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The map label for this gene is dacF [H]

Identifier: 86748470

GI number: 86748470

Start: 1531960

End: 1533459

Strand: Reverse

Name: dacF [H]

Synonym: RPB_1345

Alternate gene names: 86748470

Gene position: 1533459-1531960 (Counterclockwise)

Preceding gene: 86748471

Following gene: 86748469

Centisome position: 28.76

GC content: 67.8

Gene sequence:

>1500_bases
GTGCAATTTTTCACCGCGCAGCCTCGCAGCCCTTCGCTGTTCTGGATCACCCTCACCGCCCTGCTGATGCTGACGGCTCT
GCCGCGGATCGCCTCGGCGGAAGCTCTGCTGCTGGTCGAGGCCGATACCGGCAAGGTGCTGCAGGCGGAGAACGCCACCT
ATCCATGGTATCCCGCTTCGGTCACTAAGATCATGACCGCCTATGTGACGCTGAAGGCGGTGAAGGAAGGCCGGCTGACG
CTCGACTCGCTGTTGACCGTGTCGCCGGTCGCCGCGGCGCAATCGCCGTCGAAGATGGGCTTCGCCCCCGGCACGCTGAT
CACCGTCGACAATGCGCTGAAGATGATGATGGTGAAGTCGGCCAACGACATCGCCGTGGTGCTGGCCGAAGGCGTCGGCG
GCTCGGTCGACGGCTTCTCGGACATGATGAACACGACCGCGCAGCGGCTCGGCATGACCCAGACTCACTACGTCAATCCG
AACGGACTGCCGGCCGAAGGCCAGGTCACCTCGGCGCGCGATCTGGCGATCCTGGCGCGCGCGGTGTTGCGCGACCTGCC
GGAATACGAGCACTTCATGCACATCCCGGCGATCCGTTTCGGCAAGCGTGTCACCCACAATTTCAACAAGCTGATCGGCC
GCTATCCCGGCGCCGACGGCTTCAAGACCGGCTTCATCTGCGCCTCTGGCTACAATCTGGTCGCCAGCGCCACCCGCAAT
GGCCGACGCCTGATCGCGGTGGTGCTCGGCGCCAATTCCGGGCAGGACCGCGCGGTCAAGGCGGCTCAACTGCTCGAACG
CGGCTTCTCGGCCAACACGCTGGCATGGCTGACGCCGTCGCTCGGCACCGTCGACAACCTCGCGCCGATCGACGCCACCC
CGCCCAACCTGCGCGACGAGATGTGCGGCCCCAAGCGCAAGCGCCCGGCCAGCGACGAGGACGAGCAGGTCGCCAGCGTC
ATCGCCAATGGCGACACGATGCTGTCGATGTTCTCGCCGCCGGCGCTGCGCCCGGTCGACATGATCGCCGCCGCGCCCGC
CCCGACCGAACCGGTCCAGGTCTACACCGGGCCGACCCGGACCGGCGCAGCCTTGATCGCGGCCGCCGCCAGCGACGCCG
AGCGCCAGGCCGCCGCCGGCAAGAAGAAGCTCGCCAAAAAGAAGAATGCGAAGCCGAAAGACGACGTCAAGGATGCCGCC
CTCGGCGAAAAGCACAACGGCCGTACGACGGTCACGATCGATACCAGCAAGCCCAAACCCGCCGCTGCCAAACCTGACGT
AGCCAGGCCCGCGGCTGCCAAGCCCGCCGCTACCAAGCCCGCCACCGCCAAGCCTGCCAATGCCAAGCCTGCCGGCGCCA
AAGCAGCCAACGCAAAGCCAGCCGCCAAGCCCGAGACGCCGGCCGCGGCCGACAAGCAGGCGCCCCCGGCCAAACCCAAG
GCGGCGGCCGCCAAGCCGGGACCGAAAGCCGCGACCGGCGAGACCAGGCCCGGCACCTGA

Upstream 100 bases:

>100_bases
GGTATAGGGTCGGGTCTGCTGGGCCAACGCCTGAACCCGAACGTCAACCCGTAAAGACCTCGTCCGCAACGGACGGATCT
CAAAGGACCGGAATCGGATC

Downstream 100 bases:

>100_bases
GGGACATCCGGCTCGTCGGGCCGACTACCTGTATTAACCCCGAGTGTGGAGCGACCGCGTGCGGCGGCCGGTCAGCTTGC
GTTCGGCCACGGCTTCGCTC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MQFFTAQPRSPSLFWITLTALLMLTALPRIASAEALLLVEADTGKVLQAENATYPWYPASVTKIMTAYVTLKAVKEGRLT
LDSLLTVSPVAAAQSPSKMGFAPGTLITVDNALKMMMVKSANDIAVVLAEGVGGSVDGFSDMMNTTAQRLGMTQTHYVNP
NGLPAEGQVTSARDLAILARAVLRDLPEYEHFMHIPAIRFGKRVTHNFNKLIGRYPGADGFKTGFICASGYNLVASATRN
GRRLIAVVLGANSGQDRAVKAAQLLERGFSANTLAWLTPSLGTVDNLAPIDATPPNLRDEMCGPKRKRPASDEDEQVASV
IANGDTMLSMFSPPALRPVDMIAAAPAPTEPVQVYTGPTRTGAALIAAAASDAERQAAAGKKKLAKKKNAKPKDDVKDAA
LGEKHNGRTTVTIDTSKPKPAAAKPDVARPAAAKPAATKPATAKPANAKPAGAKAANAKPAAKPETPAAADKQAPPAKPK
AAAAKPGPKAATGETRPGT

Sequences:

>Translated_499_residues
MQFFTAQPRSPSLFWITLTALLMLTALPRIASAEALLLVEADTGKVLQAENATYPWYPASVTKIMTAYVTLKAVKEGRLT
LDSLLTVSPVAAAQSPSKMGFAPGTLITVDNALKMMMVKSANDIAVVLAEGVGGSVDGFSDMMNTTAQRLGMTQTHYVNP
NGLPAEGQVTSARDLAILARAVLRDLPEYEHFMHIPAIRFGKRVTHNFNKLIGRYPGADGFKTGFICASGYNLVASATRN
GRRLIAVVLGANSGQDRAVKAAQLLERGFSANTLAWLTPSLGTVDNLAPIDATPPNLRDEMCGPKRKRPASDEDEQVASV
IANGDTMLSMFSPPALRPVDMIAAAPAPTEPVQVYTGPTRTGAALIAAAASDAERQAAAGKKKLAKKKNAKPKDDVKDAA
LGEKHNGRTTVTIDTSKPKPAAAKPDVARPAAAKPAATKPATAKPANAKPAGAKAANAKPAAKPETPAAADKQAPPAKPK
AAAAKPGPKAATGETRPGT
>Mature_499_residues
MQFFTAQPRSPSLFWITLTALLMLTALPRIASAEALLLVEADTGKVLQAENATYPWYPASVTKIMTAYVTLKAVKEGRLT
LDSLLTVSPVAAAQSPSKMGFAPGTLITVDNALKMMMVKSANDIAVVLAEGVGGSVDGFSDMMNTTAQRLGMTQTHYVNP
NGLPAEGQVTSARDLAILARAVLRDLPEYEHFMHIPAIRFGKRVTHNFNKLIGRYPGADGFKTGFICASGYNLVASATRN
GRRLIAVVLGANSGQDRAVKAAQLLERGFSANTLAWLTPSLGTVDNLAPIDATPPNLRDEMCGPKRKRPASDEDEQVASV
IANGDTMLSMFSPPALRPVDMIAAAPAPTEPVQVYTGPTRTGAALIAAAASDAERQAAAGKKKLAKKKNAKPKDDVKDAA
LGEKHNGRTTVTIDTSKPKPAAAKPDVARPAAAKPAATKPATAKPANAKPAGAKAANAKPAAKPETPAAADKQAPPAKPK
AAAAKPGPKAATGETRPGT

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=245, Percent_Identity=39.1836734693878, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082022, Length=243, Percent_Identity=36.6255144032922, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1786851, Length=254, Percent_Identity=35.8267716535433, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87082054, Length=258, Percent_Identity=29.0697674418605, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52042; Mature: 52042

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFFTAQPRSPSLFWITLTALLMLTALPRIASAEALLLVEADTGKVLQAENATYPWYPAS
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCHH
VTKIMTAYVTLKAVKEGRLTLDSLLTVSPVAAAQSPSKMGFAPGTLITVDNALKMMMVKS
HHHHHHHHHHHHHHHCCCEEHHHHHHHCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCC
ANDIAVVLAEGVGGSVDGFSDMMNTTAQRLGMTQTHYVNPNGLPAEGQVTSARDLAILAR
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
AVLRDLPEYEHFMHIPAIRFGKRVTHNFNKLIGRYPGADGFKTGFICASGYNLVASATRN
HHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCC
GRRLIAVVLGANSGQDRAVKAAQLLERGFSANTLAWLTPSLGTVDNLAPIDATPPNLRDE
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH
MCGPKRKRPASDEDEQVASVIANGDTMLSMFSPPALRPVDMIAAAPAPTEPVQVYTGPTR
HCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEECCCC
TGAALIAAAASDAERQAAAGKKKLAKKKNAKPKDDVKDAALGEKHNGRTTVTIDTSKPKP
CCHHHEEEHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCC
AAAKPDVARPAAAKPAATKPATAKPANAKPAGAKAANAKPAAKPETPAAADKQAPPAKPK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAAKPGPKAATGETRPGT
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFFTAQPRSPSLFWITLTALLMLTALPRIASAEALLLVEADTGKVLQAENATYPWYPAS
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCHH
VTKIMTAYVTLKAVKEGRLTLDSLLTVSPVAAAQSPSKMGFAPGTLITVDNALKMMMVKS
HHHHHHHHHHHHHHHCCCEEHHHHHHHCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCC
ANDIAVVLAEGVGGSVDGFSDMMNTTAQRLGMTQTHYVNPNGLPAEGQVTSARDLAILAR
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
AVLRDLPEYEHFMHIPAIRFGKRVTHNFNKLIGRYPGADGFKTGFICASGYNLVASATRN
HHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCC
GRRLIAVVLGANSGQDRAVKAAQLLERGFSANTLAWLTPSLGTVDNLAPIDATPPNLRDE
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH
MCGPKRKRPASDEDEQVASVIANGDTMLSMFSPPALRPVDMIAAAPAPTEPVQVYTGPTR
HCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEECCCC
TGAALIAAAASDAERQAAAGKKKLAKKKNAKPKDDVKDAALGEKHNGRTTVTIDTSKPKP
CCHHHEEEHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCC
AAAKPDVARPAAAKPAATKPATAKPANAKPAGAKAANAKPAAKPETPAAADKQAPPAKPK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAAKPGPKAATGETRPGT
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]