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The map label for this gene is lhgO [H]

Identifier: 86748576

GI number: 86748576

Start: 1636951

End: 1638093

Strand: Reverse

Name: lhgO [H]

Synonym: RPB_1451

Alternate gene names: 86748576

Gene position: 1638093-1636951 (Counterclockwise)

Preceding gene: 86748578

Following gene: 86748574

Centisome position: 30.72

GC content: 70.25

Gene sequence:

>1143_bases
ATGGAACAGGTTGAATGCGTCGTGGTCGGCGCCGGCGTGGTCGGGCTCGCGATCGCGCGCCGGCTGGCGCAAGCGGGACG
CGAAGTCATCGTGCTGGAAGCCGCCGAGGGGATCGGCACCGGCACCTCGTCGCGCAACAGCGAGGTGATCCACGCCGGGA
TCTATTATCGCGCCGGCAGCCTGATGGCGCGGCTCTGCGTCGACGGCCGGCGGGCGCTGTATGCGTTTTGTCGAGACCAC
GGTGTGCCGCATCGGCAATGCGGCAAGCTGATCGTGGCGACCACAGCGGCGGAAGCCGAGAAGCTGGCATCGATCCGCGC
CCACGCCGCAGCCAACGGCGTCGACGATCTGCGCGCGCTCGACGGCGCCGAAGCCCGCGCGATGGAGCCGGCGCTCACTT
GCGTCGCCGCGCTGCTGTCGCCCTCGACCGGCATCGTCGACAGCCACGCCTACATGCTGGCGCTGTGCGGCGACGCCGAA
GCGGCCGGCGCGGCGTTCGCCTTCCACGCACCACTGCTGCGGGCCACGGCGGAGGCCGGCGGCTTCGCGCTCGAGGTCGG
CGGCGAGGCGCCGATGACGCTCGGCTGCCGGCTGCTGATCAATGCCGCGGGGCTCGCGGCCCCCGCGGTGGCGCGCGCGA
TCACGCCGATGCCGGCTGCGCTGGTGCCGTCGGCGTATCTCGCCAAGGGCAATTACTTCACCTGCAATGCGCGGGCACCG
TTCTCACACCTGATCTATCCGGTGCCCGAGCCCGGCGGGCTCGGCGTACATCTGACGCTGGATCTGGCGGGACAGGCCAA
GTTCGGCCCGGACGTCGAATGGATCGACACCGTCGACTACGTGGTCGATCCGGCGCGGGCGGAGCGGTTCTATCCGGCGA
TTCGCCGATACTGGCCGGGCCTTCCGGACGGGGCGCTGGCGCCGGGCTATTCCGGGATCAGGCCGAAGATCGTGCCGCCT
GCGGTCGCGGTGCAGGACTTCGTCATCCAGGGACCGGATGTCCACGGCGTCGCCGGGCTGATCAACCTGTTCGGCATCGA
ATCGCCGGGACTGACCGCGTCGCTGGCGATCGCCGAGGACGTCGCCCAGTTGTCCGACAATGTGTCGGCCGGTTTGTCGG
CTGATGTGGCGGCAAGTGCGTAG

Upstream 100 bases:

>100_bases
GCGTGGCTATTGACCTGAATTTCATCAATTGACCTGTGATGGTTAATGCCGCGATAACACGGCGCTTTCGGGCTCGCGCC
CGTTCGGGGACCGAGGGACA

Downstream 100 bases:

>100_bases
GCACAATGCATCGGCCTGTGTGGCGGCCCGCACCTGAGCGATTCAACTCAGGCGCCCGACAATCTCTGCGCTCCCGGATA
CGTCCGAGGACTGCGCTGCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MEQVECVVVGAGVVGLAIARRLAQAGREVIVLEAAEGIGTGTSSRNSEVIHAGIYYRAGSLMARLCVDGRRALYAFCRDH
GVPHRQCGKLIVATTAAEAEKLASIRAHAAANGVDDLRALDGAEARAMEPALTCVAALLSPSTGIVDSHAYMLALCGDAE
AAGAAFAFHAPLLRATAEAGGFALEVGGEAPMTLGCRLLINAAGLAAPAVARAITPMPAALVPSAYLAKGNYFTCNARAP
FSHLIYPVPEPGGLGVHLTLDLAGQAKFGPDVEWIDTVDYVVDPARAERFYPAIRRYWPGLPDGALAPGYSGIRPKIVPP
AVAVQDFVIQGPDVHGVAGLINLFGIESPGLTASLAIAEDVAQLSDNVSAGLSADVAASA

