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The map label for this gene is lcfB [H]

Identifier: 86748278

GI number: 86748278

Start: 1324796

End: 1326403

Strand: Direct

Name: lcfB [H]

Synonym: RPB_1153

Alternate gene names: 86748278

Gene position: 1324796-1326403 (Clockwise)

Preceding gene: 86748277

Following gene: 86748279

Centisome position: 24.85

GC content: 68.72

Gene sequence:

>1608_bases
ATGACGAACTCTGAGTACACGGACTGGACCGAGACCTCCGTAGCCGTTTCAGTCCAATTACGATCACGTCCCCTCTGGAG
TGCCTTCATGTCCTGGCCGTCCCGCAATCTCGGCTGCTGTTTCGATCCGTCGGCGCAACCCGACAAGGTCGCGATCATCG
ATCTGCGCCGCCCGGAGCAGCCGCAGGAGATCACCTACGCCGCGCTCGACGCCGCCTGCGACGCGGTGGCGCGCGGGCTG
CTCGGCCGCGGCCTGACGCGCGGCGACCGCGTCGGCATCCTGTCGCTGAATTCGGCGCCGATGATCGCGGCCTATCTGGG
CATCATGCGGGCCGGCCTCGTCGCGGTGCCGATCTCGTTCAAGCTGGCGCGCGACACCGTCGATTACATCGTCAAGGACG
CCGATCTGCGCGCCGTGTTCCACGATCACGAGCGTGCCGCGACGGTGCCGGACGGCGTGCTCGGCATCGATTTCGACGCG
CCGGACGGCTACGCGGCGCTGCTCGACCATGGCCCGTTCACCGCGATCGAGCCGCAGGCCCGCGAGGTCGCCACCATTCT
CTACACCTCGGGGTCGACCGGCATGCCGAAAGGCGTGCTGCTGTCGCACGAATCGCAGATGTGGGCGCTCGACACCGCGG
CGCGCGCCGGCGACCGCTCGCAGCATCGCTATATCGTCGCCGCGCCGATGTTCCACATGAACGCGACGATCAGCGCCAAG
ACCGCGCTCCATGCGGGCGCCTCGATGGTGCTGCTGCCGTCGTTCGACGCGCGGCTCTATGCGCAGGCGATCGCCAAATA
TCGCGTCACCTGGCTGACCTCGGTGCCGACCATGCTGGCGATGATGGCGCGCGAGCGCGACCTGATCGCCACGCTCGATT
TCTCCTCGGTCAGCCATGTGATGATGGGCTCGGCGCCGCTCACCAAGGCGCTGGTCGAGAAGGTGCAGGGCATCTTCCCG
GGGGCGGCGATCAGCAACGCCTATGGCACCACCGAGGCCGGCCCCGGCGTGTTCGGTCCGCATCCGGACGGCCTGCCGCG
GCCGGACACGGCGGTCGGCTATCCGACCGAGGGCGCGCTCGCCGAATTGCGCGAGGGCCCCTCGCCGGACGAGGGCGTGC
TCTACATGAAGAACCCGATGCTGATGGAGGGCTACAACAACCGGCCGGAGAAGACCGCCGAGGTGATGCGCGACGGCTGG
TATCGCTCCGGCGACATCATGCGCCGCGACGCGCACGGCTTCTTCCATTTCCTCGGGCGCGCCGACGACATGTTCGTGGT
CGGGGGCGAGAACGTCTGGCCCGGCGAGGTCGAGAAACTGATCGAGCGGATGCCCGGCGTGCACCAGGCCGCGGTGGTGC
CGGTGCCGGACGACATCAAGGGCACGCTGCCGTTCGCCTTCGTGGTGAAGCAGGACGGCGCCGCGATCGACGAGGCGGCG
GTGAAGACCTTCACCATCGCCAACGGCCCGGCCTTCGCCCACCCGCGCTTCGTCGAATTCCGCGCCGCCATCGCGTTATC
GGCGACCAACAAGCCGGACCGGCGGCTCTTGACCGAGGAAGCCCAGCAGATCGCCGCAAAGCGCCGCGCGGCCGGCGAGC
GGGGGTGA

Upstream 100 bases:

>100_bases
CCCGGATTCCTCAACCCGTCATGGCCGGGCTTGACCCGGCCATCCACGTCTTGCCACGTCGGCGAGCTCGAAGGCGTGGA
TGCCCGGGACGAGCCCGGGC

Downstream 100 bases:

>100_bases
GCCCCAACCGGGCAATGTCAGTGGTTAATGCACAGTTGAGGCGCAGGGGGAGGCGGTCGTCCGGAGGTATTTAGGTATAT
TTTTCGTTCGTTTTGACTGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTNSEYTDWTETSVAVSVQLRSRPLWSAFMSWPSRNLGCCFDPSAQPDKVAIIDLRRPEQPQEITYAALDAACDAVARGL
LGRGLTRGDRVGILSLNSAPMIAAYLGIMRAGLVAVPISFKLARDTVDYIVKDADLRAVFHDHERAATVPDGVLGIDFDA
PDGYAALLDHGPFTAIEPQAREVATILYTSGSTGMPKGVLLSHESQMWALDTAARAGDRSQHRYIVAAPMFHMNATISAK
TALHAGASMVLLPSFDARLYAQAIAKYRVTWLTSVPTMLAMMARERDLIATLDFSSVSHVMMGSAPLTKALVEKVQGIFP
GAAISNAYGTTEAGPGVFGPHPDGLPRPDTAVGYPTEGALAELREGPSPDEGVLYMKNPMLMEGYNNRPEKTAEVMRDGW
YRSGDIMRRDAHGFFHFLGRADDMFVVGGENVWPGEVEKLIERMPGVHQAAVVPVPDDIKGTLPFAFVVKQDGAAIDEAA
VKTFTIANGPAFAHPRFVEFRAAIALSATNKPDRRLLTEEAQQIAAKRRAAGERG

Sequences:

>Translated_535_residues
MTNSEYTDWTETSVAVSVQLRSRPLWSAFMSWPSRNLGCCFDPSAQPDKVAIIDLRRPEQPQEITYAALDAACDAVARGL
LGRGLTRGDRVGILSLNSAPMIAAYLGIMRAGLVAVPISFKLARDTVDYIVKDADLRAVFHDHERAATVPDGVLGIDFDA
PDGYAALLDHGPFTAIEPQAREVATILYTSGSTGMPKGVLLSHESQMWALDTAARAGDRSQHRYIVAAPMFHMNATISAK
TALHAGASMVLLPSFDARLYAQAIAKYRVTWLTSVPTMLAMMARERDLIATLDFSSVSHVMMGSAPLTKALVEKVQGIFP
GAAISNAYGTTEAGPGVFGPHPDGLPRPDTAVGYPTEGALAELREGPSPDEGVLYMKNPMLMEGYNNRPEKTAEVMRDGW
YRSGDIMRRDAHGFFHFLGRADDMFVVGGENVWPGEVEKLIERMPGVHQAAVVPVPDDIKGTLPFAFVVKQDGAAIDEAA
VKTFTIANGPAFAHPRFVEFRAAIALSATNKPDRRLLTEEAQQIAAKRRAAGERG
>Mature_534_residues
TNSEYTDWTETSVAVSVQLRSRPLWSAFMSWPSRNLGCCFDPSAQPDKVAIIDLRRPEQPQEITYAALDAACDAVARGLL
GRGLTRGDRVGILSLNSAPMIAAYLGIMRAGLVAVPISFKLARDTVDYIVKDADLRAVFHDHERAATVPDGVLGIDFDAP
DGYAALLDHGPFTAIEPQAREVATILYTSGSTGMPKGVLLSHESQMWALDTAARAGDRSQHRYIVAAPMFHMNATISAKT
ALHAGASMVLLPSFDARLYAQAIAKYRVTWLTSVPTMLAMMARERDLIATLDFSSVSHVMMGSAPLTKALVEKVQGIFPG
AAISNAYGTTEAGPGVFGPHPDGLPRPDTAVGYPTEGALAELREGPSPDEGVLYMKNPMLMEGYNNRPEKTAEVMRDGWY
RSGDIMRRDAHGFFHFLGRADDMFVVGGENVWPGEVEKLIERMPGVHQAAVVPVPDDIKGTLPFAFVVKQDGAAIDEAAV
KTFTIANGPAFAHPRFVEFRAAIALSATNKPDRRLLTEEAQQIAAKRRAAGERG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=553, Percent_Identity=25.8589511754069, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI42544132, Length=480, Percent_Identity=27.2916666666667, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI157311624, Length=491, Percent_Identity=24.6435845213849, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI157311622, Length=491, Percent_Identity=24.6435845213849, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI122937307, Length=487, Percent_Identity=25.2566735112936, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI58082049, Length=491, Percent_Identity=24.847250509165, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761345, Length=524, Percent_Identity=24.4274809160305, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI187761343, Length=524, Percent_Identity=24.4274809160305, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI38505220, Length=478, Percent_Identity=23.6401673640167, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI115511026, Length=490, Percent_Identity=23.6734693877551, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8923896, Length=372, Percent_Identity=27.1505376344086, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI253970489, Length=372, Percent_Identity=27.1505376344086, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI13236579, Length=488, Percent_Identity=23.3606557377049, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788107, Length=510, Percent_Identity=27.4509803921569, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI145693145, Length=458, Percent_Identity=27.5109170305677, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=25.3549695740365, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789201, Length=369, Percent_Identity=31.7073170731707, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=24.901185770751, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788595, Length=422, Percent_Identity=26.7772511848341, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786801, Length=507, Percent_Identity=25.6410256410256, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786529, Length=550, Percent_Identity=26.1818181818182, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1790505, Length=544, Percent_Identity=25.1838235294118, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=508, Percent_Identity=24.2125984251968, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=518, Percent_Identity=26.6409266409266, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=511, Percent_Identity=26.8101761252446, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=518, Percent_Identity=26.6409266409266, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=375, Percent_Identity=28.5333333333333, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=555, Percent_Identity=24.5045045045045, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=433, Percent_Identity=27.2517321016166, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=449, Percent_Identity=27.1714922048998, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=546, Percent_Identity=25.0915750915751, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=478, Percent_Identity=26.5690376569038, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=507, Percent_Identity=24.8520710059172, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=431, Percent_Identity=24.1299303944316, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=346, Percent_Identity=25.1445086705202, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=462, Percent_Identity=26.8398268398268, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=462, Percent_Identity=26.8398268398268, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17510401, Length=464, Percent_Identity=23.0603448275862, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=527, Percent_Identity=24.2884250474383, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=508, Percent_Identity=24.4094488188976, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=560, Percent_Identity=24.2857142857143, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21355181, Length=474, Percent_Identity=26.1603375527426, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24581924, Length=488, Percent_Identity=26.4344262295082, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356441, Length=479, Percent_Identity=24.0083507306889, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859661, Length=364, Percent_Identity=27.7472527472527, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI19922652, Length=513, Percent_Identity=23.1968810916179, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356947, Length=444, Percent_Identity=24.3243243243243, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648255, Length=472, Percent_Identity=24.1525423728814, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648253, Length=472, Percent_Identity=24.364406779661, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21358303, Length=451, Percent_Identity=23.059866962306, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24656500, Length=461, Percent_Identity=22.9934924078091, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648260, Length=437, Percent_Identity=24.9427917620137, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648257, Length=348, Percent_Identity=27.8735632183908, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI62472339, Length=381, Percent_Identity=27.2965879265092, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667959, Length=381, Percent_Identity=27.2965879265092, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667955, Length=381, Percent_Identity=27.2965879265092, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130357, Length=313, Percent_Identity=25.2396166134185, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281365686, Length=306, Percent_Identity=24.1830065359477, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653035, Length=441, Percent_Identity=22.9024943310658, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161076582, Length=494, Percent_Identity=21.6599190283401, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57896; Mature: 57765

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSEYTDWTETSVAVSVQLRSRPLWSAFMSWPSRNLGCCFDPSAQPDKVAIIDLRRPEQ
CCCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCCC
PQEITYAALDAACDAVARGLLGRGLTRGDRVGILSLNSAPMIAAYLGIMRAGLVAVPISF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEE
KLARDTVDYIVKDADLRAVFHDHERAATVPDGVLGIDFDAPDGYAALLDHGPFTAIEPQA
EEHHHHHHHHHCCCCHHEEHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCC
REVATILYTSGSTGMPKGVLLSHESQMWALDTAARAGDRSQHRYIVAAPMFHMNATISAK
CEEEEEEEECCCCCCCCCEEEECCCCEEEEHHHHHCCCCCCCEEEEEECEEECCCEEEHH
TALHAGASMVLLPSFDARLYAQAIAKYRVTWLTSVPTMLAMMARERDLIATLDFSSVSHV
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHH
MMGSAPLTKALVEKVQGIFPGAAISNAYGTTEAGPGVFGPHPDGLPRPDTAVGYPTEGAL
HHCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AELREGPSPDEGVLYMKNPMLMEGYNNRPEKTAEVMRDGWYRSGDIMRRDAHGFFHFLGR
HHHHCCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCC
ADDMFVVGGENVWPGEVEKLIERMPGVHQAAVVPVPDDIKGTLPFAFVVKQDGAAIDEAA
CCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHH
VKTFTIANGPAFAHPRFVEFRAAIALSATNKPDRRLLTEEAQQIAAKRRAAGERG
EEEEEEECCCCCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNSEYTDWTETSVAVSVQLRSRPLWSAFMSWPSRNLGCCFDPSAQPDKVAIIDLRRPEQ
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCCC
PQEITYAALDAACDAVARGLLGRGLTRGDRVGILSLNSAPMIAAYLGIMRAGLVAVPISF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEE
KLARDTVDYIVKDADLRAVFHDHERAATVPDGVLGIDFDAPDGYAALLDHGPFTAIEPQA
EEHHHHHHHHHCCCCHHEEHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCC
REVATILYTSGSTGMPKGVLLSHESQMWALDTAARAGDRSQHRYIVAAPMFHMNATISAK
CEEEEEEEECCCCCCCCCEEEECCCCEEEEHHHHHCCCCCCCEEEEEECEEECCCEEEHH
TALHAGASMVLLPSFDARLYAQAIAKYRVTWLTSVPTMLAMMARERDLIATLDFSSVSHV
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHH
MMGSAPLTKALVEKVQGIFPGAAISNAYGTTEAGPGVFGPHPDGLPRPDTAVGYPTEGAL
HHCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AELREGPSPDEGVLYMKNPMLMEGYNNRPEKTAEVMRDGWYRSGDIMRRDAHGFFHFLGR
HHHHCCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCC
ADDMFVVGGENVWPGEVEKLIERMPGVHQAAVVPVPDDIKGTLPFAFVVKQDGAAIDEAA
CCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHH
VKTFTIANGPAFAHPRFVEFRAAIALSATNKPDRRLLTEEAQQIAAKRRAAGERG
EEEEEEECCCCCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]