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The map label for this gene is purA [H]

Identifier: 86748455

GI number: 86748455

Start: 1516679

End: 1517971

Strand: Direct

Name: purA [H]

Synonym: RPB_1330

Alternate gene names: 86748455

Gene position: 1516679-1517971 (Clockwise)

Preceding gene: 86748449

Following gene: 86748456

Centisome position: 28.45

GC content: 67.75

Gene sequence:

>1293_bases
ATGGCCAATGTCGTCGTAGTCGGCGCCCAATGGGGCGACGAGGGGAAGGGCAAGATCGTCGATTGGCTGTCGGAGCAGGC
CGACATCGTCGCCCGCTTCCAGGGCGGGCACAATGCCGGCCACACGCTGGTGATCAACGGCGAGACCTACAAGCTGGCGC
TGCTGCCGTCGGGCGTGCTGCGGCCGTCGAAGCTGGCGGTGATCGGCAACGGCGTGGTGTTCGATCCGCAGGCGTTTCTC
GATGAGGTCGAGCGGCTGACCAAACAGGGCGTCGCGATCTCGCCGGAGAATCTGCGCGTCGCCGAGAACGTCACGCTGAT
CCTGCCGCTGCATCGCGAACTCGACGCGCTGCGCGAGAACGCCAGCAAGGCGACCGCGATCGGCACCACCCAGCGCGGCA
TCGGCCCGGCCTATGAAGACAAGGTCGGCCGCCGCGCGATCCGGCTGATGGATCTCGCCGACATCGACACGCTGCCGCAC
AAGATCGAGCGGCTGCTGACGCATCACAATGCGCTGCGCCGCGGTCTGGGCCTCGCCGAATTCGAGGCCGGCGCGATCCT
GAAGGAGCTGACCGCGCTGGCGCCGAAGCTCTTGCCCTATGCCGAGACGGTGTGGCGGCTGCTCGACATCAAGCGCCGCG
AAGGCAAGCGCATCCTGTTCGAGGGCGCGCAGGGCGCGCTGCTGGATGTGGATCACGGCACCTATCCCTACGTCACCTCG
TCGAACACGGTGGCGGCGCAGGCCGCGACCGGCACCGGCATGGGGCCGGGCTCGGTCGGCTATGTGCTCGGCATCTGCAA
GGCCTACACCACGCGCGTCGGCCAGGGGCCGTTCCCGACCGAACTGGACAACGAGATCGGCCGCAAGATCGGCGAGCGCG
GCCGCGAATTCGGCACCAACACCGGGCGGCCGCGCCGCTGCGGCTGGTTCGACGCGGTGCTGGTGCGCCAGACGGTGCGG
ACCTGCGGCATCCACGGCCTGGCGCTGACCAAGCTCGACATTCTCGACGGCTTCGAACAGATCGAGGTCTGCGTCGGCTA
TCGACTCGACGGCAAGGAGATCGACCATCTGCCGGCCGGCGAGGGCGCGCAGGCGCGCGTCGAGCCGATCTACGAGACCA
TCGAGGGCTGGAAGGAACCGACCGCCAATGCGCGGTCCTGGGCCGAGCTGCCGGCGCAGGCGATCAAATATGTTCGCCGC
ATCGAGGAGCTGGTCGGCTGTCCGGTGGCGCTGTTGTCCACCAGTCCGGAGCGCGAGGATACAATTCTGGTGCAGAATCC
CTTCGAGGCTTAA

Upstream 100 bases:

>100_bases
GCCGTGTTTTGGCTTGGCGCTCAGGACGATCCGGCCAGCCGCCGACAGGCGCTCGACGATCGTGCTTCATCCGGTTGAGT
TGCAGGGGAACTGCTCGAAA

Downstream 100 bases:

>100_bases
TAAGATGTTACCGAAAGGCATCGAAGCTTGAGTCAAGATGGCTGATTACTATCCGCTGATCGCACGCGCCATATCCGGCC
TGGACCCCAGTGCTCCGGGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVARFQGGHNAGHTLVINGETYKLALLPSGVLRPSKLAVIGNGVVFDPQAFL
DEVERLTKQGVAISPENLRVAENVTLILPLHRELDALRENASKATAIGTTQRGIGPAYEDKVGRRAIRLMDLADIDTLPH
KIERLLTHHNALRRGLGLAEFEAGAILKELTALAPKLLPYAETVWRLLDIKRREGKRILFEGAQGALLDVDHGTYPYVTS
SNTVAAQAATGTGMGPGSVGYVLGICKAYTTRVGQGPFPTELDNEIGRKIGERGREFGTNTGRPRRCGWFDAVLVRQTVR
TCGIHGLALTKLDILDGFEQIEVCVGYRLDGKEIDHLPAGEGAQARVEPIYETIEGWKEPTANARSWAELPAQAIKYVRR
IEELVGCPVALLSTSPEREDTILVQNPFEA

Sequences:

>Translated_430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVARFQGGHNAGHTLVINGETYKLALLPSGVLRPSKLAVIGNGVVFDPQAFL
DEVERLTKQGVAISPENLRVAENVTLILPLHRELDALRENASKATAIGTTQRGIGPAYEDKVGRRAIRLMDLADIDTLPH
KIERLLTHHNALRRGLGLAEFEAGAILKELTALAPKLLPYAETVWRLLDIKRREGKRILFEGAQGALLDVDHGTYPYVTS
SNTVAAQAATGTGMGPGSVGYVLGICKAYTTRVGQGPFPTELDNEIGRKIGERGREFGTNTGRPRRCGWFDAVLVRQTVR
TCGIHGLALTKLDILDGFEQIEVCVGYRLDGKEIDHLPAGEGAQARVEPIYETIEGWKEPTANARSWAELPAQAIKYVRR
IEELVGCPVALLSTSPEREDTILVQNPFEA
>Mature_429_residues
ANVVVVGAQWGDEGKGKIVDWLSEQADIVARFQGGHNAGHTLVINGETYKLALLPSGVLRPSKLAVIGNGVVFDPQAFLD
EVERLTKQGVAISPENLRVAENVTLILPLHRELDALRENASKATAIGTTQRGIGPAYEDKVGRRAIRLMDLADIDTLPHK
IERLLTHHNALRRGLGLAEFEAGAILKELTALAPKLLPYAETVWRLLDIKRREGKRILFEGAQGALLDVDHGTYPYVTSS
NTVAAQAATGTGMGPGSVGYVLGICKAYTTRVGQGPFPTELDNEIGRKIGERGREFGTNTGRPRRCGWFDAVLVRQTVRT
CGIHGLALTKLDILDGFEQIEVCVGYRLDGKEIDHLPAGEGAQARVEPIYETIEGWKEPTANARSWAELPAQAIKYVRRI
EELVGCPVALLSTSPEREDTILVQNPFEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=43.7647058823529, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=43.0260047281324, Blast_Score=341, Evalue=6e-94,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=40.8629441624365, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=51.1627906976744, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.9906542056075, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.9906542056075, Blast_Score=337, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=40.1392111368909, Blast_Score=316, Evalue=5e-87,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=41.371158392435, Blast_Score=322, Evalue=4e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46827; Mature: 46696

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKIVDWLSEQADIVARFQGGHNAGHTLVINGETYKLALLPSGVL
CCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCEEEEEECCCCCC
RPSKLAVIGNGVVFDPQAFLDEVERLTKQGVAISPENLRVAENVTLILPLHRELDALREN
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCEECCCCEEEECCEEEEEECHHHHHHHHHC
ASKATAIGTTQRGIGPAYEDKVGRRAIRLMDLADIDTLPHKIERLLTHHNALRRGLGLAE
CCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FEAGAILKELTALAPKLLPYAETVWRLLDIKRREGKRILFEGAQGALLDVDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVAAQAATGTGMGPGSVGYVLGICKAYTTRVGQGPFPTELDNEIGRKIGERGREFGTN
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TGRPRRCGWFDAVLVRQTVRTCGIHGLALTKLDILDGFEQIEVCVGYRLDGKEIDHLPAG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCEEECCCCCCCCCCC
EGAQARVEPIYETIEGWKEPTANARSWAELPAQAIKYVRRIEELVGCPVALLSTSPERED
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILVQNPFEA
EEEECCCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKIVDWLSEQADIVARFQGGHNAGHTLVINGETYKLALLPSGVL
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCEEEEEECCCCCC
RPSKLAVIGNGVVFDPQAFLDEVERLTKQGVAISPENLRVAENVTLILPLHRELDALREN
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCEECCCCEEEECCEEEEEECHHHHHHHHHC
ASKATAIGTTQRGIGPAYEDKVGRRAIRLMDLADIDTLPHKIERLLTHHNALRRGLGLAE
CCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FEAGAILKELTALAPKLLPYAETVWRLLDIKRREGKRILFEGAQGALLDVDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVAAQAATGTGMGPGSVGYVLGICKAYTTRVGQGPFPTELDNEIGRKIGERGREFGTN
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TGRPRRCGWFDAVLVRQTVRTCGIHGLALTKLDILDGFEQIEVCVGYRLDGKEIDHLPAG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCEEECCCCCCCCCCC
EGAQARVEPIYETIEGWKEPTANARSWAELPAQAIKYVRRIEELVGCPVALLSTSPERED
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILVQNPFEA
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA