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The map label for this gene is tolB

Identifier: 86748935

GI number: 86748935

Start: 2071433

End: 2072782

Strand: Direct

Name: tolB

Synonym: RPB_1812

Alternate gene names: 86748935

Gene position: 2071433-2072782 (Clockwise)

Preceding gene: 86748934

Following gene: 86748936

Centisome position: 38.85

GC content: 66.96

Gene sequence:

>1350_bases
ATGGATTGCCCGAACATGCCGCTCCACATCAATCGTAGACAAATGCTCCTCTCCGCCGCGACTGCCGCGGGCGCGCTGGC
GCTCGGACCGGCGCGCGACGCGTTCGGCCAGGCCCGCGTGCAGATCACCGAGGGCAACGTCGCGCCGCTGCCGATCGCGA
TCCCGAATTTCGTCGCCGGCACGCCGTCGGACAATGAAGTCGGCGGCGGCGTCGCCCAGGTCATCACCAACAATCTCAAG
CGCAGCGGCCTGTTCGCGCCGATCGACCAGGCCGCCTATGTCGAGAAGATCACCAACATCGACGTGCCGCCGCAATTCAA
GAGCTGGACCAGCATCAACGCCCAGGCGCTGGTGACCGGCCGGATGACGCGGCAGTCGGACGGGCGGCTGAAGGCCGAAT
TCCGGCTGTGGGACGTCGCCACCGGCCAGCAGCTCGCCGGCCAGCAATACTTCACCTCGCCGGAATATTGGCGCCGCATC
GCCCACATCATTTCCGATCAGATCTACGAGCGGCTGACCGGCGAGAAGGGTTACTTCGACAGCCGCGTGGTGTTCATCGA
CGAGAGCGGCCCGGCGGACCGCCGCGTCAAGCGGCTGGCGCTGATGGATCAGGACGGCGCCAATGTCCGCTACCTCACGC
GCGGCAGCGACCTGGTGCTGACGCCGCGGTTCTCGCCGTCGACCCAGGAAATCACCTATATGGAGTTCGGCCAGGGCGAC
CCGAAGGTCTATCTGTTCAACATCGAGACCGGGCAGCGCGAGATCGTCGGCAATTTCCCCGGCATGTCGTTTGCGCCGCG
CTTCTCGCCGGACGGCCAGCGCATCATCATGAGCCTGCAGCAGGGCGGCAATTCGAACCTGTTCGTGATGGATCTCCGCT
CCAAATCGACGACGCGGCTGACCGACACGCCGGCGATCGACACCTCGCCGTCCTACTCGCCCGACGCCGCGCGGATCTGC
TTCGAGTCCGATCGCGGCGGCAAGCCGCAGATCTATGTGATGCCGGCCAGCGGCGGGCAGGCGCAGCGCATCTCGTTCGG
CGACGGCAGCTACTCGACCCCGGTGTGGTCGCCGCGCGGCGACTACATCGCCTTCACCAAGCAGGGCGGCGGTCAGTTCG
CGATCGGCATCATGAAGCCGGACGGCTCCGGCGAGCGCATCCTGACCTCGGGCTTCCACAACGAAGGGCCGACCTTCGCC
CCCAACGGTCGGGTGCTGATGTTCTTCCGCGATCCCGGCGGCAATGCCGGCCCGTCGCTGTTCACCGTCGACGTCTCCGG
GCGCAACGAACTGCGCGTTCCGACGCCCGGCTACGCCTCGGACCCGGCCTGGTCGCCGCTGCTGTCGTAA

Upstream 100 bases:

>100_bases
AATGGAAATACTTCGCGCCCGTGTTCACCGACCGGCGGACCTGATGCGAAGCTTGCTGACGATCGCCGGCGCTCCGCCGC
GTGCGACATCCGACTGACCG

Downstream 100 bases:

>100_bases
ACGTTAAGGTTTTCTTGACGTCTTCACGCCGGCCGTTTCGGTGTTCGGGAGCGCGATCGTAACGCCCTAAAAAGCCGAAG
TATTTTGGGGTTTCGCGGAG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MDCPNMPLHINRRQMLLSAATAAGALALGPARDAFGQARVQITEGNVAPLPIAIPNFVAGTPSDNEVGGGVAQVITNNLK
RSGLFAPIDQAAYVEKITNIDVPPQFKSWTSINAQALVTGRMTRQSDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRI
AHIISDQIYERLTGEKGYFDSRVVFIDESGPADRRVKRLALMDQDGANVRYLTRGSDLVLTPRFSPSTQEITYMEFGQGD
PKVYLFNIETGQREIVGNFPGMSFAPRFSPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRLTDTPAIDTSPSYSPDAARIC
FESDRGGKPQIYVMPASGGQAQRISFGDGSYSTPVWSPRGDYIAFTKQGGGQFAIGIMKPDGSGERILTSGFHNEGPTFA
PNGRVLMFFRDPGGNAGPSLFTVDVSGRNELRVPTPGYASDPAWSPLLS

