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The map label for this gene is hisS [H]

Identifier: 86748310

GI number: 86748310

Start: 1359230

End: 1360810

Strand: Direct

Name: hisS [H]

Synonym: RPB_1185

Alternate gene names: 86748310

Gene position: 1359230-1360810 (Clockwise)

Preceding gene: 86748306

Following gene: 86748311

Centisome position: 25.49

GC content: 63.31

Gene sequence:

>1581_bases
ATGGCTGAGAAACCCAAGAAACCACAGAAGTTGCGCGCCCGTCTGCCGCGCGGCCTGACCGATCGCGGCCCCGCCGAGAT
CGCGGCGACGCGCGCGATGGTGGAAACCATCCGCGAGGTCTATGAGCGCTACGGCTTCGAGCCGGTCGAGACCCCGGCGT
TCGAATACACCGACGCGCTCGGCAAGTTCCTGCCCGACCAGGATCGCCCCAACGAGGGCGTGTTCTCGCTGCAGGACGAC
GACGAGCAATGGATCAGCCTGCGCTACGACCTGACCGCGCCGCTCGCCCGCTACGTCGCCGAAAATTTCGACGCACTGCC
GAAGCCCTATCGCAGCTACCGTTTCGGCTGGGTGTTCCGCAACGAAAAGCCCGGCCCCGGCCGCTTCCGCCAGTTCATGC
AGTTCGACGCCGACACCGTGGGCTCCGGCTCGCCGGCCGCCGATGCCGAGATGTGCATGATGGCGGCGGACACGATGGAA
GCGTTGGGCATCCCGCGCGGCAGCTACGTGGTGAAGGTGAACAACCGCAAGGTGCTGGATGGGGTGCTGGAGGCCATTGG
CCTTGGCGGGGATGAGAATGCGGGGCGCCGGCTCACGGTGCTGAGGGCTATCGATAAGTCGGATAAATTTCCGCCCGAGG
AGATCAAGAAGCTTCTGGGGCCGGGACGTTGGGATGGTGGCGAAGAAGGCAAAGGCGATTTCACAAAAGGCGCGATGCTT
GGTGATGATCAGATTGAGCTGATTCTCAGAGCAACTTCGCCAAGCTTCATAGCAGGCCGCTTCAATGCCGATGGAAGCGG
CGGCGTTAGCAATGCTGATACAGTCGAACTCCTTCGCTCGACGGCGGACAACGAGACTTTAAAGCAAGGATGCGATGAAC
TGTCGGTAATCTCGGATCTGCTGGATTCTGGCGGCTATGGTGCAACGTCCACAAATCCTAATGTGCGCGTTGTCATCGAT
CCCTCCGTCGTCCGAGGCCTCGAATACTACACCGGCCCGGTCTACGAGGTCGAACTGCTGCTCGAGACCAAGGACGAAAA
GGGGCGCCCGGTGCGGTTCGGCTCGGTCGGCGGCGGCGGTCGTTACGATGGTCTGGTGTCGCGCTTCCGCGGCGAGCCGG
TGCCGGCGACCGGGTTCTCGATCGGTGTGTCGCGGCTGCAGGCGGCGCTGACGATGATCGGCAAGCTTGGGACCCGGCCC
GCGACCGGCCCGGTGGTGGTGACGGTGTTCGACCGCGAGCGGCTCGCCGACTACCAGAAGATGGTGTCGCAGCTCCGCGC
CGAGGACATCCGCGCCGAGCTCTATCTCGGCAATCCGAAGAACATGGGCAACCAGCTCAAATACGCCGACAAGCGCAACT
CGCCTTGCGTGATCATCCAGGGCTCCGATGAGAAGAACGATCCGGACGGCCCGCAGGTGATCGTCAAGGACCTGATCCTC
GGCGCCGAACTCGCCGCTCTGGACAAGGATCGCGACGATTATCTGCAGCGTCAGGCCGACGCCCAGCGCAAAGTGCCGCA
GCTCGGGATGATCGACGAAGTGCGGCGGATTCTGGCGCGGCACGACATCGACTGGAATTGA

Upstream 100 bases:

>100_bases
CGGAGGCAGCCGGCCCGCGATTTGCGCTGGATCATGCAGCCCAGCCTTGCTGCACGGCAGGGCGGCTGGTACCAACCCGG
CCCTGAGCGGAAAGGCGACC

Downstream 100 bases:

>100_bases
CGGCGGACGGCGCGCTGTCGAACCCTCTCCCCTTGTGGGAGAGGGTCGCTTCGCGAAGCGAAGCGGGGTGAGGGGTCGAG
GGCCTTTACCGAGATCACAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MAEKPKKPQKLRARLPRGLTDRGPAEIAATRAMVETIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDD
DEQWISLRYDLTAPLARYVAENFDALPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIPRGSYVVKVNNRKVLDGVLEAIGLGGDENAGRRLTVLRAIDKSDKFPPEEIKKLLGPGRWDGGEEGKGDFTKGAML
GDDQIELILRATSPSFIAGRFNADGSGGVSNADTVELLRSTADNETLKQGCDELSVISDLLDSGGYGATSTNPNVRVVID
PSVVRGLEYYTGPVYEVELLLETKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTMIGKLGTRP
ATGPVVVTVFDRERLADYQKMVSQLRAEDIRAELYLGNPKNMGNQLKYADKRNSPCVIIQGSDEKNDPDGPQVIVKDLIL
GAELAALDKDRDDYLQRQADAQRKVPQLGMIDEVRRILARHDIDWN

Sequences:

>Translated_526_residues
MAEKPKKPQKLRARLPRGLTDRGPAEIAATRAMVETIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDD
DEQWISLRYDLTAPLARYVAENFDALPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIPRGSYVVKVNNRKVLDGVLEAIGLGGDENAGRRLTVLRAIDKSDKFPPEEIKKLLGPGRWDGGEEGKGDFTKGAML
GDDQIELILRATSPSFIAGRFNADGSGGVSNADTVELLRSTADNETLKQGCDELSVISDLLDSGGYGATSTNPNVRVVID
PSVVRGLEYYTGPVYEVELLLETKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTMIGKLGTRP
ATGPVVVTVFDRERLADYQKMVSQLRAEDIRAELYLGNPKNMGNQLKYADKRNSPCVIIQGSDEKNDPDGPQVIVKDLIL
GAELAALDKDRDDYLQRQADAQRKVPQLGMIDEVRRILARHDIDWN
>Mature_525_residues
AEKPKKPQKLRARLPRGLTDRGPAEIAATRAMVETIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDDD
EQWISLRYDLTAPLARYVAENFDALPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEA
LGIPRGSYVVKVNNRKVLDGVLEAIGLGGDENAGRRLTVLRAIDKSDKFPPEEIKKLLGPGRWDGGEEGKGDFTKGAMLG
DDQIELILRATSPSFIAGRFNADGSGGVSNADTVELLRSTADNETLKQGCDELSVISDLLDSGGYGATSTNPNVRVVIDP
SVVRGLEYYTGPVYEVELLLETKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTMIGKLGTRPA
TGPVVVTVFDRERLADYQKMVSQLRAEDIRAELYLGNPKNMGNQLKYADKRNSPCVIIQGSDEKNDPDGPQVIVKDLILG
AELAALDKDRDDYLQRQADAQRKVPQLGMIDEVRRILARHDIDWN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=472, Percent_Identity=31.5677966101695, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI15029520, Length=464, Percent_Identity=29.3103448275862, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788861, Length=215, Percent_Identity=28.3720930232558, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993686, Length=481, Percent_Identity=30.3534303534304, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71993693, Length=481, Percent_Identity=30.3534303534304, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325290, Length=496, Percent_Identity=28.0241935483871, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643061, Length=479, Percent_Identity=30.6889352818372, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24643059, Length=498, Percent_Identity=29.3172690763052, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 57964; Mature: 57833

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKPKKPQKLRARLPRGLTDRGPAEIAATRAMVETIREVYERYGFEPVETPAFEYTDAL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSLQDDDEQWISLRYDLTAPLARYVAENFDALPKPYRSYRFGWVFR
HHHCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHCCEEEEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIPRGSYVVKVNNRKVLD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGLGGDENAGRRLTVLRAIDKSDKFPPEEIKKLLGPGRWDGGEEGKGDFTKGAML
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GDDQIELILRATSPSFIAGRFNADGSGGVSNADTVELLRSTADNETLKQGCDELSVISDL
CCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDSGGYGATSTNPNVRVVIDPSVVRGLEYYTGPVYEVELLLETKDEKGRPVRFGSVGGGG
HHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCC
RYDGLVSRFRGEPVPATGFSIGVSRLQAALTMIGKLGTRPATGPVVVTVFDRERLADYQK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHH
MVSQLRAEDIRAELYLGNPKNMGNQLKYADKRNSPCVIIQGSDEKNDPDGPQVIVKDLIL
HHHHHHHHHCEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
GAELAALDKDRDDYLQRQADAQRKVPQLGMIDEVRRILARHDIDWN
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEKPKKPQKLRARLPRGLTDRGPAEIAATRAMVETIREVYERYGFEPVETPAFEYTDAL
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSLQDDDEQWISLRYDLTAPLARYVAENFDALPKPYRSYRFGWVFR
HHHCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHCCEEEEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIPRGSYVVKVNNRKVLD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGLGGDENAGRRLTVLRAIDKSDKFPPEEIKKLLGPGRWDGGEEGKGDFTKGAML
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GDDQIELILRATSPSFIAGRFNADGSGGVSNADTVELLRSTADNETLKQGCDELSVISDL
CCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDSGGYGATSTNPNVRVVIDPSVVRGLEYYTGPVYEVELLLETKDEKGRPVRFGSVGGGG
HHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCC
RYDGLVSRFRGEPVPATGFSIGVSRLQAALTMIGKLGTRPATGPVVVTVFDRERLADYQK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHH
MVSQLRAEDIRAELYLGNPKNMGNQLKYADKRNSPCVIIQGSDEKNDPDGPQVIVKDLIL
HHHHHHHHHCEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
GAELAALDKDRDDYLQRQADAQRKVPQLGMIDEVRRILARHDIDWN
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA