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The map label for this gene is tilS [H]

Identifier: 86748940

GI number: 86748940

Start: 2078428

End: 2079468

Strand: Direct

Name: tilS [H]

Synonym: RPB_1817

Alternate gene names: 86748940

Gene position: 2078428-2079468 (Clockwise)

Preceding gene: 86748939

Following gene: 86748941

Centisome position: 38.98

GC content: 70.89

Gene sequence:

>1041_bases
ATGTCCGGGACTGACGAAGCGCCGATTTCGGCGCGCGAGGCTTCGAGCCTGTTCGCCGATTGGAAGGCGGTGCCTGCGAT
CGTGCTCGCGGTCTCCGGCGGGCCGGACTCGCTGGCGCTGATGTGGCTCGCCGCGCGCTGGCGCAAGGCGCGCAAGCGCG
GGCCCGCGCTGTTCGTCGTCACCATCGACCACGGCCTGCGGCCGGAAGCGGCCCGCGAGGCGCGGTCGGTCAAGCAGTTC
GCGCGCACGCTCGGTCTGCCGCACCGGACGCTGCGCTGGACCGGCGACAAGCCCGCGACGGGCCTGCCGGCGGCGGCGCG
CGCGGCGCGCTATCGGCTGCTGGCCAAGGCGGCGAAAGCGGTCGGCGCGGCGCATATCATGACCGCGCACACCCGCGACG
ATCAGGCCGAGACCGTGCTGATGCGGCTGTCCCGCGGCAGCGGCATCGCCGGCCTGGCCGCGATGCCGCGCGAGACGGCA
CGCGACGGCGCCGTGCTGGTCCGTCCGTTGCTCGACGTGTCGAAACAGCGCCTGGTGGCGACGCTCGACAGGGCGAAGAT
CGGATTCGCGGTCGACCCGAGCAATGCCGATCCCCGGTTCACCCGCCCCCGTCTGCGCGAATTGATGCCGCTGCTGGCCG
CCGAGGGATGCGATTCGCGCAATTTGGCGCGGCTCGCCGCCCGCGCCGCCCGCGCCGAAGCCGCGCTGGAGCTGCTGGTC
GATGGCGCGGAGCGATTTCTGGCGGTGCGCAGCGCGGGCCATCCGCCACAGAGCTTCGATGCCTCGGTATTCTACGCGTT
AAACGCGGAAATCCGGATTCGGTTGTTGCTACGTGCGATCGGCCGCGTCGGCCGGGAAGGCCGGCCTGAACTCGGCAAGG
TCGAGGCATTGGCCGCCGCGCTTCAATCGGCCGCCGCCAAGCCGGCCGCCAAACCGGGGCAAATCAAGCTGAAACAAACG
CTTGCCGGGGCCATCATCACTTTGACACCAGTTTGCCTGAAGTTTGCCGCAGCGCCGATGCGGCGGCGGAAGACGGGCTG
A

Upstream 100 bases:

>100_bases
CGGCGTGAAGAAGGCGGTCGACACCGAGCAGAAGAAGCTTAAGTGCTAGGCTGACGAGAGCCCCGGCCGCGCCGGGCTCG
ATTCGAGGCTTGCGCTGCCG

Downstream 100 bases:

>100_bases
TCCCGATCGCATTTGCGTTCCGGCGGCTGTCTCATCGGCCGAACTCTCTGTGATTTGAGCCGCGGGAGCGCCGAAATGCG
CTAAATAGTCCTGAACGGTT

Product: tRNA(Ile)-lysidine synthetase-like

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSGTDEAPISAREASSLFADWKAVPAIVLAVSGGPDSLALMWLAARWRKARKRGPALFVVTIDHGLRPEAAREARSVKQF
ARTLGLPHRTLRWTGDKPATGLPAAARAARYRLLAKAAKAVGAAHIMTAHTRDDQAETVLMRLSRGSGIAGLAAMPRETA
RDGAVLVRPLLDVSKQRLVATLDRAKIGFAVDPSNADPRFTRPRLRELMPLLAAEGCDSRNLARLAARAARAEAALELLV
DGAERFLAVRSAGHPPQSFDASVFYALNAEIRIRLLLRAIGRVGREGRPELGKVEALAAALQSAAAKPAAKPGQIKLKQT
LAGAIITLTPVCLKFAAAPMRRRKTG

Sequences:

>Translated_346_residues
MSGTDEAPISAREASSLFADWKAVPAIVLAVSGGPDSLALMWLAARWRKARKRGPALFVVTIDHGLRPEAAREARSVKQF
ARTLGLPHRTLRWTGDKPATGLPAAARAARYRLLAKAAKAVGAAHIMTAHTRDDQAETVLMRLSRGSGIAGLAAMPRETA
RDGAVLVRPLLDVSKQRLVATLDRAKIGFAVDPSNADPRFTRPRLRELMPLLAAEGCDSRNLARLAARAARAEAALELLV
DGAERFLAVRSAGHPPQSFDASVFYALNAEIRIRLLLRAIGRVGREGRPELGKVEALAAALQSAAAKPAAKPGQIKLKQT
LAGAIITLTPVCLKFAAAPMRRRKTG
>Mature_345_residues
SGTDEAPISAREASSLFADWKAVPAIVLAVSGGPDSLALMWLAARWRKARKRGPALFVVTIDHGLRPEAAREARSVKQFA
RTLGLPHRTLRWTGDKPATGLPAAARAARYRLLAKAAKAVGAAHIMTAHTRDDQAETVLMRLSRGSGIAGLAAMPRETAR
DGAVLVRPLLDVSKQRLVATLDRAKIGFAVDPSNADPRFTRPRLRELMPLLAAEGCDSRNLARLAARAARAEAALELLVD
GAERFLAVRSAGHPPQSFDASVFYALNAEIRIRLLLRAIGRVGREGRPELGKVEALAAALQSAAAKPAAKPGQIKLKQTL
AGAIITLTPVCLKFAAAPMRRRKTG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=187, Percent_Identity=33.6898395721925, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36971; Mature: 36839

Theoretical pI: Translated: 11.84; Mature: 11.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTDEAPISAREASSLFADWKAVPAIVLAVSGGPDSLALMWLAARWRKARKRGPALFVV
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEE
TIDHGLRPEAAREARSVKQFARTLGLPHRTLRWTGDKPATGLPAAARAARYRLLAKAAKA
EECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
VGAAHIMTAHTRDDQAETVLMRLSRGSGIAGLAAMPRETARDGAVLVRPLLDVSKQRLVA
HHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
TLDRAKIGFAVDPSNADPRFTRPRLRELMPLLAAEGCDSRNLARLAARAARAEAALELLV
HHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DGAERFLAVRSAGHPPQSFDASVFYALNAEIRIRLLLRAIGRVGREGRPELGKVEALAAA
HHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LQSAAAKPAAKPGQIKLKQTLAGAIITLTPVCLKFAAAPMRRRKTG
HHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGTDEAPISAREASSLFADWKAVPAIVLAVSGGPDSLALMWLAARWRKARKRGPALFVV
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEE
TIDHGLRPEAAREARSVKQFARTLGLPHRTLRWTGDKPATGLPAAARAARYRLLAKAAKA
EECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
VGAAHIMTAHTRDDQAETVLMRLSRGSGIAGLAAMPRETARDGAVLVRPLLDVSKQRLVA
HHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
TLDRAKIGFAVDPSNADPRFTRPRLRELMPLLAAEGCDSRNLARLAARAARAEAALELLV
HHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DGAERFLAVRSAGHPPQSFDASVFYALNAEIRIRLLLRAIGRVGREGRPELGKVEALAAA
HHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LQSAAAKPAAKPGQIKLKQTLAGAIITLTPVCLKFAAAPMRRRKTG
HHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA