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The map label for this gene is yegE [H]

Identifier: 86748573

GI number: 86748573

Start: 1635128

End: 1635886

Strand: Direct

Name: yegE [H]

Synonym: RPB_1448

Alternate gene names: 86748573

Gene position: 1635128-1635886 (Clockwise)

Preceding gene: 86748569

Following gene: 86748575

Centisome position: 30.67

GC content: 70.75

Gene sequence:

>759_bases
ATGAAAAAGACGCCTCCCCGATCTCCAAAGGCATCCCGGACCGGCCACAGGCCCGCGGCACGGCCCGTTGCCGGGAAGCG
CCCGCTGGCGGCGTCCGGTCCGACCAAGGCGCTGCTGGTGAAGGCGCTTCCGGTCAAGGCCATGCCGCGCGCGCGCGTCG
GTTCCGGCCGGCTGCCGGCGGACGCCCAGGGGAAGATCCGGCGGCTGAAGGCGCAGCTCGCCGCGGCGCTGGCGCAGATC
GACGAATTGCGCGCCTCCGCCGAGACCGATTTCCTGCTCGGCATCTCCAATCGCCGCGGCTTCGAGCGCGAACTCGCCCG
CGCCCTCGCTTACATCAAGCGCTACGGCGCCGGCGGTGCGCTGATCATGCTCGACGTCGACCGGCTGAAGCCGGTCAACG
ATACTTACGGTCACGCCGCCGGCGACGTCGTGCTGAAAGCGGTGGTCGACGCGCTGCTCCGCCATGTCCGCTCGTCCGAT
CTGATCGGCCGGCTCGGCGGCGACGAGTTCGCGCTGCTGCTGTGGAACCTCGGCGAGGCCGACGCCCGGCTCAAGGCCGC
GGCGCTGGAGCAGGCGGTGGATCAACTCGCGATTCAATACGGCGCACACACCGTCACCGCCGGAATCTCCGCCGGCGTGG
CAATGCTGACGGCGGACATGGATGTGGTGGAAGCGCTCGCCGTCGCCGACCGGGCGATGTACGCCCGCAAGGCCGAGCGT
CACGCGCTGTCGGGGCAGCCGGAGCCGCTCAGGCGATGA

Upstream 100 bases:

>100_bases
GCCGCGGCCTCGGCCAAGGCGTCGGGAATGCACTATCCTTTCTTGGTGAAGACCGGCAAGTGTTGTGCTGTGTGTTGTCG
TGTTTCTCGCGCGGATTCTG

Downstream 100 bases:

>100_bases
TGTCGGGCGTGATCTGATCTTCGATGAAGGCGATGCGGTCGCGCAGCGCCAGCTTGCGCTTTTTCAGCCGCTGCAACCGC
AGCAGATCGGGCGCCGGTGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKKTPPRSPKASRTGHRPAARPVAGKRPLAASGPTKALLVKALPVKAMPRARVGSGRLPADAQGKIRRLKAQLAAALAQI
DELRASAETDFLLGISNRRGFERELARALAYIKRYGAGGALIMLDVDRLKPVNDTYGHAAGDVVLKAVVDALLRHVRSSD
LIGRLGGDEFALLLWNLGEADARLKAAALEQAVDQLAIQYGAHTVTAGISAGVAMLTADMDVVEALAVADRAMYARKAER
HALSGQPEPLRR

Sequences:

>Translated_252_residues
MKKTPPRSPKASRTGHRPAARPVAGKRPLAASGPTKALLVKALPVKAMPRARVGSGRLPADAQGKIRRLKAQLAAALAQI
DELRASAETDFLLGISNRRGFERELARALAYIKRYGAGGALIMLDVDRLKPVNDTYGHAAGDVVLKAVVDALLRHVRSSD
LIGRLGGDEFALLLWNLGEADARLKAAALEQAVDQLAIQYGAHTVTAGISAGVAMLTADMDVVEALAVADRAMYARKAER
HALSGQPEPLRR
>Mature_252_residues
MKKTPPRSPKASRTGHRPAARPVAGKRPLAASGPTKALLVKALPVKAMPRARVGSGRLPADAQGKIRRLKAQLAAALAQI
DELRASAETDFLLGISNRRGFERELARALAYIKRYGAGGALIMLDVDRLKPVNDTYGHAAGDVVLKAVVDALLRHVRSSD
LIGRLGGDEFALLLWNLGEADARLKAAALEQAVDQLAIQYGAHTVTAGISAGVAMLTADMDVVEALAVADRAMYARKAER
HALSGQPEPLRR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=157, Percent_Identity=36.9426751592357, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=30.6818181818182, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=34.1614906832298, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=119, Percent_Identity=37.8151260504202, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.2993630573248, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787262, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=33.1288343558282, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=30.8108108108108, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=27.6729559748428, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=30.379746835443, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=28.9156626506024, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 26811; Mature: 26811

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTPPRSPKASRTGHRPAARPVAGKRPLAASGPTKALLVKALPVKAMPRARVGSGRLPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DAQGKIRRLKAQLAAALAQIDELRASAETDFLLGISNRRGFERELARALAYIKRYGAGGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
LIMLDVDRLKPVNDTYGHAAGDVVLKAVVDALLRHVRSSDLIGRLGGDEFALLLWNLGEA
EEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
DARLKAAALEQAVDQLAIQYGAHTVTAGISAGVAMLTADMDVVEALAVADRAMYARKAER
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALSGQPEPLRR
HHCCCCCCCCCC
>Mature Secondary Structure
MKKTPPRSPKASRTGHRPAARPVAGKRPLAASGPTKALLVKALPVKAMPRARVGSGRLPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DAQGKIRRLKAQLAAALAQIDELRASAETDFLLGISNRRGFERELARALAYIKRYGAGGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
LIMLDVDRLKPVNDTYGHAAGDVVLKAVVDALLRHVRSSDLIGRLGGDEFALLLWNLGEA
EEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
DARLKAAALEQAVDQLAIQYGAHTVTAGISAGVAMLTADMDVVEALAVADRAMYARKAER
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALSGQPEPLRR
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]