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The map label for this gene is fcl [C]

Identifier: 86748653

GI number: 86748653

Start: 1721267

End: 1722280

Strand: Direct

Name: fcl [C]

Synonym: RPB_1528

Alternate gene names: 86748653

Gene position: 1721267-1722280 (Clockwise)

Preceding gene: 86748652

Following gene: 86748655

Centisome position: 32.28

GC content: 63.21

Gene sequence:

>1014_bases
ATGGCAGGTTTAGCCTTTGATCTGACGGGCCGCCGCGTCTGGGTGGCGGGCCATCGTGGCATGCTGGGATCCGCGTTGGT
GCGACGGCTTTCGCGTGAGAATTGCGAGATTCTGACCGTCGGACGTGATGAACTCGATCTGCGCCACCAGACCAAGGTGC
AGGAGTGGTTCAGCTCCGAACGCCCGGACGTGGTGATCCTCGCCGCAGCTCGGGTCGGCGGCGTCCTCGCGAACAGCAAA
TATCCGGCCAGCTTTCTCTCCGACAATCTCTCGATACAGGACAACGTCATTCAGTCCGCCGCCGCGGCCGGAGTGAAGAA
ACTGCTGTTCGTCAGTTCGTCCTGCGTCTACCCGCGACTGGCGAGCCAGCCGATCGAAGAAGATGCGTTGCTGACCGGAG
CCCTGGAGCCCACCAATCGGTGGTACGGCGTCGCCAAGATCGCCGGCATGATGCAATGCGCGGCCTACCGCGAGCAATAC
GGCTGTGATTTCATCGCGGCCGTGCCTGGAAACCTCTACGGGCCGGGCGATTACTTCGACAAGGAGAATTCCCACGTCAT
CCCCGCGTTTCTCCGGCGCTTTCACGATGCGGTCACCACGGGAGCCGACGAGGTCGCGGTCTGGGGCAGCGGCACCGCGC
GGCGAGAGTTCGTCTTCGTCGACGAGTGCGCCGACGCGTTGGTGTTCCTTCTGCGCTCCTATTCGTCCGGCGAGATCGTC
AACATCGGAAGCGGAGTCGATGTCTCGATCAGCGAACTCGCGCACCTCGTCGCGGATGTGACCGGCTTTCGGGGGCGCAT
CGCGTTCGATACGTCGCAACCGGAAGGAGCGCCCCGGCGCCTGCTCAGCACGAAGCGGCTCGGTGAGCTGGGTTGGCAAT
CGCAGATGCAATTGCGGGAAGGCCTGAGCAGGACCTATCAGTGGTTTCTGGACAATCATTCGGCGGGAACGGTGCTGCGC
GGCTACGAGACGGCGGACACCAGCCGAGACGTCGATACGATCAGGACGCGCTGA

Upstream 100 bases:

>100_bases
GTCAACTTGTGTCCCATGTCGTTGATGCCTATAAGATCGATCTTGCGAGCGCCCGCGGCCGGTCGAAACCGTCGAAGAGG
CGCAATGGAGTTGATCCTCA

Downstream 100 bases:

>100_bases
CGGATTGCGGTTCGCCGATGGTCGCTGTCGTCATCGCGGCGCGACGAGGTGGGACGTCACTCTCGCCATGAAATCGCCGG
CGGCGCGGTCGGGGTGAAAA

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAGLAFDLTGRRVWVAGHRGMLGSALVRRLSRENCEILTVGRDELDLRHQTKVQEWFSSERPDVVILAAARVGGVLANSK
YPASFLSDNLSIQDNVIQSAAAAGVKKLLFVSSSCVYPRLASQPIEEDALLTGALEPTNRWYGVAKIAGMMQCAAYREQY
GCDFIAAVPGNLYGPGDYFDKENSHVIPAFLRRFHDAVTTGADEVAVWGSGTARREFVFVDECADALVFLLRSYSSGEIV
NIGSGVDVSISELAHLVADVTGFRGRIAFDTSQPEGAPRRLLSTKRLGELGWQSQMQLREGLSRTYQWFLDNHSAGTVLR
GYETADTSRDVDTIRTR

Sequences:

>Translated_337_residues
MAGLAFDLTGRRVWVAGHRGMLGSALVRRLSRENCEILTVGRDELDLRHQTKVQEWFSSERPDVVILAAARVGGVLANSK
YPASFLSDNLSIQDNVIQSAAAAGVKKLLFVSSSCVYPRLASQPIEEDALLTGALEPTNRWYGVAKIAGMMQCAAYREQY
GCDFIAAVPGNLYGPGDYFDKENSHVIPAFLRRFHDAVTTGADEVAVWGSGTARREFVFVDECADALVFLLRSYSSGEIV
NIGSGVDVSISELAHLVADVTGFRGRIAFDTSQPEGAPRRLLSTKRLGELGWQSQMQLREGLSRTYQWFLDNHSAGTVLR
GYETADTSRDVDTIRTR
>Mature_336_residues
AGLAFDLTGRRVWVAGHRGMLGSALVRRLSRENCEILTVGRDELDLRHQTKVQEWFSSERPDVVILAAARVGGVLANSKY
PASFLSDNLSIQDNVIQSAAAAGVKKLLFVSSSCVYPRLASQPIEEDALLTGALEPTNRWYGVAKIAGMMQCAAYREQYG
CDFIAAVPGNLYGPGDYFDKENSHVIPAFLRRFHDAVTTGADEVAVWGSGTARREFVFVDECADALVFLLRSYSSGEIVN
IGSGVDVSISELAHLVADVTGFRGRIAFDTSQPEGAPRRLLSTKRLGELGWQSQMQLREGLSRTYQWFLDNHSAGTVLRG
YETADTSRDVDTIRTR

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=309, Percent_Identity=31.0679611650485, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=23.1884057971014, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=25.3968253968254, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788365, Length=315, Percent_Identity=49.5238095238095, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=23.8505747126437, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=24.7838616714697, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=313, Percent_Identity=27.7955271565495, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17568069, Length=253, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115532424, Length=277, Percent_Identity=22.0216606498195, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922778, Length=315, Percent_Identity=32.6984126984127, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 37004; Mature: 36873

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLAFDLTGRRVWVAGHRGMLGSALVRRLSRENCEILTVGRDELDLRHQTKVQEWFSSE
CCCEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCC
RPDVVILAAARVGGVLANSKYPASFLSDNLSIQDNVIQSAAAAGVKKLLFVSSSCVYPRL
CCCEEEEEEHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ASQPIEEDALLTGALEPTNRWYGVAKIAGMMQCAAYREQYGCDFIAAVPGNLYGPGDYFD
HCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
KENSHVIPAFLRRFHDAVTTGADEVAVWGSGTARREFVFVDECADALVFLLRSYSSGEIV
CCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEE
NIGSGVDVSISELAHLVADVTGFRGRIAFDTSQPEGAPRRLLSTKRLGELGWQSQMQLRE
EECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GLSRTYQWFLDNHSAGTVLRGYETADTSRDVDTIRTR
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
AGLAFDLTGRRVWVAGHRGMLGSALVRRLSRENCEILTVGRDELDLRHQTKVQEWFSSE
CCEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCC
RPDVVILAAARVGGVLANSKYPASFLSDNLSIQDNVIQSAAAAGVKKLLFVSSSCVYPRL
CCCEEEEEEHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ASQPIEEDALLTGALEPTNRWYGVAKIAGMMQCAAYREQYGCDFIAAVPGNLYGPGDYFD
HCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
KENSHVIPAFLRRFHDAVTTGADEVAVWGSGTARREFVFVDECADALVFLLRSYSSGEIV
CCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEE
NIGSGVDVSISELAHLVADVTGFRGRIAFDTSQPEGAPRRLLSTKRLGELGWQSQMQLRE
EECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GLSRTYQWFLDNHSAGTVLRGYETADTSRDVDTIRTR
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]