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The map label for this gene is gatA [H]

Identifier: 86748609

GI number: 86748609

Start: 1673415

End: 1674797

Strand: Reverse

Name: gatA [H]

Synonym: RPB_1484

Alternate gene names: 86748609

Gene position: 1674797-1673415 (Counterclockwise)

Preceding gene: 86748610

Following gene: 86748608

Centisome position: 31.41

GC content: 69.2

Gene sequence:

>1383_bases
ATGACTCTGGCCGATTTGGACTTCAGCTTCGCCTCGATCCGCCGCGCCATGGCCGCCGGCGCCAAAGCTCCCGCGATCAT
CGCCGAAGCCTGCCGCCGCGCGCAGGCCGCCGCCGGCGAGGGCGTCTTCACGCTGCTGGTCGACCCCGCGGCGGCCATCG
CGCGCGCCGAGGAGTTGCAGGCGCGCGCCGATGCCGGCGCCGTCTTGCCGCTGCTCGGGCTGCCGTTCTCGGTCAAGGAC
AGCATCCATGTCGCCGGACTGCCGACCACCTCCAATTGCCCGGCGATGCGGCTCGAGCCGAAAGACTCCGCGCATGCCGT
GACACTGCTCGAACAGGCCGGCGCGGTGCTGATCGGCAAGAACACGCTGGATCAGTTCGCCACCGGGCTGAACGGCACCC
GCAGCCCGGATCCGCTGTGCCGCAACGCCATCGACCCGGCCTACATCCCCGGCGGCTCCAGCTCCGGCTCCGCGGTCGCG
GTGGCGCGCGACATCGTGGCGTTCTCGCTCGGCACCGACACCGGCGGCTCCGGCCGGGTGCCGGCGGCCTGCAACGGCAT
CGTCGGGCTGAAGCCGACCATCGGTTTGGTGAGCTCGCGTGGCCTCGTCTACAACAACCGCTCGTTCGACTGCATCCCGG
TGTTCTGCAAATTCGCCGACGACGCCTACGAGATCCTCGAAAACATCGCCGGCTTCGACGTGCTGGATCCGATGAGCCGA
ACCGATGCCGGCGCAATCGAGCTGCGCCCGCGTGCGCCGCAACCGCTGACCATCGCGATTCCGCGCGCCGATCAGCTGAC
TTTCTTCGGCGACACCGAGGCGGCCCGCGCCTACGAGGCCGATCTGGCGGTGCTGCAAAGCCTCGGCCACCGGCTGAAGC
CGGTCGACTTCACACTGTTCGAGACGGCGGGGCGGCTGGTGTTCCAAAGCGCGATGGTGGCGGAGCGGCTGATCGACTGC
GCCGACGTGGTGCGCGATCATCCCGAAGCGATTCATCCGGCAGTGTGGAGCGCATTGCAGCCCGGCCTCAGCTACACCGC
CCGCGACGCCTTCCAGGCGCTGTACGAGATCAAACAGGCGCAGCGCCAGGCCAGCCTGCTGCTGGCGGGTTGCGACGCCA
TGGTGGTCCCGACGGTGCCGACGATTTTCACCATCGCGCAGATGCTGGCCGATCCGATGGCGCTCAACAGCGCGATGGGC
ACCTACACTTATTTCGTCAATCCGCTCGACATGTGCGCGGTGGCGATCCCCGGCCGGACCCGCTCCGACGGCCTGCGCTC
GTCGCTGTGCTTCGTCGGCCGCGCCGGCGACGACGCGTTGCTGCGCACGCTGGCGCGCGGCTTCGAACAGGCGATGGTGC
CGGCGCTGGCTGTAGCCAGCTGA

Upstream 100 bases:

>100_bases
CTGCTCACCATGGCGATGAGCATCGCCATCATCACGGCCAGCCTGGCCGTCTGGAAAAAAGCCGTCGCCTGAGACGGCGC
CACCAAGAGGAAGACAGCGC

Downstream 100 bases:

>100_bases
CGCAGTCGGCACGACATGACTACACGACGGGCAGCGGCTGATGATTTTTCAGCCCTGCGGCAGGCGCGACGCGGTGGATT
AATTGCTTGAGCGTTCCACG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTLADLDFSFASIRRAMAAGAKAPAIIAEACRRAQAAAGEGVFTLLVDPAAAIARAEELQARADAGAVLPLLGLPFSVKD
SIHVAGLPTTSNCPAMRLEPKDSAHAVTLLEQAGAVLIGKNTLDQFATGLNGTRSPDPLCRNAIDPAYIPGGSSSGSAVA
VARDIVAFSLGTDTGGSGRVPAACNGIVGLKPTIGLVSSRGLVYNNRSFDCIPVFCKFADDAYEILENIAGFDVLDPMSR
TDAGAIELRPRAPQPLTIAIPRADQLTFFGDTEAARAYEADLAVLQSLGHRLKPVDFTLFETAGRLVFQSAMVAERLIDC
ADVVRDHPEAIHPAVWSALQPGLSYTARDAFQALYEIKQAQRQASLLLAGCDAMVVPTVPTIFTIAQMLADPMALNSAMG
TYTYFVNPLDMCAVAIPGRTRSDGLRSSLCFVGRAGDDALLRTLARGFEQAMVPALAVAS

Sequences:

>Translated_460_residues
MTLADLDFSFASIRRAMAAGAKAPAIIAEACRRAQAAAGEGVFTLLVDPAAAIARAEELQARADAGAVLPLLGLPFSVKD
SIHVAGLPTTSNCPAMRLEPKDSAHAVTLLEQAGAVLIGKNTLDQFATGLNGTRSPDPLCRNAIDPAYIPGGSSSGSAVA
VARDIVAFSLGTDTGGSGRVPAACNGIVGLKPTIGLVSSRGLVYNNRSFDCIPVFCKFADDAYEILENIAGFDVLDPMSR
TDAGAIELRPRAPQPLTIAIPRADQLTFFGDTEAARAYEADLAVLQSLGHRLKPVDFTLFETAGRLVFQSAMVAERLIDC
ADVVRDHPEAIHPAVWSALQPGLSYTARDAFQALYEIKQAQRQASLLLAGCDAMVVPTVPTIFTIAQMLADPMALNSAMG
TYTYFVNPLDMCAVAIPGRTRSDGLRSSLCFVGRAGDDALLRTLARGFEQAMVPALAVAS
>Mature_459_residues
TLADLDFSFASIRRAMAAGAKAPAIIAEACRRAQAAAGEGVFTLLVDPAAAIARAEELQARADAGAVLPLLGLPFSVKDS
IHVAGLPTTSNCPAMRLEPKDSAHAVTLLEQAGAVLIGKNTLDQFATGLNGTRSPDPLCRNAIDPAYIPGGSSSGSAVAV
ARDIVAFSLGTDTGGSGRVPAACNGIVGLKPTIGLVSSRGLVYNNRSFDCIPVFCKFADDAYEILENIAGFDVLDPMSRT
DAGAIELRPRAPQPLTIAIPRADQLTFFGDTEAARAYEADLAVLQSLGHRLKPVDFTLFETAGRLVFQSAMVAERLIDCA
DVVRDHPEAIHPAVWSALQPGLSYTARDAFQALYEIKQAQRQASLLLAGCDAMVVPTVPTIFTIAQMLADPMALNSAMGT
YTYFVNPLDMCAVAIPGRTRSDGLRSSLCFVGRAGDDALLRTLARGFEQAMVPALAVAS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=27.3622047244095, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI195972892, Length=354, Percent_Identity=23.728813559322, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=201, Percent_Identity=37.8109452736318, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=469, Percent_Identity=21.7484008528785, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=316, Percent_Identity=27.2151898734177, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556264, Length=129, Percent_Identity=36.4341085271318, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=37.3737373737374, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323950, Length=238, Percent_Identity=34.0336134453782, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648113, Length=277, Percent_Identity=32.4909747292419, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356731, Length=178, Percent_Identity=34.2696629213483, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652985, Length=168, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922090, Length=168, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652981, Length=168, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652983, Length=168, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48308; Mature: 48177

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLADLDFSFASIRRAMAAGAKAPAIIAEACRRAQAAAGEGVFTLLVDPAAAIARAEELQ
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
ARADAGAVLPLLGLPFSVKDSIHVAGLPTTSNCPAMRLEPKDSAHAVTLLEQAGAVLIGK
HHHCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCEEEEEC
NTLDQFATGLNGTRSPDPLCRNAIDPAYIPGGSSSGSAVAVARDIVAFSLGTDTGGSGRV
CHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCC
PAACNGIVGLKPTIGLVSSRGLVYNNRSFDCIPVFCKFADDAYEILENIAGFDVLDPMSR
CHHHCCCCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
TDAGAIELRPRAPQPLTIAIPRADQLTFFGDTEAARAYEADLAVLQSLGHRLKPVDFTLF
CCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ETAGRLVFQSAMVAERLIDCADVVRDHPEAIHPAVWSALQPGLSYTARDAFQALYEIKQA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QRQASLLLAGCDAMVVPTVPTIFTIAQMLADPMALNSAMGTYTYFVNPLDMCAVAIPGRT
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHCHHHHHHHHCCCEEEECHHHHHEEECCCCC
RSDGLRSSLCFVGRAGDDALLRTLARGFEQAMVPALAVAS
CCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLADLDFSFASIRRAMAAGAKAPAIIAEACRRAQAAAGEGVFTLLVDPAAAIARAEELQ
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
ARADAGAVLPLLGLPFSVKDSIHVAGLPTTSNCPAMRLEPKDSAHAVTLLEQAGAVLIGK
HHHCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCEEEEEC
NTLDQFATGLNGTRSPDPLCRNAIDPAYIPGGSSSGSAVAVARDIVAFSLGTDTGGSGRV
CHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCC
PAACNGIVGLKPTIGLVSSRGLVYNNRSFDCIPVFCKFADDAYEILENIAGFDVLDPMSR
CHHHCCCCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
TDAGAIELRPRAPQPLTIAIPRADQLTFFGDTEAARAYEADLAVLQSLGHRLKPVDFTLF
CCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ETAGRLVFQSAMVAERLIDCADVVRDHPEAIHPAVWSALQPGLSYTARDAFQALYEIKQA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QRQASLLLAGCDAMVVPTVPTIFTIAQMLADPMALNSAMGTYTYFVNPLDMCAVAIPGRT
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHCHHHHHHHHCCCEEEECHHHHHEEECCCCC
RSDGLRSSLCFVGRAGDDALLRTLARGFEQAMVPALAVAS
CCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA