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The map label for this gene is hipO [H]

Identifier: 86748857

GI number: 86748857

Start: 1980211

End: 1981380

Strand: Direct

Name: hipO [H]

Synonym: RPB_1734

Alternate gene names: 86748857

Gene position: 1980211-1981380 (Clockwise)

Preceding gene: 86748855

Following gene: 86748860

Centisome position: 37.14

GC content: 67.69

Gene sequence:

>1170_bases
ATGCCCTTGATCAACCGCGTCGCCGACCTGCAACCCGATATCATGGCCTGGCGTCACGACCTCCATCAGCACCCCGAACT
GATGTACGACGTCGACCGCACCGCCGATTTCGTCGCCCAGCGCCTGCGCGAATTCGGCTGCGACGAGGTGGTGACGGGAC
TCGGCCGCACCGGCGTGGTCGGTGTGATCCGCGGCCGCAAGCCGGCGAGCGGCGACCTCAAGGTGATCGGGCTGCGCGCC
GACATGGACGCGCTGCCGATCGAGGAGGCGACCGGTCTACCCTATGCCTCCAAGGTGCCCGGCAAGATGCACGCCTGCGG
CCATGACGGCCACACCGCGATGCTGCTCGGCGCCGCGCGCTATCTCGCCGAGACCCGCAATTTCGCAGGCAGTGTAGTGG
TGATCTTCCAGCCGGCCGAGGAGGGCGGCGCGGGGGCCGCGGCGATGATCAAGGACGGGCTGATGGACCGCTTCGGCATC
GAGCAGGTCTACGGCATGCACAACGGCCCCGGCATCCCGGTCGGCTCCTTCGCCATCAGCCCGGGCGCGATCATGGCCTC
GACCGATTCGGTCGACATCCGCATCGAGGGCGTCGGCGGCCACGCCGCGCGGCCGCATATGTGCGTCGACTCGGTGCTGG
TGGGCGCCCAGCTCGTCACCGCGCTGCAGTCGATCGTGTCGCGCACGGTCGATCCGCTGGAATCGGCGGTGATCTCGATC
TGCGAATTCCACGCCGGCAACGCCCGCAACGTCATCCCGCAGATCGCCGAACTGAAAGGCACGGTCCGCACCCTGAAGGC
CGAAGTTCGCGACCTGGTCGAGAAGCGCATCCACGAGGTCGCGGCCGGCGTTGCGCAGTCGACCGGCGCCAGGATCGACA
TCGTCTACGAGCGCGGCTACCCGGTGGTGGTCAACCATGCCGAGCAGACCGAGGTGGCGCAGCGGATCGCCCGCGACATC
GCCGGCGAGTCCAACGTGACGTCGATGCCGCCGCTGATGGGCGCCGAGGATTTCGCCTATATGCTGGAAGCGCGGCCGGG
CGCGTTCATCTTCCTCGGCAATGGCGACAGCGCCGGGCTGCATCACCCGGCCTACAACTTCAACGACGACGCCATCGTCT
ACGGCACCTCGTACTGGATCAAACTGGTCGAGAACCAACTCGCGGCGTGA

Upstream 100 bases:

>100_bases
TAGCCCCGCGGCGGGGCGGTGTACAGTTGCCGCCGGGCGGCAGGCCGGCTAGCCTGCCCGATCAGAATACGAAACCAACT
TCCGGAGCCTCGTCAGCCAC

Downstream 100 bases:

>100_bases
CGCCGTCGCCCGGATCGGCTGCGGCCGGTCAGTAGCTCAGCGTGGTGCGGATGCCGAGCACCACCGCGTCCCTGATCCGC
CTGGTCCCGGCGGGATCGAA

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPLINRVADLQPDIMAWRHDLHQHPELMYDVDRTADFVAQRLREFGCDEVVTGLGRTGVVGVIRGRKPASGDLKVIGLRA
DMDALPIEEATGLPYASKVPGKMHACGHDGHTAMLLGAARYLAETRNFAGSVVVIFQPAEEGGAGAAAMIKDGLMDRFGI
EQVYGMHNGPGIPVGSFAISPGAIMASTDSVDIRIEGVGGHAARPHMCVDSVLVGAQLVTALQSIVSRTVDPLESAVISI
CEFHAGNARNVIPQIAELKGTVRTLKAEVRDLVEKRIHEVAAGVAQSTGARIDIVYERGYPVVVNHAEQTEVAQRIARDI
AGESNVTSMPPLMGAEDFAYMLEARPGAFIFLGNGDSAGLHHPAYNFNDDAIVYGTSYWIKLVENQLAA

