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The map label for this gene is mcrB [H]

Identifier: 86748715

GI number: 86748715

Start: 1792089

End: 1794593

Strand: Direct

Name: mcrB [H]

Synonym: RPB_1590

Alternate gene names: 86748715

Gene position: 1792089-1794593 (Clockwise)

Preceding gene: 86748714

Following gene: 86748716

Centisome position: 33.61

GC content: 54.01

Gene sequence:

>2505_bases
ATGAGGGCAGAGGATTTCAAAGCTTGGCTTGAAGCTGGGGGCGCACTTACAGAAGGTGGACGCAACACGCGAGCTAATGC
CGTAGCCACGATCGAGAGAAATCTCGCTGCCTTGGGGTCGCCCAATGCTGATCTCGAAGAAGCATGGAACTCCGATCGAT
TTGAGCATCTGCGTCGCAGACTGAAAGAACTGCGCGAGGAACTCGCGGCGGGCGGGACGCAATATCACGTCCTAATGCCC
CAGTCGGAAAATCCTGAAAAACGACTATCAAGCTGGCGCGCTTGGCTCGGCCAATATGGCCGCTTCTTGAGCGGCGAAAT
ACGTGAGCATCGTGATGCTGATCGGATTCGCCGACACGTACTTGAGAACTACATCGAGCCGGCCAGGGAACGCGATGAAG
AAACGGTGGATGTCCTAGTTCGGAGGGTCAATGACGTACTAGGCCTAAATGAAGCATGGCCTAATATCTGCCAAGCCCTC
CAAGGCAAGAAATTTCGGGAACTGGCCGACTTAGAGGCGCCTGAACAAATCGGTGCGGACCAGAGCCCGGCGACGATCTT
CCGATTTCACTTGGATGAAACTTTCAGGGCTGACGACAATCTCAACTATGCTCGACTGTTTGTCTTATATGATGCCGAGG
GAGCTGCGTTCGAGCCGGTTAAGAACAGAAATCGCGAAACAAATCGCCTAGCGTACCGCATCAAGCCGCGAGGCTCGGGC
AACGAGGCGCGCGATGCGGTCGAAGTCGATACGCTCCAGCAGGTTGCGCGTGCAATGCTCGTTGACCACCTTGCTGCACG
TGTCAAATCCACTCGCGGCGGCGCTGCTAATTATGCCGTCTACGGAAAAGAAAAGATAGTACGTTACGAACTTGATCCGA
GCATTGCATTGGAAATAGGGGTTCCTCCGAATGGCGAGATTGGTCAGAGCAATGATCCCAGCAGCAATCAAGCCCCGCAA
CTTCCAAGCGATAAAGAGGATGGGCTTTTGGAGCAGCCTCACGCAACAAACTTAATTCTCTACGGACCTCCCGGCACAGG
AAAGACCTTCACGACCGCATTAGAGGCGCTCAAGCTTTGCGGTGAACCTGTTCCCGACAGTCGAGAGCACCTAATGGAAG
CCTATCGTCGCCTGTCGGATGCTGGTCGTATCGAATTCGTCACGTTCCATCAATCAATCTCCTACGAAGACTTTGTCGAA
GGGCTGAGGCCAACGCAGTCAGGCGAGGACGGTGCGGCGGGGTTCGGACTGAAGCCCGAGCAAGGAGTATTTCGCAGAAT
AGCGCGCCGCGCTGAGACGAGCACCGGCCCGGGTGATGCCTCCTTCTCGATCAATGGTCGGCAAGTCTTCAAGATGTCGA
TTGGTGAGGCTGCTAACCCAGATGATGCCCATCTGTTCGAGGAGGCCGTCGCCGGTGCCTACACGCTGCTTGGCTTCGAA
GATATTGACTGGAGCGATGATCGTTTCGCTACTCGCGACGCAATCATACAGGCTGTTAGGGCTATCCAAAGCAAGGATCA
GGACGAGCCGAGCGCGATGAGCGGACGCGTTCAGATGCCCTTTATCTTTCGTAACTGGGTCAGGATTGGTGATATTGTCG
TCGTGTCGAAGGGCAACAGCTCATTCCGCGCAATTGGTGAGATCACTGGCGAGTATCAATTTGCTCCGCGTGAAGGCGGC
GACTACGCACACCGCCGTGCGGTTCGGTGGCTTTGGGTGGATCGTACAGGCGTTCCAGTTAGCGAGATCTATGCGCGAAA
TTTCACGCAAAAGTCAATCTACCTCTTGACTGAAGCCGACCTGAATGTCCCAGCGCTGGAGCGCTATATTGCAAGCCAGC
AAGGCACGGGCGGTGGTGCGCCAGAATCGTTTGTTCTTATCATCGACGAAATCAATCGCGCAAATATCTCAAAAGTGTTC
GGCGAACTAATTACATTGCTCGAACCGGATAAGCGCATTGGTCGGCCCAATGCTCTTAAAGTGCGGCTTCCCTATTCTGG
GGATTTGTTCGGCGTTCCGGCAAACCTTCACATCATCGGTACGATGAACACAGCAGATCGCTCGATCGCATTGCTTGACA
CAGCTCTCCGGCGCCGTTTTGAATTTCGGGAACTAATGCCCGATCCTTCGCTGCTCGGTATCGTGGACGGTGTTGATCTT
TCCCGACTGCTCGCTACGATCAATCAACGAATTGAATATTTGTTCGATCGGGAGCATCAGATAGGCCACGCCTATTTTAT
CAGCTGCAAATCTCGTGCCGATATAGATGAGGTGATGCGTTGCAAAGTGATTCCATTGCTGGCCGAATATTTTTATGAGG
ATTGGTCGAAGGTTGCCGCCGCGTTGGGTGACGGTGACGACAGCGAGGTCGACCTCGAAGGACATTTCATTGATCGCCAA
CGTTTGAAGCCACCCAAGGGCATAAGCAGCGATGGAGATGGAGCGGTGCGCTATCGCTGGACCATCCGACGTGCCTTTTC
ATATAGCGAGTTCTCGGCAGGATGA

Upstream 100 bases:

>100_bases
GGGGATTCCCCGGCCATTTTTTTTGCTAGCATTTCTCTATTTTCAAGTGTGATTGCGTCTGGGTGCAAATTCAGTCCGTA
TTTCGCAAGACGGGGGCGAT

Downstream 100 bases:

>100_bases
TTCGTCGAACCATCCTTGAGTGGGAGACGATACGTTACGGTGATGCGGCTGATGAAATCCCTGCCGATGCCGCTGATCGG
ATTGCAGCGGTGGCGTCGGC

Product: AAA_5 ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 834; Mature: 834

Protein sequence:

>834_residues
MRAEDFKAWLEAGGALTEGGRNTRANAVATIERNLAALGSPNADLEEAWNSDRFEHLRRRLKELREELAAGGTQYHVLMP
QSENPEKRLSSWRAWLGQYGRFLSGEIREHRDADRIRRHVLENYIEPARERDEETVDVLVRRVNDVLGLNEAWPNICQAL
QGKKFRELADLEAPEQIGADQSPATIFRFHLDETFRADDNLNYARLFVLYDAEGAAFEPVKNRNRETNRLAYRIKPRGSG
NEARDAVEVDTLQQVARAMLVDHLAARVKSTRGGAANYAVYGKEKIVRYELDPSIALEIGVPPNGEIGQSNDPSSNQAPQ
LPSDKEDGLLEQPHATNLILYGPPGTGKTFTTALEALKLCGEPVPDSREHLMEAYRRLSDAGRIEFVTFHQSISYEDFVE
GLRPTQSGEDGAAGFGLKPEQGVFRRIARRAETSTGPGDASFSINGRQVFKMSIGEAANPDDAHLFEEAVAGAYTLLGFE
DIDWSDDRFATRDAIIQAVRAIQSKDQDEPSAMSGRVQMPFIFRNWVRIGDIVVVSKGNSSFRAIGEITGEYQFAPREGG
DYAHRRAVRWLWVDRTGVPVSEIYARNFTQKSIYLLTEADLNVPALERYIASQQGTGGGAPESFVLIIDEINRANISKVF
GELITLLEPDKRIGRPNALKVRLPYSGDLFGVPANLHIIGTMNTADRSIALLDTALRRRFEFRELMPDPSLLGIVDGVDL
SRLLATINQRIEYLFDREHQIGHAYFISCKSRADIDEVMRCKVIPLLAEYFYEDWSKVAAALGDGDDSEVDLEGHFIDRQ
RLKPPKGISSDGDGAVRYRWTIRRAFSYSEFSAG

Sequences:

>Translated_834_residues
MRAEDFKAWLEAGGALTEGGRNTRANAVATIERNLAALGSPNADLEEAWNSDRFEHLRRRLKELREELAAGGTQYHVLMP
QSENPEKRLSSWRAWLGQYGRFLSGEIREHRDADRIRRHVLENYIEPARERDEETVDVLVRRVNDVLGLNEAWPNICQAL
QGKKFRELADLEAPEQIGADQSPATIFRFHLDETFRADDNLNYARLFVLYDAEGAAFEPVKNRNRETNRLAYRIKPRGSG
NEARDAVEVDTLQQVARAMLVDHLAARVKSTRGGAANYAVYGKEKIVRYELDPSIALEIGVPPNGEIGQSNDPSSNQAPQ
LPSDKEDGLLEQPHATNLILYGPPGTGKTFTTALEALKLCGEPVPDSREHLMEAYRRLSDAGRIEFVTFHQSISYEDFVE
GLRPTQSGEDGAAGFGLKPEQGVFRRIARRAETSTGPGDASFSINGRQVFKMSIGEAANPDDAHLFEEAVAGAYTLLGFE
DIDWSDDRFATRDAIIQAVRAIQSKDQDEPSAMSGRVQMPFIFRNWVRIGDIVVVSKGNSSFRAIGEITGEYQFAPREGG
DYAHRRAVRWLWVDRTGVPVSEIYARNFTQKSIYLLTEADLNVPALERYIASQQGTGGGAPESFVLIIDEINRANISKVF
GELITLLEPDKRIGRPNALKVRLPYSGDLFGVPANLHIIGTMNTADRSIALLDTALRRRFEFRELMPDPSLLGIVDGVDL
SRLLATINQRIEYLFDREHQIGHAYFISCKSRADIDEVMRCKVIPLLAEYFYEDWSKVAAALGDGDDSEVDLEGHFIDRQ
RLKPPKGISSDGDGAVRYRWTIRRAFSYSEFSAG
>Mature_834_residues
MRAEDFKAWLEAGGALTEGGRNTRANAVATIERNLAALGSPNADLEEAWNSDRFEHLRRRLKELREELAAGGTQYHVLMP
QSENPEKRLSSWRAWLGQYGRFLSGEIREHRDADRIRRHVLENYIEPARERDEETVDVLVRRVNDVLGLNEAWPNICQAL
QGKKFRELADLEAPEQIGADQSPATIFRFHLDETFRADDNLNYARLFVLYDAEGAAFEPVKNRNRETNRLAYRIKPRGSG
NEARDAVEVDTLQQVARAMLVDHLAARVKSTRGGAANYAVYGKEKIVRYELDPSIALEIGVPPNGEIGQSNDPSSNQAPQ
LPSDKEDGLLEQPHATNLILYGPPGTGKTFTTALEALKLCGEPVPDSREHLMEAYRRLSDAGRIEFVTFHQSISYEDFVE
GLRPTQSGEDGAAGFGLKPEQGVFRRIARRAETSTGPGDASFSINGRQVFKMSIGEAANPDDAHLFEEAVAGAYTLLGFE
DIDWSDDRFATRDAIIQAVRAIQSKDQDEPSAMSGRVQMPFIFRNWVRIGDIVVVSKGNSSFRAIGEITGEYQFAPREGG
DYAHRRAVRWLWVDRTGVPVSEIYARNFTQKSIYLLTEADLNVPALERYIASQQGTGGGAPESFVLIIDEINRANISKVF
GELITLLEPDKRIGRPNALKVRLPYSGDLFGVPANLHIIGTMNTADRSIALLDTALRRRFEFRELMPDPSLLGIVDGVDL
SRLLATINQRIEYLFDREHQIGHAYFISCKSRADIDEVMRCKVIPLLAEYFYEDWSKVAAALGDGDDSEVDLEGHFIDRQ
RLKPPKGISSDGDGAVRYRWTIRRAFSYSEFSAG

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=183, Percent_Identity=37.7049180327869, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 93397; Mature: 93397

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEDFKAWLEAGGALTEGGRNTRANAVATIERNLAALGSPNADLEEAWNSDRFEHLRRR
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHH
LKELREELAAGGTQYHVLMPQSENPEKRLSSWRAWLGQYGRFLSGEIREHRDADRIRRHV
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
LENYIEPARERDEETVDVLVRRVNDVLGLNEAWPNICQALQGKKFRELADLEAPEQIGAD
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCHHHCCCC
QSPATIFRFHLDETFRADDNLNYARLFVLYDAEGAAFEPVKNRNRETNRLAYRIKPRGSG
CCCCEEEEEECCCHHCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCEEEEEEECCCCC
NEARDAVEVDTLQQVARAMLVDHLAARVKSTRGGAANYAVYGKEKIVRYELDPSIALEIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEEEEC
VPPNGEIGQSNDPSSNQAPQLPSDKEDGLLEQPHATNLILYGPPGTGKTFTTALEALKLC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GEPVPDSREHLMEAYRRLSDAGRIEFVTFHQSISYEDFVEGLRPTQSGEDGAAGFGLKPE
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
QGVFRRIARRAETSTGPGDASFSINGRQVFKMSIGEAANPDDAHLFEEAVAGAYTLLGFE
HHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECC
DIDWSDDRFATRDAIIQAVRAIQSKDQDEPSAMSGRVQMPFIFRNWVRIGDIVVVSKGNS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCHHHHHHHEECCEEEEECCCC
SFRAIGEITGEYQFAPREGGDYAHRRAVRWLWVDRTGVPVSEIYARNFTQKSIYLLTEAD
CHHHHHCCCCCEEECCCCCCCHHHHHEEEEEEEECCCCCHHHHHHCCCCCCEEEEEEECC
LNVPALERYIASQQGTGGGAPESFVLIIDEINRANISKVFGELITLLEPDKRIGRPNALK
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCEEE
VRLPYSGDLFGVPANLHIIGTMNTADRSIALLDTALRRRFEFRELMPDPSLLGIVDGVDL
EEECCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCH
SRLLATINQRIEYLFDREHQIGHAYFISCKSRADIDEVMRCKVIPLLAEYFYEDWSKVAA
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGDGDDSEVDLEGHFIDRQRLKPPKGISSDGDGAVRYRWTIRRAFSYSEFSAG
HHCCCCCCEEEECCEEECHHHCCCCCCCCCCCCCEEEEEEEEEHHHCCHHCCCC
>Mature Secondary Structure
MRAEDFKAWLEAGGALTEGGRNTRANAVATIERNLAALGSPNADLEEAWNSDRFEHLRRR
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHH
LKELREELAAGGTQYHVLMPQSENPEKRLSSWRAWLGQYGRFLSGEIREHRDADRIRRHV
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
LENYIEPARERDEETVDVLVRRVNDVLGLNEAWPNICQALQGKKFRELADLEAPEQIGAD
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCHHHCCCC
QSPATIFRFHLDETFRADDNLNYARLFVLYDAEGAAFEPVKNRNRETNRLAYRIKPRGSG
CCCCEEEEEECCCHHCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCEEEEEEECCCCC
NEARDAVEVDTLQQVARAMLVDHLAARVKSTRGGAANYAVYGKEKIVRYELDPSIALEIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEEEEC
VPPNGEIGQSNDPSSNQAPQLPSDKEDGLLEQPHATNLILYGPPGTGKTFTTALEALKLC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GEPVPDSREHLMEAYRRLSDAGRIEFVTFHQSISYEDFVEGLRPTQSGEDGAAGFGLKPE
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
QGVFRRIARRAETSTGPGDASFSINGRQVFKMSIGEAANPDDAHLFEEAVAGAYTLLGFE
HHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECC
DIDWSDDRFATRDAIIQAVRAIQSKDQDEPSAMSGRVQMPFIFRNWVRIGDIVVVSKGNS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCHHHHHHHEECCEEEEECCCC
SFRAIGEITGEYQFAPREGGDYAHRRAVRWLWVDRTGVPVSEIYARNFTQKSIYLLTEAD
CHHHHHCCCCCEEECCCCCCCHHHHHEEEEEEEECCCCCHHHHHHCCCCCCEEEEEEECC
LNVPALERYIASQQGTGGGAPESFVLIIDEINRANISKVFGELITLLEPDKRIGRPNALK
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCEEE
VRLPYSGDLFGVPANLHIIGTMNTADRSIALLDTALRRRFEFRELMPDPSLLGIVDGVDL
EEECCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCH
SRLLATINQRIEYLFDREHQIGHAYFISCKSRADIDEVMRCKVIPLLAEYFYEDWSKVAA
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGDGDDSEVDLEGHFIDRQRLKPPKGISSDGDGAVRYRWTIRRAFSYSEFSAG
HHCCCCCCEEEECCEEECHHHCCCCCCCCCCCCCEEEEEEEEEHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]