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The map label for this gene is 86748341

Identifier: 86748341

GI number: 86748341

Start: 1390031

End: 1391434

Strand: Reverse

Name: 86748341

Synonym: RPB_1216

Alternate gene names: NA

Gene position: 1391434-1390031 (Counterclockwise)

Preceding gene: 86748342

Following gene: 86748339

Centisome position: 26.1

GC content: 67.59

Gene sequence:

>1404_bases
ATGTTGCAGGTCACCGGAGTTCCGGATGCGTGCGATAATCTGGCGCCGATCCCGATGGAGTGCGACGCCGCGTTTCTGTC
GATCAAGGGCGAGCTGCCGCGCGAATTGAACGGCACGCTGTATCGCAACGGCGCCAATCCGCAATTCGCATCAAAGAACG
CGCATTGGTTCTTCGGCGACGGCATGCTGCATGCCTTCCGGCTGGAGAACGGCCGCGCCAGCTATCGCAACCGCTGGGTC
CGCACCCCGAAATGGCTGGCCGAGCACGAAGCCGGCCGGCCGCTTTACGGCGAGTTCAATCTCAAGCGGCCCGATGCGCC
GCGTTCGGCGCCCGACGACGGCAACGTCGCCAACACCAACATCGTGTTCCACGCCGGCCGGCTGCTGGCGCTGGAAGAGG
CGCATCTGCCGATCGAAATCGAGCGCGACACGCTGGCGACGCGCGGCTATTGCGACTATGGCGGGGCGCTGAAAGGGCCG
TTCACCGCGCATCCGAAGATCGACCCGGTCACCGGCGAGATGCTGTTCTTCGGCTACAATGCCGACGGCCCGTTGAAACG
GACGATGTCGTTCGGCGCGATCGACGCGTCCGGCCACGTCACCCGGTTCGAGCGCTTCAAGGCGCCCTATGCAGCGATGG
TGCACGATTTCATCGTCACCGAGAACTATGTGCTGTTTCCGATCCTGCCGCTCACCGGCAGCATCTGGCGGGCGATGCGC
GGCCGCCCGCCTTATGCCTGGGACCCCGCTAAGGGCTCTTACGTCGGCGTGATGAAGCGCACCGGCTCGACCCGCGACAT
CCGCTGGTTCCGCGGTGACGCCTGCTTCGTGTTCCACGTCATGAACGCGTGGGAGGACGGCACCAAGATCGTCGCCGACG
TGATGCAATCCGAGGAAGCGCCGCTGTTCACCCATCCGGACGGCCGCCGCACCGATCCGGAGAAGGGCCGCGCCCGGCTG
TGCCGCTGGAGCTTCGACCTCGCCGGCAACACCAACGCCTTCACGCGCAGCTATCTCGACGACATCAGCGGCGAATTCCC
GCGGATCGACGAGCGCCGCGCCGGCCTGCGCAGCGGCCATGGCTGGTACGCCTGCGCCAGCCCGGAGACGCCGACGCTCG
GGATGCTGACGGGGCTGGTGCATGTCGACGGCAACGGCGATCGCCGCGCCCGCTATCTGCTGCCGACCGGCGACAGCATC
GGCGAGCCGGTGTTCGTGCCGCGCACGCCGGATGCGGCGGAAGCCGATGGCTGGATCCTCACGGTGATCTGGCGCGGCTG
CGAGAACCGCAGCGACCTCGCGGTGTTCGACGCCGCCGACATCGCGGGCGGCCCGATCGCGCTGGTCCAACTCGGCCACC
GCGTCCCGGACGGCTTCCACGGCAATTGGGTGGCGGCGGGGTGA

Upstream 100 bases:

>100_bases
GCGGTTCCGGAGAACTACTCACATGCGGTGCAGCCGTTCGGCCGGCTGCTGACGTTGAACTAATCGCGGCCGCCGCGACG
CCGACGACAGGAGCACGACC

Downstream 100 bases:

>100_bases
CCAACGACACCCGTCCAATTTTGACTCCGTCATTCCGGGGCGCGAGCGAAGCTCGCGAACCCGGAATCTCGAGGTTCAGG
GCATTCCGAGCACGCCGCGC

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLQVTGVPDACDNLAPIPMECDAAFLSIKGELPRELNGTLYRNGANPQFASKNAHWFFGDGMLHAFRLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKRPDAPRSAPDDGNVANTNIVFHAGRLLALEEAHLPIEIERDTLATRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNADGPLKRTMSFGAIDASGHVTRFERFKAPYAAMVHDFIVTENYVLFPILPLTGSIWRAMR
GRPPYAWDPAKGSYVGVMKRTGSTRDIRWFRGDACFVFHVMNAWEDGTKIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFTRSYLDDISGEFPRIDERRAGLRSGHGWYACASPETPTLGMLTGLVHVDGNGDRRARYLLPTGDSI
GEPVFVPRTPDAAEADGWILTVIWRGCENRSDLAVFDAADIAGGPIALVQLGHRVPDGFHGNWVAAG