Sequences:

>Translated_449_residues
MDCPNMPLHINRRQMLLSAATAAGALALGPARDAFGQARVQITEGNVAPLPIAIPNFVAGTPSDNEVGGGVAQVITNNLK
RSGLFAPIDQAAYVEKITNIDVPPQFKSWTSINAQALVTGRMTRQSDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRI
AHIISDQIYERLTGEKGYFDSRVVFIDESGPADRRVKRLALMDQDGANVRYLTRGSDLVLTPRFSPSTQEITYMEFGQGD
PKVYLFNIETGQREIVGNFPGMSFAPRFSPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRLTDTPAIDTSPSYSPDAARIC
FESDRGGKPQIYVMPASGGQAQRISFGDGSYSTPVWSPRGDYIAFTKQGGGQFAIGIMKPDGSGERILTSGFHNEGPTFA
PNGRVLMFFRDPGGNAGPSLFTVDVSGRNELRVPTPGYASDPAWSPLLS
>Mature_449_residues
MDCPNMPLHINRRQMLLSAATAAGALALGPARDAFGQARVQITEGNVAPLPIAIPNFVAGTPSDNEVGGGVAQVITNNLK
RSGLFAPIDQAAYVEKITNIDVPPQFKSWTSINAQALVTGRMTRQSDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRI
AHIISDQIYERLTGEKGYFDSRVVFIDESGPADRRVKRLALMDQDGANVRYLTRGSDLVLTPRFSPSTQEITYMEFGQGD
PKVYLFNIETGQREIVGNFPGMSFAPRFSPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRLTDTPAIDTSPSYSPDAARIC
FESDRGGKPQIYVMPASGGQAQRISFGDGSYSTPVWSPRGDYIAFTKQGGGQFAIGIMKPDGSGERILTSGFHNEGPTFA
PNGRVLMFFRDPGGNAGPSLFTVDVSGRNELRVPTPGYASDPAWSPLLS

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=422, Percent_Identity=30.3317535545024, Blast_Score=192, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RHOP2 (Q2IZ40)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485431.1
- ProteinModelPortal:   Q2IZ40
- SMR:   Q2IZ40
- STRING:   Q2IZ40
- GeneID:   3908893
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1812
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK05137
- BioCyc:   RPAL316058:RPB_1812-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48897; Mature: 48897

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS51318 TAT; PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCPNMPLHINRRQMLLSAATAAGALALGPARDAFGQARVQITEGNVAPLPIAIPNFVAG
CCCCCCCEEECHHHHHHHHHHHCCEEEECCCHHCCCCEEEEEECCCCCCCCEECCCCCCC
TPSDNEVGGGVAQVITNNLKRSGLFAPIDQAAYVEKITNIDVPPQFKSWTSINAQALVTG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
RMTRQSDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRIAHIISDQIYERLTGEKGYFD
CCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
SRVVFIDESGPADRRVKRLALMDQDGANVRYLTRGSDLVLTPRFSPSTQEITYMEFGQGD
CEEEEEECCCCHHHHHHHHEEECCCCCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCC
PKVYLFNIETGQREIVGNFPGMSFAPRFSPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRL
CEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCEEE
TDTPAIDTSPSYSPDAARICFESDRGGKPQIYVMPASGGQAQRISFGDGSYSTPVWSPRG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCC
DYIAFTKQGGGQFAIGIMKPDGSGERILTSGFHNEGPTFAPNGRVLMFFRDPGGNAGPSL
CEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
FTVDVSGRNELRVPTPGYASDPAWSPLLS
EEEEECCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDCPNMPLHINRRQMLLSAATAAGALALGPARDAFGQARVQITEGNVAPLPIAIPNFVAG
CCCCCCCEEECHHHHHHHHHHHCCEEEECCCHHCCCCEEEEEECCCCCCCCEECCCCCCC
TPSDNEVGGGVAQVITNNLKRSGLFAPIDQAAYVEKITNIDVPPQFKSWTSINAQALVTG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
RMTRQSDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRIAHIISDQIYERLTGEKGYFD
CCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
SRVVFIDESGPADRRVKRLALMDQDGANVRYLTRGSDLVLTPRFSPSTQEITYMEFGQGD
CEEEEEECCCCHHHHHHHHEEECCCCCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCC
PKVYLFNIETGQREIVGNFPGMSFAPRFSPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRL
CEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCEEE
TDTPAIDTSPSYSPDAARICFESDRGGKPQIYVMPASGGQAQRISFGDGSYSTPVWSPRG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCC
DYIAFTKQGGGQFAIGIMKPDGSGERILTSGFHNEGPTFAPNGRVLMFFRDPGGNAGPSL
CEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
FTVDVSGRNELRVPTPGYASDPAWSPLLS
EEEEECCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA