Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 86748943

GI number: 86748943

Start: 2082838

End: 2084289

Strand: Direct

Name: nylA [H]

Synonym: RPB_1820

Alternate gene names: 86748943

Gene position: 2082838-2084289 (Clockwise)

Preceding gene: 86748942

Following gene: 86748947

Centisome position: 39.07

GC content: 67.91

Gene sequence:

>1452_bases
ATGGCTCTGACATTGGACGAATATGCGGCGCAGGACGGCCTCGGGCTCGCCGCGCTGGTTCGCAGCGGCGACGTCAGCGC
CGCCGAATTGGCCGCCACGGCGCAGCGGGCGATCGCGGCGCTCAATCCCACCATCAATGCCGTCATCGGCGAGGTGCCCG
CAGCGTTGCATGCGCCGGCTGCCGAAACCGAGGCCGCTTCTTTTCACGGCGTTCCGTTCCTGATCAAGGATCTGGTGATG
CACGCCGAAGGCGTCGCCTGCGATATGGGGAGCCGCTTCGTGCACGGCAGTTTCGTCTCCCCGCACGACACCGAACTGAT
GCGGCGCTTCAAGGCCGCCGGGCTGATGACGCTCGGCCGCACCAACACGCCCGAGATGGGCTTCAGCATTTCGACTGAGC
CGGTGCTGTATGGCCCGACCCGCAATCCGTGGGACCTGACCCGCTCGGCCGGAGGCTCCAGCGGAGGATCGGCCGCAGCA
GTGGCCGCCGGTCTCGTCCCAGTGGCGCATGCCAATGACGGCGGCGGGTCGATCCGTATCCCGGCAGCCTGTTGCGGCGT
GGTCGGCCTCAAGCCGACGCGCGGCCGGACGCCCACCGGGCCCGAATTCGGCCAACCGCTGAAAGGCATGGGCATCGAGC
ATGTGGTCACCCGCACGGTGCGCGATTGCGCCGCGCTGCTCGATGCGGTGCAGGGGCCCGGCGTCGGCGATCCGTTCGAA
ATTCCGCCGCCGGCGCGTCCCTATCTGCAGGAGGTCGGCGTCGAGCCGGGCCGGCTGCGGATCGCCTTCTCGCTCGCCGG
CGTGATGCAGGCCAAGATCGACCCGGAGATGCGCGACGCGTTGCTTCAGGTGGCCAAGCATCTCGAGCAGCTCGGCCATC
ACGTCGAAGAAGCTTCGCCGGTGTTCGACGAGGCGCAGTTCCACACCGCCAACCTGACCTATTGGTGCGGCTTCCTTGCC
GCTGGCATCCTTGGCGTGGGTCAGTTCAACGGCCGGACGCCGTCGTCGGAGCTGCTCGAAGCGACGACGCTGGCTTGCTA
CGAGCGCGGCCTCGCTCTGACGTTGGTCGACGGCGAAATCGCCGACGCCTTCACCAACATGGTGTGCCGCAGCGTCGCTC
CGTTCTTCCGCCAATACGACGTCCTGCTGACGCCGACGATGGCGGCTGCGGCGCTCCCGCTCGGCTTCGCCAATGGCGAC
GACGCCTCGCTGGGCGCACAGGGCTGGTACGATCGTCTGTTCCGCCACGCGCCGTTCACCGCGCTCTACAACATGACTGG
CCAGCCCGCGATCAGCCTGCCACTCTGTTCCGATCGCGAAGGGCGTCCGCTCGGCATGCAGTTCGTCGCGCGTTTCGGCG
CTGAGGACCTGCTGATCCGGCTCGCGGCGCAGCTCGAGCAATCAATGCCCTGGAAGCAGCGACGGCCCGGCGTTTACGTT
GGAGCGGTGTGA

Upstream 100 bases:

>100_bases
CCCTCGGCGGGTGGAACATCTGACCGATAGTCCCGCGGGTCCTTCTGCCTAGCATTCGCAAGAAGCTGCCAAGGCGGCGG
CGATTTGCGGAGGCGAGGGA

Downstream 100 bases:

>100_bases
CGCGACTAGGTCGGTTGGACCGGGGCCGAAAGGTCCCGGCCTTCGCATCGCGTGTGGGTCACGATTTCGATTTGCCGCCC
GCGCGGAACAGATCCATCAC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MALTLDEYAAQDGLGLAALVRSGDVSAAELAATAQRAIAALNPTINAVIGEVPAALHAPAAETEAASFHGVPFLIKDLVM
HAEGVACDMGSRFVHGSFVSPHDTELMRRFKAAGLMTLGRTNTPEMGFSISTEPVLYGPTRNPWDLTRSAGGSSGGSAAA
VAAGLVPVAHANDGGGSIRIPAACCGVVGLKPTRGRTPTGPEFGQPLKGMGIEHVVTRTVRDCAALLDAVQGPGVGDPFE
IPPPARPYLQEVGVEPGRLRIAFSLAGVMQAKIDPEMRDALLQVAKHLEQLGHHVEEASPVFDEAQFHTANLTYWCGFLA
AGILGVGQFNGRTPSSELLEATTLACYERGLALTLVDGEIADAFTNMVCRSVAPFFRQYDVLLTPTMAAAALPLGFANGD
DASLGAQGWYDRLFRHAPFTALYNMTGQPAISLPLCSDREGRPLGMQFVARFGAEDLLIRLAAQLEQSMPWKQRRPGVYV
GAV

Sequences:

>Translated_483_residues
MALTLDEYAAQDGLGLAALVRSGDVSAAELAATAQRAIAALNPTINAVIGEVPAALHAPAAETEAASFHGVPFLIKDLVM
HAEGVACDMGSRFVHGSFVSPHDTELMRRFKAAGLMTLGRTNTPEMGFSISTEPVLYGPTRNPWDLTRSAGGSSGGSAAA
VAAGLVPVAHANDGGGSIRIPAACCGVVGLKPTRGRTPTGPEFGQPLKGMGIEHVVTRTVRDCAALLDAVQGPGVGDPFE
IPPPARPYLQEVGVEPGRLRIAFSLAGVMQAKIDPEMRDALLQVAKHLEQLGHHVEEASPVFDEAQFHTANLTYWCGFLA
AGILGVGQFNGRTPSSELLEATTLACYERGLALTLVDGEIADAFTNMVCRSVAPFFRQYDVLLTPTMAAAALPLGFANGD
DASLGAQGWYDRLFRHAPFTALYNMTGQPAISLPLCSDREGRPLGMQFVARFGAEDLLIRLAAQLEQSMPWKQRRPGVYV
GAV
>Mature_482_residues
ALTLDEYAAQDGLGLAALVRSGDVSAAELAATAQRAIAALNPTINAVIGEVPAALHAPAAETEAASFHGVPFLIKDLVMH
AEGVACDMGSRFVHGSFVSPHDTELMRRFKAAGLMTLGRTNTPEMGFSISTEPVLYGPTRNPWDLTRSAGGSSGGSAAAV
AAGLVPVAHANDGGGSIRIPAACCGVVGLKPTRGRTPTGPEFGQPLKGMGIEHVVTRTVRDCAALLDAVQGPGVGDPFEI
PPPARPYLQEVGVEPGRLRIAFSLAGVMQAKIDPEMRDALLQVAKHLEQLGHHVEEASPVFDEAQFHTANLTYWCGFLAA
GILGVGQFNGRTPSSELLEATTLACYERGLALTLVDGEIADAFTNMVCRSVAPFFRQYDVLLTPTMAAAALPLGFANGDD
ASLGAQGWYDRLFRHAPFTALYNMTGQPAISLPLCSDREGRPLGMQFVARFGAEDLLIRLAAQLEQSMPWKQRRPGVYVG
AV

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=513, Percent_Identity=23.5867446393762, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI195972892, Length=511, Percent_Identity=22.3091976516634, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI166795287, Length=228, Percent_Identity=30.2631578947368, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=508, Percent_Identity=24.8031496062992, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=292, Percent_Identity=29.7945205479452, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=237, Percent_Identity=29.957805907173, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=27.9220779220779, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=27.9220779220779, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=26.6, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=24.8995983935743, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648113, Length=470, Percent_Identity=30.6382978723404, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550774, Length=501, Percent_Identity=23.1536926147705, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161078093, Length=306, Percent_Identity=28.7581699346405, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648435, Length=501, Percent_Identity=23.1536926147705, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648437, Length=501, Percent_Identity=23.1536926147705, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652985, Length=325, Percent_Identity=26.4615384615385, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922090, Length=325, Percent_Identity=26.4615384615385, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652981, Length=325, Percent_Identity=26.4615384615385, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652983, Length=325, Percent_Identity=26.4615384615385, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648441, Length=438, Percent_Identity=23.972602739726, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=438, Percent_Identity=23.972602739726, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 50923; Mature: 50792

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLDEYAAQDGLGLAALVRSGDVSAAELAATAQRAIAALNPTINAVIGEVPAALHAPA
CEEECHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHCCCC
AETEAASFHGVPFLIKDLVMHAEGVACDMGSRFVHGSFVSPHDTELMRRFKAAGLMTLGR
CCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEECCCCCCCCHHHHHHHHHHHCCEEECC
TNTPEMGFSISTEPVLYGPTRNPWDLTRSAGGSSGGSAAAVAAGLVPVAHANDGGGSIRI
CCCCCCCCEECCCCEEECCCCCCHHHHHCCCCCCCCCHHHHHHCCEEEEECCCCCCEEEC
PAACCGVVGLKPTRGRTPTGPEFGQPLKGMGIEHVVTRTVRDCAALLDAVQGPGVGDPFE
CHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IPPPARPYLQEVGVEPGRLRIAFSLAGVMQAKIDPEMRDALLQVAKHLEQLGHHVEEASP
CCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VFDEAQFHTANLTYWCGFLAAGILGVGQFNGRTPSSELLEATTLACYERGLALTLVDGEI
CHHCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHH
ADAFTNMVCRSVAPFFRQYDVLLTPTMAAAALPLGFANGDDASLGAQGWYDRLFRHAPFT
HHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHH
ALYNMTGQPAISLPLCSDREGRPLGMQFVARFGAEDLLIRLAAQLEQSMPWKQRRPGVYV
HHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCCCCEEE
GAV
ECC
>Mature Secondary Structure 
ALTLDEYAAQDGLGLAALVRSGDVSAAELAATAQRAIAALNPTINAVIGEVPAALHAPA
EEECHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHCCCC
AETEAASFHGVPFLIKDLVMHAEGVACDMGSRFVHGSFVSPHDTELMRRFKAAGLMTLGR
CCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEECCCCCCCCHHHHHHHHHHHCCEEECC
TNTPEMGFSISTEPVLYGPTRNPWDLTRSAGGSSGGSAAAVAAGLVPVAHANDGGGSIRI
CCCCCCCCEECCCCEEECCCCCCHHHHHCCCCCCCCCHHHHHHCCEEEEECCCCCCEEEC
PAACCGVVGLKPTRGRTPTGPEFGQPLKGMGIEHVVTRTVRDCAALLDAVQGPGVGDPFE
CHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IPPPARPYLQEVGVEPGRLRIAFSLAGVMQAKIDPEMRDALLQVAKHLEQLGHHVEEASP
CCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VFDEAQFHTANLTYWCGFLAAGILGVGQFNGRTPSSELLEATTLACYERGLALTLVDGEI
CHHCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHH
ADAFTNMVCRSVAPFFRQYDVLLTPTMAAAALPLGFANGDDASLGAQGWYDRLFRHAPFT
HHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHH
ALYNMTGQPAISLPLCSDREGRPLGMQFVARFGAEDLLIRLAAQLEQSMPWKQRRPGVYV
HHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCCCCEEE
GAV
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]