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The map label for this gene is rsmD [H]

Identifier: 86748297

GI number: 86748297

Start: 1346293

End: 1346856

Strand: Direct

Name: rsmD [H]

Synonym: RPB_1172

Alternate gene names: 86748297

Gene position: 1346293-1346856 (Clockwise)

Preceding gene: 86748296

Following gene: 86748303

Centisome position: 25.25

GC content: 68.44

Gene sequence:

>564_bases
ATGCGCGTGATCGGCGGGCGGCTGCGGGGCCGCAATCTGGTGGCGCCGTCGTCGCGCGACATCCGCCCGACGGCGGATCG
GCTGCGCGAGTCGCTGTTCAATATCCTGATGCACGCCTACGGCGACCCGATCGGTGACGCGCGCGTGCTCGATCTGTTCG
CCGGCACCGGCGCGCTCGGCATCGAGGCGGCGTCGCGCGGCGCCAAGTTCACGCTGTTCGTCGACAATGGCGCGGAAGCT
CGCGCGCTGCTCCGCGCCAATGTCGAGGCGCTCGGCCTCGGCGGCGTCAGCAAAGTGTATCGCCGCGACGCCACCAATCT
CGGCCCGGCGCATCCGGTCGAGCCGTTTTCACTGGTGTTCCTCGATCCGCCCTATCGCATGGGATTCGCCGAGAAAGCAT
TGGCCTCGCTGCGCGACGGCGGCTGGCTCACGCCCGACGCGCTGGTGATCGTCGAGGAAGCCAAGGACGCCGGCTTCGCC
GCGCCGGACGGTTTCGTCGAGCTGGAGCGGCGCGCTTATGACGATACGGAATTTGCGATGTTGCGGTTCGGAACGAGCGA
GTAG

Upstream 100 bases:

>100_bases
GGTCGAGCGCACCGGCAGCGGCGGCGACCGGGGCCTCACCCGCGGCCCCGCCCGGCGCTATCACGGCAAGCGCGAACCGG
CACCTCGCGAGGACTAACCG

Downstream 100 bases:

>100_bases
TAGCCTTTCTGGCTAGTAGCAGCTCGTTGTGGGGGACCTCCTATCAACGTCATTCCGGGGCGCGCGCAGCGCGAACCCGG
AATGACGGCGAGAGATGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MRVIGGRLRGRNLVAPSSRDIRPTADRLRESLFNILMHAYGDPIGDARVLDLFAGTGALGIEAASRGAKFTLFVDNGAEA
RALLRANVEALGLGGVSKVYRRDATNLGPAHPVEPFSLVFLDPPYRMGFAEKALASLRDGGWLTPDALVIVEEAKDAGFA
APDGFVELERRAYDDTEFAMLRFGTSE

Sequences:

>Translated_187_residues
MRVIGGRLRGRNLVAPSSRDIRPTADRLRESLFNILMHAYGDPIGDARVLDLFAGTGALGIEAASRGAKFTLFVDNGAEA
RALLRANVEALGLGGVSKVYRRDATNLGPAHPVEPFSLVFLDPPYRMGFAEKALASLRDGGWLTPDALVIVEEAKDAGFA
APDGFVELERRAYDDTEFAMLRFGTSE
>Mature_187_residues
MRVIGGRLRGRNLVAPSSRDIRPTADRLRESLFNILMHAYGDPIGDARVLDLFAGTGALGIEAASRGAKFTLFVDNGAEA
RALLRANVEALGLGGVSKVYRRDATNLGPAHPVEPFSLVFLDPPYRMGFAEKALASLRDGGWLTPDALVIVEEAKDAGFA
APDGFVELERRAYDDTEFAMLRFGTSE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=159, Percent_Identity=40.251572327044, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20209; Mature: 20209

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIGGRLRGRNLVAPSSRDIRPTADRLRESLFNILMHAYGDPIGDARVLDLFAGTGALG
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCC
IEAASRGAKFTLFVDNGAEARALLRANVEALGLGGVSKVYRRDATNLGPAHPVEPFSLVF
CCCCCCCCEEEEEEECCCHHHHHHHHCCCEECCCCHHHHHHHCCCCCCCCCCCCCEEEEE
LDPPYRMGFAEKALASLRDGGWLTPDALVIVEEAKDAGFAAPDGFVELERRAYDDTEFAM
ECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEE
LRFGTSE
EEECCCC
>Mature Secondary Structure
MRVIGGRLRGRNLVAPSSRDIRPTADRLRESLFNILMHAYGDPIGDARVLDLFAGTGALG
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCC
IEAASRGAKFTLFVDNGAEARALLRANVEALGLGGVSKVYRRDATNLGPAHPVEPFSLVF
CCCCCCCCEEEEEEECCCHHHHHHHHCCCEECCCCHHHHHHHCCCCCCCCCCCCCEEEEE
LDPPYRMGFAEKALASLRDGGWLTPDALVIVEEAKDAGFAAPDGFVELERRAYDDTEFAM
ECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEE
LRFGTSE
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]