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The map label for this gene is rffG [H]

Identifier: 86748687

GI number: 86748687

Start: 1761212

End: 1762273

Strand: Direct

Name: rffG [H]

Synonym: RPB_1562

Alternate gene names: 86748687

Gene position: 1761212-1762273 (Clockwise)

Preceding gene: 86748684

Following gene: 86748688

Centisome position: 33.03

GC content: 65.35

Gene sequence:

>1062_bases
ATGACCAGACGATTCATCATCACAGGCGGCGCGGGCTTCATCGGCTCGGCGGTGGTCCGTCGTCTGATCGAGACGACCGG
CCACGAGGTGCTCGTCGTCGACAAGCTGACCTATGCGGGCAATCTGGAATCGCTCGCCCCGGTGTCGTCCGATCCGCGCT
TTCGTTTCGAACGCGCCGACATCGCCGATGCCGGCGCGATCCGGCGGATCGTCGCCGACTTCTCCCCGGATGTGGTGATG
CACCTCGCCGCGGAAAGTCATGTCGACCGCTCGATCGACGGTCCGGCCGAGTTCATTCAGACCAACGTCGTCGGCACCTT
CGTTCTGCTGCAGGCGGCGCTGGCGCATTGGCGCGCGCTGCCGGCCGATCGGAAGGCCGGTTTCCGCTTCCACCACATCT
CGACCGACGAAGTGTTCGGCTCACTCGGGCCGACCGGCTATTTCGACGAGCAGACGGCCTACCAGCCCAATTCACCCTAT
TCGGCATCGAAGGCCGGGTCCGACCATCTGGTGCGCGCCTGGCATCACACCTACGGCCTGCCGACGCTGATGACCAATTG
CTCGAACAATTACGGCCCGTATCAGTTTCCGGAAAAGCTGATCGCGCTGACGATCATCAATGCGCTGGAAGGCAAGCCGC
TCCCGGTCTACGGCGCCGGCACCAATGTGCGCGACTGGCTGCATGTCGACGATCACGCCGAAGCGCTGCTGCTGGTCGCC
GCCGAGGGCGCCGTCGGCGACAGCTACAACATCGGCGGGCACAACGAGCACACCAATCTTTCGGTGGTGCGCGACATCTG
CCGCATCGTCGACGAACTCGCGCCGGACAGCGCCATCGGTCCGCGCGAGCAACTGATCGCATTCGTCGTCGACCGGCCGG
GCCACGACCTGCGCTACGCCATCGATGCGAGCAAGATCGGTCGCGACCTCGGCTGGCGGCCGCGCTACGATTTCGAGACG
GGGTTGCGTCGGACCGTGCAATGGTATGTCGCCAATGTCGACTGGTGGAAGCGGGTCCGCTCCGGCGCGTACCGGGGCGA
ACGCCTCGGGCTCGCGGTCTGA

Upstream 100 bases:

>100_bases
GCAATCGTCGCCGATCGGCGTGCCGGGGCGCGGCCGCCGCCGGCTGCATCGGGCGTCCGTCGCCCCGAGCAACGATGCCC
GTCCGGAAACAGGCGAACCG

Downstream 100 bases:

>100_bases
CGACGGCCCGGGAGAACATTCGTGCTGACAGTGACGCCGACCGCGATCGAAGCCGTCAAGATCATCGTTCCCAAGGGCTT
CGCCGATTCGCGCGGGGTGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTRRFIITGGAGFIGSAVVRRLIETTGHEVLVVDKLTYAGNLESLAPVSSDPRFRFERADIADAGAIRRIVADFSPDVVM
HLAAESHVDRSIDGPAEFIQTNVVGTFVLLQAALAHWRALPADRKAGFRFHHISTDEVFGSLGPTGYFDEQTAYQPNSPY
SASKAGSDHLVRAWHHTYGLPTLMTNCSNNYGPYQFPEKLIALTIINALEGKPLPVYGAGTNVRDWLHVDDHAEALLLVA
AEGAVGDSYNIGGHNEHTNLSVVRDICRIVDELAPDSAIGPREQLIAFVVDRPGHDLRYAIDASKIGRDLGWRPRYDFET
GLRRTVQWYVANVDWWKRVRSGAYRGERLGLAV

Sequences:

>Translated_353_residues
MTRRFIITGGAGFIGSAVVRRLIETTGHEVLVVDKLTYAGNLESLAPVSSDPRFRFERADIADAGAIRRIVADFSPDVVM
HLAAESHVDRSIDGPAEFIQTNVVGTFVLLQAALAHWRALPADRKAGFRFHHISTDEVFGSLGPTGYFDEQTAYQPNSPY
SASKAGSDHLVRAWHHTYGLPTLMTNCSNNYGPYQFPEKLIALTIINALEGKPLPVYGAGTNVRDWLHVDDHAEALLLVA
AEGAVGDSYNIGGHNEHTNLSVVRDICRIVDELAPDSAIGPREQLIAFVVDRPGHDLRYAIDASKIGRDLGWRPRYDFET
GLRRTVQWYVANVDWWKRVRSGAYRGERLGLAV
>Mature_352_residues
TRRFIITGGAGFIGSAVVRRLIETTGHEVLVVDKLTYAGNLESLAPVSSDPRFRFERADIADAGAIRRIVADFSPDVVMH
LAAESHVDRSIDGPAEFIQTNVVGTFVLLQAALAHWRALPADRKAGFRFHHISTDEVFGSLGPTGYFDEQTAYQPNSPYS
ASKAGSDHLVRAWHHTYGLPTLMTNCSNNYGPYQFPEKLIALTIINALEGKPLPVYGAGTNVRDWLHVDDHAEALLLVAA
EGAVGDSYNIGGHNEHTNLSVVRDICRIVDELAPDSAIGPREQLIAFVVDRPGHDLRYAIDASKIGRDLGWRPRYDFETG
LRRTVQWYVANVDWWKRVRSGAYRGERLGLAV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=33.7209302325581, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=25.3687315634218, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56237023, Length=325, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI56118217, Length=325, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI189083684, Length=325, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=23.0320699708455, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=65.3409090909091, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=62.2159090909091, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=26.1707988980716, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=26.4957264957265, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=35.5623100303951, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=31.1178247734139, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=24.5614035087719, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI133901786, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=333, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=24.9249249249249, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24158427, Length=334, Percent_Identity=24.5508982035928, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=26.5927977839335, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39015; Mature: 38884

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRFIITGGAGFIGSAVVRRLIETTGHEVLVVDKLTYAGNLESLAPVSSDPRFRFERAD
CCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHCCCCCCCCCEEEECCC
IADAGAIRRIVADFSPDVVMHLAAESHVDRSIDGPAEFIQTNVVGTFVLLQAALAHWRAL
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PADRKAGFRFHHISTDEVFGSLGPTGYFDEQTAYQPNSPYSASKAGSDHLVRAWHHTYGL
CCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLMTNCSNNYGPYQFPEKLIALTIINALEGKPLPVYGAGTNVRDWLHVDDHAEALLLVA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEEEEEE
AEGAVGDSYNIGGHNEHTNLSVVRDICRIVDELAPDSAIGPREQLIAFVVDRPGHDLRYA
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCEEEE
IDASKIGRDLGWRPRYDFETGLRRTVQWYVANVDWWKRVRSGAYRGERLGLAV
ECHHHHHHCCCCCCCCCHHHHHHHEEHHHEECCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TRRFIITGGAGFIGSAVVRRLIETTGHEVLVVDKLTYAGNLESLAPVSSDPRFRFERAD
CCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHCCCCCCCCCEEEECCC
IADAGAIRRIVADFSPDVVMHLAAESHVDRSIDGPAEFIQTNVVGTFVLLQAALAHWRAL
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PADRKAGFRFHHISTDEVFGSLGPTGYFDEQTAYQPNSPYSASKAGSDHLVRAWHHTYGL
CCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLMTNCSNNYGPYQFPEKLIALTIINALEGKPLPVYGAGTNVRDWLHVDDHAEALLLVA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEEEEEE
AEGAVGDSYNIGGHNEHTNLSVVRDICRIVDELAPDSAIGPREQLIAFVVDRPGHDLRYA
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCEEEE
IDASKIGRDLGWRPRYDFETGLRRTVQWYVANVDWWKRVRSGAYRGERLGLAV
ECHHHHHHCCCCCCCCCHHHHHHHEEHHHEECCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]