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The map label for this gene is pmbA [H]

Identifier: 86748975

GI number: 86748975

Start: 2117661

End: 2119058

Strand: Direct

Name: pmbA [H]

Synonym: RPB_1852

Alternate gene names: 86748975

Gene position: 2117661-2119058 (Clockwise)

Preceding gene: 86748973

Following gene: 86748976

Centisome position: 39.72

GC content: 69.31

Gene sequence:

>1398_bases
GTGAACTCTTCACCAAGCATTCGATCGCCGTCGTCGCCTTCCAAGGATCAGGACCTGTTCGATCAGTCGGCGTTGAGCGA
ACTGGCGCAGCGGCTGGTCGAGGCGGCGTTGCGCGCCGGCGCCGATCAGGCCGACGCGGTCGCGGTGCGCGGCGTCTCGC
ACGGCGTCGAACTACGCGATGGACGGATGCAGGAATCCGAGCGCTCCGAGGGCGACGATGTCGGCCTGCGCGTGCTGGTC
GGCCGCCGCCAGGCGGTGGTCTCGACCAACGATATCACCGGCGACGGCGTCTCGAAACTGGCCGAGCGCGCGGTGGCGAT
GGCCCGGGTCGCGCCCGACGACAAATATGTCGGCCTCGCCGATCCCGCGCTGCTCGCCCGCGATTTTCCCGATCTCGACA
TGCTCGATCCGCAGACGCCCTCGACCGCCGAGCTGGAGCGTCGCGCGCGCGAAGCCGAAGCCGCGGCGCTGGTGGTGAAG
GGTGTCACCAAATCGGGCGGCGCCTCGGCATCGGCGGGCATCGGCGGCATGGTGCTGGTGACCTCGACCGGCTTCCACGG
CGCCTTCTTGCGCTCCAGCCAGAGCGTCTCGATGACCGCGATCGCCGGCGACGGCACCGGCATGGAGCGCGACTACGAAT
ACACCACGGCGCCGCACGCCGCGGATCTGATGTCGCCGGAGACCGTCGGCCGCGTCGCCGGCGAGCGCACCGCGGCGCGC
GTCGGCCCGCGCAAGGTCGAGACCTGCAAGGTGCCGGTGATCTTCGATCCGCGCGTCGCCGGCTCGCTGGTCGGGCATCT
GGTCGGCGCGGTCAATGGCGCCGCGATCGCGCGCAAGACCTCGTTCCTGAAGGATCGGCTCGGCGACAAGCTGTTTCGCG
ACGGCATCCGCATCGTCGACGATCCGCTGCGCAAGCGCGGGCTGCGCTCGCAGGCGTTCGACGCCGAGGGCGTCGCGGTG
AAAGAGCTTGCGATCGTCGATGACGGCGTGCTGACCAGCTGGCTGCTCGACTGCGCCACCGCGCGCGAACTCGGGCTCAC
CACCACCGGCCACGCGCATCGCGGCGTGTCGTCGTCGCCGTCGCCCGGGCCCTACAATCTGCATCTCGAAGGCGGCACGA
TGAGCCGGGCCGAGCTGATGAGCGACATCAAGCAGGGTTTCTACGTCACCGACCTGATCGGCTCCGGCGTCAACGGCGTC
ACCGGCGACTACAGCCGCGGCGCCGCCGGGTTCTGGATCGAGAACGGCGAGTGCACCTACGCGGTCAGCGAGGTCACCAT
CGCCGGCCATCTGATCGAGATGTACAAGACGCTGACGCCGGCCAATGATCTGACCTTCCGGTATGGCGTCAACGCGCCGA
CGCTGCGGATCGAGGGGCTGACGATTGCCGGACGCTAG

Upstream 100 bases:

>100_bases
GCAAAGTGCTTGCAGTCCGCGCCGAGCAGGCACATCTGTGAAATCTCGGGATGCGTTTCCGGGCTCGCCGTCATTGCTTT
CAGCCTCAGGATTTTTGTTT

Downstream 100 bases:

>100_bases
TCCCGATACGCGACACAGCGGGGCCGGGTCGCGCGACGCGATGCTGCTGGCCGAGACGGTCCGGGACGCCGGTGGCTTGG
CGCTGTCGATGTTCCGCACC

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNSSPSIRSPSSPSKDQDLFDQSALSELAQRLVEAALRAGADQADAVAVRGVSHGVELRDGRMQESERSEGDDVGLRVLV
GRRQAVVSTNDITGDGVSKLAERAVAMARVAPDDKYVGLADPALLARDFPDLDMLDPQTPSTAELERRAREAEAAALVVK
GVTKSGGASASAGIGGMVLVTSTGFHGAFLRSSQSVSMTAIAGDGTGMERDYEYTTAPHAADLMSPETVGRVAGERTAAR
VGPRKVETCKVPVIFDPRVAGSLVGHLVGAVNGAAIARKTSFLKDRLGDKLFRDGIRIVDDPLRKRGLRSQAFDAEGVAV
KELAIVDDGVLTSWLLDCATARELGLTTTGHAHRGVSSSPSPGPYNLHLEGGTMSRAELMSDIKQGFYVTDLIGSGVNGV
TGDYSRGAAGFWIENGECTYAVSEVTIAGHLIEMYKTLTPANDLTFRYGVNAPTLRIEGLTIAGR

Sequences:

>Translated_465_residues
MNSSPSIRSPSSPSKDQDLFDQSALSELAQRLVEAALRAGADQADAVAVRGVSHGVELRDGRMQESERSEGDDVGLRVLV
GRRQAVVSTNDITGDGVSKLAERAVAMARVAPDDKYVGLADPALLARDFPDLDMLDPQTPSTAELERRAREAEAAALVVK
GVTKSGGASASAGIGGMVLVTSTGFHGAFLRSSQSVSMTAIAGDGTGMERDYEYTTAPHAADLMSPETVGRVAGERTAAR
VGPRKVETCKVPVIFDPRVAGSLVGHLVGAVNGAAIARKTSFLKDRLGDKLFRDGIRIVDDPLRKRGLRSQAFDAEGVAV
KELAIVDDGVLTSWLLDCATARELGLTTTGHAHRGVSSSPSPGPYNLHLEGGTMSRAELMSDIKQGFYVTDLIGSGVNGV
TGDYSRGAAGFWIENGECTYAVSEVTIAGHLIEMYKTLTPANDLTFRYGVNAPTLRIEGLTIAGR
>Mature_465_residues
MNSSPSIRSPSSPSKDQDLFDQSALSELAQRLVEAALRAGADQADAVAVRGVSHGVELRDGRMQESERSEGDDVGLRVLV
GRRQAVVSTNDITGDGVSKLAERAVAMARVAPDDKYVGLADPALLARDFPDLDMLDPQTPSTAELERRAREAEAAALVVK
GVTKSGGASASAGIGGMVLVTSTGFHGAFLRSSQSVSMTAIAGDGTGMERDYEYTTAPHAADLMSPETVGRVAGERTAAR
VGPRKVETCKVPVIFDPRVAGSLVGHLVGAVNGAAIARKTSFLKDRLGDKLFRDGIRIVDDPLRKRGLRSQAFDAEGVAV
KELAIVDDGVLTSWLLDCATARELGLTTTGHAHRGVSSSPSPGPYNLHLEGGTMSRAELMSDIKQGFYVTDLIGSGVNGV
TGDYSRGAAGFWIENGECTYAVSEVTIAGHLIEMYKTLTPANDLTFRYGVNAPTLRIEGLTIAGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=449, Percent_Identity=36.9710467706013, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1789640, Length=234, Percent_Identity=26.4957264957265, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48970; Mature: 48970

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSPSIRSPSSPSKDQDLFDQSALSELAQRLVEAALRAGADQADAVAVRGVSHGVELRD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECC
GRMQESERSEGDDVGLRVLVGRRQAVVSTNDITGDGVSKLAERAVAMARVAPDDKYVGLA
CCCCHHHCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCC
DPALLARDFPDLDMLDPQTPSTAELERRAREAEAAALVVKGVTKSGGASASAGIGGMVLV
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEE
TSTGFHGAFLRSSQSVSMTAIAGDGTGMERDYEYTTAPHAADLMSPETVGRVAGERTAAR
EECCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
VGPRKVETCKVPVIFDPRVAGSLVGHLVGAVNGAAIARKTSFLKDRLGDKLFRDGIRIVD
CCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHC
DPLRKRGLRSQAFDAEGVAVKELAIVDDGVLTSWLLDCATARELGLTTTGHAHRGVSSSP
CHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPGPYNLHLEGGTMSRAELMSDIKQGFYVTDLIGSGVNGVTGDYSRGAAGFWIENGECTY
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEHHHCCCCCCCCCCCCCCCCEEEEECCCEEE
AVSEVTIAGHLIEMYKTLTPANDLTFRYGVNAPTLRIEGLTIAGR
EEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECEEECCC
>Mature Secondary Structure
MNSSPSIRSPSSPSKDQDLFDQSALSELAQRLVEAALRAGADQADAVAVRGVSHGVELRD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECC
GRMQESERSEGDDVGLRVLVGRRQAVVSTNDITGDGVSKLAERAVAMARVAPDDKYVGLA
CCCCHHHCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCC
DPALLARDFPDLDMLDPQTPSTAELERRAREAEAAALVVKGVTKSGGASASAGIGGMVLV
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEE
TSTGFHGAFLRSSQSVSMTAIAGDGTGMERDYEYTTAPHAADLMSPETVGRVAGERTAAR
EECCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
VGPRKVETCKVPVIFDPRVAGSLVGHLVGAVNGAAIARKTSFLKDRLGDKLFRDGIRIVD
CCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHC
DPLRKRGLRSQAFDAEGVAVKELAIVDDGVLTSWLLDCATARELGLTTTGHAHRGVSSSP
CHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPGPYNLHLEGGTMSRAELMSDIKQGFYVTDLIGSGVNGVTGDYSRGAAGFWIENGECTY
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEHHHCCCCCCCCCCCCCCCCEEEEECCCEEE
AVSEVTIAGHLIEMYKTLTPANDLTFRYGVNAPTLRIEGLTIAGR
EEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]