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The map label for this gene is braE [H]

Identifier: 86748697

GI number: 86748697

Start: 1769660

End: 1770961

Strand: Reverse

Name: braE [H]

Synonym: RPB_1572

Alternate gene names: 86748697

Gene position: 1770961-1769660 (Counterclockwise)

Preceding gene: 86748698

Following gene: 86748696

Centisome position: 33.22

GC content: 66.28

Gene sequence:

>1302_bases
ATGACCGACCTCGCTTCCCGCGTCGCGCCGCCGCGACCGGCGCTGTCCGATCGCCTCAAATTCTACGGGGTCTGGATCGC
CACAGCGCTGACGCTGCTGCTGCTGCCGCAGATCTTCTCATCCGGCGGATCGCTGACGACCTTCAGCCTGGTCGGCATCT
CGATCATCTTCGCCTTGTCCTACAACATCCTGCTCGGCCAGACCGGCATGCTGTCGTTCGGCCACGCGGTGTATTACGGC
CTCGGCGGCTTCCTGGCGATCCACGCCATCAATCTGCTCGGCGCCAACAAATGGGCGATCCCGCTGCCGCTGGTGCCGCT
GATCGGCGGCATGGCGGGGCTGTTCCTCGCGGCCCTGATCGGCTGGGTGATGACGCAGCGCTCGGGCACCGCGTTCGCGA
TGATCTCGCTCGGCCTCGCCGAACTGGTCGCCTCCTCGGCGCTGATCCTGCGCACCTTCTTCGGCGGCGAGGCCGGCATC
TCGGCCAACCGCACCAAGCTGTTCAAGGTGTTCGGGCTGTCCTTCGGGCCGCAGATCCAGATCTATTATCTGGTCGCCGC
CTGGACGCTGATCGCGATCATCGCGATGTACGCGCTGACCCGGACGCCGCTCGGCCGGATGTGCAACGCGGTCCGCGACA
ATCCTGAGCGCGTGCAGTTCGTCGGCTACGACCCACACGTCGTGCGCTATCTGGCGTTCTGCTTTGCGGGGTTCTTCGCC
GGCATCGCCGGCGCGCTCGCCGCGATCAATTTCGAGATCGCCAACTCGGCCTATCTCGGCGCGGTGCAGTCCGGCACCGT
GCTGTTCGCGGCCTATATCGGCGGCATCGGCTTCTTCATCGGCCCGATCGTCGGGGCGATCTTCGTCACCATCCTGTCGC
TAGGCCTGTCCGACCTCACCCAGGTCTGGCAATTGTATTTCGGCCTGTTCTTCATCGCCGTGGTGCTGTACGCGCCCGGC
GGCATCACAGGCCTGTTGATGATGCACCGGCCGCTGATCCGTGCCGGCATGCTGTCGCGGATGCTGCCGAGCTATCTCAT
CGCCGCGCTGCCGACGCTGGCGCTGGTCACCGGGCTGATGCTGATGATCGAGACCATCGTGCACTACACCGTCAATCCCC
ACGAGGATCCCTACCGCAAGGCGTTCGGCATCCCGTTCGACGCGGCGAGCCCGCTGACCTGGGCGATCTCCGCGGCGCTG
ATCGTGGGCGGCTTCTGGCTGTGCCGGATGGCCTGGGCCCGCGTCGGCCAGACCTGGGACGAGACCCTCACCGCCGCCCG
CGCCAAGGTGCATGCCCTATGA

Upstream 100 bases:

>100_bases
GGAACGTGACGATCGCGCAGATCGCGCCGATCATGCCGTATCTGCTGCTCGTGCTGATCCTGATCTTCCGGCCGATGGGG
CTGTTGGGAACCCGCGAAAC

Downstream 100 bases:

>100_bases
GCAACGCCATCGAACTGCACGGCGTCGAGAAGAGCTTCGGCATCACCAAGGTGATCCAGAACATCACGCTCAGCATCGCC
CAGGGCGAACGCCACGCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTDLASRVAPPRPALSDRLKFYGVWIATALTLLLLPQIFSSGGSLTTFSLVGISIIFALSYNILLGQTGMLSFGHAVYYG
LGGFLAIHAINLLGANKWAIPLPLVPLIGGMAGLFLAALIGWVMTQRSGTAFAMISLGLAELVASSALILRTFFGGEAGI
SANRTKLFKVFGLSFGPQIQIYYLVAAWTLIAIIAMYALTRTPLGRMCNAVRDNPERVQFVGYDPHVVRYLAFCFAGFFA
GIAGALAAINFEIANSAYLGAVQSGTVLFAAYIGGIGFFIGPIVGAIFVTILSLGLSDLTQVWQLYFGLFFIAVVLYAPG
GITGLLMMHRPLIRAGMLSRMLPSYLIAALPTLALVTGLMLMIETIVHYTVNPHEDPYRKAFGIPFDAASPLTWAISAAL
IVGGFWLCRMAWARVGQTWDETLTAARAKVHAL

