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The map label for this gene is ybiS [H]

Identifier: 86748516

GI number: 86748516

Start: 1582513

End: 1583271

Strand: Direct

Name: ybiS [H]

Synonym: RPB_1391

Alternate gene names: 86748516

Gene position: 1582513-1583271 (Clockwise)

Preceding gene: 86748514

Following gene: 86748517

Centisome position: 29.68

GC content: 67.06

Gene sequence:

>759_bases
ATGATGATTTCGGGTATCGCTTCGAAACGCAATTCGGTGGCGCGTCCACTGATGGCGTTCGCTGCCGCGCTCCTTCTGAC
GGTCGCGCTACCCGGGACGGCGTCGGCGCAGAGGCTCGGCTACGCGGCCGCGCCTCAGGCCGGCCCGTTCGGCGCCGGCG
ACCAGGTGCTGTCCTCTCCGCAACAGGACACCGCACCGGATGCGACGGTGACGGACGATGAGGACGCGCAATTGTCCGAC
CGGCTGCGGCGGCAGGTGGTGGCCTACAATATCAACCAGCCCGCCGGCACCATCGTGATCGATACCGCCAACACCTATTT
ATACTACCTGCTGGGCGGCGGCCGGGCGATCCGCTACGGCGTCGGCGTCGGTCGCGAGGGTTTCACCTGGGCGGGGACGC
AGACGATCACCCGCAAGGCGGAGTGGCCGGATTGGCATCCGCCGGCGCAGATGATCGCGCGTCAGCCCTATCTGCCGCGC
TTCATGGCCGGCGGACCCGGCAATCCGCTGGGCGCGCGGGCAATGTATCTCGGCTCGACCGAATACCGCATCCACGGCAC
CAACGATCCGTCGACAATCGGCAAGTTCGTGTCGTCGGGATGTATCCGGTTGACCAATGAGGACGTCGCCGACCTGTTCA
GCCGCGCCACCGTCGGCACACGGGTGGTGGTGCTGCCGAAGTCGGCGCCGAGCCTGGAAACCCGCAAGCCACGACCCGAG
CGGCAGGCCATGAACATTGCCGTGTCGTCGCGCGACTGA

Upstream 100 bases:

>100_bases
GGCTTGTCAAGAGCCTTCCTGCGACGCGCCGGAACCGGATGGTCGACGCCGCGTTTGTTAGCAGCATCGCGACGATGCGA
CGACATGCGGGGACTGCACC

Downstream 100 bases:

>100_bases
TCAGGAAGGAGGCCGCGATGCGTCGCTCAATGTCAAAAGCCGTCGGGATCGGCGCGGTGGCCATGCTGGCGCTGGCTGGC
ATGGCGTCCGGCGCGCAGGC

Product: ErfK/YbiS/YcfS/YnhG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MMISGIASKRNSVARPLMAFAAALLLTVALPGTASAQRLGYAAAPQAGPFGAGDQVLSSPQQDTAPDATVTDDEDAQLSD
RLRRQVVAYNINQPAGTIVIDTANTYLYYLLGGGRAIRYGVGVGREGFTWAGTQTITRKAEWPDWHPPAQMIARQPYLPR
FMAGGPGNPLGARAMYLGSTEYRIHGTNDPSTIGKFVSSGCIRLTNEDVADLFSRATVGTRVVVLPKSAPSLETRKPRPE
RQAMNIAVSSRD

Sequences:

>Translated_252_residues
MMISGIASKRNSVARPLMAFAAALLLTVALPGTASAQRLGYAAAPQAGPFGAGDQVLSSPQQDTAPDATVTDDEDAQLSD
RLRRQVVAYNINQPAGTIVIDTANTYLYYLLGGGRAIRYGVGVGREGFTWAGTQTITRKAEWPDWHPPAQMIARQPYLPR
FMAGGPGNPLGARAMYLGSTEYRIHGTNDPSTIGKFVSSGCIRLTNEDVADLFSRATVGTRVVVLPKSAPSLETRKPRPE
RQAMNIAVSSRD
>Mature_252_residues
MMISGIASKRNSVARPLMAFAAALLLTVALPGTASAQRLGYAAAPQAGPFGAGDQVLSSPQQDTAPDATVTDDEDAQLSD
RLRRQVVAYNINQPAGTIVIDTANTYLYYLLGGGRAIRYGVGVGREGFTWAGTQTITRKAEWPDWHPPAQMIARQPYLPR
FMAGGPGNPLGARAMYLGSTEYRIHGTNDPSTIGKFVSSGCIRLTNEDVADLFSRATVGTRVVVLPKSAPSLETRKPRPE
RQAMNIAVSSRD

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=135, Percent_Identity=42.2222222222222, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788299, Length=137, Percent_Identity=38.6861313868613, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787968, Length=140, Percent_Identity=37.1428571428571, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787356, Length=237, Percent_Identity=30.379746835443, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 26964; Mature: 26964

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMISGIASKRNSVARPLMAFAAALLLTVALPGTASAQRLGYAAAPQAGPFGAGDQVLSSP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCC
QQDTAPDATVTDDEDAQLSDRLRRQVVAYNINQPAGTIVIDTANTYLYYLLGGGRAIRYG
CCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCEEEEECCCEEEEEEEECCEEEEEE
VGVGREGFTWAGTQTITRKAEWPDWHPPAQMIARQPYLPRFMAGGPGNPLGARAMYLGST
CCCCCCCCEECCCHHEECCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCC
EYRIHGTNDPSTIGKFVSSGCIRLTNEDVADLFSRATVGTRVVVLPKSAPSLETRKPRPE
EEEEECCCCHHHHHHHHHCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
RQAMNIAVSSRD
HHHEEEEEECCC
>Mature Secondary Structure
MMISGIASKRNSVARPLMAFAAALLLTVALPGTASAQRLGYAAAPQAGPFGAGDQVLSSP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCC
QQDTAPDATVTDDEDAQLSDRLRRQVVAYNINQPAGTIVIDTANTYLYYLLGGGRAIRYG
CCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCEEEEECCCEEEEEEEECCEEEEEE
VGVGREGFTWAGTQTITRKAEWPDWHPPAQMIARQPYLPRFMAGGPGNPLGARAMYLGST
CCCCCCCCEECCCHHEECCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCC
EYRIHGTNDPSTIGKFVSSGCIRLTNEDVADLFSRATVGTRVVVLPKSAPSLETRKPRPE
EEEEECCCCHHHHHHHHHCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
RQAMNIAVSSRD
HHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]