Sequences:

>Translated_380_residues
MEQVECVVVGAGVVGLAIARRLAQAGREVIVLEAAEGIGTGTSSRNSEVIHAGIYYRAGSLMARLCVDGRRALYAFCRDH
GVPHRQCGKLIVATTAAEAEKLASIRAHAAANGVDDLRALDGAEARAMEPALTCVAALLSPSTGIVDSHAYMLALCGDAE
AAGAAFAFHAPLLRATAEAGGFALEVGGEAPMTLGCRLLINAAGLAAPAVARAITPMPAALVPSAYLAKGNYFTCNARAP
FSHLIYPVPEPGGLGVHLTLDLAGQAKFGPDVEWIDTVDYVVDPARAERFYPAIRRYWPGLPDGALAPGYSGIRPKIVPP
AVAVQDFVIQGPDVHGVAGLINLFGIESPGLTASLAIAEDVAQLSDNVSAGLSADVAASA
>Mature_380_residues
MEQVECVVVGAGVVGLAIARRLAQAGREVIVLEAAEGIGTGTSSRNSEVIHAGIYYRAGSLMARLCVDGRRALYAFCRDH
GVPHRQCGKLIVATTAAEAEKLASIRAHAAANGVDDLRALDGAEARAMEPALTCVAALLSPSTGIVDSHAYMLALCGDAE
AAGAAFAFHAPLLRATAEAGGFALEVGGEAPMTLGCRLLINAAGLAAPAVARAITPMPAALVPSAYLAKGNYFTCNARAP
FSHLIYPVPEPGGLGVHLTLDLAGQAKFGPDVEWIDTVDYVVDPARAERFYPAIRRYWPGLPDGALAPGYSGIRPKIVPP
AVAVQDFVIQGPDVHGVAGLINLFGIESPGLTASLAIAEDVAQLSDNVSAGLSADVAASA

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=266, Percent_Identity=30.8270676691729, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI87082148, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17565764, Length=281, Percent_Identity=33.8078291814947, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585081, Length=385, Percent_Identity=29.0909090909091, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 39020; Mature: 39020

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVECVVVGAGVVGLAIARRLAQAGREVIVLEAAEGIGTGTSSRNSEVIHAGIYYRAGS
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHH
LMARLCVDGRRALYAFCRDHGVPHRQCGKLIVATTAAEAEKLASIRAHAAANGVDDLRAL
HHHHHHHCCHHHHHHHHHHCCCCHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHC
DGAEARAMEPALTCVAALLSPSTGIVDSHAYMLALCGDAEAAGAAFAFHAPLLRATAEAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
GFALEVGGEAPMTLGCRLLINAAGLAAPAVARAITPMPAALVPSAYLAKGNYFTCNARAP
CEEEEECCCCCHHHHHEEEEECCCHHHHHHHHHHCCCCHHHCCHHHEECCCEEEEECCCC
FSHLIYPVPEPGGLGVHLTLDLAGQAKFGPDVEWIDTVDYVVDPARAERFYPAIRRYWPG
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LPDGALAPGYSGIRPKIVPPAVAVQDFVIQGPDVHGVAGLINLFGIESPGLTASLAIAED
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHH
VAQLSDNVSAGLSADVAASA
HHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEQVECVVVGAGVVGLAIARRLAQAGREVIVLEAAEGIGTGTSSRNSEVIHAGIYYRAGS
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHH
LMARLCVDGRRALYAFCRDHGVPHRQCGKLIVATTAAEAEKLASIRAHAAANGVDDLRAL
HHHHHHHCCHHHHHHHHHHCCCCHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHC
DGAEARAMEPALTCVAALLSPSTGIVDSHAYMLALCGDAEAAGAAFAFHAPLLRATAEAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
GFALEVGGEAPMTLGCRLLINAAGLAAPAVARAITPMPAALVPSAYLAKGNYFTCNARAP
CEEEEECCCCCHHHHHEEEEECCCHHHHHHHHHHCCCCHHHCCHHHEECCCEEEEECCCC
FSHLIYPVPEPGGLGVHLTLDLAGQAKFGPDVEWIDTVDYVVDPARAERFYPAIRRYWPG
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LPDGALAPGYSGIRPKIVPPAVAVQDFVIQGPDVHGVAGLINLFGIESPGLTASLAIAED
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHH
VAQLSDNVSAGLSADVAASA
HHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]