Sequences:

>Translated_433_residues
MTDLASRVAPPRPALSDRLKFYGVWIATALTLLLLPQIFSSGGSLTTFSLVGISIIFALSYNILLGQTGMLSFGHAVYYG
LGGFLAIHAINLLGANKWAIPLPLVPLIGGMAGLFLAALIGWVMTQRSGTAFAMISLGLAELVASSALILRTFFGGEAGI
SANRTKLFKVFGLSFGPQIQIYYLVAAWTLIAIIAMYALTRTPLGRMCNAVRDNPERVQFVGYDPHVVRYLAFCFAGFFA
GIAGALAAINFEIANSAYLGAVQSGTVLFAAYIGGIGFFIGPIVGAIFVTILSLGLSDLTQVWQLYFGLFFIAVVLYAPG
GITGLLMMHRPLIRAGMLSRMLPSYLIAALPTLALVTGLMLMIETIVHYTVNPHEDPYRKAFGIPFDAASPLTWAISAAL
IVGGFWLCRMAWARVGQTWDETLTAARAKVHAL
>Mature_432_residues
TDLASRVAPPRPALSDRLKFYGVWIATALTLLLLPQIFSSGGSLTTFSLVGISIIFALSYNILLGQTGMLSFGHAVYYGL
GGFLAIHAINLLGANKWAIPLPLVPLIGGMAGLFLAALIGWVMTQRSGTAFAMISLGLAELVASSALILRTFFGGEAGIS
ANRTKLFKVFGLSFGPQIQIYYLVAAWTLIAIIAMYALTRTPLGRMCNAVRDNPERVQFVGYDPHVVRYLAFCFAGFFAG
IAGALAAINFEIANSAYLGAVQSGTVLFAAYIGGIGFFIGPIVGAIFVTILSLGLSDLTQVWQLYFGLFFIAVVLYAPGG
ITGLLMMHRPLIRAGMLSRMLPSYLIAALPTLALVTGLMLMIETIVHYTVNPHEDPYRKAFGIPFDAASPLTWAISAALI
VGGFWLCRMAWARVGQTWDETLTAARAKVHAL

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=315, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46435; Mature: 46304

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLASRVAPPRPALSDRLKFYGVWIATALTLLLLPQIFSSGGSLTTFSLVGISIIFALS
CCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YNILLGQTGMLSFGHAVYYGLGGFLAIHAINLLGANKWAIPLPLVPLIGGMAGLFLAALI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GWVMTQRSGTAFAMISLGLAELVASSALILRTFFGGEAGISANRTKLFKVFGLSFGPQIQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEE
IYYLVAAWTLIAIIAMYALTRTPLGRMCNAVRDNPERVQFVGYDPHVVRYLAFCFAGFFA
EHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHH
GIAGALAAINFEIANSAYLGAVQSGTVLFAAYIGGIGFFIGPIVGAIFVTILSLGLSDLT
HHHHHHHHHHHEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
QVWQLYFGLFFIAVVLYAPGGITGLLMMHRPLIRAGMLSRMLPSYLIAALPTLALVTGLM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMIETIVHYTVNPHEDPYRKAFGIPFDAASPLTWAISAALIVGGFWLCRMAWARVGQTWD
HHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETLTAARAKVHAL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TDLASRVAPPRPALSDRLKFYGVWIATALTLLLLPQIFSSGGSLTTFSLVGISIIFALS
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YNILLGQTGMLSFGHAVYYGLGGFLAIHAINLLGANKWAIPLPLVPLIGGMAGLFLAALI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GWVMTQRSGTAFAMISLGLAELVASSALILRTFFGGEAGISANRTKLFKVFGLSFGPQIQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEE
IYYLVAAWTLIAIIAMYALTRTPLGRMCNAVRDNPERVQFVGYDPHVVRYLAFCFAGFFA
EHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHH
GIAGALAAINFEIANSAYLGAVQSGTVLFAAYIGGIGFFIGPIVGAIFVTILSLGLSDLT
HHHHHHHHHHHEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
QVWQLYFGLFFIAVVLYAPGGITGLLMMHRPLIRAGMLSRMLPSYLIAALPTLALVTGLM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMIETIVHYTVNPHEDPYRKAFGIPFDAASPLTWAISAALIVGGFWLCRMAWARVGQTWD
HHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETLTAARAKVHAL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]