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The map label for this gene is fusA [C]

Identifier: 86748425

GI number: 86748425

Start: 1481960

End: 1484026

Strand: Direct

Name: fusA [C]

Synonym: RPB_1300

Alternate gene names: 86748425

Gene position: 1481960-1484026 (Clockwise)

Preceding gene: 86748424

Following gene: 86748426

Centisome position: 27.8

GC content: 68.12

Gene sequence:

>2067_bases
ATGGGACAAGACCTCAGAAGTCCCCCAAATACTAAAGAATCGGGTCCCCGGTGCATTGCATTGGTGGGCCCATTCCAAAG
CGGAAAAACCACACTGCTCGAAGCGATCCTCGCGCGTACCGGCGCCGTGCCGCGCGCCGGCTCGGTCGATGCTGGTACCT
CGTATGGCGACTCCAGCCCGGAAGCGCGCCAGCACAAGATGGGGCTCGGCCTCACCGCGGCGACCACGCAGTTCATGGGC
GAGACCTACACCTTCCTCGATTGTCCGGGCTCGGTCGAATTCGCGCACGACATGCGCGCCGCGCTGCCCGCGGTCGATGC
CGCCGTCGTGGTGTGCGAGGCCGACGAGCGCAAGCTGCCGCAATTGCAGATCATCCTGCGCGAGCTCGAAGATCTCGGGA
TTCCGCGCTTCCTGTTTCTCAACAAGATCGATCGCGCCACCAAACGCATCCGCGAAGTGCTCGACAGCCTGCAGCCGGCC
TCGCGAATCCCGCTGGTGCTGCGCCAGATCCCGATCTGGAACGGCGACCTGATCGCTGGCTATGTCGATCTCGCGCTGGA
GCGCGCCTTCGTCTATCGCGAGCACAAACCCTCCGAAGTGATCGCGCTGGAAGGCGGCGATCTCGACCGCGAGAAGGAAG
CCCGCTTCTCGATGCTGGAGAAGCTCGCCGATCACGACGACGCGCTGATGGAGCAATTGCTCGAAGACATTCCGCCGCCG
CGCGACACGGTGTTCGACGATCTCGCGCGCGAACTGCGCGAAGGCGTGATCTGCCCGGTGCTGCTGGGCTCGGCGCTGCG
CGAGAACGGCGTGATGCGGCTGTTGAAGGCGCTGCGCCACGAAGCCCCCGACGTCGAAGAGACCGCGTCGCGGCTCGGCG
TCGCGGCGTCCAAGGACGCGGTCGGCTACGTGCTGAAGACCACCCATCTGCAGCACGGCGGCAAGCTGTCGCTGGCGCGG
GTGTTCGCCGGCACGTTGGCCGACGGCGACACCGTGCACGCACCGTCCGGCGAAGCCGCGCGGGTGTCGGGCATCCACAC
CGCGAATGGCGGCGCCGACAGCAAGCGCTCGTCGGCGGTGGCCGGTGAGGTGGTCGCACTCGGCAAGCTCGACGCGATCA
AGACCGGCGATACGTTCGGCAACGGCAAGACCGCGCCCTCGGCGCTGGTCGAGATCGCGCCCGCACCGCCGGTGCTGGCG
ATGGCGCTGGCCGCCGCCGACCGCAAGGACGACGTCAAGCTCGGCCAGTCGCTGCAGCGGCTTTCCGAGGAAGACCCGTC
GCTGACCGTGATTCACGATCCGCAGACCCACGACATCGTGCTGTGGGGGCAGGGCGAAATGCACCTGCGCGTCGCGCTGG
AGCGGCTCAAGGAGCGCTACGGCGTTTCGGTGAAGTCGCATCCACCGACGATCGGCTATCGCGAGACCATCCGCAAATCG
GCGACGCAGCGCGGCCGGCACAAGAAGCAGTCCGGCGGCCACGGCCAGTTCGGCGACGTGGTGCTGGAGGTGAAGCCGTT
GCCGCGTGGCGAGGGCTTCGTCTTCGCCGAGACGGTGGTCGGCGGCGCGGTGCCGCGCAACTACATCGGCGCGGTCGAAG
AAGGCGCGGTCGATGGCCTGCGCGCGGGGCCGCTCGGCTTCCCGGTGGTCGACGTCCACGTCACGCTGACCGACGGCTCG
TATCACAGCGTCGATTCCTCGGATCAGGCGTTCCGCACCGCGGCGCGGATCGGCATCACCGAGGCGCTGCCGCAGTGCCA
GCCGGTGCTGCTGGAGCCGATCCACAATGTCGAGATCGTCTGCCCGAACGACGCCACCGCCAAGGTCAACGCCATCCTGT
CGGGACGACGCGGCCAGATCCTCGGCTTCGATACCCGCGAGGGCTGGCCGGGCTGGGACGTGGTCCGCGCCACCCTGCCG
GAGGCGGAGATCGGCGATCTGATCGTCGAACTGCGCTCCGCCACCGCCGGCGCCGGCAGTTTCACCCGCAGCTTCGACCA
TATGGCCGAAGTCAACGGCCGCACCGCGGATCAGATCGTCGCCGCGCGCCGGCAGGTCGCGGCGTGA

Upstream 100 bases:

>100_bases
GCAAGCTTCGTAATTTTTCGCGCTTGCCAAGCGGCGAGGCTGCTGAAAGCATTGCCTCGCCGACCCAATCAAGGGCCGAG
CTCCAGACAAGGAGGCGGCG

Downstream 100 bases:

>100_bases
GCTGACCGGCGGGGTCGCAGACGCAGAGTCGAAAACGGCTTCAATGATGTTTCAAGCATCATTGAAGCCGTTTTATATTT
GCGCTAGCCGATAGGGCGGA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MGQDLRSPPNTKESGPRCIALVGPFQSGKTTLLEAILARTGAVPRAGSVDAGTSYGDSSPEARQHKMGLGLTAATTQFMG
ETYTFLDCPGSVEFAHDMRAALPAVDAAVVVCEADERKLPQLQIILRELEDLGIPRFLFLNKIDRATKRIREVLDSLQPA
SRIPLVLRQIPIWNGDLIAGYVDLALERAFVYREHKPSEVIALEGGDLDREKEARFSMLEKLADHDDALMEQLLEDIPPP
RDTVFDDLARELREGVICPVLLGSALRENGVMRLLKALRHEAPDVEETASRLGVAASKDAVGYVLKTTHLQHGGKLSLAR
VFAGTLADGDTVHAPSGEAARVSGIHTANGGADSKRSSAVAGEVVALGKLDAIKTGDTFGNGKTAPSALVEIAPAPPVLA
MALAAADRKDDVKLGQSLQRLSEEDPSLTVIHDPQTHDIVLWGQGEMHLRVALERLKERYGVSVKSHPPTIGYRETIRKS
ATQRGRHKKQSGGHGQFGDVVLEVKPLPRGEGFVFAETVVGGAVPRNYIGAVEEGAVDGLRAGPLGFPVVDVHVTLTDGS
YHSVDSSDQAFRTAARIGITEALPQCQPVLLEPIHNVEIVCPNDATAKVNAILSGRRGQILGFDTREGWPGWDVVRATLP
EAEIGDLIVELRSATAGAGSFTRSFDHMAEVNGRTADQIVAARRQVAA

Sequences:

>Translated_688_residues
MGQDLRSPPNTKESGPRCIALVGPFQSGKTTLLEAILARTGAVPRAGSVDAGTSYGDSSPEARQHKMGLGLTAATTQFMG
ETYTFLDCPGSVEFAHDMRAALPAVDAAVVVCEADERKLPQLQIILRELEDLGIPRFLFLNKIDRATKRIREVLDSLQPA
SRIPLVLRQIPIWNGDLIAGYVDLALERAFVYREHKPSEVIALEGGDLDREKEARFSMLEKLADHDDALMEQLLEDIPPP
RDTVFDDLARELREGVICPVLLGSALRENGVMRLLKALRHEAPDVEETASRLGVAASKDAVGYVLKTTHLQHGGKLSLAR
VFAGTLADGDTVHAPSGEAARVSGIHTANGGADSKRSSAVAGEVVALGKLDAIKTGDTFGNGKTAPSALVEIAPAPPVLA
MALAAADRKDDVKLGQSLQRLSEEDPSLTVIHDPQTHDIVLWGQGEMHLRVALERLKERYGVSVKSHPPTIGYRETIRKS
ATQRGRHKKQSGGHGQFGDVVLEVKPLPRGEGFVFAETVVGGAVPRNYIGAVEEGAVDGLRAGPLGFPVVDVHVTLTDGS
YHSVDSSDQAFRTAARIGITEALPQCQPVLLEPIHNVEIVCPNDATAKVNAILSGRRGQILGFDTREGWPGWDVVRATLP
EAEIGDLIVELRSATAGAGSFTRSFDHMAEVNGRTADQIVAARRQVAA
>Mature_687_residues
GQDLRSPPNTKESGPRCIALVGPFQSGKTTLLEAILARTGAVPRAGSVDAGTSYGDSSPEARQHKMGLGLTAATTQFMGE
TYTFLDCPGSVEFAHDMRAALPAVDAAVVVCEADERKLPQLQIILRELEDLGIPRFLFLNKIDRATKRIREVLDSLQPAS
RIPLVLRQIPIWNGDLIAGYVDLALERAFVYREHKPSEVIALEGGDLDREKEARFSMLEKLADHDDALMEQLLEDIPPPR
DTVFDDLARELREGVICPVLLGSALRENGVMRLLKALRHEAPDVEETASRLGVAASKDAVGYVLKTTHLQHGGKLSLARV
FAGTLADGDTVHAPSGEAARVSGIHTANGGADSKRSSAVAGEVVALGKLDAIKTGDTFGNGKTAPSALVEIAPAPPVLAM
ALAAADRKDDVKLGQSLQRLSEEDPSLTVIHDPQTHDIVLWGQGEMHLRVALERLKERYGVSVKSHPPTIGYRETIRKSA
TQRGRHKKQSGGHGQFGDVVLEVKPLPRGEGFVFAETVVGGAVPRNYIGAVEEGAVDGLRAGPLGFPVVDVHVTLTDGSY
HSVDSSDQAFRTAARIGITEALPQCQPVLLEPIHNVEIVCPNDATAKVNAILSGRRGQILGFDTREGWPGWDVVRATLPE
AEIGDLIVELRSATAGAGSFTRSFDHMAEVNGRTADQIVAARRQVAA

Specific function: This Protein Promotes The GTP-Dependent Translocation Of The Nascent Protein Chain From The A-Site To The P-Site Of The Ribosome. [C]

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=687, Percent_Identity=28.3842794759825, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI19923640, Length=740, Percent_Identity=26.3513513513513, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI25306287, Length=718, Percent_Identity=26.1838440111421, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI25306283, Length=407, Percent_Identity=27.027027027027, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1789738, Length=708, Percent_Identity=31.3559322033898, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790835, Length=473, Percent_Identity=25.369978858351, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=704, Percent_Identity=28.125, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17556745, Length=704, Percent_Identity=22.8693181818182, Blast_Score=127, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323098, Length=682, Percent_Identity=29.1788856304985, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322359, Length=686, Percent_Identity=23.6151603498542, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324707, Length=473, Percent_Identity=24.5243128964059, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=473, Percent_Identity=24.5243128964059, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24582462, Length=692, Percent_Identity=29.3352601156069, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI221458488, Length=725, Percent_Identity=24.8275862068966, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 73780; Mature: 73648

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQDLRSPPNTKESGPRCIALVGPFQSGKTTLLEAILARTGAVPRAGSVDAGTSYGDSSP
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EARQHKMGLGLTAATTQFMGETYTFLDCPGSVEFAHDMRAALPAVDAAVVVCEADERKLP
HHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCH
QLQIILRELEDLGIPRFLFLNKIDRATKRIREVLDSLQPASRIPLVLRQIPIWNGDLIAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCCHHHH
YVDLALERAFVYREHKPSEVIALEGGDLDREKEARFSMLEKLADHDDALMEQLLEDIPPP
HHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
RDTVFDDLARELREGVICPVLLGSALRENGVMRLLKALRHEAPDVEETASRLGVAASKDA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCH
VGYVLKTTHLQHGGKLSLARVFAGTLADGDTVHAPSGEAARVSGIHTANGGADSKRSSAV
HEEEEEEHHHCCCCCCHHHHHHHHHCCCCCEEECCCCCCEEEECEEECCCCCCCCHHHHH
AGEVVALGKLDAIKTGDTFGNGKTAPSALVEIAPAPPVLAMALAAADRKDDVKLGQSLQR
HHHEEEECCCCCEECCCCCCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
LSEEDPSLTVIHDPQTHDIVLWGQGEMHLRVALERLKERYGVSVKSHPPTIGYRETIRKS
HHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ATQRGRHKKQSGGHGQFGDVVLEVKPLPRGEGFVFAETVVGGAVPRNYIGAVEEGAVDGL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHCCCCCHHHHCCHHHCCCCCC
RAGPLGFPVVDVHVTLTDGSYHSVDSSDQAFRTAARIGITEALPQCQPVLLEPIHNVEIV
CCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEE
CPNDATAKVNAILSGRRGQILGFDTREGWPGWDVVRATLPEAEIGDLIVELRSATAGAGS
ECCCCCCEEHHEECCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
FTRSFDHMAEVNGRTADQIVAARRQVAA
HHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GQDLRSPPNTKESGPRCIALVGPFQSGKTTLLEAILARTGAVPRAGSVDAGTSYGDSSP
CCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EARQHKMGLGLTAATTQFMGETYTFLDCPGSVEFAHDMRAALPAVDAAVVVCEADERKLP
HHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCH
QLQIILRELEDLGIPRFLFLNKIDRATKRIREVLDSLQPASRIPLVLRQIPIWNGDLIAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCCHHHH
YVDLALERAFVYREHKPSEVIALEGGDLDREKEARFSMLEKLADHDDALMEQLLEDIPPP
HHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
RDTVFDDLARELREGVICPVLLGSALRENGVMRLLKALRHEAPDVEETASRLGVAASKDA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCH
VGYVLKTTHLQHGGKLSLARVFAGTLADGDTVHAPSGEAARVSGIHTANGGADSKRSSAV
HEEEEEEHHHCCCCCCHHHHHHHHHCCCCCEEECCCCCCEEEECEEECCCCCCCCHHHHH
AGEVVALGKLDAIKTGDTFGNGKTAPSALVEIAPAPPVLAMALAAADRKDDVKLGQSLQR
HHHEEEECCCCCEECCCCCCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
LSEEDPSLTVIHDPQTHDIVLWGQGEMHLRVALERLKERYGVSVKSHPPTIGYRETIRKS
HHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ATQRGRHKKQSGGHGQFGDVVLEVKPLPRGEGFVFAETVVGGAVPRNYIGAVEEGAVDGL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHCCCCCHHHHCCHHHCCCCCC
RAGPLGFPVVDVHVTLTDGSYHSVDSSDQAFRTAARIGITEALPQCQPVLLEPIHNVEIV
CCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEE
CPNDATAKVNAILSGRRGQILGFDTREGWPGWDVVRATLPEAEIGDLIVELRSATAGAGS
ECCCCCCEEHHEECCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
FTRSFDHMAEVNGRTADQIVAARRQVAA
HHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]