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The map label for this gene is livM [H]

Identifier: 86748379

GI number: 86748379

Start: 1435621

End: 1436772

Strand: Direct

Name: livM [H]

Synonym: RPB_1254

Alternate gene names: 86748379

Gene position: 1435621-1436772 (Clockwise)

Preceding gene: 86748378

Following gene: 86748380

Centisome position: 26.93

GC content: 64.24

Gene sequence:

>1152_bases
ATGGAAAGCCCGCGCTTTCTCAGCCGCTCCGACCTGATCGGTCTCGCCGCGCTCGCTCTGCTCCTGGTCGTGATCCTGCC
GCTGTCGCTGGATGTGTTCCGCCTCAACCTGGTGGCGAAGTATCTGACCTACGCCTTCGTGGCGCTCGGTCTGGTGCTGT
GCTGGGGCTTCGGCGGCATCTTGAGCCTCGGGCAGGGGGTGTTCTTCGGCCTCGGCGGCTACGGCATGGCGATGTATCTG
AAGCTGGAAGCCTCCAGCGTCGCCAACACCAAGATCCAGTCCACGCCCGGCATCCCGGATTTCATGGACTGGAACCAGAT
CACGCAATTGCCGCTGTTCTGGCAGCCGTTCCACAGCCTGACGCTGACGATCCTCGCGATCCTGATCGTGCCGACGCTGT
TCGCCTATCTGATCGGCGCGGCGATGTTCAAGCGGCGCGTCGGCGGCGTGTATTTCGCCATCATCACCCAGGCGATCGCC
GCGATCCTCACCATCCTGATCATCGGCCAGCAGGGCTACACCGGCGGCATCAACGGCATCACCGATCTGCGCACGCTGAA
GGGCTGGGACATCCGTCCCGACCACGCCAAGGTGGTGCTTTACTTCGTCGAGGTGGCGTTCCTGTTCGGCTGCATCCTGC
TGGCGCTGTTCGTGCGCCACTCCAAGCTCGGCCGCATCCTGGTGGCGATGCGCGAGAAGGAGGACCGGGTGCGGTTCTCC
GGCTACAGCGTCGCCAATTTCAAGATCTTCGCGTTCTGCCTGGCGGCGATGTTCGCGGCGATCGGCGGCGCGATGTTCAC
GCTCAATGTCGGGTTCATGTCGCCGTCCTTCGTCGGCATCGTGCCGTCGATCGAGATGGTGATTTACACCGCGGTCGGCG
GCCGGCTGTCGATCTTCGGCGCGATCTACGGGACGCTGCTGGTCAATTTCGCCAAGACCAGCCTGTCGGAATCCTTCCCG
CAGCTCTGGTTGTTCGGGCTCGGCGCGCTGTTCATCGCGGTGGTGCTGATCTTCCCGAACGGACTCGCCGGGATCTGGCG
CGACCACGTCCAGCCGCTGATCGACAAGGTCCTCAACAGGCGCAAATCCGGCTCGGATCACACCAGCGGCAAGACGCTCG
GCCCGGTCGCCGGCGGCGCTCCGGCGGAATAG

Upstream 100 bases:

>100_bases
CGATGGCCAAGGTGCTCACATTGCTCGCCATCGTCGCGATCCTGATGGTTCGCCCGCAAGGCCTGTTCGCCCTCAAGGTT
CGCAAATAGGAGTCATCGAC

Downstream 100 bases:

>100_bases
GGGAGGCAACCATGCTGATCGGCCATCAACAACCCGACTTCCTGCTGGCGGTGGAAGGGCTCACCGTGTCGTTCGACGGC
TTCAAGGCGGTCAACGACCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MESPRFLSRSDLIGLAALALLLVVILPLSLDVFRLNLVAKYLTYAFVALGLVLCWGFGGILSLGQGVFFGLGGYGMAMYL
KLEASSVANTKIQSTPGIPDFMDWNQITQLPLFWQPFHSLTLTILAILIVPTLFAYLIGAAMFKRRVGGVYFAIITQAIA
AILTILIIGQQGYTGGINGITDLRTLKGWDIRPDHAKVVLYFVEVAFLFGCILLALFVRHSKLGRILVAMREKEDRVRFS
GYSVANFKIFAFCLAAMFAAIGGAMFTLNVGFMSPSFVGIVPSIEMVIYTAVGGRLSIFGAIYGTLLVNFAKTSLSESFP
QLWLFGLGALFIAVVLIFPNGLAGIWRDHVQPLIDKVLNRRKSGSDHTSGKTLGPVAGGAPAE

Sequences:

>Translated_383_residues
MESPRFLSRSDLIGLAALALLLVVILPLSLDVFRLNLVAKYLTYAFVALGLVLCWGFGGILSLGQGVFFGLGGYGMAMYL
KLEASSVANTKIQSTPGIPDFMDWNQITQLPLFWQPFHSLTLTILAILIVPTLFAYLIGAAMFKRRVGGVYFAIITQAIA
AILTILIIGQQGYTGGINGITDLRTLKGWDIRPDHAKVVLYFVEVAFLFGCILLALFVRHSKLGRILVAMREKEDRVRFS
GYSVANFKIFAFCLAAMFAAIGGAMFTLNVGFMSPSFVGIVPSIEMVIYTAVGGRLSIFGAIYGTLLVNFAKTSLSESFP
QLWLFGLGALFIAVVLIFPNGLAGIWRDHVQPLIDKVLNRRKSGSDHTSGKTLGPVAGGAPAE
>Mature_383_residues
MESPRFLSRSDLIGLAALALLLVVILPLSLDVFRLNLVAKYLTYAFVALGLVLCWGFGGILSLGQGVFFGLGGYGMAMYL
KLEASSVANTKIQSTPGIPDFMDWNQITQLPLFWQPFHSLTLTILAILIVPTLFAYLIGAAMFKRRVGGVYFAIITQAIA
AILTILIIGQQGYTGGINGITDLRTLKGWDIRPDHAKVVLYFVEVAFLFGCILLALFVRHSKLGRILVAMREKEDRVRFS
GYSVANFKIFAFCLAAMFAAIGGAMFTLNVGFMSPSFVGIVPSIEMVIYTAVGGRLSIFGAIYGTLLVNFAKTSLSESFP
QLWLFGLGALFIAVVLIFPNGLAGIWRDHVQPLIDKVLNRRKSGSDHTSGKTLGPVAGGAPAE

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=332, Percent_Identity=26.2048192771084, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41519; Mature: 41519

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESPRFLSRSDLIGLAALALLLVVILPLSLDVFRLNLVAKYLTYAFVALGLVLCWGFGGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLGQGVFFGLGGYGMAMYLKLEASSVANTKIQSTPGIPDFMDWNQITQLPLFWQPFHSL
HHHCCHHHEECCCCCEEEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
TLTILAILIVPTLFAYLIGAAMFKRRVGGVYFAIITQAIAAILTILIIGQQGYTGGINGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
TDLRTLKGWDIRPDHAKVVLYFVEVAFLFGCILLALFVRHSKLGRILVAMREKEDRVRFS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
GYSVANFKIFAFCLAAMFAAIGGAMFTLNVGFMSPSFVGIVPSIEMVIYTAVGGRLSIFG
CCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCHHHHHEEECCCCHHHHHH
AIYGTLLVNFAKTSLSESFPQLWLFGLGALFIAVVLIFPNGLAGIWRDHVQPLIDKVLNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKSGSDHTSGKTLGPVAGGAPAE
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MESPRFLSRSDLIGLAALALLLVVILPLSLDVFRLNLVAKYLTYAFVALGLVLCWGFGGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLGQGVFFGLGGYGMAMYLKLEASSVANTKIQSTPGIPDFMDWNQITQLPLFWQPFHSL
HHHCCHHHEECCCCCEEEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
TLTILAILIVPTLFAYLIGAAMFKRRVGGVYFAIITQAIAAILTILIIGQQGYTGGINGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
TDLRTLKGWDIRPDHAKVVLYFVEVAFLFGCILLALFVRHSKLGRILVAMREKEDRVRFS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
GYSVANFKIFAFCLAAMFAAIGGAMFTLNVGFMSPSFVGIVPSIEMVIYTAVGGRLSIFG
CCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCHHHHHEEECCCCHHHHHH
AIYGTLLVNFAKTSLSESFPQLWLFGLGALFIAVVLIFPNGLAGIWRDHVQPLIDKVLNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKSGSDHTSGKTLGPVAGGAPAE
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]