Sequences:

>Translated_389_residues
MPLINRVADLQPDIMAWRHDLHQHPELMYDVDRTADFVAQRLREFGCDEVVTGLGRTGVVGVIRGRKPASGDLKVIGLRA
DMDALPIEEATGLPYASKVPGKMHACGHDGHTAMLLGAARYLAETRNFAGSVVVIFQPAEEGGAGAAAMIKDGLMDRFGI
EQVYGMHNGPGIPVGSFAISPGAIMASTDSVDIRIEGVGGHAARPHMCVDSVLVGAQLVTALQSIVSRTVDPLESAVISI
CEFHAGNARNVIPQIAELKGTVRTLKAEVRDLVEKRIHEVAAGVAQSTGARIDIVYERGYPVVVNHAEQTEVAQRIARDI
AGESNVTSMPPLMGAEDFAYMLEARPGAFIFLGNGDSAGLHHPAYNFNDDAIVYGTSYWIKLVENQLAA
>Mature_388_residues
PLINRVADLQPDIMAWRHDLHQHPELMYDVDRTADFVAQRLREFGCDEVVTGLGRTGVVGVIRGRKPASGDLKVIGLRAD
MDALPIEEATGLPYASKVPGKMHACGHDGHTAMLLGAARYLAETRNFAGSVVVIFQPAEEGGAGAAAMIKDGLMDRFGIE
QVYGMHNGPGIPVGSFAISPGAIMASTDSVDIRIEGVGGHAARPHMCVDSVLVGAQLVTALQSIVSRTVDPLESAVISIC
EFHAGNARNVIPQIAELKGTVRTLKAEVRDLVEKRIHEVAAGVAQSTGARIDIVYERGYPVVVNHAEQTEVAQRIARDIA
GESNVTSMPPLMGAEDFAYMLEARPGAFIFLGNGDSAGLHHPAYNFNDDAIVYGTSYWIKLVENQLAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=28.1030444964871, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41609; Mature: 41478

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLINRVADLQPDIMAWRHDLHQHPELMYDVDRTADFVAQRLREFGCDEVVTGLGRTGVV
CCHHHHHHHCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRKPASGDLKVIGLRADMDALPIEEATGLPYASKVPGKMHACGHDGHTAMLLGAAR
EEEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
YLAETRNFAGSVVVIFQPAEEGGAGAAAMIKDGLMDRFGIEQVYGMHNGPGIPVGSFAIS
HHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEC
PGAIMASTDSVDIRIEGVGGHAARPHMCVDSVLVGAQLVTALQSIVSRTVDPLESAVISI
CCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CEFHAGNARNVIPQIAELKGTVRTLKAEVRDLVEKRIHEVAAGVAQSTGARIDIVYERGY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PVVVNHAEQTEVAQRIARDIAGESNVTSMPPLMGAEDFAYMLEARPGAFIFLGNGDSAGL
CEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCC
HHPAYNFNDDAIVYGTSYWIKLVENQLAA
CCCCCCCCCCEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLINRVADLQPDIMAWRHDLHQHPELMYDVDRTADFVAQRLREFGCDEVVTGLGRTGVV
CHHHHHHHCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRKPASGDLKVIGLRADMDALPIEEATGLPYASKVPGKMHACGHDGHTAMLLGAAR
EEEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
YLAETRNFAGSVVVIFQPAEEGGAGAAAMIKDGLMDRFGIEQVYGMHNGPGIPVGSFAIS
HHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEC
PGAIMASTDSVDIRIEGVGGHAARPHMCVDSVLVGAQLVTALQSIVSRTVDPLESAVISI
CCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CEFHAGNARNVIPQIAELKGTVRTLKAEVRDLVEKRIHEVAAGVAQSTGARIDIVYERGY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PVVVNHAEQTEVAQRIARDIAGESNVTSMPPLMGAEDFAYMLEARPGAFIFLGNGDSAGL
CEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCC
HHPAYNFNDDAIVYGTSYWIKLVENQLAA
CCCCCCCCCCEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]