTKLFCAPGNAGIAREATCVALDVTDHAAVIDFCKANAIEL
VVVGPEAPLAAGIVDDLAGAGIKAFGPTQKAAQLEGSKGFTKDLCKAHDIPTAAYERFSDPADAKAYIRVQGAPIVVKAD
GLAAGKGVVVAMTLAEAEDAVDMMFGGALGDAGIEVVVEDFMIGEEASFFVLCDGEHALPLATAQDHKRAFDGDKGPNTG
GMGAYSPAPVMTDAICKQAMERIIGPTLKAMKAMGAPFKGVLFAGLMITEDGPQLIEYNVRFGDPECQVLMMRMMSDIVP
ALLACVDGQLAHLTLRWFDEPALTVVMAAKGYPGGYEKGTRIAGLDRAEQIPGVQIFHAGTVASGDWVLANGGRVLAVTA
SAKTVAEAQRRAYEAIGVINWPEGFCRRDIGWQAVARERRKK

Sequences:

>Translated_442_residues
MLHLAHHPRGTVRLAMNILLLGSGGREHALAWKIAASPLVTKLFCAPGNAGIAREATCVALDVTDHAAVIDFCKANAIEL
VVVGPEAPLAAGIVDDLAGAGIKAFGPTQKAAQLEGSKGFTKDLCKAHDIPTAAYERFSDPADAKAYIRVQGAPIVVKAD
GLAAGKGVVVAMTLAEAEDAVDMMFGGALGDAGIEVVVEDFMIGEEASFFVLCDGEHALPLATAQDHKRAFDGDKGPNTG
GMGAYSPAPVMTDAICKQAMERIIGPTLKAMKAMGAPFKGVLFAGLMITEDGPQLIEYNVRFGDPECQVLMMRMMSDIVP
ALLACVDGQLAHLTLRWFDEPALTVVMAAKGYPGGYEKGTRIAGLDRAEQIPGVQIFHAGTVASGDWVLANGGRVLAVTA
SAKTVAEAQRRAYEAIGVINWPEGFCRRDIGWQAVARERRKK
>Mature_442_residues
MLHLAHHPRGTVRLAMNILLLGSGGREHALAWKIAASPLVTKLFCAPGNAGIAREATCVALDVTDHAAVIDFCKANAIEL
VVVGPEAPLAAGIVDDLAGAGIKAFGPTQKAAQLEGSKGFTKDLCKAHDIPTAAYERFSDPADAKAYIRVQGAPIVVKAD
GLAAGKGVVVAMTLAEAEDAVDMMFGGALGDAGIEVVVEDFMIGEEASFFVLCDGEHALPLATAQDHKRAFDGDKGPNTG
GMGAYSPAPVMTDAICKQAMERIIGPTLKAMKAMGAPFKGVLFAGLMITEDGPQLIEYNVRFGDPECQVLMMRMMSDIVP
ALLACVDGQLAHLTLRWFDEPALTVVMAAKGYPGGYEKGTRIAGLDRAEQIPGVQIFHAGTVASGDWVLANGGRVLAVTA
SAKTVAEAQRRAYEAIGVINWPEGFCRRDIGWQAVARERRKK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=45.7943925233645, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=45.7943925233645, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=45.7943925233645, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=45.7943925233645, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=57.6744186046512, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=37.914691943128, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=41.6107382550336, Blast_Score=332, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24582400, Length=417, Percent_Identity=42.6858513189448, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI62471619, Length=417, Percent_Identity=42.6858513189448, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46561; Mature: 46561

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLAHHPRGTVRLAMNILLLGSGGREHALAWKIAASPLVTKLFCAPGNAGIAREATCVA
CCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHEEEECCCCCCCCCCCEEEE
LDVTDHAAVIDFCKANAIELVVVGPEAPLAAGIVDDLAGAGIKAFGPTQKAAQLEGSKGF
EECCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC
TKDLCKAHDIPTAAYERFSDPADAKAYIRVQGAPIVVKADGLAAGKGVVVAMTLAEAEDA
HHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEEEEEHHHHHH
VDMMFGGALGDAGIEVVVEDFMIGEEASFFVLCDGEHALPLATAQDHKRAFDGDKGPNTG
HHHHHCCCCCCCCHHEEEEHHHCCCCCCEEEEECCCCCCEECCCHHHHHHCCCCCCCCCC
GMGAYSPAPVMTDAICKQAMERIIGPTLKAMKAMGAPFKGVLFAGLMITEDGPQLIEYNV
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEEEEE
RFGDPECQVLMMRMMSDIVPALLACVDGQLAHLTLRWFDEPALTVVMAAKGYPGGYEKGT
EECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCC
RIAGLDRAEQIPGVQIFHAGTVASGDWVLANGGRVLAVTASAKTVAEAQRRAYEAIGVIN
EEECCCHHHHCCCEEEEECCEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCEEE
WPEGFCRRDIGWQAVARERRKK
CCHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLHLAHHPRGTVRLAMNILLLGSGGREHALAWKIAASPLVTKLFCAPGNAGIAREATCVA
CCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHEEEECCCCCCCCCCCEEEE
LDVTDHAAVIDFCKANAIELVVVGPEAPLAAGIVDDLAGAGIKAFGPTQKAAQLEGSKGF
EECCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC
TKDLCKAHDIPTAAYERFSDPADAKAYIRVQGAPIVVKADGLAAGKGVVVAMTLAEAEDA
HHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEEEEEHHHHHH
VDMMFGGALGDAGIEVVVEDFMIGEEASFFVLCDGEHALPLATAQDHKRAFDGDKGPNTG
HHHHHCCCCCCCCHHEEEEHHHCCCCCCEEEEECCCCCCEECCCHHHHHHCCCCCCCCCC
GMGAYSPAPVMTDAICKQAMERIIGPTLKAMKAMGAPFKGVLFAGLMITEDGPQLIEYNV
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEEEEE
RFGDPECQVLMMRMMSDIVPALLACVDGQLAHLTLRWFDEPALTVVMAAKGYPGGYEKGT
EECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCC
RIAGLDRAEQIPGVQIFHAGTVASGDWVLANGGRVLAVTASAKTVAEAQRRAYEAIGVIN
EEECCCHHHHCCCEEEEECCEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCEEE
WPEGFCRRDIGWQAVARERRKK
CCHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]