Sequences:

>Translated_467_residues
MLQVTGVPDACDNLAPIPMECDAAFLSIKGELPRELNGTLYRNGANPQFASKNAHWFFGDGMLHAFRLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKRPDAPRSAPDDGNVANTNIVFHAGRLLALEEAHLPIEIERDTLATRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNADGPLKRTMSFGAIDASGHVTRFERFKAPYAAMVHDFIVTENYVLFPILPLTGSIWRAMR
GRPPYAWDPAKGSYVGVMKRTGSTRDIRWFRGDACFVFHVMNAWEDGTKIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFTRSYLDDISGEFPRIDERRAGLRSGHGWYACASPETPTLGMLTGLVHVDGNGDRRARYLLPTGDSI
GEPVFVPRTPDAAEADGWILTVIWRGCENRSDLAVFDAADIAGGPIALVQLGHRVPDGFHGNWVAAG
>Mature_467_residues
MLQVTGVPDACDNLAPIPMECDAAFLSIKGELPRELNGTLYRNGANPQFASKNAHWFFGDGMLHAFRLENGRASYRNRWV
RTPKWLAEHEAGRPLYGEFNLKRPDAPRSAPDDGNVANTNIVFHAGRLLALEEAHLPIEIERDTLATRGYCDYGGALKGP
FTAHPKIDPVTGEMLFFGYNADGPLKRTMSFGAIDASGHVTRFERFKAPYAAMVHDFIVTENYVLFPILPLTGSIWRAMR
GRPPYAWDPAKGSYVGVMKRTGSTRDIRWFRGDACFVFHVMNAWEDGTKIVADVMQSEEAPLFTHPDGRRTDPEKGRARL
CRWSFDLAGNTNAFTRSYLDDISGEFPRIDERRAGLRSGHGWYACASPETPTLGMLTGLVHVDGNGDRRARYLLPTGDSI
GEPVFVPRTPDAAEADGWILTVIWRGCENRSDLAVFDAADIAGGPIALVQLGHRVPDGFHGNWVAAG

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=521, Percent_Identity=23.0326295585413, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI82617624, Length=521, Percent_Identity=23.0326295585413, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 51709; Mature: 51709

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVTGVPDACDNLAPIPMECDAAFLSIKGELPRELNGTLYRNGANPQFASKNAHWFFGD
CEEECCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECC
GMLHAFRLENGRASYRNRWVRTPKWLAEHEAGRPLYGEFNLKRPDAPRSAPDDGNVANTN
CEEEEEEECCCCHHHHHCCCCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEECCE
IVFHAGRLLALEEAHLPIEIERDTLATRGYCDYGGALKGPFTAHPKIDPVTGEMLFFGYN
EEEECCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
ADGPLKRTMSFGAIDASGHVTRFERFKAPYAAMVHDFIVTENYVLFPILPLTGSIWRAMR
CCCCHHHHEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHC
GRPPYAWDPAKGSYVGVMKRTGSTRDIRWFRGDACFVFHVMNAWEDGTKIVADVMQSEEA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCEEEEEEEHHHCCCCHHHHHHHHHCCCC
PLFTHPDGRRTDPEKGRARLCRWSFDLAGNTNAFTRSYLDDISGEFPRIDERRAGLRSGH
CEEECCCCCCCCCHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCC
GWYACASPETPTLGMLTGLVHVDGNGDRRARYLLPTGDSIGEPVFVPRTPDAAEADGWIL
CEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCEEE
TVIWRGCENRSDLAVFDAADIAGGPIALVQLGHRVPDGFHGNWVAAG
EEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEECC
>Mature Secondary Structure
MLQVTGVPDACDNLAPIPMECDAAFLSIKGELPRELNGTLYRNGANPQFASKNAHWFFGD
CEEECCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECC
GMLHAFRLENGRASYRNRWVRTPKWLAEHEAGRPLYGEFNLKRPDAPRSAPDDGNVANTN
CEEEEEEECCCCHHHHHCCCCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEECCE
IVFHAGRLLALEEAHLPIEIERDTLATRGYCDYGGALKGPFTAHPKIDPVTGEMLFFGYN
EEEECCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
ADGPLKRTMSFGAIDASGHVTRFERFKAPYAAMVHDFIVTENYVLFPILPLTGSIWRAMR
CCCCHHHHEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHC
GRPPYAWDPAKGSYVGVMKRTGSTRDIRWFRGDACFVFHVMNAWEDGTKIVADVMQSEEA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCEEEEEEEHHHCCCCHHHHHHHHHCCCC
PLFTHPDGRRTDPEKGRARLCRWSFDLAGNTNAFTRSYLDDISGEFPRIDERRAGLRSGH
CEEECCCCCCCCCHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCC
GWYACASPETPTLGMLTGLVHVDGNGDRRARYLLPTGDSIGEPVFVPRTPDAAEADGWIL
CEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCEEE
TVIWRGCENRSDLAVFDAADIAGGPIALVQLGHRVPDGFHGNWVAAG
EEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]